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    • 22. 发明公开
    • Homomultimeric structure by assembly of SIRV2 P98 proteins or P98 variants, conjugate and uses thereof
    • 同源多聚体结构通过布置SIRV2-P98-P98蛋白或其变体,共轭物和它们的用途
    • EP2479185A1
    • 2012-07-25
    • EP11305058.7
    • 2011-01-20
    • INSTITUT PASTEUR
    • Quax, Tessa E.F.Lucas, SoizickForterre, PatrickPrangishvili, David
    • C07K14/01B82Y5/00
    • C07K14/01B82Y5/00C12N2710/00088C12N2795/10011
    • The present invention relates to a homomultimeric protein structure constituted by assembled monomers of the P98 protein of Sulfolobus islandicus rod-shaped virus 2 (SIRV2) or assembled monomer variants of said P98 protein. In a particular embodiment, this homomultimeric protein structure has a seven-fold rotational symmetry, and is found in an open conformation or closed conformation. A particular structure has the form of baseless 7-face pyramid. The invention also relates to a conjugate comprising or consisting of a homomultimeric protein structure of the invention to which one or more heterologous molecule(s) is attached. Furthermore, the invention also concerns a homomultimeric protein structure or a conjugate of the invention, inserted into or exposed at the surface of a lipid layer or bilayer, of a vesicle or of a cell, and their uses thereof.
    • 本发明涉及通过硫化islandicus棒状病毒2(SIRV2)或所述P98蛋白的组装单体变体的蛋白质P98的组装单体构成的同源多聚体的蛋白质结构。 在特定实施例中,同源多聚体本蛋白质结构具有7倍的旋转对称性,并且在开放构象或闭合构象中找到。 特定结构具有无端7-面棱锥的形式。 因此,该发明涉及一种缀合物,包含或本发明的一个同源多聚体蛋白质的结构由与其中一个或多个异源分子(S)附着。 进一步,本发明因此涉及一种同源多聚体蛋白质的结构或本发明的缀合物,插入或在囊泡或细胞的脂质层或脂质双层中的表面暴露,以及它们的用途及其。