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61. 发明公开

EP1399588A2 METHOD AND SEQUENCES FOR DETERMINATE NUCLEIC ACID HYBRIDIZATION 有权
标题翻译：方法和序列确定性核酸杂交
公开(公告)号：EP1399588A2
公开(公告)日：2004-03-24
申请号：EP02723698.3
申请日：2002-03-27
申请人： Applied Minds, Inc.
发明人： HILLIS, William, Daniel
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6832 , C12Q1/6874 , C12Q2525/101 , C12Q2525/117
摘要： Provided are methods for using nucleic acid sequences having two or more degenerately pairing nucleotides, each degenerate nucleotide having a partially overlapping set of complementarity, to reduce the number of hybridizing nucleotide sequences or probes used in biochemical and molecular biological operations having sequence specific hybridization. The method may be employed for various hybridization procedures with sequence specific hybridization, including sequencing methods measuring hybridization directly, and tagging by hybridization methods in which the sequence is determined by analyzing the pattern of tags that hybridize thereto, and hybridization dependent amplification methods. The method involves hybridizing to the nucleic acid sequence of interest a first hybrizing nucleotide sequence and a second hybridizing nucleotide sequence, each comprising a sequence complementary, or complementary except at a position of interest or variable position, to a nucleic acid sequence of interest, and analyzing the whether some, all or none of the probes or tags hybridize.

62. 发明公开

EP1363640A2 NUCLEIC ACID DERIVATIVES 有权
标题翻译：核酸衍生物
公开(公告)号：EP1363640A2
公开(公告)日：2003-11-26
申请号：EP02711178.0
申请日：2002-01-29
申请人： BIO-RAD LABORATORIES, INC.
发明人： SEGEV, David
IPC分类号： A61K31/535 , A61K31/505 , A61K31/22 , A61K31/045 , C07C31/18 , C07C31/24 , C07C67/02 , C07C69/74 , C07D239/02 , C07D265/34 , C07D267/22 , C07D403/00
CPC分类号： C12Q1/6839 , C07B2200/11 , C12Q1/6832 , C12Q2525/117 , C12Q2525/113
摘要： A compound which comprises a backbone having a plurality of chiral carbon atoms, the backbone bearing a plurality of ligands each being individually bound to a chiral carbon atom of the plurality of chiral carbon atoms, the ligands including one or more pair(s) of adjacent ligands each containing a moiety selected from the group consisting of a naturally occurring nucleobase and a nucleobase binding group, wherein moieties of the one or more pair(s) are directly linked to one another via a linker chain; building blocks for synthesizing the compound; and uses of the compound, particularly in antisense therapy.
摘要翻译：包含具有多个手性碳原子的骨架的化合物，所述骨架带有多个配体，每个配体各自独立地与多个手性碳原子的手性碳原子结合，所述配体包括一个或多个相邻的各自含有选自天然存在的核碱基和核碱基结合基团的部分的配体，其中所述一个或多个对的部分通过接头链彼此直接连接; 用于合成该化合物的结构单元; 和该化合物的用途，特别是在反义治疗中。

63. 发明公开

EP1323833A1 METHOD OF DETERMINING BASE SEQUENCE 审中-公开
标题翻译： VERFAHREN ZUR BESTIMMUNG EINER BASENSEQUENZ
公开(公告)号：EP1323833A1
公开(公告)日：2003-07-02
申请号：EP01963416.1
申请日：2001-09-05
申请人： Ajinomoto Co., Inc.
发明人： KIMURA, Eiichiro
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6823 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101 , C12Q2525/117
摘要： A nucleotide sequence of a single nucleotide or plural nucleotides can be determined by assaying reactions in one reaction solution.
By detecting a reaction product continuously generated in an extension reaction system following the extension reaction of polynucleotide chain and thereby determining the alignment in the sequentially extending polynucleotide, the nucleotide sequence can be determined.
摘要翻译：可以通过测定一种反应溶液中的反应来确定单个核苷酸或多个核苷酸的核苷酸序列。通过检测在多核苷酸链的延伸反应之后在延伸反应体系中连续产生的反应产物，从而确定顺序延伸的多核苷酸中的比对，可以确定核苷酸序列。

