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81. 发明授权

EP1137807B1 METHODS, KITS AND COMPOSITIONS FOR THE IDENTIFICATION OF NUCLEIC ACIDS ELECTROSTATICALLY BOUND TO MATRICES 有权
标题翻译：方法，REAGENTIENSÄTZE和组合物用于识别静态到相关核酸DIES
公开(公告)号：EP1137807B1
公开(公告)日：2004-09-08
申请号：EP99960659.3
申请日：1999-12-08
申请人： Boston Probes, Inc.
发明人： JOHANSEN, Jack, T. , HYLDIG-NIELSEN, Jens, J. , FIANDACA, Mark, J. , COULL, James, M.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6834 , C12Q1/6837 , C12Q2563/131 , C12Q2525/107
摘要： This invention pertains to methods, kits and compositions suitable for the detection, identification and/or quantitation of nucleic acids which are electrostatically immobilized to matrices using non-nucleotide probes which sequence specifically hybridize to one or more target sequences of the nucleic acid but do not otherwise substantially interact with the matrix. Once the nucleic acid is immobilized, the detectable non-nucleotide probe/target sequence complex, formed before or after the immobilization of the nucleic acid, can be detected, identified or quantitated under a wide range of assay conditions as a means to detect, identify or quantitate the target sequence in the sample. Because it is reversibly bound, the non-nucleotide probe/target sequence can optionally be removed from the matrix for detecting, identifying or quantitating the target sequence in the sample. Because the non-nucleotide probe/target sequence is protected against degradation, it is another advantage of this invention that the sample can be treated with enzymes which degrade sample components, either before or after the nucleic acid is bound to the matrix, in order to 'clean up' the sample (e.g. a complex biological sample such as a cell lysate) and thereby improve the detection, identification or quantitation of the target sequence in the sample. The methods, kits and compositions of this invention are therefore particularly well suited for the analysis, and particularly single point mutation analysis, in a particle assay, in an array assay, in a nuclease digestion/protection assay and/or in a line assay format. When utilized in combination with non-nucleotide 'Beacon' probes, the invention is particularly well suited for use in a self-indicating assay format.

82. 发明公开

EP1412517A2 METHODS, KITS AND COMPOSITIONS OF COMBINATION OLIGOMERS 有权
标题翻译： KOMBINATIONSOLIGOMERE相关方法，试剂盒和组合物
公开(公告)号：EP1412517A2
公开(公告)日：2004-04-28
申请号：EP02719163.4
申请日：2002-03-09
申请人： Boston Probes, Inc.
发明人： COULL, James, M. , FIANDACA, Mark, J. , KRISTJANSON, Mark, D. , HYLDIG-NIELSEN, Jens, J. , CREASEY, Theresa, M.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C07B2200/11 , C40B20/04 , C40B30/04 , C40B40/00 , C40B40/06 , Y10S435/81 , C12Q2565/137 , C12Q2525/107 , C12Q2531/113
摘要： This invention pertains to the field of combination oligomers, including the block synthesis of combination oligomers in the absence of a template, as well as related methods, kits, libraries and other compositions.

83. 发明公开

EP1385932A2 NUCLEIC ACID ANALYSIS BY MASS DIFFERENTIABLE OLIGOMERS 审中-公开
标题翻译： NUCLEIC检查散装每个UNTERSCHEIBARE低聚物
公开(公告)号：EP1385932A2
公开(公告)日：2004-02-04
申请号：EP02735871.2
申请日：2002-04-24
申请人： Erasmus Universiteit Rotterdam
发明人： GROSVELD, Frank,Erasmus Universiteit Rotterdam , PHILIPSEN, Jacobus N. J. , LUIDER, Theo
IPC分类号： C12G1/00
CPC分类号： C12Q1/6811 , C12Q1/6834 , C12Q2563/167 , C12Q2525/107 , C12Q2523/308
摘要： The invention comprises a method for constructing a repertoire of oligomers differentiable by mass, providing a heterogeneous pool of monomers, wherein said monomers are modified by addition of one or more of a selection of mass labels; optionally, providing a heterogeneous pool of unlabelled monomers; determining the monomer sequences of the oligomers to be represented in the repertoire and calculating the number and nature of the mass labels to be incorporated into each monomer such that each oligomer differs in mass; and assembling a plurality of labelled monomers and, optionally, one or more unlabelled monomers, to form the oligomers.

