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81. 发明公开

EP3204489A1 METHODS OF DIFFERENTIATING STEM CELLS INTO LIVER CELL LINEAGES 审中-公开
标题翻译：将干细胞分化为肝细胞系的方法
公开(公告)号：EP3204489A1
公开(公告)日：2017-08-16
申请号：EP15849456.7
申请日：2015-10-08
申请人： Agency for Science, Technology and Research
发明人： ANG, Lay Teng , LOH, Kyle M. , LIM, Bing
IPC分类号： C12N5/071 , C12N5/074 , A61K35/407 , G01N33/00 , A61P1/16
CPC分类号： A61K35/407 , C12N5/00 , C12N5/0603 , C12N5/067 , C12N5/0672 , C12N2500/38 , C12N2500/44 , C12N2501/00 , C12N2501/01 , C12N2501/115 , C12N2501/155 , C12N2501/16 , C12N2501/237 , C12N2501/33 , C12N2501/385 , C12N2501/39 , C12N2501/415 , C12N2501/42 , C12N2501/727 , C12N2506/02 , G01N33/00
摘要： The present disclosure provides methods and kits for the differentiation of stem cells into relevant liver cell lineages, as well as methods of using the relevant liver cell lineages in screening for a cellular response, a phenotype and in the treatment of a condition. In one embodiment, stem cells are first differentiated into cells of the definitive endoderm lineage, which are differentiated into posterior foregut (PFG) lineage cells by one or more of retinoic acid activators and/or one or more inhibitors of transforming growth factor-β (TGFβ). An additional embodiment provides a method for the differentiation of posterior foregut lineage cells into liver bud progenitors (LB) by one or more activators of TGFβ signalling, and/or one or more modulators of Wnt signalling, and/or one or more activators of cyclic AMP/PKA signaling; and a further embodiment provides a method for the differentiation of liver bud progenitors into hepatic progenitors by one or more inhibitors of TGFβ signalling and/or fibroblast growth factor (FGF) inhibitors and/or one or more Notch inhibitors. Another embodiment discloses the differentiation of hepatic progenitors into hepatocyte-like cells or perivenous hepatocyte-like cells by one or more of Notch inhibitors and/or activators of glucocorticoid signalling and/or one or more activators of insulin signalling and/or one or more of ascorbic acid signalling activators and/or additional factors. Methods and kits for maintaining LB in self renewal state, hepatocyte-like cells in perivenous or periportal state, as well as surface markers for LB and mid/hindgut (MHG) cells are also disclosed.
摘要翻译：本公开提供了用于将干细胞分化为相关Iiver细胞谱系的方法和试剂盒，以及使用相关Iiver细胞谱系筛选细胞应答，表型和治疗病症的方法。在一个实施方案中，首先将干细胞分化成定形内胚层谱系的细胞，其通过一种或多种视黄酸活化剂和/或一种或多种转化生长因子-β抑制剂分化成后部前肠（PFG）谱系细胞 TGFβ）。另一个实施方案提供了通过TGFβ信号传导的一种或多种激活剂和/或一种或多种Wnt信号传导调节剂和/或一种或多种环化激活剂将后部前肠系细胞分化成伊布尔芽祖细胞（LB） AMP / PKA信号传导; 并且进一步的实施方案提供了通过TGFβ信号传导和/或成纤维细胞生长因子（FGF）抑制剂和/或一种或多种Notch抑制剂的一种或多种抑制剂将Iber芽祖细胞分化成肝祖细胞的方法。另一实施方案公开了通过一种或多种Notch抑制剂和/或糖皮质激素信号传导激活剂和/或一种或多种胰岛素信号传导激活剂和/或一种或多种下列物质将肝祖细胞分化成肝细胞样细胞或肝细胞样细胞的方法：抗坏血酸信号传导活化剂和/或其他因子。还公开了用于维持LB自我更新状态的方法和试剂盒，静脉或门静脉周期状态的肝细胞样细胞以及LB和中/后肠（MHG）细胞的表面标记。

