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1. 发明授权

EP2454600B1 SPIN COLUMN SYSTEM AND METHODS 有权
公开(公告)号：EP2454600B1
公开(公告)日：2018-11-28
申请号：EP10737411.8
申请日：2010-07-14
申请人： Agilent Technologies, Inc.
发明人： SAKOWSKI, Robert , FULTON, Scott
IPC分类号： G01N35/10 , G01N1/40 , B01D15/08 , B01L3/00 , B01L7/00 , B01L9/00 , G01N30/46 , G01N30/60
CPC分类号： G01N35/1074 , B01L3/50255 , B01L7/00 , B01L9/543 , B01L2300/0829 , B01L2400/0409 , G01N30/467 , G01N30/6043 , G01N30/6091 , G01N2035/1053 , Y10T436/255
摘要： The present invention provides a low bed-volume spin column system and methods of use. The system includes various combinations of spin columns with packed beds, a rack for holding the spin columns, a receiver plate that attaches to the rack and that has wells in registration with the spin columns held in the rack, a lid that attaches to the receiver plate and seals the spin columns, and an incubator block configured for accepting the wells of the receiver plate and for incubating the packed bed. The lid, spin columns, rack, receiver plate, and incubator block are preferably capable of being assembled in a nested configuration. In preferred versions of the invention, the rack is compatible with and attachable to conventional 96-well microplates. Further provided are methods of using the spin column system that include quantitatively purifying and analyzing an analyte, removing and preventing air entrainment within the packed bed, and incubating the packed bed while preventing drying thereof.

2. 发明授权

EP2965073B1 TRANSLOCATION OF A POLYMER THROUGH A NANOPORE 有权
公开(公告)号：EP2965073B1
公开(公告)日：2018-10-31
申请号：EP14759439.4
申请日：2014-03-05
申请人： Arizona Board Of Regents On Behalf Of Arizona State University
发明人： ZHANG, Peiming , LINDSAY, Stuart
IPC分类号： G01N27/26 , G01N33/68 , G06F19/22 , G01N33/543
CPC分类号： G01N33/48721 , C12Q1/37 , G01N15/12 , G01N27/44704 , G01N27/44747 , G01N27/44756 , G01N33/5438 , G01N33/6803 , G01N33/6848 , G01N2333/952 , G01N2570/00 , Y10T436/255
摘要： Embodiments of the present disclosure are directed to methods, systems and devices, for analyzing the molecules. For example, in some embodiments, a system is provided which includes a first volume of conducting fluid, a second volume of conducting fluid, an orifice in communication with said first and second volumes of fluid, and means for applying an electric potential difference between said first and second volumes of fluid. In some such embodiments, a conjugate product is provided which comprises charged polymers each having attached thereto at least one first molecule for analysis, where the product carries a predetermined charge greater than the charge on the first molecule, and upon dissolving a product in the first volume of fluid, the product is directed into the orifice.

3. 发明授权

EP2144824B1 Vessel and method for containing fluids 失效转让
公开(公告)号：EP2144824B1
公开(公告)日：2018-08-15
申请号：EP08745420.3
申请日：2008-04-10
申请人： Waters Technologies Corporation
发明人： GILAR, Martin , BENEVIDES, Christopher, C. , FOURNIER, Jennifer, H.
IPC分类号： B65D51/18
CPC分类号： B01L3/50825 , B01L3/5021 , B01L2200/026 , B01L2200/142 , B01L2300/0858 , Y10T137/0318 , Y10T137/7869 , Y10T137/86187 , Y10T436/25375 , Y10T436/255
摘要： A device for limiting sample evaporation. The device has a vessel body having a body exterior surface and at least one body interior surface. The at least one body interior surface has one or more walls defining a first chamber, a second chamber and a conduit means. The first chamber has a first chamber opening for receiving at least one of the groups selected from a sample and a sample dispensing device. The first chamber opening defines the border of the body exterior surface and the body interior surface. The conduit means is in communication with the first chamber and the second chamber for receiving at least one of the group consisting of sample from said first chamber, a sample from a sample dispensing device and a sample dispensing device passing through or into the conduit means for placing the sample in the conduit means or into the second chamber. The second chamber is in communication with the conduit means for receiving and containing the sample from at least one of the group selected from the sample dispensing device and conduit means. The conduit means is arranged such that the sample in the second chamber is substantially isolated from the atmosphere to limit sample evaporation in the second chamber as the sample is contained therein.