84. 发明公开

EP1335933A2 PNA ANALOGUES 审中-公开
标题翻译： PNA类似物
公开(公告)号：EP1335933A2
公开(公告)日：2003-08-20
申请号：EP01997499.7
申请日：2001-11-23
申请人： Pantheco A/S
发明人： NIELSEN, Peter, E. , PÜSCHL, Ask
IPC分类号： C07K14/00 , C07K19/00 , C12Q1/68 , A61K48/00
CPC分类号： C12N15/113 , A61K48/00 , C07K14/003 , C12N2310/318 , C12N2310/3181 , C12N2310/323 , C12N2310/345 , C12Q2525/107
摘要： The present invention concerns peptide nucleic acid (PNA) sequences, which are modified in order to obtain novel PNA molecules with enhanced properties. The peptide nucleic acid (PNA) oligomers are characterized in that the single units of the oligomer consists of different amino acid backbones selected from aminoethyl-glycine (aeg), aminoethylprolyl (aep), aminoethylpyrrolidine (pyr) or an amino acid. More over is modified PNA conjugate of formule (I): Q - L - PNA (I) claimed, wherein L is a linker or a bond; Q is a peptide and PNA is the peptide nucleic acid oligomer.
摘要翻译：本发明涉及肽核酸（PNA）序列，其被修饰以获得具有增强性质的新型PNA分子。肽核酸（PNA）寡聚体的特征在于寡聚体的单个单元由选自氨乙基 - 甘氨酸（aeg），氨乙基脯氨酰（aep），氨乙基吡咯烷（pyr）或氨基酸的不同氨基酸主链组成。（I）的修饰的PNA缀合物：其中L是接头或键; Q是肽，PNA是肽核酸寡聚物。

85. 发明公开

EP1198594A2 POLYMERASE EXTENSION AT 3' TERMINUS OF PNA-DNA CHIMERA 有权
标题翻译：通过聚合酶的手段甲PNA-DNA嵌合体到它的3“末端EXTENSION
公开(公告)号：EP1198594A2
公开(公告)日：2002-04-24
申请号：EP00953941.2
申请日：2000-08-09
申请人： Applera Corporation
发明人： EGHOLM, Michael , CHEN, Caifu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6853 , C12Q1/6869 , C12Q2525/107 , C12Q2537/143
摘要： The invention provides methods and a kit for primer extension of PNA-DNA chimera from template nucleic acids using polymerases, nucleotide 5'-triphosphates, and primer extension reagents. Structural requirements of the chimera for primer extension include 5 to 15 contiguous PNA monomer units, 3 or more contiguous nucleotides, and a 3' hydroxyl terminus. The chimera and/or a nucleotide is labelled with the fluorescent dyes or other labels. The methods include DNA sequencing, DNA fragment analysis, reverse transcription, mini-sequencing, chromosome labelling, amplification, and single nucleotide polymorphism (SNP) detection.

86. 发明公开

EP1155152A1 METHODS AND KITS FOR SCREENING NUCLEIC ACID DUPLEX STABILITY 审中-公开
标题翻译：方法和系统稳定性NUCLEIC双链的筛选试验
公开(公告)号：EP1155152A1
公开(公告)日：2001-11-21
申请号：EP99967559.8
申请日：1999-12-23
申请人： RUTGERS, THE STATE UNIVERSITY
发明人： BRESLAUER, Kenneth, J. , GELFAND, Craig, A. , PLUM, G., Eric
IPC分类号： C12Q1/68 , G01N25/20 , G01N1/18 , G01N31/16 , G01N24/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6818 , C12Q1/6827 , C12Q2565/101 , C12Q2527/107 , C12Q2525/107 , C12Q2565/628 , C12Q2537/161 , C12Q2537/119 , C12Q2527/101 , C12Q2525/101
摘要： Simple methods and kits for determining the thermodynamic stability of nucleic acid duplexes and single polynucleotide polymorphisms via competitive equilibria are provided.

87. 发明公开

EP1121463A1 IMPROVED HYBRIDISATION ASSAY IN WHICH EXCESS PROBE IS DESTROYED 审中-公开
标题翻译：改进的杂交试验，其中过探测降级
公开(公告)号：EP1121463A1
公开(公告)日：2001-08-08
申请号：EP99949210.1
申请日：1999-10-12
申请人： Zetatronics Limited
发明人： HARBRON, Stuart
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q1/6804 , C12Q2563/125 , C12Q2531/113 , C12Q2527/119 , C12Q2565/531 , C12Q2525/107 , C12Q2521/307 , C12Q2522/101
摘要： The present invention provides a method for detecting a single-stranded target nucleic acid comprising the steps of: a) forming a hybrid between a target nucleic acid and a nucleic acid probe, said nucleic acid probe labelled with an enzyme reagent which hydrolyses single-stranded nucleic acid but is substantially without effect on double-stranded nucleic acid, said hybrid formed under conditions of pH which are outside the activity range of said enzyme reagent; b) adjusting said pH to a value within the activity range of said enzyme reagent, whereby said enzyme reagent substantially hydrolyses any single-stranded nucleic acid present; and c) contacting said hybrid with a detection reagent to detect the hybrid, characterised by, prior to step (c), bringing the nucleic acid probe or hybrid into contact with a solid support to attach it thereto or bringing the nucleic acid probe or hybrid into contact with a capture reagent, optionally linked to a solid support, to capture the nucleic acid probe or hybrid; and washing the capture reagent or solid support on which the hybrid is immobilised with a washing fluid while the capture reagent or solid support is contained within a vessel that is adapted to retain the capture reagent or solid support but not to retain fluid in which the capture reagent or solid support is dispersed, whereby material which has not been captured by the capture reagent or otherwise immobilised on a solid support is eluted from the vessel.