82. 发明公开

EP3200843A1 USE OF REGENERATIVE CELLS IN MITIGATING BURN PROGRESSION AND IMPROVING SKIN GRAFT INCORPORATION AND HEALING 审中-公开
标题翻译：再生细胞在减缓烧伤进展和改善皮肤移植结合和愈合中的应用
公开(公告)号：EP3200843A1
公开(公告)日：2017-08-09
申请号：EP15847625.9
申请日：2015-10-02
申请人： Cytori Therapeutics, Inc.
发明人： FRASER, John, K. , FOUBERT, Philippe , ALFONSO, Zeni
IPC分类号： A61L27/60
CPC分类号： A61L27/24 , A61L27/00 , A61L27/3834 , A61L27/56 , A61L27/60 , C12N5/00 , C12N5/0653 , C12N5/0667
摘要： Described herein are compositions and methods for the mitigation of burn progression. In particular, the described herein are compositions including regenerative cells for use in preventing and reducing burn progression. Also described are compositions and methods for improving graft take and healing, and preventing and/or treating hypertrophic scars.
摘要翻译：本文描述了用于缓解燃烧进展的组合物和方法。特别地，本文所述的组合物包括用于预防和减少烧伤进展的再生细胞。还描述了用于改善移植物采取和愈合，以及预防和/或治疗肥厚性瘢痕的组合物和方法。

83. 发明公开

EP3184627A1 CULTURE MEDIUM FOR MESENCHYMAL STEM CELLS 有权转让
标题翻译： MESENCHYMAL STEM细胞的培养基
公开(公告)号：EP3184627A1
公开(公告)日：2017-06-28
申请号：EP15833662.8
申请日：2015-08-20
申请人： Ajinomoto Co., Inc. , Kyoto University
发明人： JIN, Yonghui , HARATA, Ikue , SENDA, Sho , OZAWA, Hiroki , KOBAYASHI, Tsuyoshi , TOGUCHIDA, Junya , IKEYA, Makoto
IPC分类号： C12N5/00 , A61K35/55 , A61K35/56 , A61P19/00 , A61P19/02 , A61P19/04 , A61P43/00 , C12N5/0775
CPC分类号： C12N5/0663 , A61K35/28 , A61K35/55 , A61K35/56 , A61K2035/124 , C12N5/00 , C12N2500/32 , C12N2500/84
摘要： The problem of the present invention is to provide a means that enables conventionally-difficult growth and culture of mesenchymal stem cells for a long period, without using any special apparatus, equipment and the like. The above problem is solved by providing a medium for mesenchymal stem cells in which seven kinds of nonessential amino acids of glycine, alanine, serine, proline, asparagine, aspartic acid and glutamic acid are reduced.
摘要翻译：本发明的课题在于提供一种不使用特别的装置，设备等，能够长期进行常规难以间充质干细胞的生长培养的手段。通过提供其中甘氨酸，丙氨酸，丝氨酸，脯氨酸，天冬酰胺，天冬氨酸和谷氨酸的七种非必需氨基酸被还原的间充质干细胞培养基来解决上述问题。

84. 发明授权

EP2808341B1 Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells 有权转让
标题翻译：使用分子伴侣在哺乳动物细胞中增强产生分泌的重组蛋白质
公开(公告)号：EP2808341B1
公开(公告)日：2017-04-26
申请号：EP14162830.5
申请日：2004-03-16
申请人： Bayer HealthCare LLC
发明人： Chan, Sham-Yuen , Tang, Hsinyi, Yvette , Tao, Yiwen , Kelly, Ruth , Wu, Yongjian
IPC分类号： C07K14/755 , C12N15/85 , C07K14/81 , C12P21/00 , C12N5/00
CPC分类号： C12P21/02 , C07K14/435 , C07K14/755 , C07K14/8114 , C12N5/00 , C12N15/85 , C12N2501/07 , C12N2501/998 , C12N2510/02 , C12P21/00

85. 发明公开

EP3021119A4 METHOD FOR DIAGNOSIS OF DISEASES USING MORPHOLOGICAL CHARACTERISTICS OF LUTERIAL 审中-公开
标题翻译：方法的疾病诊断中的应用LUTERIAL的形态特征
公开(公告)号：EP3021119A4
公开(公告)日：2017-04-19
申请号：EP14823421
申请日：2014-01-14
申请人： KWON YOUNG AH , CHOI WON CHEOL , CHOI SUK HOON , CHOI CHANG HOON
发明人： KWON YOUNG AH , CHOI WON CHEOL , CHOI SUK HOON , CHOI CHANG HOON
IPC分类号： G01N33/53 , A61K35/12 , C12N5/07 , G01N33/48 , G01N33/58
CPC分类号： G01N33/5091 , A61K35/00 , C12N5/00 , G01N2800/50 , G01N2800/52 , G01N2800/7028