4. 发明授权

EP2136196B1 METHOD OF EXTRACTING POLYCHLORINATED BIPHENYLS 有权转让
公开(公告)号：EP2136196B1
公开(公告)日：2018-06-27
申请号：EP08739128.0
申请日：2008-03-21
申请人： Ehime University , Miura Co., Ltd.
发明人： HONDA, Katsuhisa , TAKAHASHI, Tomofumi
IPC分类号： G01N30/46 , G01N30/60 , B01D15/00 , G01N30/88 , B01J20/32 , G01N33/28 , B01J20/10
CPC分类号： G01N33/2835 , B01D15/00 , B01J20/103 , B01J20/3204 , B01J20/3236 , G01N30/462 , G01N30/6039 , G01N30/88 , G01N2030/8845 , G01N2030/8854 , Y10T436/25375 , Y10T436/255
摘要： A column 1 is formed which includes a first column 10 in which an upper layer 14 made of sulfuric acid silica gel and a lower layer 15 made of silver nitrate silica gel are stacked and packed and a second column 20 packed with an alumina layer 23 and connected detachably to the lower end part of the first column 10. An oily liquid containing polychlorinated biphenyls, such as an electric insulating oil, is added to the upper layer 14 and then kept for a predetermined time in a state heated to at least 35°C, An aliphatic hydrocarbon solvent such as n-hexane is supplied to the upper layer 14 having been cooled to ordinary temperature. As a result, polychlorinated biphenyls captured by the first column 10 are dissolved in the aliphatic hydrocarbon solvent and then flows to the second column 20. The polychlorinated biphenyls are captured by that part of the alumina layer 23 which is located near the inlet of the second column 20. The second column 20 is then detached from the first column 10. A hydrophobic solvent such as toluene is supplied to and passed through the second column 20 in a direction opposite to the direction in which n-hexane was passed. Thus, an extract containing the polychlorinated biphenyls dissolved in a small amount of the hydrophobic solvent is obtained. The time taken to obtain the polychlorinated-biphenyls extract after addition of the oily liquid to the first column 10 is generally as short as about 2 to 10 hours,

5. 发明授权

EP2258845B1 Isolation and purification of nucleic acids 有权
标题翻译：核酸的分离和纯化
公开(公告)号：EP2258845B1
公开(公告)日：2017-08-23
申请号：EP10177127.7
申请日：2002-09-12
申请人： Promega Corporation
发明人： Nargessi, R.D. , Pourfarzaneh, Matt
IPC分类号： C12N15/10
CPC分类号： C12N1/08 , C12N15/1006 , C12N15/1017 , Y10T436/25375 , Y10T436/255

6. 发明授权

EP2447699B1 Apparatus for punching and solid phase extraction of dried biological fluid spot and related methods 有权
标题翻译：对于冲压和干燥生物流体的污渍和相关方法的固相萃取的装置
公开(公告)号：EP2447699B1
公开(公告)日：2017-05-24
申请号：EP11182834.9
申请日：2011-09-27
申请人： Agilent Technologies, Inc.
发明人： Grenz, Robert Lee , Hudson, William C.
IPC分类号： G01N1/40
CPC分类号： G01N1/286 , B01L3/0275 , B01L3/5023 , B01L2300/0672 , B01L2300/0681 , G01N1/405 , G01N2001/288 , Y10T436/255

7. 发明授权

EP2528443B1 QUANTITATIVE ANALYSIS OF VITAMIN D3, VITAMIN D2, AND METABOLITES THEREOF 有权转让
标题翻译：定量分析维生素D3，维生素D 2代谢代谢
公开(公告)号：EP2528443B1
公开(公告)日：2017-01-11
申请号：EP11735362.3
申请日：2011-01-25
申请人： DH Technologies Development Pte. Ltd.
发明人： DEY, Subhakar , PILLAI, Sasi , WILLIAMSON, Brian , PURKAYASTHA, Subhasish
IPC分类号： A01N45/00 , A61K31/59 , A61K31/41 , G01N33/82 , G01N27/62 , G01N33/543
CPC分类号： G01N33/82 , Y10T436/20 , Y10T436/200833 , Y10T436/203332 , Y10T436/24 , Y10T436/25 , Y10T436/25375 , Y10T436/255 , Y10T436/25875

