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1. 发明公开

EP4375364A2 POLLEN FIELD CONDITIONING AND PRESERVATION METHOD 审中-公开
公开(公告)号：EP4375364A2
公开(公告)日：2024-05-29
申请号：EP24169438.9
申请日：2017-04-13
申请人： Accelerated AG Technologies, LLC
发明人： Etter, Sara Katherine , Cope, Jason , Singletary, George , Krone, Todd
IPC分类号： C12M3/00
CPC分类号： A01H1/02 , C12M3/00
摘要： Described are methods of preserving pollen, which may be subsequently stored. A method of the present invention includes collecting fresh pollen and introducing the pollen to a controlled environment which regulates pollen moisture content. The controlled environment may have a temperature ranging from about -10-10 °C; an adjustable and programmable relative humidity; a continuous, adjustable positive or negative air flow; and a flow of one or more continuously refreshed, selected gases which displace oxygen. In another embodiment of the method, the controlled environment may have a relative humidity from about 50-100%; a temperature from about -10-10 °C; and air pressure from about 15-150 kPa. In all embodiments of the method, an optional field conditioning step may be performed. The field conditioning step may include subjecting the pollen to an environment controlled for relative humidity, temperature, and air pressure such that the initial pollen moisture content may be adjusted to a target moisture content.

2. 发明公开

EP4375359A1 CULTURE SYSTEM 审中-公开
公开(公告)号：EP4375359A1
公开(公告)日：2024-05-29
申请号：EP22845979.8
申请日：2022-07-19
申请人： SAGA UNIVERSITY
发明人： NAKAYAMA, Koichi , NAKAMURA, Anna Maria
IPC分类号： C12M1/00 , C12M3/00 , C12N5/077 , C12N5/0775
CPC分类号： C12N5/06 , C12M1/00 , C12M3/00
摘要： A culture system that comprises: a support for stacking cell aggregates, said support being provided with needle-shaped parts that are arranged on a substrate like a kenzan (flower frog needles), in which the outermost needle-shaped parts on the substrate are arranged so that the distance from the needle-shaped parts to the outer edge of the substrate is shorter than the distance between adjacent needle-shaped parts; a culture tank for housing a culture unit in which cell aggregates are stacked on the support; a liquid culture medium container; and a plurality of liquid culture medium supply pipes that supply a liquid culture medium from the liquid culture medium container to the culture unit, wherein each of the plurality of liquid culture medium supply pipes is positioned between the needle-shaped part.

3. 发明公开

EP4361255A1 CELL CULTURE METHOD, CELL CULTURE KIT, AND CELL CULTURE SYSTEM 审中-公开
公开(公告)号：EP4361255A1
公开(公告)日：2024-05-01
申请号：EP22828012.9
申请日：2022-03-26
申请人： Toyo Seikan Group Holdings, Ltd.
发明人： NISHIYAMA, Takaharu , TANAKA, Satoshi , KOSEKI, Osamu , TOTANI, Takahiko
IPC分类号： C12N5/071 , C12M3/00 , C12N5/07 , C12N5/10
CPC分类号： C12N5/06 , C12M3/00 , C12N5/10
摘要： Provided is a cell culture method that can suitably control the exchange rate of a medium inside a vessel in the cell culture using a culture vessel. The cell culture method uses a culture vessel and a medium supplying vessel. Cells and a medium are filled and cell culture is performed in the culture vessel, a new medium is filled in the medium supplying vessel, and the medium supplying vessel supplies the medium to the culture vessel. The culture vessel includes at least a first port used for medium supply and a second port used for medium discharge, and the medium supplying vessel serves as a mixing vessel for adjusting a concentration of the new medium to be supplied to the culture vessel. The cell culture method includes: transferring a portion of a used medium in the culture vessel from the culture vessel to the mixing vessel; creating a concentration-adjusted medium by mixing the new medium and the portion of the used medium in the mixing vessel; and filling the culture vessel with the concentration-adjusted medium by discharging a remaining used medium from the culture vessel while the concentration-adjusted medium is transferred from the mixing vessel to the culture vessel.

4. 发明公开

EP3354719A1 SUBSTRATE FOR CELL CULTURE, CELL CULTURE METHOD USING SAME, CELL CULTURE VESSEL, AND USE AS SUBSTRATE 审中-公开
公开(公告)号：EP3354719A1
公开(公告)日：2018-08-01
申请号：EP16848700.7
申请日：2016-09-23
申请人： Mitsubishi Gas Chemical Company, Inc.
发明人： ANDO, Haruna , IIDA, Shin , YAMAGUCHI, Yasunori
IPC分类号： C12M3/00 , C08G63/199 , C08G63/66 , C08L67/02 , C12N5/07
CPC分类号： C12N5/0656 , C08G63/199 , C08G63/66 , C08L67/02 , C12M3/00 , C12N2533/30
摘要： The present invention provides a base material for cell culture comprising a polyester resin comprising a dicarboxylic acid unit and a diol unit, wherein 1 to 100% by mol of the diol units is a diol unit derived from 1,4-cyclohexanedimethanol.

