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    • 15. 发明公开
    • MICRO FLOW FILTRATION SYSTEM AND FLOW FILTRATION METHOD FOR A FLUID SAMPLE
    • 流感病毒防疫系统FLUISFILTRATIONVERFAHRENFÜREINEFLÜSSIGKEITSPROBE
    • EP2830743A1
    • 2015-02-04
    • EP13711904.6
    • 2013-03-25
    • Roche Diagnostics GmbHF.Hoffmann-La Roche AG
    • ORANTH, NorbertLOSLEBEN, NadineLUTZ, SaschaGROSSMANN, Adelbert
    • B01D61/14B01D61/18
    • B01D61/20B01D61/145B01D61/18B01D61/22B01D63/088B01D2313/243B01D2313/90B01D2315/10
    • A flow filtration system (1) for concentration of components contained in a fluid sample comprises a fluid channel (2) being formed by two conduits (4, 5) allowing a bidirectional flow of the fluid sample through the fluid channel (2), a tangential flow filtration module (3) and at least two pairs (8 ,9) of piston pumps (6) each having two piston pumps (6), wherein the piston pumps (6) having a piston swept volume forming a reservoir being able to contain the fluid for volumes of up to 100 ml. The tangential flow filtration module (3) is located in the fluid channel (2) so that a fluid flowing through the channel (2) passes through the filtration module (3). At each end (10, 11) of the channel (2) one piston pump (6) of each pair (8, 9) of piston pumps (6) is located in such a manner that the piston pumps are fluidically connected in parallel. The system (1) is arranged and adapted such that during the concentration process at least for a predetermined period of time only one of the pairs (8, 9) of piston pumps (6) is used to drive the fluid through the tangential flow filtration module (3).
    • 用于浓缩包含在流体样品中的组分的流过滤系统(1)包括由允许流体样品​​通过流体通道(2)的双向流动的两个管道(4,5)形成的流体通道(2), 切向流过滤模块(3)和具有两个活塞泵(6)的活塞泵(6)的至少两对(8,9),其中具有形成储存器的活塞扫过体积的活塞泵(6)能够 含有高达100ml体积的流体。 切向流过滤模块(3)位于流体通道(2)中,使得流过通道(2)的流体通过过滤模块(3)。 在通道(2)的每个端部(10,11)处,活塞泵(6)的每对(8,9)的一个活塞泵(6)以这样的方式定位,使得活塞泵流体地并联连接。 系统(1)的布置和调整使得在浓缩过程中至少在预定的时间段内,仅使用活塞泵(6)的成对(8,9)中的一个来驱动流体通过切向流过滤 模块(3)。
    • 19. 发明公开
    • Micro flow filtration system and flow filtration method
    • Mikroflussfiltrations系统和Flussfiltrationsverfahren
    • EP2644259A1
    • 2013-10-02
    • EP12162012.4
    • 2012-03-29
    • Roche Diagnostics GmbHF. Hoffmann-La Roche AG
    • The designation of the inventor has not yet been filed
    • B01D61/18B01D61/20
    • B01D61/22B01D61/147B01D61/18B01D61/20B01D2311/14B01D2313/10B01D2313/14B01D2313/243B01D2313/28B01D2313/50B01D2315/10B01D2315/12
    • A micro flow filtration system comprises a fluid circuitry 3 and a first reservoir 1 outside the circuitry 3 suitable for containing a fluid. The fluid circuitry 3 comprises a tangential flow filtration module 10 capable of separating the fluid sample into a retentate stream and a permeate stream upon passage of the fluid sample into the tangential flow filtration module 10 through an inlet feed 18. The fluid circuitry 3 further comprises a second reservoir 2 integrated in the fluid circuitry 3, a pump 5 for creating and driving a fluid flow, optionally at least one pressure sensor 6, 7 or 8 for acquiring and detecting data about the fluid sample, optionally a pressure regulator 9 for regulating the flow in the fluid circuitry 3 and a plurality of conduits 22 forming the fluid circuitry 3 together with the second reservoir 2, the TFF-module 10, the pump 5, the pressure sensor 6, 7 or 8 (if present) and the pressure regulator 9 (if present). The volume of the first reservoir 1 outside the circuitry 3 is significantly larger than the volume of the second reservoir 2. The first reservoir 1 outside the fluid circuitry 3 is connected to the circuitry 3 such that the fluid flows unidirectionally into the circuitry 3 until the first reservoir 1 is empty so that a continuous fluid flow from the first reservoir 1 to the circuitry 3 is established.
    • 微流过滤系统包括流体回路3和适于容纳流体的回路3外部的第一储存器1。 流体回路3包括切向流过滤模块10,该切向流过滤模块10能够通过入口进料18将流体样品分离成滞留物流和通过流体样品进入切向流过滤模块10.流体回路3还包括 集成在流体回路3中的第二储存器2,用于产生和驱动流体流的泵5,可选地至少一个用于获取和检测关于流体样品的数据的压力传感器6,7或8,可选地用于调节 流体回路3中的流动以及与第二储存器2,TFF模块10,泵5,压力传感器6,7或8(如果存在的话)一起形成流体回路3的多个导管22和压力 调节器9(如果存在)。 电路3外部的第一储存器1的体积显着大于第二储存器2的体积。流体回路3外部的第一储存器1连接到电路3,使得流体单向流入电路3,直到 第一储存器1是空的,从而建立从第一储存器1到电路3的连续流体流动。