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    • 8. 发明公开
    • METHODS FOR IMPROVING MALIC ACID PRODUCTION IN FILAMENTOUS FUNGI
    • 提高丝状真菌中的苹果酸生产的方法
    • EP3103876A1
    • 2016-12-14
    • EP16176171.3
    • 2010-08-27
    • Novozymes Inc.
    • Brown, StephenLuttringer, SherylYaver, DebbieBerry, Alan
    • C12P7/46C07K14/38C12N1/14C12N9/04
    • C12P7/46C07K14/38C12N15/80
    • The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.
    • 本发明涉及生产C4二羧酸的方法,其包括:(a)培养丝状真菌宿主细胞,所述细胞包含选自以下的多核苷酸:编码C4二羧酸转运蛋白的异源第一多核苷酸,编码 苹果酸脱氢酶和编码丙酮酸羧化酶的异源第三多核苷酸; 其中与在相同条件下培养时不含异源多核苷酸的丝状真菌宿主细胞相比,丝状真菌宿主细胞能够分泌更高水平的C4二羧酸; 和(b)回收C4二羧酸。 本发明还涉及增加C4二羧酸生产的方法,丝状真菌宿主细胞和苹果酸脱氢酶变体。