5. 发明公开

EP3351627A1 PLATELET PRODUCTION METHOD USING ROTARY AGITATION CULTURING METHOD 审中-公开
公开(公告)号：EP3351627A1
公开(公告)日：2018-07-25
申请号：EP16846363.6
申请日：2016-09-08
申请人： Megakaryon Corporation
发明人： SHIGEMORI, Tomohiro , OKAMOTO, Haruki
IPC分类号： C12N5/078 , A61K35/14 , A61K35/19 , C12M3/00
CPC分类号： C12N5/0644 , A61K35/14 , A61K35/19 , C12M3/00 , C12N2506/13 , C12N2527/00
摘要： The present invention provides a method for producing platelets, and the method comprises a step of culturing megakaryocytes in a culture solution in a culture vessel, wherein the culture solution is stirred with a stirrer in the culture step.

6. 发明公开

EP3202896A4 METHOD FOR CULTURING PLURIPOTENT STEM CELLS 审中-公开
公开(公告)号：EP3202896A4
公开(公告)日：2018-07-04
申请号：EP15845721
申请日：2015-09-30
申请人： JTEC CORP
发明人： UEMURA TOSHIMASA , ONOMURA YUI , TSUMURA TAKASHI
IPC分类号： C12N5/10 , C12N5/0735 , C12N5/074
CPC分类号： C12N5/0696 , C12M3/00 , C12M37/02 , C12M41/40 , C12N5/10 , C12N2501/727 , C12N2525/00 , C12N2531/00 , C12N2539/00
摘要： Provided is a method for efficiently culturing pluripotent stem cells with higher safety. The present invention relates to a method for culturing pluripotent stem cells, the method comprising culturing an isolated pluripotent stem cells in a pseudo-microgravity environment to proliferate the pluripotent stem cells while maintaining the pluripotent stem cells in an undifferentiated state, thereby forming and growing spheroids of the pluripotent stem cells; and a method for inducing differentiation of pluripotent stem cells by using the method.

7. 发明公开

EP3315509A1 POLYMERIZED PEPTIDE AND GEL HAVING COLLAGEN-LIKE STRUCTURE 有权转让
标题翻译：具有胶原样结构的聚合肽和凝胶
公开(公告)号：EP3315509A1
公开(公告)日：2018-05-02
申请号：EP16814442.6
申请日：2016-06-23
申请人： Waseda University , Kola-Gen Pharma Inc.
发明人： KOIDE Takaki , ICHISE Shinichiro , TAKEUCHI Shungo , NOSE Hiroshi
IPC分类号： C07K14/00 , A61K38/00 , A61L15/00 , A61P17/02 , C07K1/02 , C07K14/78 , C12M3/00
CPC分类号： C07K14/78 , A61K38/00 , A61L15/00 , A61L15/325 , C07K1/02 , C07K14/00 , C08J3/075 , C12M3/00 , C12N5/0018
摘要： Although collagen is useful as a medical material, there exist risks of allergies and zoonoses when used in human. There, artificial collagens or the like are being considered as medical materials. However, the conventional artificial collagens were not necessarily satisfactory in terms of, for example, physical strength, denaturation due to heating, and inability of being processed into the shape of a sheet i.e. the conventional artificial collagens have been problematic in terms of practicality. The present invention is produced as a polymerized peptide prepared by oxidatively cross-linking collagen-like peptides having the triple-helical structure containing three peptide chains each containing multiple cysteine (Cys) residues. This polymerized peptide can be processed into a hydrogel and a thin membrane sheet before use, and is capable of imparting a particular physiological function such as cellular adhesion by incorporating functional amino acid sequences present on biological polymers. Further, the present invention provides a method for producing the abovementioned polymerized peptide and the like; and use applications employing the same, such as cell culturing, wound dressing, and compositions including regenerative medical materials and research materials.
摘要翻译：虽然胶原蛋白作为医疗材料是有用的，但在人体中使用时存在过敏和人畜共患病的风险。在那里，人造胶原蛋白等被认为是医学材料。然而，就例如物理强度，由于加热引起的变性以及不能被加工成片状而言，常规人造胶原不一定令人满意，即，常规人造胶原在实用性方面存在问题。本发明作为通过氧化交联具有含有各自含有多个半胱氨酸（Cys）残基的三条肽链的三螺旋结构的胶原样肽而制备的聚合肽来制备。该聚合肽可以在使用前加工成水凝胶和薄膜片，并且能够通过掺入存在于生物聚合物上的功能性氨基酸序列赋予特定的生理功能，如细胞粘附。此外，本发明提供了生产上述聚合肽等的方法; 并使用使用其的应用，例如细胞培养，伤口敷料和包括再生医学材料和研究材料的组合物。

