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21. 发明授权

EP2623586B1 Compositions and methods for surface treatment with lipases 有权
公开(公告)号：EP2623586B1
公开(公告)日：2017-08-16
申请号：EP13153352.3
申请日：2013-01-30
申请人： The Procter & Gamble Company
发明人： Lant, Neil Joseph , Erlandsen, Luise , Hansen, Carsten Hoerslev , Vind, Jesper , Svendsen, Allan , Sönksen, Carsten Peter
IPC分类号： C11D3/39 , C12N9/20 , C11D3/386 , C11D3/395
CPC分类号： C11D3/38627 , C11D3/392 , C11D3/3927 , C11D3/3947 , C11D3/3951 , C12N9/20

22. 发明公开

EP3201305A1 LIPASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 有权
标题翻译：脂肪酶变体和编码相同的多核苷酸
公开(公告)号：EP3201305A1
公开(公告)日：2017-08-09
申请号：EP15770526.0
申请日：2015-09-28
申请人： Novozymes A/S
发明人： HANSEN, Carsten Hoerslev , VIND, Jesper , ERLANDSEN, Luise , PLESNER, Bitten , STENHOLM, Anna Catharina Olsen
IPC分类号： C11D3/386 , C12N9/00
CPC分类号： C11D3/386 , C11D3/38627 , C11D3/38681 , C11D3/392 , C12N9/00 , C12N9/16 , C12N9/20 , C12Y301/00
摘要： The present invention relates to lipase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
摘要翻译：本发明涉及脂肪酶变体。本发明还涉及编码变体的多核苷酸; 包含所述多核苷酸的核酸构建体，载体和宿主细胞; 和使用这些变体的方法。

23. 发明授权

EP2834353B1 LIPASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME 有权
标题翻译：脂肪酶变体和编码相同的多核苷酸
公开(公告)号：EP2834353B1
公开(公告)日：2017-07-19
申请号：EP13711677.8
申请日：2013-03-22
申请人： Novozymes A/S
发明人： SKJOLD-JOERGENSEN, Jakob , VIND, Jesper , SVENDSEN, Allan
IPC分类号： C12N9/20 , C12N15/00
CPC分类号： C12N9/18 , C11D3/38627 , C12N1/14 , C12N1/20 , C12N9/20 , C12N15/52 , C12N15/63
摘要： The present invention relates to isolated lipase variants, comprising a substitution at one or more positions corresponding to positions I86D,E,N,Q, E87C, I90D,E,Q, and N92D,E,Q of the mature polypeptide of SEQ ID NO: 2, wherein the variant has lipase activity. In some embodiments the present invention relates to isolated polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; methods of producing the variants; and compositions comprising the variants. The present invention also relates to methods of obtaining lipase variants; methods of cleaning; and use of lipase variants for cleaning.
摘要翻译：本发明涉及分离的脂肪酶变体，其包含在对应于SEQ ID NO的成熟多肽的位置I86D，E，N，Q，E87C，I90D，E，Q和N92D，E，Q的一个或多个位置处的取代：2，其中该变体具有脂肪酶活性。在一些实施方案中，本发明涉及编码变体的分离的多核苷酸; 包含所述多核苷酸的核酸构建体，载体和宿主细胞; 生产变体的方法; 和包含这些变体的组合物。本发明还涉及获得脂肪酶变体的方法; 清洁方法; 并使用脂肪酶变体进行清洁。

24. 发明公开

EP3190182A1 PHOSPHOLIPASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM 无效
标题翻译：磷脂酶，编码它们的核酸以及制备和使用它们的方法
公开(公告)号：EP3190182A1
公开(公告)日：2017-07-12
申请号：EP16184319.8
申请日：2005-03-08
申请人： DSM IP Assets B.V.
发明人： GRAMATIKOVA, Svetlana , HAZLEWOOD, Geoff , LAM, David , BARTON, Nelson R. , STURGIS, Blake G. , ROBERTSON, Dan E. , LI, Jincai , KREPS, Joel A. , FIELDING, Roderick , BROWN, Robert C. , VASAVADA, Amit , TAN, Xuqiu , BADILLO, Adrian , VAN HOEK, Wilhelmus P. , JANSSEN, Giselle , ISAAC, Charles , BURK, Mark J.
IPC分类号： C12N9/20 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N15/8242 , C07K2319/00 , C12N9/16 , C12N9/18 , C12N9/20 , C12N15/8247 , C12P7/6418 , C12P7/6481
摘要： The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, phosphatidic acid phosphatases (PAP)) and/or lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e.g., oil degumming, and products comprising use of these phospholipases are also provided.
摘要翻译：本发明提供了具有磷脂酶活性的新型多肽，包括例如磷脂酶A，B，C和D活性，patatin活性，磷脂酸磷酸酶（PAP））和/或脂质酰基水解酶（LAH）活性，编码它们的核酸和抗体绑定到他们。还提供了工业方法，例如油脱胶，以及包含使用这些磷脂酶的产品。