8. 发明授权

EP2175999B1 RECEPTACLES FOR USE IN PERFORMING PROCESSES 有权
标题翻译： BEHÄLTERZUR DURCHFHHRUNG VON PROZESSEN
公开(公告)号：EP2175999B1
公开(公告)日：2017-01-04
申请号：EP08768656.4
申请日：2008-06-20
申请人： Gen-Probe Incorporated , Qualigen, Inc.
发明人： BREIDENTHAL, Scott S. , FAN, Sara H. , KACIAN, Daniel L. , LEE, Richard S. , NELSON, Norman C. , SCOTT, Matthew J. , TAYLOR, Jason A.
IPC分类号： B01L3/00 , G01N35/00 , B01F11/00
CPC分类号： B01F11/00 , B01F3/1271 , B01F11/0045 , B01F11/0071 , B01F13/0059 , B01F15/00214 , B01F2215/0037 , B01L3/502 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/0621 , B01L2200/0647 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2300/1822 , B01L2300/1883 , B01L2400/0457 , B01L2400/0478 , B01L2400/0481 , B01L2400/0655 , B01L2400/0677 , C12N15/1006 , C12Q1/6806 , C12Q1/6844 , G01N1/44 , G01N21/01 , G01N21/6428 , G01N21/645 , G01N35/0098 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6463 , G01N2035/00544 , G01N2201/0627 , Y10T137/0391 , Y10T436/143333 , Y10T436/25 , Y10T436/255
摘要： A detector for detecting an optical signal from a sample that may be indicative of the presence, amount, or state of one or more analytes in the sample, the detector comprising: one or more excitation channels, each disposed within one of the conduits of the housing and adapted to direct an excitation signal of a prescribed excitation wavelength or range of excitation wavelengths toward the sample, and each excitation channel comprising: a light emitting element adapted to emit excitation light; and excitation optical elements defining an excitation optical path having an excitation optic axis, the excitation optical elements comprising a lens and an excitation filter constructed and arranged to transmit only light emitted by the light emitting element having the prescribed excitation wavelength or range of excitation wavelengths toward the sample, one or more emissions channels, each disposed within one of the conduits of the housing and adapted to receive an emission signal from the sample and detect an emission signal having a prescribed emission wavelength or range of emission wavelengths, and each emission channel comprising: emission optical elements defining an emission optical path having an emission optic axis, the emission optical elements comprising a lens and an emission filter constructed and arranged to transmit only light emitted by the sample having the prescribed emission wavelength or range of emission wavelengths; and a light-detecting element adapted to detect light transmitted by the emission optical elements and to convert the detected light to an electronic signal indicative of at least one of the presence and strength of the detected light, a housing, wherein each of the excitation and emission channels is disposed within a different conduit defined within the housing; wherein the excitation and emission optic axes are parallel to one another throughout their extents; and an optic lens constructed and arranged with respect to the excitation and emission channels to (1) direct excitation light transmitted by each excitation channel and impinging on a different portion of the optic lens at a prescribed location and (2) receive emission signals emitted by the sample at the prescribed location and to direct at least a portion of the received emission signals into each emission channel.
摘要翻译：一种用于检测来自样品的光信号的检测器，其可以指示样品中一种或多种分析物的存在，量或状态，所述检测器包括：一个或多个激发通道，每个激发通道设置在所述样品的一个管道内并且适于将规定的激发波长或激发波长范围的激励信号引导到样品，并且每个激励通道包括：适于发射激发光的发光元件; 激励光学元件限定具有激发光轴的激发光路，所述激发光学元件包括透镜和激励滤光器，所述激发光学元件被构造和布置成仅发射具有规定的激发波长或激发波长范围的发光元件发射的光，朝向所述样品，一个或多个排放通道，每个排放通道均设置在所述壳体的一个管道内，并且适于接收来自所述样品的发射信号，并且检测具有规定的发射波长或发射波长范围的发射信号，并且每个发射通道包括限定具有发射光轴的发射光路的发射光学元件，所述发射光学元件包括透镜和发射滤光器，所述发射滤光器构造和布置成仅透射由具有规定发射波长或发射波长范围的样品发射的光; 以及光检测元件，其适于检测由所述发射光学元件发射的光并且将检测到的光转换为指示所检测到的光的存在和强度中的至少一个的电子信号，壳体，其中所述激发和排放通道设置在限定在壳体内的不同导管内; 其中所述激发和发射光轴在其整个范围内彼此平行; 以及相对于激发和发射通道构造和布置的光学透镜，以（1）直接由每个激发通道传输的激发光并且在预定位置处照射在光学透镜的不同部分上，以及（2）接收由在规定位置处的采样并且将接收到的发射信号的至少一部分引导到每个发射通道中。