86. 发明公开

EP3138903A1 CELLS FOR PRODUCING HUMAN ANTIBODY 有权转让
标题翻译： ZELLEN ZUR HERSTELLUNG EINES HUMANENANTIKÖRPERS
公开(公告)号：EP3138903A1
公开(公告)日：2017-03-08
申请号：EP15786546.0
申请日：2015-05-01
申请人： Chiome Bioscience, Inc.
发明人： HASHIMOTO, Shuichi , UCHIKI, Tomoaki , KAWATA, Shigehisa , ASAGOSHI, Kenjiro , YABUKI, Takashi , SANO, Hitomi , MIYAI, Shunsuke , TAKAHASHI, Naoki , TAKESUE, Aki , SAWADA, Atsushi
IPC分类号： C12N5/00 , C07K16/18 , C07K16/22 , C12N5/10 , C12N15/00 , C12N15/09 , C40B40/02
CPC分类号： C07K16/28 , C07K16/18 , C07K16/22 , C07K16/244 , C07K2317/14 , C07K2317/21 , C07K2317/51 , C07K2317/515 , C07K2317/565 , C12N5/00 , C12N5/0635 , C12N5/10 , C12N15/00 , C12N15/09 , C12N2510/02 , C40B40/02
摘要： It is an object of the present invention to provide a chicken B cell that expresses a variety of human antibodies. The solution to the problem in the present invention is a chicken B cell, in which, in an antibody light chain gene locus thereof, all or a part of a DNA sequence derived from a human antibody light chain variable region and a human antibody light chain constant region are inserted, or the antibody light chain gene locus is replaced with all or a part of a DNA sequence derived from a human antibody light chain variable region and a human antibody light chain constant region, and in an antibody heavy chain gene locus thereof, all or a part of a DNA sequence derived from a human antibody heavy chain variable region and a human antibody heavy chain constant region are inserted, or the antibody heavy chain gene locus is replaced with all or a part of a DNA sequence derived from a human antibody heavy chain variable region and a human antibody heavy chain constant region, and in an antibody light chain pseudogene locus thereof, two or more DNA sequences derived from human antibody light chain variable regions are inserted, or the antibody light chain pseudogene locus is replaced with two or more DNA sequences derived from human antibody light chain variable regions, and/or in an antibody heavy chain pseudogene locus thereof, two or more DNA sequences derived from human antibody heavy chain variable regions are inserted, or the antibody heavy chain pseudogene locus is replaced with two or more DNA sequences derived from human antibody heavy chain variable regions.
摘要翻译：本发明的目的是提供一种表达多种人抗体的鸡B细胞。本发明的问题的解决方案是一种鸡B细胞，其中在抗体轻链基因座中，全部或部分源自人抗体轻链可变区和人抗体轻链的DNA序列或者抗体轻链基因座被全部或一部分来自人抗体轻链可变区和人抗体轻链恒定区的DNA序列的全部或部分置换，并且其抗体重链基因座插入来自人抗体重链可变区和人抗体重链恒定区的DNA序列的全部或一部分，或者将全部或部分来源于人抗体重链可变区和人抗体重链恒定区，并且在其抗体轻链假基因座中，衍生自人抗体轻链的两个或更多个DNA序列插入可变区，或者用两条或更多种源自人抗体轻链可变区的DNA序列和/或其抗体重链假基因座取代抗体轻链假基因座，得自两个或更多个源自人的DNA序列插入抗体重链可变区，或用来自人抗体重链可变区的两个或多个DNA序列替换抗体重链假基因座。

87. 发明公开

EP3137595A2 NOVEL VERTEBRATE CELLS AND METHODS FOR RECOMBINANTLY EXPRESSING A POLYPEPTIDE OF INTEREST 有权
标题翻译：新型涡动物细胞和关心的多肽的重组表达方法
公开(公告)号：EP3137595A2
公开(公告)日：2017-03-08
申请号：EP15739327.3
申请日：2015-04-29
申请人： Novartis AG
发明人： LAUX, Holger , ROMAND, Sandrine , BODENDORF, Ursula
IPC分类号： C12N5/00 , C12N5/071 , C12N15/67 , C12N15/87 , C12N15/85
CPC分类号： C12P21/00 , C12N5/00 , C12N9/6424 , C12N15/907 , C12N2510/02 , C12Y304/21109 , G01N15/14 , G01N2015/0065 , G01N2015/1006
摘要： The present application pertains inter alia to an isolated vertebrate cell suitable for recombinant expression of a polypeptide of interest, wherein the vertebrate cell is altered to impair the function of the endogenous protease matriptase and wherein said cell comprises at least one heterologous polynucleotide encoding a polypeptide of interest and wherein the polypeptide of interest is secreted by the cell. It was found that using respective vertebrate cells for producing a recombinant polypeptide of interest significantly reduces clipping of the polypeptide of interest that is secreted into the cell culture medium. Also provided are improved production and screening methods.
摘要翻译：本申请涉及尤其对适合于所关注的多肽的重组表达分离的脊椎动物细胞，worin脊椎动物细胞被改变，损害内源性蛋白酶蛋白裂解酶和worin的函数的所述细胞包含编码的多肽的至少一种异源多核苷酸兴趣和worin感兴趣的多肽是由该细胞分泌。结果发现使用respectivement脊椎动物细胞生产感兴趣的重组多肽也极大减少到细胞培养基中没有的感兴趣的多肽的限幅被分泌。所以，提供的是提高了生产和筛选方法。