25. 发明公开

EP3181689A1 METHOD FOR PRODUCING SUBSTRATE SOLUTION FOR LIPASE ACTIVITY ASSAY, AND METHOD FOR SIMPLIFYING PRODUCTION 有权转让
标题翻译：一种用于生产底物溶液LIPASEAKTIVITÄTSTEST和方法，便利进行
公开(公告)号：EP3181689A1
公开(公告)日：2017-06-21
申请号：EP15832348
申请日：2015-08-07
申请人： SHINO-TEST CORP
发明人： IIZUKA NAOMI , HIKICHI ATSUSHI
IPC分类号： C12N9/20
CPC分类号： C12Q1/44 , C12N9/20 , C12Y301/01003 , G01N2333/92
摘要： Provided is a process for producing a substrate solution for measuring lipase activity including, as a substrate for measuring lipase activity, 1,2-o-dilauryl-rac-glycero-3-glutaric acid (6'-methylresorufin) ester, wherein the production process does not necessitate cumbersome or special processing such that skill is required, or does not necessitate a special apparatus, instruments, or other items. This production process is a process for producing a substrate solution for measuring lipase activity, and is characterized by including the steps of (1) mixing the substrate for measuring lipase activity and a side-chain-type nonreactive polyether-modified-type modified silicone oil or a polyoxyethylene/polyoxypropylene condensate to prepare a mixture, and (2) mixing all or a portion of the mixture of step (1) with water or an aqueous solution.
摘要翻译：本发明提供一种制备用于测定脂酶活性，包括一个底物溶液，作为测定脂肪酶活性，1,2-O-二月桂基 - 外消旋 - 甘油-3-戊二酸（6'- methylresorufin）酯基板，worin生产过程中不必要笨重或特殊处理进行检查并需要技巧，或者不必要特别的装置，仪器，或其他物品。本制法是用于生产用于测定脂肪酶活性的底物溶液的方法，以及通过包括（1）将基片，用于测量脂肪酶活性和侧链型非反应性聚醚改性型改性硅油的步骤为特征的或聚氧乙烯/聚氧丙烯缩合物，以制备混合物，和（2）混合或全部与水工序（1）的混合物的一部分或到wässrige溶液。

26. 发明公开

EP3081644A4 MODIFIED LIPASE AND USE THEREOF 有权
标题翻译：维多利亚州的维多利亚州
公开(公告)号：EP3081644A4
公开(公告)日：2017-05-24
申请号：EP14869024
申请日：2014-12-08
申请人： AMANO ENZYME INC
发明人： ISHIGAKI YUKI , ISHIHARA SATORU , TANAKA SHUN-ICHI
IPC分类号： C12N15/09 , A23C9/152 , A23L27/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/20
CPC分类号： C12N9/20 , A23C9/1216 , A23L27/25 , A23V2002/00 , C12Y301/01003
摘要： The present invention addresses a problem of providing a lipase derived from a microorganism that is specific for short-chain to medium-chain fatty acids. A modified lipase is obtained by making a substitution in the amino acid sequence of a Candida cylindracea derived lipase, wherein the substitution is (1) a substitution of asparagine for an amino acid corresponding to the amino acid at position 428 in the amino acid sequence set forth in SEQ ID NO: 1; or (2) a substitution of phenylalanine, methionine, or isoleucine for an amino acid corresponding to the amino acid at position 429 in the amino acid sequence set forth in SEQ ID NO: 1.
摘要翻译：本发明涉及一种提供来源于短链中链链脂肪酸特异性微生物的脂肪酶的问题。修饰的脂肪酶是通过在圆假丝酵母衍生的脂肪酶的氨基酸序列中进行取代而获得的，其中取代是（1）天冬酰胺取代对应于氨基酸序列组中428位氨基酸的氨基酸在SEQ ID NO：1中; 或（2）苯丙氨酸，甲硫氨酸或异亮氨酸取代对应于SEQ ID NO：1所示氨基酸序列中第429位氨基酸的氨基酸。