9. 发明授权

EP2465609B1 Method for mixing the contents of a detection chamber 有权
标题翻译： Verfahren zum Mischen der Inhalte einer Nachweiskammer
公开(公告)号：EP2465609B1
公开(公告)日：2016-12-28
申请号：EP12159759.5
申请日：2008-06-20
申请人： Gen-Probe Incorporated , Qualigen, Inc.
发明人： Breidenthal, Scott , Fan, Sara , Lee, Richard , Nelson, Norman , Scott, Matthew , Taylor, Jason
IPC分类号： B01L3/00 , G01N35/00 , B01F11/00
CPC分类号： B01F11/00 , B01F3/1271 , B01F11/0045 , B01F11/0071 , B01F13/0059 , B01F15/00214 , B01F2215/0037 , B01L3/502 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/0621 , B01L2200/0647 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2300/1822 , B01L2300/1883 , B01L2400/0457 , B01L2400/0478 , B01L2400/0481 , B01L2400/0655 , B01L2400/0677 , C12N15/1006 , C12Q1/6806 , C12Q1/6844 , G01N1/44 , G01N21/01 , G01N21/6428 , G01N21/645 , G01N35/0098 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6463 , G01N2035/00544 , G01N2201/0627 , Y10T137/0391 , Y10T436/143333 , Y10T436/25 , Y10T436/255
摘要： A detector for detecting an optical signal from a sample that may be indicative of the presence, amount, or state of one or more analytes in the sample, the detector comprising: one or more excitation channels, each disposed within one of the conduits of the housing and adapted to direct an excitation signal of a prescribed excitation wavelength or range of excitation wavelengths toward the sample, and each excitation channel comprising: a light emitting element adapted to emit excitation light; and excitation optical elements defining an excitation optical path having an excitation optic axis, the excitation optical elements comprising a lens and an excitation filter constructed and arranged to transmit only light emitted by the light emitting element having the prescribed excitation wavelength or range of excitation wavelengths toward the sample, one or more emissions channels, each disposed within one of the conduits of the housing and adapted to receive an emission signal from the sample and detect an emission signal having a prescribed emission wavelength or range of emission wavelengths, and each emission channel comprising: emission optical elements defining an emission optical path having an emission optic axis, the emission optical elements comprising a lens and an emission filter constructed and arranged to transmit only light emitted by the sample having the prescribed emission wavelength or range of emission wavelengths; and a light-detecting element adapted to detect light transmitted by the emission optical elements and to convert the detected light to an electronic signal indicative of at least one of the presence and strength of the detected light, a housing, wherein each of the excitation and emission channels is disposed within a different conduit defined within the housing; wherein the excitation and emission optic axes are parallel to one another throughout their extents; and an optic lens constructed and arranged with respect to the excitation and emission channels to (1) direct excitation light transmitted by each excitation channel and impinging on a different portion of the optic lens at a prescribed location and (2) receive emission signals emitted by the sample at the prescribed location and to direct at least a portion of the received emission signals into each emission channel.
摘要翻译：一种用于检测来自样品的光信号的检测器，其可以指示样品中一种或多种分析物的存在，量或状态，所述检测器包括：一个或多个激发通道，每个激发通道设置在所述样品的一个管道内并且适于将规定的激发波长或激发波长范围的激励信号引导到样品，并且每个激励通道包括：适于发射激发光的发光元件; 激励光学元件限定具有激发光轴的激发光路，所述激发光学元件包括透镜和激励滤光器，所述激发光学元件被构造和布置成仅发射具有规定的激发波长或激发波长范围的发光元件发射的光，朝向所述样品，一个或多个排放通道，每个排放通道均设置在所述壳体的一个管道内，并且适于接收来自所述样品的发射信号，并且检测具有规定的发射波长或发射波长范围的发射信号，并且每个发射通道包括限定具有发射光轴的发射光路的发射光学元件，所述发射光学元件包括透镜和发射滤光器，所述发射滤光器构造和布置成仅透射由具有规定发射波长或发射波长范围的样品发射的光; 以及光检测元件，其适于检测由发射光学元件发射的光并将检测到的光转换为指示检测到的光的存在和强度中的至少一个的电子信号，壳体，其中每个激发和排放通道设置在限定在壳体内的不同导管内; 其中所述激发和发射光轴在其整个范围内彼此平行; 以及相对于激发和发射通道构造和布置的光学透镜，以（1）直接由每个激发通道传输的激发光并且在预定位置处照射在光学透镜的不同部分上，以及（2）接收由在规定位置处的采样并且将接收到的发射信号的至少一部分引导到每个发射通道中。

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