88. 发明公开

EP1006200A3 Methods for producing selected interstrand cross-links in nucleic acids and applications thereof 有权
标题翻译：一种用于处理在核酸和它们的用途制备的分子间选择
公开(公告)号：EP1006200A3
公开(公告)日：2000-10-11
申请号：EP99204141.8
申请日：1999-12-03
申请人： Kreatech Biotechnology B.V.
发明人： Volkers, Herman , Houthoff, Hendrik Jan , Heetebrij, Robert Jochem , Van Gijlswijk, Robertus Petrus Maria , Tanke, Hendrikus Johannes , Raap, Anton Klaas
IPC分类号： C12Q1/68 , C07K2/00 , C07H23/00 , C07H21/00 , C07F15/00
CPC分类号： C12Q1/6832 , C07H21/04 , C12Q1/6816 , C12Q1/6841 , C12Q1/686 , C12Q2563/137 , C12Q2523/101 , C12Q2525/151 , C12Q2525/107 , C12Q2565/102 , C12Q2525/186
摘要： The invention provides methods and means for generating interstrand cross-links in nucleic acid at certain specific locations in said nucleic acid. Said certain specific locations in said nucleic acid can be selected from other locations through hybridising nucleic acid present in said selected location with complementary nucleic. In one aspect the invention provides a method for providing at least one selected sequence in a nucleic acid with interstrand cross-links comprising hybridising at least one selected single strand sequence with a complementary single strand nucleic acid wherein said selected sequence or said complementary nucleic acid or both comprise a cross-linking agent. The means and methods of the invention may be used in and beneficial for a wide variety of applications, such as the generation of nucleic acid probes and the treatment of diseases such as cancer.

89. 发明公开

EP1012335A1 METHODS AND COMPOSITIONS FOR DETECTION OR QUANTIFICATION OF NUCLEIC ACID SPECIES 审中-公开
标题翻译：借鉴，检测和定量NUCLEIC物种的组成
公开(公告)号：EP1012335A1
公开(公告)日：2000-06-28
申请号：EP98940915.6
申请日：1998-08-14
申请人： HYSEQ, INC.
发明人： DRMANAC, Radoje , DRMANAC, Snezana , BAIDYA, Narayan
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6837 , B01J2219/00317 , B01J2219/00511 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , C12Q1/6874 , C40B40/06 , C40B60/14 , C12Q2525/107 , C12Q2565/518
摘要： The present invention provides a method for detecting a target nucleic acid species using an array of probes affixed to a substrate and a plurality of labeled probes. The invention also relates to oligonucleotide probes attached to discrete particles wherein the particles can be grouped into a plurality of sets based on a physical property. A different probe is attached to the discrete particles of each set, and the identity of the probe is determined by identifying the discrete particles from their physical property. The invention further relates to methods using agents which destabilize the binding of complementary polynucleotide strands (decrease the binding energy) or increase stability of binding between complementary polynucleotide strands (increase the binding energy). The figure is an illustration of an apparatus for mass producing probe arrays.

90. 发明公开

EP1006199A1 Applications with and methods for producing selected interstrand crosslinks in nucleic acid 审中-公开
标题翻译：在核酸链和它们的使用所选择的位置，用于生产环节的方法
公开(公告)号：EP1006199A1
公开(公告)日：2000-06-07
申请号：EP98204094.1
申请日：1998-12-03
申请人： Kreatech Biotechnology B.V.
发明人： The designation of the inventor has not yet been filed
IPC分类号： C12Q1/68 , C07H23/00 , C07H21/00 , C07F15/00
CPC分类号： C12Q1/6832 , C07H21/04 , C12Q1/6816 , C12Q1/6841 , C12Q1/686 , C12Q2563/137 , C12Q2523/101 , C12Q2525/151 , C12Q2525/107 , C12Q2565/102 , C12Q2525/186
摘要： The invention provides methods and means for generating interstrand cross-links in nucleic acid at certain specific locations in said nucleic acid. Said certain specific locations in said nucleic acid can be selected from other locations through hybridising nucleic acid present in said selected location with complementary nucleic. In one aspect the invention provides a method for providing at least one selected sequence in a nucleic acid with interstrand cross-links comprising hybridising at least one selected single strand sequence with a complementary single strand nucleic acid wherein said selected sequence or said complementary nucleic acid or both comprise a cross-linking agent. The means and methods of the invention may be used in and beneficial for a wide variety of applications, such as the generation of nucleic acid probes and the treatment of diseases such as cancer.
摘要翻译：本发明提供了方法和装置，用于产生在核酸在某些所述核酸的特定位置间海滩交联。在所说的核酸可以从其他位置通过混合在说伊辛核酸存在被选择的某些特定位置选择的位置与互补核酸。在一个方面，本发明提供一种用于在核酸与间海滩交联，其包括混合伊辛提供至少一个所选择的序列的方法的至少一个选择的单海滩序列与互补的单海滩核酸worin所述选择的序列或所述互补的核酸或都包括一个交联剂。的手段和本发明的方法可以在使用和用于各种应用，：如核酸探针的产生和疾病的治疗是有益的：如癌症。

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