8. 发明公开

EP3196293A4 THREE-DIMENSIONAL CELL CULTURE SYSTEM AND CELL CULTURE METHOD USING SAME 审中-实审
公开(公告)号：EP3196293A4
公开(公告)日：2018-04-25
申请号：EP15843006
申请日：2015-09-15
申请人： CEFO CO LTD
发明人： PARK HYUN SOOK , LEE SUN RAY , YANG JI WON , CHU SEOL , PARK JANG MI , MO HYUN JUNG
IPC分类号： C12N5/0775 , A01N1/02 , A61K35/12 , A61L27/38 , A61L27/60 , C12M1/12 , C12N5/00
CPC分类号： C12N5/0062 , A01N1/02 , A61K35/12 , A61L27/38 , A61L27/60 , C12M3/00 , C12M25/14 , C12N5/00
摘要： The present invention relates to a composition for preserving cells and a cell preservation method and, more specifically: to a composition for preserving cells, containing, as active ingredients, a plant-derived recombinant human serum albumin and a plant peptide, which maintain a high cell survival rate while maintaining animal-free and xeno-free properties and are stable without changes in cell morphology or a surface expression factor during the short term preservation of cells such as stem cells or primary cultured cells; and a cell preservation method using the same.

9. 发明公开

EP3213776A4 TUBULAR STRUCTURE, DEVICE FOR PRODUCING TUBULAR STRUCTURE, AND METHOD FOR PRODUCING TUBULAR STRUCTURE 有权转让
公开(公告)号：EP3213776A4
公开(公告)日：2017-11-22
申请号：EP15855417
申请日：2015-10-30
申请人： FUJIFILM CORP
发明人： NAKAMURA KENTARO
IPC分类号： A61L27/00 , A61L27/22 , A61L27/38 , A61L27/50 , A61L27/56 , A61L27/58 , C12M3/00
CPC分类号： A61L27/507 , A61F2/06 , A61L27/22 , A61L27/222 , A61L27/225 , A61L27/227 , A61L27/3604 , A61L27/38 , A61L27/3804 , A61L27/54 , A61L27/56 , A61L27/58 , A61L2300/80 , C12M3/00 , C12N5/0068
摘要： An object of the present invention is to provide a cell-containing bioabsorbable tubular structure having molecular permeability, a device for manufacturing the tubular structure, and a method for manufacturing the tubular structure. According to the present invention, there is provided a tubular structure constituted with a cell structure which contains biocompatible polymer blocks and cells, in which the plurality of polymer blocks is disposed in voids between the plurality of cells.

10. 发明公开

EP3214165A1 FIBER STRUCTURE FOR USE AS CELL SCAFFOLD MATERIAL 审中-公开
标题翻译：用作电池支架材料的纤维结构
公开(公告)号：EP3214165A1
公开(公告)日：2017-09-06
申请号：EP15855508.6
申请日：2015-10-30
申请人： Toray Industries, Inc.
发明人： KADOWAKI, Koji , KUGA, Chisa , FUJITA, Masaki , TANAHASHI, Kazuhiro , YAMADA, Satoshi , KANEKO, Takayuki , TSUCHIKURA, Hiroshi
IPC分类号： C12M3/00 , A61L27/00 , D01F6/62 , D03D1/00
CPC分类号： C12N5/0068 , A61L27/18 , A61L27/50 , A61L27/56 , C12M3/00 , C12N2533/30 , D01F6/62 , D03D1/00 , D03D15/0088 , C08L67/02
摘要： The present invention aims to provide a fiber structure which can be used as a cell scaffold material showing improvement in both the cellular adhesiveness and the cell growth capacity, which improvement was achieved by controlling the fiber orientation in the multifilament and the average fiber diameter, which are physical properties. The present invention provides a fiber structure which can be used as a cell scaffold material, which fiber structure comprises a multifilament formed by bundling monofilaments having an average fiber diameter of 1 to 15 µm, wherein each of the monofilaments satisfies the condition of the following Formula 1: (Y/X)×100≥50 ... Formula 1 [wherein in Formula 1, X represents the number of monofilaments for which the average crossing angle is investigated, and Y represents the number of monofilaments having an average crossing angle of not more than 25° in X].
摘要翻译：本发明的目的在于提供一种纤维结构体，该纤维结构体能够作为通过控制复丝中的纤维取向度和平均纤维径而实现的提高细胞粘附性和细胞生长能力的细胞支架材料，是物理性质。本发明提供可用作细胞支架材料的纤维结构体，所述纤维结构体包含通过捆束平均纤维直径为1至15μm的单丝而形成的复丝，其中每根单丝满足下式 1：（Y / X）×100≥50...式1 [式1中，X表示调查平均交叉角度的单丝的数量，Y表示平均交叉角度为 X中不超过25°]。

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