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1. 发明授权

EP0448609B1 IN VITRO MUTAGENESIS 失效
标题翻译：体外突变。
公开(公告)号：EP0448609B1
公开(公告)日：1993-11-18
申请号：EP90900787.4
申请日：1989-11-21
申请人： DYNAL A.S.
发明人： HORNES, Erik , KORSNES, Lars
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12Q1/6876 , C12N15/102 , C12Q1/6834 , C12Q2563/143
摘要： The method for in vitro mutagenesis of a nucleic acid to produce single stranded DNA containing one or more base alterations includes the steps of: a) preparing an insoluble support carrying a nucleic acid to be mutagenised; b) adding a DNA oligonucleotide primer, which has the desired base alteration(s) but sufficient homology to hybridise with the appropriate site on the said first nucleic acid and allowing the primer to bind thereto; c) hybridising where necessary a further primer corresponding to the end of the nucleic acid at which synthesis is to start and adding a polymerase or reverse transcriptase, and nucleotides to form a DNA strand having the desired alteration(s) and including a ligase to ligate the said DNA strand to the primer; d) removing the first nucleic acid from the synthesised DNA strand; and e) removing said liquid therefrom. Advantageously the nucleic acid to be mutagenised is attached to the insoluble support by hybridisation to a nucleic acid probe attached to said support which probe is covalently attached to said insoluble support. Preferably, the insoluble support consists of magnetic particles which are monodisperse and/or superparamagnetic.

2. 发明授权

EP0446260B1 NUCLEIC ACID PROBES 失效
标题翻译：核酸探针
公开(公告)号：EP0446260B1
公开(公告)日：1994-03-02
申请号：EP90900184.4
申请日：1989-11-21
申请人： DYNAL A.S.
发明人： HORNES, Erik , KORSNES, Lars
IPC分类号： C12Q1/68
CPC分类号： G01N33/54326 , C12N15/1096 , C12Q1/6809 , C12Q1/6813 , C12Q1/6816 , C12Q1/6834 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q2565/519 , C12Q2563/143
摘要： Monodisperse, superparamagnetic particles carrying a plurality of molecules of an oligonucleotide are disclosed and may be used inter alia for sequencing single stranded nucleic acids. The oligonucleotides may be covalently attached or affinity bonded to the particles either by their 3' or 5' termini. The particles have a specific gravity in the range 1.1 to 1.8 and are 1 to 10 microns in diameter. A kit for the isolation and/or processing of target nucleic acid is also disclosed.
摘要翻译：公开了带有多个寡核苷酸分子的单分散性超顺磁颗粒，并且可以用于测序单链核酸。寡核苷酸可以通过其3'或5'末端共价连接或亲和键合至颗粒。颗粒具有1.1至1.8的比重和1至10微米的直径。还公开了用于分离和/或处理靶核酸的试剂盒。

3. 发明授权

EP0444120B1 DETECTION AND QUANTITATIVE DETERMINATION OF RNA AND DNA 失效
标题翻译： RNA和DNA的检测和定量测定
公开(公告)号：EP0444120B1
公开(公告)日：1993-09-01
申请号：EP90900191.9
申请日：1989-11-21
申请人： DYNAL A.S.
发明人： HORNES, Erik , KORSNES, Lars
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q1/6853 , C12Q2565/537 , C12Q2563/143 , C12Q2545/10
摘要： The method for the detection and/or quantitative determination of target RNA or DNA in an analyte sample includes the steps of: a) contacting the analyte with magnetic particles carrying a single stranded 5'-attached DNA probe capable of binding to said RNA or DNA; b) contacting the magnetic particles with an aqueous solution of a reverse transcriptase or polymerase in the presence of labelled nucleotides using the probe as a primer whereby labelled complementary single stranded DNA is formed if said RNA or DNA was present in said analyte; magnetically aggregating the magnetic particles onto a surface and removing said solution; and c) detecting the presence or absence of said label and/or determining the amount of said label. The chain length of complementary single stranded DNA is limited by, for example a predetermined amount of dideoxynucleotides being present at step (b) to limit chain synthesis to a predetermined average length or the probe binding to said DNA or RNA a predetermined distance from a chain terminating point of said DNA or RNA. A kit for performing the method is disclosed.

4. 发明公开

EP0507839A1 METHOD OF SEPARATING HAEMOPOIETIC PROGENITOR CELLS 失效
标题翻译：分离方法用于分离HÄMATOPOEITISCHEN祖细胞。
公开(公告)号：EP0507839A1
公开(公告)日：1992-10-14
申请号：EP91902106.0
申请日：1990-12-28
申请人： DYNAL A.S.
发明人： FUNDERUD, Steinar , SMELAND, Erland, Bremerthun
IPC分类号： C12N5 , A61K35 , C07K16 , C12N15 , C12P21 , G01N33 , C12R1
CPC分类号： A61K35/28 , C07K16/3061
摘要： The invention provides a method of separating haemopoietic progenitor cells (HPC) from within a mixed population of haemopoietic cells, which may contain malignant cells, characterised in that (1) said mixed population is treated with one or more negative selection antibodies reactive with the DP and DQ antigens of the MHC Class II but not with the monomorphic epitope of the DR antigen on the HPC, said negative selection antibody(s) being bound to magnetic particles before or after binding to said cells and the magnetic particles and attached negative selection antibody and cells being removed to leave a negatively selected population of cells including at least HPC, and (2) HPC and any cells associated therewith are treated with a positive selection antibody reactive with an antigen on said HPC, said antibody being bound to magnetic particles before or after binding to cells carrying said antigen, the magnetic particles and attached cells being separated from other cells by magnetic aggregation and the cells of optionally being liberated from the magnetic particles to leave a positively selected population of cells containing said HPC, steps (1) and (2) being effected in either order so that the said positively selected population of cells is the mixed population treated in step (1) or the said negatively selected population of cells are the cells treated in step (2).

