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31. 发明公开

EP2390263A1 Process for the preparation of glycosylated interferon beta 审中-公开
标题翻译： Verfahren zur Herstellung von sugaryliertem干扰素β
公开(公告)号：EP2390263A1
公开(公告)日：2011-11-30
申请号：EP11161155.4
申请日：2005-08-26
申请人： ARES TRADING S.A.
发明人： Fischer, Dina , Bernard, Alain , Ducommun, Paul , Rossi, Mara
IPC分类号： C07K14/565 , C12N5/00 , C07K1/18 , C07K1/20 , C07K1/22
CPC分类号： C12N5/0031 , A61K38/215 , C07K14/565 , C12N5/0037 , C12N5/06 , C12N15/00 , C12N2500/12 , C12N2500/32 , C12N2500/90 , C12N2500/92 , C12N2500/99 , C12N2510/02
摘要： The present invention relates to a process for the production of interferon beta, and to an interferon beta composition having a unique glycosylation pattern.
摘要翻译：本发明涉及生产干扰素β的方法，以及具有独特糖基化模式的干扰素β组合物。

32. 发明公开

EP2359864A2 Targeting Vector-Phospholipid Conjugates 有权转让
标题翻译： Zielender Vektor-Phospholipid Konjugaten
公开(公告)号：EP2359864A2
公开(公告)日：2011-08-24
申请号：EP10190163.5
申请日：2006-12-08
申请人： Bracco Suisse SA
发明人： Bussat, Philippe , Cherkaoui, Samir , Fan, Hong (Helen) , Lamy, Bernard , Nanjappan, Palaniappa , Pillai, Radhakrishna, K. , Pochon, Sibylle , Song, Bo , Swenson, Rolf, E.
IPC分类号： A61K47/48 , C07K14/00 , A61K49/22 , C07K1/18 , C07K1/20 , C12P21/04 , C07K1/00 , A61K38/16
CPC分类号： C07K14/00 , A61K47/544 , A61K49/223 , Y02A50/58
摘要： Peptide vectors having high KDR binding affinity and processes for making such vectors are provided. The peptide vectors may be conjugated to phospholipids and included in ultrasound contrast agent compositions. Such ultrasound contrast agents are particularly useful in therapeutic and diagnostic methods, such as in imaging KDR-containing tissue and in the evaluation and treatment of angiogenic processes associated with neoplastic conditions. The present invention also provides processes for the large scale production of highly pure dimeric and monomeric peptide phospholipid conjugates as well as precursor materials used to form the conjugates. The present invention further provides processes for the large scale production of highly pure peptide phospholipid conjugates which contain very low levels of TFA.
摘要翻译：提供了具有高KDR结合亲和力的肽载体和用于制备这些载体的方法。肽载体可以与磷脂结合并包含在超声造影剂组合物中。这样的超声造影剂在治疗和诊断方法中是特别有用的，例如成像含KDR的组织以及与肿瘤病症相关的血管发生过程的评估和治疗。本发明还提供大规模生产高纯度二聚体和单体肽磷脂缀合物以及用于形成共轭物的前体材料的方法。本发明还提供了大规模生产含有非常低水平的TFA的高纯度肽磷脂缀合物的方法。

33. 发明公开

EP2298788A1 Method for reconstituting a recombinant protein to its biologically active form 审中-公开
标题翻译： Verfahren zur Wiederherstellung eines biologisch-aktiven rekombinant蛋白质
公开(公告)号：EP2298788A1
公开(公告)日：2011-03-23
申请号：EP10180934.1
申请日：2002-10-01
申请人： Boehringer Ingelheim RCV GmbH & Co KG
发明人： Necina, Roman , Schlegl, Robert , Jungbauer, Alois , Machold, Christine
IPC分类号： C07K1/113 , C07K1/16 , C07K1/18 , C07K1/20 , C07K1/22 , C07K1/36
CPC分类号： C07K1/1136 , C07K1/1133 , C07K1/16 , C07K1/36
摘要： In a method for reconstituting a recombinant protein from a denatured state to its active form, a feed solution containing the recombinant protein in its denatured and/or in biologically inactive intermediate forms is subjected to a chromatographic separation process, in which the protein is reconstituted under conditions that promote refolding of the protein and the intermediate forms are separated from the refolded protein. The denatured form and/or the inactive intermediate forms of the protein are separated from the refolded protein in a continuous or quasi-continuous manner and optionally recycled to the feed solution.
摘要翻译：在将重组蛋白从变性状态重建为其活性形式的方法中，将含有其变性和/或生物惰性中间体形式的重组蛋白的进料溶液进行色谱分离过程，其中将蛋白重构在促进蛋白质重折叠和中间体形式的条件与重折叠的蛋白质分离。蛋白质的变性形式和/或无活性中间体形式以连续或准连续方式与重折叠蛋白质分离，并任选地再循环至饲料溶液中。

