专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明公开

EP0952976A1 METHODS AND COMPOSITIONS FOR SENSITIVE AND RAPID, FUNCTIONAL IDENTIFICATION OF GENOMIC POLYNUCLEOTIDES AND USE FOR CELLULAR ASSAYS IN DRUG DISCOVERY 失效
标题翻译： FOR灵敏和快速功能鉴定基因组多核苷酸的方法和组合物及其在细胞试验搜查毒品
公开(公告)号：EP0952976A1
公开(公告)日：1999-11-03
申请号：EP97943625.0
申请日：1997-09-26
申请人： Aurora Biosciences Corporation
发明人： WHITNEY, Michael, A. , NEGULESCU, Paul, A. , CRAIG, Frank , MERE, Lora , FOULKES, Gorden, J.
IPC分类号： C12N15 , C12Q1 , G01N33
CPC分类号： C12N15/1082 , C12N15/1051 , C12Q1/34 , C12Q1/6897 , G01N33/5008 , G01N33/502 , G01N33/5041 , G01N33/505 , G01N33/5073 , G01N2333/986 , G01N2500/00 , G01N2500/10
摘要： The invention provides for methods and compositions for identifying proteins or chemicals that directly or indirectly modulate a genomic polynucleotide and methods for identifying active genomic polynucleotides. Generally, the method comprises inserting a BL (beta-lactamase) expression construct into an eukaryotic genome, usually non-yeast, contained in at least one living cell, contacting the cell with a predetermined concentration of a modulator, and detecting BL activity in the cell.

2. 发明公开

EP0877805A1 TANDEM FLUORESCENT PROTEIN CONSTRUCTS 失效
标题翻译： TANDEM荧光蛋白构建
公开(公告)号：EP0877805A1
公开(公告)日：1998-11-18
申请号：EP97905667.0
申请日：1997-01-31
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , Aurora Biosciences Corporation
发明人： TSIEN, Roger, Y. , HEIM, Roger , CUBITT, Andrew
IPC分类号： C07K14 , C12N1 , C12N15 , C12Q1 , G01N33
CPC分类号： C12Q1/485 , C07K14/43595 , C07K2319/00 , C12Q1/37 , G01N33/542 , G01N2333/43595
摘要： This invention provides tandem fluorescent protein constructs including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.

3. 发明公开

EP1175508A1 OPTICAL PROBES AND ASSAYS 有权
标题翻译：用于测量蛋白质磷酸化的光学传感器和化验
公开(公告)号：EP1175508A1
公开(公告)日：2002-01-30
申请号：EP00932098.7
申请日：2000-05-05
申请人： Aurora Biosciences Corporation
发明人： POLLOK, Brian, A. , HAMMAN, Brian, D. , RODEMS, Steven, M. , MAKINGS, Lewis, R.
IPC分类号： C12Q1/37 , C12Q1/42 , C12Q1/48 , C07K14/435
CPC分类号： G01N33/6842 , C12Q1/37 , C12Q1/42 , C12Q1/48 , C40B30/04 , C40B40/10 , C40B60/12 , G01N33/542 , G01N33/582 , G01N33/6803 , G01N2333/43595 , G01N2333/9121
摘要： This invention provides an optical probe useful as an optical probe or sensor of post translational type modifications, such as phosphorylation. The invention comprises a polypeptide moiety, which contains a recognition motif for a post translational type activity, and a protease site, which is coupled to a probe moiety. Modification of the polypeptide, by the post translational type activity, results in a modulation of the rate at which a protease cleaves the polypeptide which is sensed by a measurable change in at least one optical property of the optical probe upon cleavage. The present invention also includes a recombinant nucleic acid molecule that encodes an optical probe and a vector and host cell or library of cells that include the recombinant nucleic acid molecule. The optical probe can be used in methods to determine whether a sample, including a cell or a sample from an organism, contains a post-translational type modification activity. Such methods can also be used to determine whether a test chemical modulates the activity of a modifying activity, and thus can be used to identify therapeutic compositions. The identification of such therapeutic compositions can be automated using a system that includes an optical probe.

4. 发明公开

EP1137489A1 FLUID DISPENSER AND DISPENSING METHODS 有权
标题翻译： DEVICE液体分配和供给方式
公开(公告)号：EP1137489A1
公开(公告)日：2001-10-04
申请号：EP99965225.8
申请日：1999-12-10
申请人： Aurora Biosciences Corporation
发明人： SASAKI, Glenn, C.
IPC分类号： B01L3/02 , G01N35/10
CPC分类号： G01N35/1065 , B01L3/0268 , B01L2400/0433 , G01N35/10 , G01N2035/00237 , G01N2035/1034 , Y10T436/2575
摘要： A fluid dispenser comprises a fluid chamber having two actuators coupled thereto. One of the actuators damps a fluid response of the other. The fluid chamber may comprise a cylindrical capillary, and the actuators may comprise spaced cylindrical piezoelectric elements.

