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    • 29. 发明公开
    • DEVICE FOR USE IN ELISPOT
    • 用于ELISPOT的设备
    • EP3171170A1
    • 2017-05-24
    • EP15194955.9
    • 2015-11-17
    • Lionex GmbH
    • Singh, MahavirTruthmann, Katja
    • G01N33/52G01N33/543
    • G01N33/52G01N33/54366
    • In a first aspect, the present invention relates to the use of a device, like a microtiter plate having multiple predetermined reaction areas, like multiple wells, wherein the device with the multiple predetermined reaction areas, like the wells of the microtiter plate, do not contain any membrane or other structure present in or on the predetermined reaction areas, e.g. fitted into the well, for use in an enzyme-linked immunospot (ELISPOT) assay. Surprisingly, the inventors identified that the ELISPOT assay can be performed using simple devices the the multiple predetermined reaction areas, like the microtiter plates without the need of any kind of membrane or other structure present in or on the reaction areas of the deivce, like fitted into the well, for increasing surface area etc. In a further aspect, the present invention relates to an ELISPOT assay based method for determining compounds secreted from culture cells whereby the culturing of the culture cells is conducted in or on the device, like in the microtiter plates according to the present invention. Furthermore, a test kit for use in an ELISPOT assay comprising the devices, like the microtiter plates according to the present invention are provided.
    • 在第一方面,本发明涉及的装置的使用,如具有多个预定的反应区域,例如多个孔微量滴定板,其中与所述多个预定的反应区域中的设备,如微量滴定板的孔中,不要 含有存在于预定反应区域中或上的任何膜或其他结构, 装入孔中,用于酶联免疫斑点(ELISPOT)分析。 令人惊讶的是,本发明人确定了ELISPOT测定可以使用简单的装置进行,即多个预定反应区域,如微量滴定板,而不需要存在于设备的反应区域中或其上的任何种类的膜或其他结构, 进入孔中,增加表面积等。另一方面,本发明涉及用于确定从培养细胞分泌的化合物的基于ELISPOT测定的方法,由此培养细胞在装置中或装置上进行,如 根据本发明的微量滴定板。 此外,提供了用于ELISPOT测定的测试试剂盒,其包括如本发明的微量滴定板那样的装置。