34. 发明授权

EP1809664B1 A NOVEL PROCESS FOR PURIFICATION OF HUMAN GROWTH HARMONE 有权
标题翻译：新方法用于纯化人生长激素
公开(公告)号：EP1809664B1
公开(公告)日：2008-05-14
申请号：EP04821383.9
申请日：2004-11-09
申请人： USV Limited
发明人： BORBHUIYA, Ahmed Monsur , RAO, Yada Madhava , RAO, Laxmi Srinivas , MAITI, Dipanwita , NIPHADKAR, Prabhakar Milind , MISHRA, Shrikant
IPC分类号： C07K14/61 , C07K1/20
CPC分类号： C07K14/61 , C07K1/20
摘要： The present invention relates to a commercial process for purification of human Growth hormone obtained by DNA recombinant technique using hydrophobic interactive chromatography, efficient to separate clipped molecules of human growth hormone present as an impurity arising due to clipping positions present on the megamolecule of human growth hormone from the target human growth hormone molecule using a phenyl column, mixture of aqueous buffers and organic solvents as an eluant, followed by desalting, lypholization and the invention also relates to the process wherein the elution of clipped molecules of human growth hormone precedes the elution of target human growth hormone to obtain hGH of purity > 99.5%.

35. 发明公开

EP1581337A4 COMPOSITION FOR SEPARATING MOLECULES 审中-公开
标题翻译：组成用于分离分子
公开(公告)号：EP1581337A4
公开(公告)日：2008-05-07
申请号：EP03777714
申请日：2003-10-20
申请人： PROMEGA CORP
发明人： SIMPSON DANIEL J , JOHNSON TONNY , FLEMMING RODERICK G
IPC分类号： B01J20/22 , B01J20/32 , B01J45/00 , C02F1/28 , C07K1/20 , C12N9/00
CPC分类号： B01D15/3828 , B01J20/103 , B01J20/28009 , B01J20/286 , B01J20/3204 , B01J20/3217 , B01J20/3219 , B01J20/3234 , B01J20/3242 , B01J20/3251 , B01J20/3259 , B01J20/3265 , B01J20/3289 , B01J20/3293 , B01J45/00 , B01J2220/58 , B01J2220/66 , C02F1/285 , C02F2101/20 , C07K1/20 , G01N2030/042
摘要： The present invention provides compositions and methods for the separation of metals or molecules such as polypeptides, nucleic acids, or endotoxins using a metal-modified solid support. The metals or molecules are isolated from a starting material using the modified solid supports of the invention. Also provided by the invention are kits that can be used in connection with the inventive methods.

36. 发明授权

EP0942930B1 PURIFICATION OF NEUROTROPHINS 失效
标题翻译：神经营养因子的清洁
公开(公告)号：EP0942930B1
公开(公告)日：2007-08-29
申请号：EP97948362.5
申请日：1997-11-14
申请人： GENENTECH, INC.
发明人： BURTON, Louis, E. , SCHMELZER, Charles, H. , BECK, Joanne, T.
IPC分类号： C07K14/405 , C07K14/48 , C07K1/20
CPC分类号： C07K14/48 , C07K14/475
摘要： Methods are provided for large scale purification of neurotrophins, including mature NGF, suitable for clinical use. The methods provide means to separate neurotrophins from various less desirable misprocessed, misfolded, size, glycosylated, or charge forms. Compositions of neurotrophins, including mature NGF, substantially free of these variants are also provided.

37. 发明公开

EP1761552A1 PROCESS FOR THE PURIFICATION OF IL-18 BINDING PROTEIN 有权转让
标题翻译：纯化IL-18结合蛋白的方法
公开(公告)号：EP1761552A1
公开(公告)日：2007-03-14
申请号：EP05756895.8
申请日：2005-06-27
申请人： ARES TRADING S.A.
发明人： KORNMANN, Henri , BAER, Gianni
IPC分类号： C07K1/14 , C07K1/20 , C07K14/715
CPC分类号： C07K14/47 , C07K14/7155
摘要： The invention relates to a process for the purification of IL-18 binding protein (IL- 18BP) from a fluid using aqueous two-phase partitioning.
摘要翻译：本发明涉及使用水性两相分配从流体中纯化IL-18结合蛋白（IL-18BP）的方法。