5. 发明公开

EP1081495A1 Quenchers for fluorescence assays 无效
标题翻译： LöscherfürFluoreszenzassays
公开(公告)号：EP1081495A1
公开(公告)日：2001-03-07
申请号：EP99117221.4
申请日：1999-09-01
申请人： Aurora Biosciences Corporation
发明人： Knapp, Tom , Zlokarnik, Gregor , Negulescu, Paul , Tsien, Roger Y. , Rink, Tim
IPC分类号： G01N33/542
CPC分类号： G01N33/542
摘要： The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
摘要翻译：本发明提供了使用至少一种光子产生剂和至少一种光子还原剂（例如基于染料的光子还原剂）来减少来自样品的不希望的光发射的方法。本发明进一步提供了用至少一种光子产生剂和至少一种碰撞猝灭剂减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了用至少一种光子产生剂和至少一种猝灭剂（诸如电子猝灭剂）减少来自样品（例如，生物化学或细胞样品）的不希望的光发射的方法。本发明还提供了使用光子还原剂在荧光测定中筛选测试化学品的系统和方法。本发明还提供了用于实施这些方法的组合物，药物组合物和试剂盒。

6. 发明公开

EP1004870A1 Liquid barriers for assays 审中-公开
标题翻译： FlüssigkeitsbarrierefürAssays
公开(公告)号：EP1004870A1
公开(公告)日：2000-05-31
申请号：EP99122323.1
申请日：1999-11-09
申请人： Aurora Biosciences Corporation
发明人： Sasaki, Glenn , Coassin, Peter J. , Pham, Andrew A. , Harootunian, Alec , Mere, Lora , Cubitt, Andrew B.
IPC分类号： G01N1/28 , G01N33/50
CPC分类号： B01L3/00 , G01N2035/00287
摘要： The present invention provides liquid barriers that can reduce the evaporation of a liquid sample and is compatible with living cells. The liquid barriers of the present invention replace the need for mechanical barriers, such as lids and covers, for use with assay containers, such as multi-well platforms. The use of such liquid barriers rather than mechanical barriers reduces the need for robotics in automated screening procedures and thus allows for higher throughput in such procedures.
摘要翻译：本发明提供了可以减少液体样品的蒸发并且与活细胞相容的液体屏障。本发明的液体屏障代替了对诸如多孔平台的测定容器使用的机械屏障（例如盖和盖）的需要。使用这种液体屏障而不是机械障碍减少了机器人在自动筛选过程中的需要，因此允许在这种程序中更高的生产量。

7. 发明公开

EP1262478A2 Methods and compositions for determining cellular response profiles 失效
标题翻译： Verfahren und Zusammensetzungen zur Bestemmung vonzellülarenResponse-Profile
公开(公告)号：EP1262478A2
公开(公告)日：2002-12-04
申请号：EP02010958.3
申请日：1997-09-26
申请人： Aurora Biosciences Corporation
发明人： Whitney, Michael A. , Negulescu, Paul A. , Craig, Frank , Mere, Lora , Foulkes, Gorden J.
IPC分类号： C07D221/02 , C07D215/12 , C12Q1/70 , C12Q1/68 , C12Q1/08 , C12P21/06 , C12N15/00 , C12N9/14 , C12N9/84 , C12N9/86 , C12Q1/00 , G01N33/50
CPC分类号： C12N15/1082 , C12N15/1051 , C12Q1/34 , C12Q1/6897 , G01N33/5008 , G01N33/502 , G01N33/5041 , G01N33/505 , G01N33/5073 , G01N2333/986 , G01N2500/00 , G01N2500/10
摘要： The invention provides methods for determining a cellular response profile for a target or chemical, for screening compounds for activity as an activator or inhibitor of a target and for developing a cell sensor panel. Furthermore, cell sensor panels are provided. Generally, the methods comprise inserting a BL (beta-lactamase) expression construct via a viral vector into an non-yeast eukaryotic genome, contained in at least one living cell, contacting the cell with a predetermined concentration of a modulator, and detecting BL activity in the cell.
摘要翻译：本发明提供用于确定靶或化学物质的细胞反应谱的方法，用于筛选作为靶的激活剂或抑制剂的活性的化合物和用于开发细胞传感器面板的方法。此外，提供了电池传感器面板。通常，所述方法包括通过病毒载体将BL（β-内酰胺酶）表达构建体插入包含在至少一个活细胞中的非酵母真核基因组中，使细胞与预定浓度的调节剂接触，并检测BL活性在细胞中