64. 发明授权

EP0628051B1 APPLICATIONS OF FLUORESCENT N-NUCLEOSIDES AND FLUORESCENT STRUCTURAL ANALOGS OF N-NUCLEOSIDES 无效
标题翻译：荧光的N-核苷和其模拟的使用
公开(公告)号：EP0628051B1
公开(公告)日：2003-07-02
申请号：EP93905954.9
申请日：1993-02-12
申请人： Chromagen, Inc.
发明人： CONRAD, Michael, J.
IPC分类号： C07H21/00 , C12Q1/68 , C07F9/6561 , C07H19/04 , C07H19/10 , C07H19/20
CPC分类号： C07H21/00 , C07H19/04 , C12Q1/6832 , C12Q2525/117
摘要： Structural analogs of the six non-fluorescent N-nucleosides commonly found in RNA and DNA, which are inherently fluorescent under physiological conditions, are identified and methods for their preparation provided. Such analogs may be incorporated into DNA and/or RNA oligonucleotides via either enzymatic or chemical synthesis to produce fluorescent oligonucleotides having prescribed sequences. Such analogous sequences may be identical to, or the analogous complement of, template or target DNA or RNA sequences to which the fluorescent oligonucleotides can be hybridized. Methods of preparing either RNA or DNA oligonucleotide probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in oligonucleotide amplification, detection, identification, and/or hybridization assays are also provided.

65. 发明公开

EP1296997A1 BASE ANALOGUES 有权
标题翻译：碱基类似物
公开(公告)号：EP1296997A1
公开(公告)日：2003-04-02
申请号：EP01947617.5
申请日：2001-06-29
申请人： Amersham Biosciences UK Limited
发明人： KUMAR, Shiv,Amersham Pharmacia Biotech , MCDOUGALL, Mark,Amersham Pharmacia Biotech , NAMPALLI, Satyam,Amersham Pharmacia Biotech , NEAGU, Constantin,Amersham Pharmacia Biotech , LOAKES, David , BROWN, Dan
IPC分类号： C07H19/23 , C07D487/04 , C07F9/6561 , C07H21/00 , C12Q1/68 , G01N33/53 , A61K31/706 , A61P31/12
CPC分类号： C07D487/04 , C07F9/6561 , C07H19/23 , C12Q2525/117 , G01N33/58
摘要： The present invention describes novel compounds of the formula (I) wherein Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue, Y=O, S, NR10, where R10 is H, alkyl alkenyl, alkynyl, X is H, alkyl, alkenyl, alkynyl, aryl, heteroaryl or a combination thereof or, preferably, a reporter group. The novel compounds are suitable for incorporation in oligonucleotides and polynucleotides.

66. 发明公开

EP1225234A2 Synthesis of nucleic acid 有权
标题翻译： Verfahren zur Sequenzierung vonNukleinsäuren
公开(公告)号：EP1225234A2
公开(公告)日：2002-07-24
申请号：EP02250379.1
申请日：2002-01-21
申请人： Agilent Technologies, Inc. (a Delaware corporation)
发明人： Sampson, Jeffrey R.
IPC分类号： C12Q1/68
CPC分类号： G01N33/48721 , C12Q1/6806 , C12Q1/6869 , C12Q2565/631 , C12Q2525/117 , C12Q2525/101 , C12Q2525/143 , C12Q2531/125
摘要： A nucleic acid molecule to be sequenced is generated having tandem repeats of a sequence, and also having modified nucleotides which reduce the levels of secondary structure. The presence of tandemly repeated sequence and the absence of secondary structure increases the rate of sequencing and accuracy of sequences generated by nanopore sequencing. A method comprises the steps of: providing two separate, adjacent solutions of a medium and an interface between the two pools, the interface having a channel so dimensioned as to allow sequential nucleotide-by-nucleotide passage from one pool to the other pool of only one nucleic acid molecule at a time; providing a nucleic acid molecule with at least one repeat of a nucleotide sequence to be determined, wherein the nucleic acid molecule is enzymatically synthesized using a circular template, and wherein the nucleic acid molecule contains modified nucleotides that reduce secondary structure in the nucleic acid molecule; placing the nucleic acid molecule in one of the two pools; and taking measurements as each of the nucleotides of the nucleic acid molecule passes through the channel so as to determine the sequence of the nucleic acid molecule.
摘要翻译：产生待测序的核酸分子，其具有序列的串联重复序列，并且还具有降低二级结构水平的经修饰的核苷酸。串联重复序列的存在和不存在二级结构增加了通过纳米孔测序产生的序列的测序速率和准确性。一种方法包括以下步骤：提供在两个池之间的介质和界面的两个独立的相邻溶液，所述界面具有如此尺寸的通道，以允许从一个池到另一个池的顺序核苷酸逐个核苷酸通过一个核酸分子; 向核酸分子提供待测定的核苷酸序列的至少一个重复序列，其中所述核酸分子使用圆形模板酶促合成，并且其中所述核酸分子含有修饰的核苷酸，其降低核酸分子中的二级结构; 将核酸分子置于两个池中的一个中; 并且随着核酸分子的每个核苷酸通过通道进行测量，以便确定核酸分子的序列。

