会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 6. 发明公开
    • NUCLEOTIDE SEQUENCING METHOD
    • 核苷酸测序法
    • EP0777747A1
    • 1997-06-11
    • EP95929169.0
    • 1995-08-21
    • The Solicitor of the Affairs of Her Majesty's Treasury
    • TULLY, GillianSULLIVAN, Kevin
    • C12Q1
    • C12Q1/6858C12Q1/6869
    • A method is provided for determining the identity of at least two discrete single nucleotide bases each adjacent to a predetermined nucleotide base sequence in a target sample comprising one or more types of polynucleotide chain, the method comprising incorporating a capture group into the target sample and immobilising the target sample by means of the capture group; mixing the target sample with (i) nucleotide primers which are complementary to the characterised base sequences such that they anneal thereto at positions adjacent to the bases to be identified and (ii) at least two types of dideoxynucleoside triphosphate (ddNTP), each type labelled with a distinguishable fluorescent group, and (iii) a nucleotide chain extending enzyme such that ddNTPs complementary to the bases to be identified are incorporated into the nucleotide primers; eluting the extending nucleotide primers, separating the types of extended nucleotide primer on basis of size or charge and identifying the ddNTPs incorporated into each type of nucleotide primer by reference to the fluorescent characteristics associated with the distinguishable groups. Primers and a test kit are provided for use in performance of the method.