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    • 10. 发明授权
    • ENZYMATIC METHOD FOR (R)-SELECTIVE AMINATION
    • (R) - 亲核胺化的酶法
    • EP2593556B1
    • 2017-08-30
    • EP11733837.6
    • 2011-07-14
    • DPx Holdings B.V.
    • SCHÜRMANN, MartinPEETERS, Wijnand Peter HelenaSMEETS, Natascha Hubertina JohannesSCHWAB, HelmutSTEINER, KerstinLYPETSKA, Kateryna MykolayivnaSTROHMEIER, Gernot
    • C12P13/00C12N9/10C12N1/14C12N1/20C12N15/54
    • C12P13/001C12N9/1096C12Y206/01
    • The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c], from the corresponding ketones of general formula [1][a], by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusilllum, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkholderia sp., Burkholderia tropica, Cosmospora episphaeria, and Fusarium oxysporum.
    • 本发明涉及通过使用新型转氨酶从相应的通式[1] [a]的酮酶合成通式[1] [c]的对映体富集的(R) - 胺的方法。 这些新型转氨酶选自两个不同的组:从具有本文具体说明的序列的约20种蛋白质的组中选出,或选自具有转氨酶活性的蛋白质组中的一组蛋白质,并且从选自下列的微生物中分离:Rahnella aquatilis,Ochrobactrum anthropi,苍白杆菌小麦,苜蓿morelense,Curtobacterium属pusilllum,淡紫拟青霉,微ginsengisoli,微trichothecenolyticum,假单胞菌citronellolis,耶尔森kristensenii,无色spanius,无色insolitus,偶发分枝杆菌,结核分枝frederiksbergense,结核分枝骶骨,结核分枝fluoranthenivorans,鼻疽菌,类鼻疽tropica ,Cosmospora episphaeria和Fusarium oxysporum。