67. 发明授权

EP0901531B1 PROCESS FOR DIRECT SEQUENCING DURING TEMPLATE AMPLIFICATION 失效
标题翻译：程序对直接测序过程中的“模板”放大
公开(公告)号：EP0901531B1
公开(公告)日：2001-12-05
申请号：EP97924670.9
申请日：1997-05-09
申请人： SEQUENOM, INC.
发明人： KÖSTER, Hubert , VAN DEN BOOM, Dirk , RUPPERT, Andreas
IPC分类号： C12Q1/68 , C07H19/00
CPC分类号： C12Q1/6844 , C12Q1/6872 , C12Q2565/125 , C12Q2535/101 , C12Q2525/117 , C12Q2565/518 , C12Q2521/101
摘要： Processes and kits for simultaneously amplifying and sequencing nucleic acid molecules, and performing high throughput DNA sequencing are described.

68. 发明公开

EP1153140A1 PROTECTION PROBES 有权转让
标题翻译：保护探头
公开(公告)号：EP1153140A1
公开(公告)日：2001-11-14
申请号：EP00911770.6
申请日：2000-02-11
申请人： Gen-Probe Incorporated
发明人： BRENTANO, Steven, T. , MCDONOUGH, Sherrol, H. , NELSON, Norman, C.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q1/6806 , C12Q1/6832 , C12Q2527/125 , C12Q2527/107 , C12Q2525/117 , C12Q2563/103 , C12Q2561/108 , C12Q2537/113 , C12Q2537/162
摘要： The present invention features compositions and methods that are useful for storing labeled detection probes and detecting whether a target nucleic acid sequence is present in a sample. Preferred compositions are made up of a detection probe containing a label susceptible to a chemical or enzymatic alteration and a protection probe that protects the label from alteration and/or decreases the ability of the detection probe to inhibit nucleic acid amplification. Such compositions can be used, for example, to stabilize a detection probe label and to prevent a detection probe from hybridizing prematurely to amplified or target nucleic acid.

69. 发明公开

EP1097939A3 Method for the synthesis of isoguanosine and its 2'-derivatives 失效
标题翻译：一种用于2'-异鸟苷及其衍生物的制备方法
公开(公告)号：EP1097939A3
公开(公告)日：2001-05-30
申请号：EP01100768.9
申请日：1995-08-30
申请人： Bayer Corporation
发明人： Collins, Mark , Horn, Thomas , Sheridan, Patrick , Warner, Brian , Urdea, Michael
IPC分类号： C07H19/16
CPC分类号： C12N15/113 , C07H19/16 , C07H19/20 , C07H21/00 , C12N2310/322 , C12N2310/336 , C12Q1/68 , C12Q1/6811 , C12Q1/6813 , C12Q1/682 , C12Q1/6832 , C12Q1/6837 , C12Q2527/107 , C12Q2525/117 , C12Q2525/113
摘要： A method for the chemical synthesis of isoguanosine or 2'-modified isoguanosine starting from the corresponding guanosine derivative is provided.

70. 发明公开

EP0910664A1 SYNTHESIS OF FLUOROPHORE-LABELED DNA 失效
标题翻译：荧光团标记的DNA的合成
公开(公告)号：EP0910664A1
公开(公告)日：1999-04-28
申请号：EP97922346.0
申请日：1997-04-15
申请人： UNIVERSITY OF SOUTHERN CALIFORNIA , THE GOVERNORS OF THE UNIVERSITY OF ALBERTA
发明人： GOODMAN, Myron, F. , REHA-KRANTZ, Linda
IPC分类号： C12N9 , C12Q1
CPC分类号： C12Q1/6848 , C12N9/1252 , C12Q1/686 , C12Q1/6874 , Y10T436/143333 , Y10T436/24 , C12Q2563/107 , C12Q2525/101 , C12Q2525/117 , C12Q2565/102 , C12Q2565/518 , C12Q2537/157 , C12Q2521/101 , C12Q2525/301
摘要： Sequencing methods and methods for synthesizing DNA probes using mutant bacteriophage T4 DNA polymerases which have increased ability to incorporate modified nucleotides for the synthesis of long or short chains of complementary, modified, e.g., fluorophore-labeled DNA. In general, the mutant T4 DNA polymerases retain 3'→5' exonuclease activity; hence, reduction or elimination of 3'→5' exonuclease activity is not a prerequisite for efficient synthesis of a complementary fluorophore-labeled or other modified DNA. In fact, retention of 3'→5' exonuclease activity increases accuracy of DNA replication, because these exonucleases proofread or edit the product of DNA replication.

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