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    • 9. 发明公开
    • Method and Apparatus for In-Vitro-Analysis of Biological Cells and/or Microorganisms
    • 用于体外生物细胞和/或微生物分析的方法和使用的装置的
    • EP2299259A1
    • 2011-03-23
    • EP09170329.8
    • 2009-09-15
    • TETEC Tissue Engineering Technologies AG
    • Joseph, Heinz WalterLembert, Nicolas
    • G01N21/35G01N33/50G01N33/68
    • G01N21/35G01N2021/3196G01N2021/3595G01N2201/129
    • The invention refers to a method for in-vitro analysis of biological cells and/or microorganisms characteristics selected from the group consisting of degree of differentiation, cell types, donor individuals, culture conditions, purity, lack of natural characteristic, additional characteristic in comparison to natural characteristics, comprising the steps of: (a) projecting infrared radiation on a sample of biological cells and/ or microorganisms, said radiation including a selected number of wavelengths; (b) recording the spectral characteristics after interaction with said sample; (c) deriving a Fourier transform infrared spectrum (FT-IR) from the collected spectral characteristics; (d) generating the first or multiple derivative transformation of the FT-IR spectrum suitable for analysis in a computer; (e) comparing said derivative with the derivative of a reference FT-IR spectrum determined earlier; (f) identifying deviations of said derivative from said reference derivative; (g) providing an analysis of said characteristics based on the presence of said deviations. In addition, the invention relates to an apparatus for carrying out this method as well as to the use of this method and said apparatus in the field of transplantation/graft and tissue engineering, clinical medicine, pharmaceutical industry, quality control and/or drug safety.
    • 本发明涉及一种用于体外分析从组相比,分化,细胞类型,供体值得个体,培养条件,纯度,缺乏自然特性,附加特性的程度由......组成选定的生物细胞和/或微生物特性的 自然特性,其包括以下步骤:(a)关于生物细胞和/或微生物的样品投射红外辐射,所述辐射包括波长的选定数目; (B)记录与所述样本相互作用之后的光谱特性; (C)导出傅立叶从所收集的光谱特性变换红外光谱(FT-IR); (D)产生适于在计算机分析的FT-IR光谱的第一个或多个衍生物转化; (E)比较所述衍生物与开采此前参考FT-IR光谱确定性的衍生物; (F)确定从参考所述衍生物所述衍生物的偏差; (G)提供关于基于所述偏离存在所述特征分析。 此外,本发明涉及到用于执行这种方法以及于使用在移植/移植物和组织工程,临床医学,制药工业,质量控制和/或药物安全领域本方法和所述设备的 ,