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    • 86. 发明公开
    • DEVICE FOR ANALYZING SINGLE CELL USING MICROPORES
    • EP4067865A1
    • 2022-10-05
    • EP22164244.0
    • 2022-03-24
    • Korea University Research and Business Foundation
    • HAN, Chang Soo
    • G01N15/12
    • Disclosed is a device for analyzing a single cell using a micropore (310), including an inlet chamber (100) in which a solution including a target cell (C) is contained; an outlet chamber (200) provided on an opposite side of the inlet chamber; a pore membrane (300) disposed between the inlet chamber and the outlet chamber and including a micropore (310) therein; a pressure generating means (400) provided in the inlet chamber or the outlet chamber and configured to generate a pressure difference between the inlet chamber and the outlet chamber such that a solution in the inlet chamber flows toward the outlet chamber to pass through the micropore; and a pair of electrodes (510, 520) respectively disposed in front and rear of the pore membrane and provided to be in electrical contact with the flowing solution to measure an ion conductivity change in front and rear of the pore membrane, wherein a diameter D of the micropore (310) is larger than a diameter of the target cell (C), a thickness t of the pore membrane (300) is 0.5 µm to 1mm, and a slenderness ratio (t/D) is 0.001 to 5.
      According to the present disclosure, a deformed cell can be rapidly and accurately detected by measuring the deformed characteristic of single cell by shear force, and the number, size, and deformability of the cell when the cell passes through a pore with a micro-sized diameter in a flow field generated by a pressure difference.