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    • 57. 发明公开
    • Vibrational Microscopy Imaging System
    • Vibrationsmikroskopiebildgebungssystem
    • EP2211219A2
    • 2010-07-28
    • EP10151529.4
    • 2010-01-25
    • President and Fellows of Harvard College
    • Xie, Xiaoliang SunneyFreudinger, ChristianMin, Wei
    • G02B21/00G02B21/08G01H9/00
    • G02B21/002G01J3/10G01J3/44G01J2003/1282G01N21/65G01N2021/653G01N2021/655G01N2201/0675G02B21/082
    • A microscopy imaging system is disclosed that includes a light source system, a spectral shaper, a modulator system, an optics system, an optical detector and a processor. The light source system is for providing a first train of pulses and a second train of pulses. The spectral shaper is for spectrally modifying an optical property of at least some frequency components of the broadband range of frequency components such that the broadband range of frequency components is shaped producing a shaped first train of pulses to specifically probe a spectral feature of interest from a sample, and to reduce information from features that are not of interest from the sample. The modulator system is for modulating a property of at least one of the shaped first train of pulses and the second train of pulses at a modulation frequency. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of a train of pulses of interest transmitted or reflected through the common focal volume. The processor is for detecting a modulation at the modulation frequency of the integrated intensity of substantially all of the optical frequency components of the train of pulses of interest due to the non-lincar interaction of the shaped first train of pulses with the second train of pulses as modulated in the common focal volume, and for providing an output signal for a pixel of an image for the microscopy imaging system.
    • 公开了一种显微镜成像系统,其包括光源系统,光谱整形器,调制器系统,光学系统,光学检测器和处理器。 光源系统用于提供第一列脉冲和第二脉冲串。 光谱整形器用于对频率分量的宽带范围的至少一些频率分量的光学特性进行光谱修改,使得频率分量的宽带范围成形,产生成形的第一脉冲串,从而特别地探测来自 样本,并从样本中不感兴趣的特征中减少信息。 调制器系统用于以调制频率调制成形的第一脉冲序列和第二脉冲串中的至少一个的性质。 光学检测器用于检测通过公共焦点体积传输或反射的感兴趣的脉冲串的基本上所有的光学频率分量的积分强度。 处理器用于检测由于成形的第一脉冲序列与第二脉冲序列的非线性交互作用而导致的感兴趣的脉冲序列的基本上所有的光学频率分量的积分强度的调制频率的调制 在公共焦点体积中调制,并且为显微成像系统的图像的像素提供输出信号。