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5. 发明公开

EP3030903A2 METHOD FOR THE ANALYSIS OF IMMUNOREACTIVITY, AND A DEVICE SUITABLE FOR CARRYING OUT THE METHOD 有权
标题翻译： VERFAHREN ZUR免责声明：VORRICHTUNG ZURDURCHFÜHRUNGDIESES VERFAHRENS
公开(公告)号：EP3030903A2
公开(公告)日：2016-06-15
申请号：EP14792541.6
申请日：2014-08-07
申请人： Eötvös Loránd Tudományegyetem , Magyar Tudományos Akadémia , Diagnosticum Gyártó És Forgalmazó
发明人： PRECHL, József , PAPP, Krisztián , SZITTNER, Zoltán
IPC分类号： G01N33/50 , B01L3/00 , G01N33/543
CPC分类号： G01N33/54386 , B01L3/502746 , B01L3/502761 , B01L2200/0668 , B01L2200/10 , B01L2300/0636 , B01L2300/0663 , B01L2300/0819 , B01L2300/0858 , B01L2300/0864 , B01L2300/0867 , B01L2400/086 , G01N33/5005
摘要： The invention relates to a capillary channel, which comprises a trap section (1) having a coating, and further comprises a collector section (2) having a volume which is at least as large as that of the trap section (1), and a reader section (3) adjacent to the collector section (2), wherein the reader section (3) optionally comprises one or more physical and/or chemical sensors adapted for measuring a flow rate and/or one or more markings for visual reading of a liquid front position, and wherein the collector section (2) is located in the part between the trap section (1) and the reader section (3) of the capillary channel. The invention also provides a capillary channel system, which comprises at least one measuring channel, which measuring channel is a capillary channel according to the invention, wherein the coating comprises an antigen, and the system comprises at least one negative control channel and optionally at least one positive control channel, and optionally at least one sample distributing part which communicates with the channels. The invention also provides a method for the analysis of immunoreactivity on an antigen array, wherein an antigen array comprising one or more antigens and optionally one or more reference substances is contacted with a sample, and the immune complexes formed by the one or more antigens and one or more components of the sample which may be captured by the antigens are detected by cells, wherein for the cells the sample serves as a source, or the cells are from a source other than the sample. The method is preferably carried out with a device according to the invention. The invention also encompasses kits and uses.
摘要翻译：本发明涉及一种毛细通道，其包括具有涂层的捕集器部分（1），并且还包括具有至少与捕集部分（1）的体积一样大的体积的收集器部分（2），以及读取器部分（3），其中读取器部分（3）可选地包括适于测量流速的一个或多个物理和/或化学传感器和/或用于视觉读取的一个或多个标记液体前部位置，并且其中收集器部分（2）位于陷阱部分（1）和毛细通道的读取器部分（3）之间的部分中。本发明还提供一种毛细通道系统，其包括至少一个测量通道，该测量通道是根据本发明的毛细管通道，其中该涂层包含抗原，并且该系统包含至少一个阴性对照通道，一个正控制通道，以及可选的至少一个与通道通信的样品分配部件。本发明还提供了一种用于分析抗原阵列上的免疫反应性的方法，其中包含一种或多种抗原和任选的一种或多种参考物质的抗原阵列与样品接触，并且由一种或多种抗原形成的免疫复合物和可以由细胞检测可被抗原捕获的样品的一种或多种组分，其中对于细胞，样品用作来源，或者细胞来自除样品之外的来源。该方法优选用根据本发明的装置进行。本发明还包括试剂盒和用途。

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