88. 发明公开

EP3075844A1 A METHOD OF DETERMINING A COMPOSITIONAL OR FUNCTIONAL CHARACTERISTIC OF A CELL CULTURE MEDIA 审中-公开
标题翻译：方法确定化合物或功能状态的细胞培养基
公开(公告)号：EP3075844A1
公开(公告)日：2016-10-05
申请号：EP15162281.8
申请日：2015-04-01
申请人： Valitacell Limited
发明人： THOMPSON, Ben , JAMES, David , CLIFFORD, Jerry
IPC分类号： C12N5/00
CPC分类号： C12N5/00 , G01N33/502
摘要： A method of determining or predicting a characteristic of a query cell culture media comprises the steps of (a) generating an environmental response fingerprint specific for query cell culture media according to a method of any of Claims 1 to 3, (b) comparing the query cell culture media-specific environmental response fingerprint of step (a) with a reference media-specific environmental response fingerprints corresponding to a reference media having a known characteristics and generated using the reference cell, and (c) determining or predicting a characteristic of the query cell culture media based on the level of correlation between the query cell culture media-specific environmental response fingerprint and the reference media-specific environmental response fingerprints.
摘要翻译：确定性采矿或预测的查询的细胞培养基的特性的方法包括（a）在特定的查询细胞培养基雅丁环境响应指纹的任何权利要求1至3中的方法来生成，（b）比较所述查询的步骤步骤的特定媒介的细胞培养环境的响应指纹（a）用参考特定媒体环境响应指纹对应于参考媒体具有已知特性和生成使用所述参考单元，以及（c）确定的采矿或预测的查询的特征基于查询的细胞培养特定媒介的环境响应指纹和参照特定的媒体环境响应指纹之间的相关性的电平的细胞培养基。

89. 发明公开

EP3055409A1 METABOLICALLY OPTIMIZED CELL CULTURE 有权
标题翻译：代谢优化细胞培养
公开(公告)号：EP3055409A1
公开(公告)日：2016-08-17
申请号：EP14851841.8
申请日：2014-10-10
申请人： Regeneron Pharmaceuticals, Inc.
发明人： LAWRENCE, Shawn , KIM, Ann , JOHNSON, Amy
IPC分类号： C12N5/02 , C12N5/16
CPC分类号： C12N5/0682 , C07K16/00 , C07K2317/14 , C12N5/00 , C12N2500/60 , C12N2511/00 , C12P21/02
摘要： An improved method for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

90. 发明公开

EP3050957A1 AUTOMATIC CULTURE SYSTEM AND AUTOMATIC CULTURE DEVICE 有权转让
标题翻译：自动化系统自动化系统
公开(公告)号：EP3050957A1
公开(公告)日：2016-08-03
申请号：EP14848746.5
申请日：2014-09-19
申请人： TOKYO ELECTRON LIMITED
发明人： OZAKI, Shigenori , FUJI, Toshimitsu , KINOSHITA, Yoshio , TAMURA, Akitake
IPC分类号： C12M1/00 , C12N1/00
CPC分类号： C12M41/48 , C12M23/44 , C12M23/50 , C12M23/52 , C12M37/00 , C12M37/04 , C12M41/14 , C12N5/00 , C12N5/0068
摘要： An automatic culture system includes a transfer part configured to transfer a first airtight container which accommodates cells in a sealed state, and an automatic culture device configured to receive the first airtight container transferred by the transfer part, take out the cells from the first airtight container and culture the taken-out cells. The automatic culture device is configured to automatically perform replacement of a culture medium.
摘要翻译：一种自动培养系统，其特征在于，包括：转印部，其配置成将密闭容纳细胞的第一气密容器转印;以及自动培养装置，其被配置为接收由所述转印部转印的第一气密容器，从所述第一气密容器并培养取出的细胞。自动培养装置被配置为自动执行培养基的更换。

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