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    • 21. 发明公开
    • Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
    • 纤维素降解酶,核酸编码这些,以及它们的制备和使用过程
    • EP2450439A1
    • 2012-05-09
    • EP11007476.2
    • 2006-12-08
    • Verenium Corporation
    • Gray, Kevin A.Zhao, LishanCayouette, Michelle H.
    • C12N9/42
    • C12N9/2437C12N9/2402C12N9/2405C12N9/248C12Y302/01004C12Y302/01006C12Y302/01008C12Y302/01021C12Y302/01023C12Y302/01039C12Y302/01055C12Y302/01091Y02E50/16Y02E50/17Y10T428/249921
    • The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a β-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.
    • 本发明提供具有任何纤维素分解活性,例如纤维素酶活性,内切葡聚糖酶,纤维二糖水解,β-葡糖苷酶,木聚糖酶,甘露聚糖酶,阿拉伯呋喃糖酶在一个²木糖苷多肽,和/或寡聚体酶活性,多核苷酸编码本文 多肽,以及制备和使用这些多核苷酸和多肽的方法。 在一个方面,本发明涉及具有任何纤维素分解活性,例如纤维素酶活性,例如内切葡聚糖酶,纤维二糖水解,β-葡糖苷酶,木聚糖酶,甘露聚糖酶,²木糖苷酶,阿拉伯呋喃糖苷酶和/或寡聚体酶活性,包括热电表多肽和 耐热活性,多核苷酸编码合成的酶,以及制备和使用这些多核苷酸和多肽。 在一个方面,本发明提供具有对寡聚体酶活性的多肽,E. G.,酶没有转换顽抗可溶性寡聚体可发酵的糖在生物质的糖化。 本发明的多肽可以在多种药物,农业,食品和饲料加工和工业环境中使用。 因此本发明提供的组合物或包含酶包含本发明的至少一种酶的混合物制造的产品。