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    • 21. 发明公开
    • Carboxylic acid derivatives and their synthesis method
    • Carbonsäurederivateund ihr Syntheseverfahren
    • EP1067112A2
    • 2001-01-10
    • EP99307985.4
    • 1999-10-11
    • Korea Kumho Petrochemical Co. Ltd.
    • Seo, Dong ChulPark, Joo HyeonKim, Jae YoungKim, Seong Ju
    • C07C69/75C07C67/11C07C69/753C07C69/608C07J9/00C07C69/00
    • G03F7/0045C07C67/11C07C69/608C07C69/75C07C69/753C07C2601/14C07C2602/42C07C2603/74C07J9/005G03F7/0392G03F7/0395G03F7/0397
    • This invention relates to novel carboxylic acid derivatives represented by the following formula I and their synthesis:
         wherein, R 1 is an hydrogen atom, an alkyl group or an alkoxy group of 1 to 20 carbon atoms in a linear, branched or cyclic form; R 2 is a carboxy group of 1 to 40 carbon atoms in a linear, branched or cyclic form which is unsubstituted, or substituted into a hydroxy group, an ester group and an ether group.
      The novel carboxylic acid derivatives are more easily decomposed by acid than t-butyl ester compounds but are not dissolved in basic aqueous solution. According to this invention, carboxylic acid is under condensation with halogen compounds designed to prepare a larger monomolecular compound compared to the conventional method. Further, the condensed site is easily decomposed by acid but is extremely insoluble by basic aqueous solution. The carboxylic acid derivatives in a photoresist composition function not only as a dissolution promoter in the exposed area due to formation of the carboxylic aicd, thus enhancement of etching resistance and pattern profile in a resist.
    • 本发明涉及由下式I表示的新型羧酸衍生物及其合成:其中,R 1为直链状,支链状或环状的1〜20个碳原子的氢原子,烷基或烷氧基 ; R2是直链状,支链状或环状的1〜40个碳原子的羧基,未取代或取代为羟基,酯基和醚基。 新型羧酸衍生物比叔丁酯化合物更容易被酸分解,但不溶于碱性水溶液。 根据本发明,与常规方法相比,羧酸与设计用于制备较大单分子化合物的卤素化合物缩合。 此外,凝聚位点容易被酸分解,但由碱性水溶液极难溶解。 光致抗蚀剂组合物中的羧酸衍生物由于羧酸形成而不仅作为曝光区域中的溶解促进剂,因此抗蚀剂中的耐腐蚀性和图案特性的增强。
    • 23. 发明公开
    • Isopentenyl diphosphate isomerase from Hevea Brasiliensis and rubber producing method using the same
    • Kautschukherstellungsverfahren unter Benutzung von异戊烯基二磷酸异构酶von Hevea Brasiliensis
    • EP1046709A1
    • 2000-10-25
    • EP99303130.1
    • 1999-04-22
    • Korea Kumho Petrochemical Co. Ltd.
    • Han, Kyung-Whan c/o Dept. of ForestryKang, Hun-SeungOh, Soo-KyungShin, Dang-HoYang, Jae-Mo
    • C12N15/61C12N9/90C12N15/29C12N15/63C12N5/10C12P5/02
    • C12P5/007C12N9/90
    • The present invention cloned a cDNA clone encoding isopentenyl diphosphate (hereafter "IPP") isomerase (EC 5.3.3.2) from a cDNA library of Hevea brasiliensis latex. The clone has a continuous open reading frame encoding a peptide of 234 amino acids with a predicted molecular mass of 26.7 kDa. The deduced protein is acidic with an isoelectric point of 4.7 and shows high sequence identity with other IPP isomerases. The recombinant protein expressed in Escherichia coli showed IPP isomerase activity. In vitro rubber biosynthesis assays using washed rubber particle (WRP) deprived of initiating allylic diphosphates were performed with the addition of IPP isomerase in the reaction mixture. Results revealed that the recombinant IPP isomerase is catalytically active in catalyzing the conversion of IPP to DMAPP, a key activation step of the basic five-carbon isoprene unit in rubber biosynthesis. Southern analysis indicated that the IPP isomerase is encoded by two genes in Hevea rubber tree. In Northern blot analysis, two different sizes of transcripts (1.2 and 0.6 kb) were detected from leaf tissues while only one hybridizing band (1.0 kb) was detected from latex. Analyses of RNA extracted from extruded latex and leaf tissues of the trees wounded with nails showed that wounding did not change the transcript level of IPP isomerase.
    • 编码来自说明书中给出的234个氨基酸的序列(I)的双酚A的异戊基二磷酸(IPP)异构酶的核酸(A)是新的。 还包括以下独立权利要求:(1)在说明书中给出的具有1288个核苷酸序列(II)的核酸; (2)在45℃下用0.45M NaCl,0.04M柠檬酸钠和0.1%十二烷基硫酸钠洗涤严格性表示的条件下与(A)或(1)杂交的分离的核酸; (3)包含(A)或(1)的载体; (4)转化宿主,其包含能够表达核酸的(3) (5)在体外生产橡胶以将核酸分子转移到宿主细胞以产生转化的宿主细胞,并在适于生产从三聚体分离的IPP异构酶的培养基中的生物体内的适合于橡胶生产的环境中培养转化的宿主 用于催化体外合成橡胶的巴西利亚(IPIHb)。