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91. 发明公开

EP2368982A2 Methods and compositions for using zinc finger endonucleases to enhance homologous recombination 审中-公开
标题翻译：方法和组合物使用的锌指内切核酸酶用于同源重组的改进
公开(公告)号：EP2368982A2
公开(公告)日：2011-09-28
申请号：EP11165685.6
申请日：2003-03-20
申请人： Sangamo BioSciences, Inc.
发明人： Liljedahl, Monika , Aspland, Simon Eric , Segal, David J.
IPC分类号： C12N9/22 , C12N15/63 , C12N15/82 , C12N15/85 , C12N15/90 , A01K67/027
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2217/075 , C07K2319/00 , C12N9/22 , C12N15/8213 , C12N15/907
摘要： Embodiments relate to methods of generating a genetically modified cell. The methods can include providing a primary cell containing an endogenous chromosomal target DNA sequence in which it is desired to have homologous recombination occur. The methods also can include providing a zinc finger endonuclease (ZFE) that includes an endonuclease domain that cuts DNA, and a zinc finger domain that includes a plurality of zinc fingers that bind to a specific nucleotide sequence within the endogenous chromosomal target DNA in the primary cell. Further, the methods can include contacting the endogenous chromosomal target DNA sequence with the zinc finger endonuclease in the primary cell such that the zinc finger endonuclease cuts both strands of a nucleotide sequence within the endogenous chromosomal target DNA sequence in the primary cell, thereby enhancing the frequency of homologous recombination in the endogenous chromosomal target DNA sequence. The methods also include providing a nucleic acid comprising a sequence homologous to at least a portion of said endogenous chromosomal target DNA such that homologous recombination occurs between the endogenous chromosomal target DNA sequence and the nucleic acid.
摘要翻译：实施例涉及产生遗传修饰的细胞的方法。该方法可以包括提供包含在其中期望具有的同源重组好发源染色体靶DNA序列的一个主小区。所述方法因此可包括提供一个锌指内切核酸酶（ZFE）确实包括在核酸内切酶结构域确实切割DNA，和一个锌指结构域也包括锌指的多元性没有绑定到特定核苷酸序列中的主要内源性染色体靶DNA内细胞。此外，该方法可包括使与所述主小区搜索的锌指内切核酸酶的内源染色体靶DNA序列，DASS死锌指内切核酸酶剪切的核苷酸序列的两条链的内源染色体靶DNA序列内的主小区，从而提高了内源染色体靶DNA序列中的同源重组的频率。因此，该方法包括提供核酸，其包含的序列同源的至少测试所述内源染色体靶DNA的一部分做了同源重组的染色体内源性靶DNA序列和核酸之间的发生。

92. 发明公开

EP2297318A1 COMPOSITIONS FOR LINKING DNA-BINDING DOMAINS AND CLEAVAGE DOMAINS 有权转让
标题翻译： ZUSAMMENSETZUNGEN ZUMVERKNÜPFENVON DNA-BINDUNGSDOMÄNENUNDSPALTUNGSDOMÄNEN
公开(公告)号：EP2297318A1
公开(公告)日：2011-03-23
申请号：EP09766999.8
申请日：2009-05-28
申请人： Sangamo BioSciences, Inc.
发明人： MILLER, Jeffrey, C.
IPC分类号： C12N15/09 , C12N9/22
CPC分类号： C12N9/22 , C07K2319/00 , C07K2319/80 , C12N15/907
摘要： Disclosed herein are compositions for linking DNA binding domains and cleavage domains (or cleavage half-domains) to form non-naturally occurring nucleases. Also described are methods of making and using compositions comprising these linkers.
摘要翻译：本文公开了用于连接DNA结合结构域和切割结构域（或切割半结构域）以形成非天然存在的核酸酶的组合物。还描述了制备和使用包含这些接头的组合物的方法。