8. 发明公开

EP1255853A2 METHODS OF PROTEIN DESTABILIZATION AND USES THEREOF 审中-公开
标题翻译：蛋白的方法及其用途失稳
公开(公告)号：EP1255853A2
公开(公告)日：2002-11-13
申请号：EP01907018.4
申请日：2001-02-02
申请人： Aurora Biosciences Corporation
发明人： STACK, Jeffrey, H. , WHITNEY, Michael , CUBITT, Andrew, B. , POLLOK, Brian, A.
IPC分类号： C12Q1/37 , C12Q1/34 , G01N33/532 , C12N15/00 , G01N33/50 , G01N33/542
CPC分类号： C12Q1/485
摘要： This invention is directed towards methods of destabilizing proteins in living cells, and their use for the development of novel assays. In one embodiment, the invention comprises the use of non-cleavable multimerized ubiquitin fusion proteins to destabilize a target protein, such as a reporter moiety. In one aspect of this method the constructs also comprises a linker that operatively couples the reporter moiety to the multimerized ubiquitin fusion protein. In this embodiment, enzymatic modification of the linker results in a modulation of the coupling of the reporter protein to the multimerized ubiquitin domains resulting in a change in the stability of the reporter moiety. The level of the reporter moiety in the cell can then be used as a measure of the enzymatic activity in the cell. In another embodiment the invention provides for a generalized way of coordinately regulating the cellular concentration of a plurality of target proteins. In one aspect of this method, the target proteins are operatively coupled to a ubiquitin fusion protein via linker containing a protease cleavage site. Cleavage of the linker by a protease results in uncoupling of the target protein from the multimerized ubiquitin construct, and results in an increase in the stability and concentration of the target protein.

9. 发明公开

EP1224474A1 MULTIWELL SCANNER AND SCANNING METHOD 审中-公开
标题翻译： MEHRFACHLOCHPLATTENSCANNER UND ABTASTVERFAHREN
公开(公告)号：EP1224474A1
公开(公告)日：2002-07-24
申请号：EP00975255.1
申请日：2000-10-12
申请人： Aurora Biosciences Corporation
发明人： VUONG, T., Minh
IPC分类号： G01N35/02 , G01N21/76
CPC分类号： G01N21/253 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00707 , B01J2219/0072 , B01L2300/0893 , C40B60/14 , G01N21/76 , G01N35/028
摘要： A multiwell plate scanner comprises a detector for measuring an attribute of a sample which is scanned continuously over wells of a multiwell plate. A signal obtained during the scan may be sampled and digitized based on detector position over the multiwell plate. The scanner is also disclosed for scanning microarrays, bio-chips and areas of samples not having physical separations. The scanner may be used in a high throughput screening system comprising a storage and retrieval module, a sample distribution module, a reagent distribution module, and a detector which incorporates the scanner. The screening system may further comprise a transport module and a data processing and integration module for transporting samples between the components of the system and for controlling system operation. In a further aspect, the present invention comprises methods and apparatus to compensate for variations, inconsistencies and other differences in the dimensions, configuration and/or materials of the platform in which an assay is performed. Another aspect of the invention is a system and method for performing an assay to detect the effect of a reagent on a target. The present invention is also directed to compositions and therapeutics identified by the disclosed methods. A further aspect of the present invention is a method of testing a therapeutic for therapeutic activity and toxicology by identifying a compound using a method of the present invention and monitoring the toxicology and efficacy of the therapeutic in an in vivo model.
摘要翻译：多孔板扫描器包括用于测量在多孔板的孔上连续扫描的样品的属性的检测器。扫描期间获得的信号可以基于多孔板上的检测器位置进行采样和数字化。还公开了用于扫描微阵列，生物芯片和不具有物理分离的样品区域的扫描仪。扫描器可以用于包括存储和检索模块，样品分配模块，试剂分配模块和结合扫描仪的检测器的高通量筛选系统中。筛选系统还可以包括传输模块和数据处理和集成模块，用于在系统的组件之间传送样本并用于控制系统操作。在另一方面，本发明包括用于补偿其中进行测定的平台的尺寸，构型和/或材料的变化，不一致性和其它差异的方法和装置。本发明的另一方面是用于进行测定以检测试剂对靶标的影响的系统和方法。本发明还涉及通过所公开的方法鉴定的组合物和治疗剂。本发明的另一方面是通过使用本发明的方法鉴定化合物并在体内模型中监测治疗剂的毒理学和功效来测试治疗活性和毒理学治疗剂的方法。

10. 发明公开

EP0973040A3 Detector and screening device for ion channels 有权
标题翻译：检测器和用于离子通道筛选设备
公开(公告)号：EP0973040A3
公开(公告)日：2000-03-15
申请号：EP99113933.8
申请日：1999-07-16
申请人： Aurora Biosciences Corporation
发明人： Tsien, Roger Y. , Coassin, Peter J. , Pham, Andrew A. , Harootunian, Alec Tate , Vuong, Minh
IPC分类号： G01N35/02 , G01N21/64 , G01N21/25 , G02B6/04
CPC分类号： G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6478 , G01N2021/6484 , G01N2201/0639
摘要： The invention provides for a detector assembly, fiber assembly and screening system for optical measurements.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式