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    • 32. 发明公开
    • LIQUID REFLUX HIGH-SPEED GENE AMPLIFICATION DEVICE
    • 液体回流高速基因扩增装置
    • EP2415855A1
    • 2012-02-08
    • EP10758761.0
    • 2010-03-31
    • KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGYNational University Corporation Tokyo Medical and Dental University
    • TAKEI HiroyukiTERAZONO HideyukiYASUDA Kenji
    • C12M1/00G01N21/78
    • B01L7/52B01L3/50851B01L3/50855B01L2300/185B01L2400/0622B01L2400/0633B01L2400/0644C12Q1/686F28D7/0008F28D15/00F28D2021/0077F28F27/02F28F2280/02
    • The present invention provides a liquid reflux reaction control device comprising:
      a reaction vessel having one or a plurality of wells configured to accommodate a sample;
      a heat exchange vessel provided in contact with the reaction vessel so as to conduct heat to the reaction vessel, and comprising an inlet and an outlet respectively for introducing and draining a liquid of a predetermined temperature;
      a plurality of liquid reservoir tanks provided with a temperature-controllable heat source for maintaining liquids of predetermined temperatures;
      a tubular flow channel that connects the inlet and the outlet of the heat exchange vessel with the liquid reservoir tanks;
      a pump disposed on the tubular flow channel, and configured to circulate the liquid between the heat exchange vessel and the liquid reservoir tank; and
      a switching valve disposed on the tubular flow channel, and configured to control the flow of the circulating liquid, which controls the temperature of the reaction vessel to keep a desired temperature by switching the flows of the liquids of the predetermined temperatures from the plurality of liquid reservoir tanks into the heat exchange vessel at a predetermined time interval,
      wherein the amount of the sample is less than or equal to several µL per well, and the total volume of the circulating liquid is more than or equal to several tens of mL per liquid reservoir tank.
    • 本发明提供了一种液体回流反应控制装置,包括:反应容器,具有一个或多个配置为容纳样品的孔; 设置成与反应容器接触以将热量传导至反应容器的热交换容器,并且包括分别用于引入和排出预定温度的液体的入口和出口; 设置有用于保持预定温度的液体的温度可控的热源的多个液体储存箱; 连接热交换容器的入口和出口与液体储存罐的管状流动通道; 配置在所述管状流路上并使所述液体在所述热交换容器与所述液体储存罐之间循环的泵; 以及切换阀,其配置在上述管状流路上,控制上述循环液的流动,通过从上述多个切换阀切换上述规定温度的液体的流量,来控制上述反应容器的温度以维持期望的温度 以预定的时间间隔向所述热交换容器内导入所述液体贮存槽,所述样本量为每孔数μL以下,所述循环液的总容量为数十mL / 储液罐。