5. 发明公开

EP1009761A1 BIOMOLECULES COMPRISING AN ELASTOMERIC PEPTIDE 审中-公开
标题翻译：弹性体肽生物分子含
公开(公告)号：EP1009761A1
公开(公告)日：2000-06-21
申请号：EP98940427.2
申请日：1998-08-28
申请人： DYNAL A.S.
发明人： REIERSEN, Herald,University of Bath , REES, Anthony,University of Bath , KORSNES, Lars
IPC分类号： C07K14/00 , C07K16/10 , A61K38/16 , C07K14/78 , C12N15/62
CPC分类号： C07K14/78 , C07K14/31 , C07K16/1063 , C07K2319/00 , C07K2319/21 , C07K2319/23 , C12N15/62
摘要： The present invention relates to biomolecules containing an inserted amino acid sequence comprising an elastomeric peptide which is inducibly contractible, which on contraction or extension alters the properties or characteristics of the molecule in which it is inserted, particularly biomolecules such as antibodies, nucleic acid molecules encoding such biomolecules and the use of such biomolecules.

6. 发明公开

EP0525034A1 ANTIGEN/ANTI-ANTIGEN CLEAVAGE 失效
标题翻译：抗原/ ANTIANTIGENSPALTUNG。
公开(公告)号：EP0525034A1
公开(公告)日：1993-02-03
申请号：EP91907924.0
申请日：1991-04-09
申请人： DYNAL A.S.
发明人： AFSETH, John , CAIGNAULT, Laurent , FUNDERUD, Steinar , MORTADA, Mohamad 5, rue Pierre et Marie-Curie
IPC分类号： G01N33 , C12N5
CPC分类号： G01N33/686 , G01N33/54326 , G01N33/6857 , Y10S435/975
摘要： L'invention se rapporte à un procédé de dissociation d'une liaison antigène/anti-antigène ou haptène/anti-haptène reliant deux particules, comprenant la réaction de ladite liaison avec un anticorps secondaire, ou fragment de celui-ci, sa combinaison audit anti-antigène ou anti-haptène, ainsi qu'un ensemble kit de mise en application dudit procédé. Le procédé objet de l'invention trouve une application particulière dans la séparation de cellules.

7. 发明公开

EP0483194A1 IgG3 ANTIBODIES WITH SHORTENED HINGE REGION AND A COMPLEMENT ACTIVATION TEST 失效
标题翻译：具有缩短铰链区的IgG3抗体和补体激活试验
公开(公告)号：EP0483194A1
公开(公告)日：1992-05-06
申请号：EP90910699.0
申请日：1990-07-17
申请人： DYNAL A.S.
发明人： MICHAELSEN, Terje , Sandlie, Inger
IPC分类号： A61K39 , C07K16 , C07K19 , C12N5 , C12N15 , C12P21 , C12R1
CPC分类号： C07K16/18 , C07K16/00 , C07K16/44
摘要： L'invention concerne, dans son premier aspect, l'IgG3 (immunoglobuline G3) modifiée contenant des régions humaines constantes, laquelle comporte une région charnière totale plus courte que l'IgG3 humaine. Dans un second aspect, l'invention concerne également un procédé d'analyse d'un anticorps contre un antigène ou haptène spécifique afin de détecter son efficacité dans l'activation du complément chez des espèces animales, consistant à mettre ledit anticorps en contact avec l'antigène ou le haptène immobilisé afin de former un complexe anticorps/antigène ou haptène immobilisés lequel est ensuite mis en contact avec un complèment provenant de l'espèce animale concernée puis à procéder à l'analyse de composant du complément ainsi formé et lié au complexe anticorps/antigène ou anticorps/haptène, de sorte que l'on peut déterminer l'ampleur et la nature de l'activation du complément par l'anticorps dans l'échantillon.
摘要翻译：本发明涉及在其第一方面中，IgG3（免疫球蛋白G3）包含改性的人恒定区，其具有一个总的铰链区比人类IgG3短。在第二方面，本发明还涉及一种方法，用于分析的抗体针对特定抗原或半抗原的检测其在补体活化的动物物种的有效性，包括使所述抗体与所述抗原或半抗原，以形成抗体/抗原或半抗原固定化，然后将其与补体从相关动物物种接触，然后与这样形成的补体成分分析进行，并且结合到复杂的抗体/抗原或抗体/半抗原，从而可以确定样品中抗体对补体激活的程度和性质。

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