27. 发明授权

EP2371949B1 Lipase variants 有权
标题翻译：脂肪酶变体
公开(公告)号：EP2371949B1
公开(公告)日：2017-04-19
申请号：EP10180310.4
申请日：2007-01-22
申请人： Novozymes A/S , Novozymes Inc.
发明人： Vind, Jesper , Knötzel, Jürgen Carsten Franz , Borch, Kim , Svendsen, Allan , Callisen Thomas Honger , Yaver, Debbie , Bjoernvad, Mads Eskelund , Hansen, Peter Kamp , Ge, Haiyan , Lamsa, Michael
IPC分类号： C12N9/20 , C12N9/18
CPC分类号： C12N9/20 , C12N9/18

28. 发明授权

EP2767586B1 NOVEL PROCESS FOR ENZYMATIC ACRYLAMIDE REDUCTION IN FOOD PRODUCTS 有权
标题翻译：方法中丙烯酰胺含量的食物中酶法羞辱
公开(公告)号：EP2767586B1
公开(公告)日：2017-03-22
申请号：EP14168497.7
申请日：2006-05-29
申请人： DSM IP Assets B.V.
发明人： De Boer, Lex
IPC分类号： C12N9/18 , C12N9/20 , C12N9/24 , C12N9/26 , C12N9/42 , C12N9/82 , A21D8/04
CPC分类号： A21D8/042 , C12N9/18 , C12N9/20 , C12N9/2402 , C12N9/2414 , C12N9/2437 , C12N9/82 , C12Y301/01001 , C12Y301/01003 , C12Y302/01001 , C12Y302/01004 , C12Y305/01001

29. 发明公开

EP3122874A1 POLYPEPTIDES HAVING PHOSPHOLIPASE C ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 审中-公开
标题翻译：多肽麻醉剂磷脂酶C-AKTIVITÄTUNDDAFÜRCODIERENDE POLYNUKLEOTIDE
公开(公告)号：EP3122874A1
公开(公告)日：2017-02-01
申请号：EP15767917.6
申请日：2015-03-27
申请人： Novozymes A/S
发明人： SUN, Tianqi , LI, Ming , BORCH, Kim , NOERGAARD, Allan , BRASK, Jesper , DAMSTRUP, Marianne Linde , LILBAEK, Hanna Maria
IPC分类号： C12N9/20 , C12N1/20 , C12N15/00 , C07H21/00 , C07K14/37 , C11B3/00
CPC分类号： C11B3/003 , C07H21/00 , C12N9/20 , C12N15/52 , C12Y301/04003
摘要： The present invention provides polypeptides having phospholipase C activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. Furthermore, the present invention provides a method of reducing the phospholipid content in an oil composition using the polypeptide having phospholipase C activity as well as other relevant uses of the polypeptide.
摘要翻译：本发明提供了具有磷脂酶C活性的多肽和编码该多肽的多核苷酸。还提供了包含多核苷酸的核酸构建体，载体和宿主细胞以及产生多肽的方法。此外，本发明提供使用具有磷脂酶C活性的多肽以及多肽的其它相关用途降低油组合物中磷脂含量的方法。

30. 发明公开

EP3113625A1 ZUSAMMENSETZUNG FÜR DIE ENZYMATISCHE ÖLENTSCHLEIMUNG 审中-公开
标题翻译： ZUSAMMENSETZUNGFÜRDIE ENZYMATISCHEÖLENTSCHLEIMUNG
公开(公告)号：EP3113625A1
公开(公告)日：2017-01-11
申请号：EP15705030.3
申请日：2015-02-19
申请人： Clariant Produkte (Deutschland) GmbH
发明人： SOHLING, Ulrich , SUCK, Kirstin , BUBENHEIM, Paul
IPC分类号： A23L5/20 , C11B3/00 , C11B3/04 , C11B3/16
CPC分类号： C11B3/003 , A23D9/04 , A23L5/25 , A23V2002/00 , C11B3/001 , C11B3/006 , C11B3/04 , C11B3/16 , C12N9/16 , C12N9/20 , C12N9/52 , C12N9/54 , C12Y301/01003 , C12Y301/01004 , C12Y301/01032 , C12Y301/04003 , C12Y301/04004 , C12Y304/11 , C12Y304/13 , C12Y304/14 , C12Y304/15 , C12Y304/16 , C12Y304/17 , C12Y304/18 , C12Y304/19 , C12Y304/23 , C12Y304/25
摘要： The invention relates to a composition comprising at least one phospholipid-splitting enzyme and at least one protease. The invention further relates to a method for degumming triglyceride-containing compositions by use of the composition according to the invention and to the use of said composition for degumming triglyceride-containing compositions.
摘要翻译：本发明涉及包含至少一种磷脂裂解酶和至少一种蛋白酶的组合物。本发明还涉及通过使用根据本发明的组合物使含甘油三酯组合物脱胶的方法以及所述组合物用于脱胶含甘油三酯组合物的用途。

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