93. 发明公开

EP2206782A1 Methods and compositions for gene inactivation 审中-公开
标题翻译： Verfahren und Zusammensetzungen zur Gendeaktivierung
公开(公告)号：EP2206782A1
公开(公告)日：2010-07-14
申请号：EP10152257.1
申请日：2007-05-23
申请人： Sangamo BioSciences, Inc.
发明人： Ando, Dale , Holmes, Michael Christopher , Lee, Gary Ka Leong
IPC分类号： C12N15/113 , C07K14/715
CPC分类号： C12N9/22 , A61K38/00 , A61K48/00 , C07K14/47 , C07K14/4703 , C12N15/86 , C12N2710/10343 , C12N2710/10345
摘要： Disclosed herein are methods and compositions for inactivating CCR-5 genes, using zinc finger nucleases (ZFNs) comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding ZFNs, vectors comprising polynucleotides encoding ZFNs, such as adenovirus (Ad) vectors, and cells comprising polynucleotides encoding ZFNs and/or cells comprising ZFNs are also provided.
摘要翻译：本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的锌指核酸酶（ZFN）来灭活CCR-5基因的方法和组合物。还提供了编码ZFN的多核苷酸，包含编码ZFN的多核苷酸的载体，例如腺病毒（Ad）载体，以及包含编码ZFN和/或包含ZFN的细胞的多核苷酸的细胞。

94. 发明公开

EP2155873A2 METHODS AND COMPOSITIONS FOR INCREASED TRANSGENE EXPRESSION 有权转让
标题翻译：方法和组合物增强转基因表达
公开(公告)号：EP2155873A2
公开(公告)日：2010-02-24
申请号：EP08754665.1
申请日：2008-05-22
申请人： Sangamo BioSciences, Inc.
发明人： HOLMES, Michael C. , LEE, Gary Ka Leong
IPC分类号： C12N15/00
CPC分类号： C12N15/86 , C12N5/0636 , C12N15/63 , C12N2501/2315 , C12N2501/51 , C12N2501/515 , C12N2510/02 , C12N2710/10343
摘要： Described herein are methods of expressing nucleic acids in T cells pre-exposed to a co-stimulatory signal and then transduced with adenoviral vectors. In some embodiments, the co-stimulation is provided by anti-CD3 and anti-CD28 antibodies and the adenoviral vector is pseudotyped for T-cell entry. The invention also relates to compositions for carrying out these methods, provided as kits or pharmaceutical compositions that can be used to treat diseases including immunological conditions and hematological malignancies.

95. 发明公开

EP1993586A4 METHOD FOR TREATING PERIPHERAL ARTERIAL DISEASE WITH ZINC FINGER PROTEINS 审中-公开
标题翻译：法外周动脉疾病与锌指蛋白的治疗
公开(公告)号：EP1993586A4
公开(公告)日：2009-10-21
申请号：EP07763713
申请日：2007-02-09
申请人： SANGAMO BIOSCIENCES INC
发明人： TREMBLE PATRICE , ROKOVICH JOE , ANNEX BRIAN
IPC分类号： A61K38/16 , A61K31/7105
CPC分类号： A61K38/16
摘要： Disclosed is a method for the administration of zinc finger proteins (ZFPs) or nucleic acids that encode such ZFPs for treating peripheral arterial disease, particularly by the repeated administration at regular intervals if such ZFPs or nucleic acids that encode such ZFPs.

96. 发明公开

EP2027262A2 VARIANT FOKI CLEAVAGE HALF-DOMAINS 有权
标题翻译： VARIANT的FokⅠ-分裂半部DOMAINS
公开(公告)号：EP2027262A2
公开(公告)日：2009-02-25
申请号：EP07795299.2
申请日：2007-05-23
申请人： Sangamo BioSciences, Inc.
发明人： MILLER, Jeffrey C.
IPC分类号： C12N9/22 , C12N15/55 , C12N15/62
CPC分类号： C12N9/22
摘要： Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence.