38. 发明公开

EP1752465A2 Linear VH-CH1-VH-CH1 heavy chain antibody fragment 失效
标题翻译： Lineares，schweresAntikörperkettenfragmentVH-CH1-VH-CH1
公开(公告)号：EP1752465A2
公开(公告)日：2007-02-14
申请号：EP06022818.6
申请日：1996-04-05
申请人： Genentech, Inc.
发明人： Rinderknecht, Ernst H. , Zapata, Gerardo A.
IPC分类号： C07K1/20
CPC分类号： C07K16/32 , A61K38/00 , C07K1/20 , C07K16/065 , C07K16/2845 , C07K2317/24 , C07K2317/31 , C07K2317/54 , C07K2317/55 , C07K2317/62 , C07K2317/622 , C07K2317/73 , C07K2317/92 , C07K2319/00 , Y10S435/803
摘要： The invention provides an antibody which comprises a linear V H -C H 1-V H -C H 1 heavy chain fragment associated with two light chain fragments, wherein the C-terminus of C H 1 is joined directly to the N-terminus of V H at the Fd-Fd junction of the heavy chain fragment, a nucleic acid encoding the heavy chain fragment, and a method for the preparation of said antibody.
摘要翻译：本发明提供了一种抗体，其包含与两条轻链片段相连的线性VH -CH 1 -V H -CH 1重链片段，其中CH 1的C末端直接连接到Vd的N-末端，重链片段的Fd结，编码重链片段的核酸，以及制备所述抗体的方法。

39. 发明公开

EP1681299A3 Methods for purifying highly anionic proteins 有权
公开(公告)号：EP1681299A3
公开(公告)日：2006-12-27
申请号：EP06006740.2
申请日：2001-03-27
申请人： Genetics Institute, LLC
发明人： Coffman, Jonathan L. , Foster, William Barry , Germain, Bonnie J. , Sun, Shujun , Robinson, Jeffrey J.
IPC分类号： C07K1/20 , C07K14/705
CPC分类号： C07K1/20 , C07K1/18 , C07K1/36 , C07K14/70596 , C07K2319/00 , C07K2319/30
摘要： The present invention provides a process for isolating and purifying highly anionic target proteins, for example, sulfated proteins. Sulfated proteins have five (5) or more, sulfations. In a preferred embodiment, the sulfated protein has six (6) sulfations, e.g. , six sulfations on N-terminal tyrosine residues, as embodied in PSGL-1 (P-selectin glycoprotein ligand).

40. 发明公开

EP1687328A4 PROCESS FOR PURIFYING PROTEINS IN A HYDROPHOBIC INTERACTION CHROMATOGRAPHY FLOW-THROUGH FRACTION 有权
标题翻译： VERFAHREN ZUR AUFREINIGUNG VON PROTEINEN IN EERER DURCHFLUSSFRAKTION AUS DER CHROMATOGRAPHIE MIT HYDROPHOBEN WECHSELWIRKUNGEN
公开(公告)号：EP1687328A4
公开(公告)日：2006-11-15
申请号：EP04796067
申请日：2004-10-22
申请人： AMGEN INC
发明人： SHUKLA ABHINAV A , GHOSE SANCHAYITA
IPC分类号： C07K14/00 , C07K1/10 , C07K1/20 , C12N9/00 , C12P21/06
CPC分类号： C07K14/00 , C07K1/20 , C07K2319/30
摘要： The present invention is a process for separating a target protein (such as a recombinant protein produced in a cell culture) from a mixture containing the target protein and contaminants (such as cell culture contaminants), by contacting the mixture with a hydrophobic adsorbent comprising branched hydrocarbon functional groups in an aqueous salt solution and collecting the unbound flow-through fraction containing the target protein. In one embodiment, the hydrophobic adsorbent may be a branched alkyl functional group. In another embodiment, the branched alkyl functional group has from 3 to 8 carbon atoms. In another embodiment, the branched alkyl functional group is a tertiary carbon atom, such as tert-butyl.
摘要翻译：本发明是一种从含有目标蛋白质和污染物（如细胞培养污染物）的混合物中分离目标蛋白质（诸如在细胞培养物中产生的重组蛋白质）的方法，通过使该混合物与包含支链的疏水吸附剂烃官能团在含水盐溶液中反应并收集含有靶蛋白的未结合的流通部分。在一个实施方案中，疏水吸附剂可以是支化烷基官能团。在另一个实施方案中，支化烷基官能团具有3至8个碳原子。在另一个实施方案中，支化烷基官能团是叔碳原子，如叔丁基。

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