97. 发明公开

EP2018424A2 METHODS AND COMPOSITIONS FOR INACTIVATION OF DIHYDROFOLATE REDUCTASE 审中-公开
标题翻译：方法和组合物用于禁用二氢叶酸还原酶
公开(公告)号：EP2018424A2
公开(公告)日：2009-01-28
申请号：EP07777118.6
申请日：2007-05-17
申请人： Sangamo BioSciences, Inc.
发明人： COLLINGWOOD, Trevor
IPC分类号： C12N9/22 , C12N15/62 , C07K14/00 , C12N15/87
CPC分类号： C12N15/62 , A61K48/00 , C12N9/22
摘要： Disclosed herein are methods and compositions for inactivating dihydrofolate reductase genes, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

98. 发明公开

EP1546400A4 HIGH-THROUGHPUT SCREEN FOR MODULATORS OF CHROMATIN MODIFYING ACTIVITY 审中-公开
标题翻译：高通量筛选调节剂染色质修饰活动
公开(公告)号：EP1546400A4
公开(公告)日：2007-09-19
申请号：EP03793288
申请日：2003-08-22
申请人： SANGAMO BIOSCIENCES INC
发明人： SNOWDEN ANDREW
IPC分类号： C12N9/10 , C12Q1/68 , C07H21/04 , C12N15/00 , C12N15/09
CPC分类号： C12Q1/6897 , C07K2319/00 , C12N9/1007
摘要： Disclosed herein are compositions and method useful in screening a compound for its ability to modulate the activity of a chromatin modifying enzyme. These include fusions between a targeted DNA-binding domain and a chromatin modifying enzyme (or functional fragment thereof), polynucleotides encoding such fusions, and methods for their use.

99. 发明公开

EP1720995A2 METHODS AND COMPOSTIONS FOR TARGETED CLEAVAGE AND RECOMBINATION 有权
标题翻译：方法和组合物靶向剪切和重组
公开(公告)号：EP1720995A2
公开(公告)日：2006-11-15
申请号：EP05756438.7
申请日：2005-02-03
申请人： Sangamo BioSciences, Inc.
发明人： MILLER, Jeffrey C. , ZHANG, Lei
IPC分类号： C12P19/34 , C12N9/22
CPC分类号： A61K35/12 , C07K14/43595 , C07K14/4717 , C07K14/715 , C07K2319/00 , C07K2319/09 , C07K2319/81 , C12N9/14 , C12N9/22 , C12N15/62 , C12N15/85 , C12N2800/80
摘要： Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, as well as polynucleotides encoding same. Fusion proteins comprising cleavage half-domains are used in pairs, to reconstitute a functional cleavage domain. In these fusion proteins, the zinc finger domain can be N-terminal to the cleavage half-domain, or the cleavage half-domain can be N-terminal to the zinc finger domain. The availability of fusion endonucleases having these different polarities allows targeting (and thereby binding) of zinc finger endonucleases either to opposite strands of the DNA target or to the same strand of the DNA target, thereby increasing the number of possible sequences which can be targeted and cleaved by the fusion proteins.

100. 发明公开

EP1678315A4 ENGINEERED ZINC FINGER PROTEINS FOR REGULATION OF GENE EXPRESSION 有权
标题翻译： Sa基因生产的锌指蛋白用于基因表达调控
公开(公告)号：EP1678315A4
公开(公告)日：2006-11-08
申请号：EP04784464
申请日：2004-09-17
申请人： SANGAMO BIOSCIENCES INC
发明人： MOORE MICHAEL , CHOO YEN , KLUG AARON
IPC分类号： C12P21/06 , C07H17/00 , C07K14/00 , C07K14/47 , C12N20060101
CPC分类号： C07K14/4702
摘要： Disclosed herein are methods and compositions for modulation of gene expression, with single-gene specificity, in a human sized genome.

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