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1. 发明授权

EP2110438B1 METHOD FOR INCREASING THE CONTENT OF DOCOSAHEXAENOIC ACID IN MATERIAL CONTAINING OIL-AND-FAT OR OIL-AND-FAT 有权
标题翻译：方法来增加碳六烯酸的比例在油脂含物质或装在油和油脂
公开(公告)号：EP2110438B1
公开(公告)日：2014-06-25
申请号：EP08703933.5
申请日：2008-01-25
申请人： The University of Miyazaki , NIPPON SUISAN KAISHA, LTD.
发明人： HAYASHI, Masahiro , NAKAJIMA, Sanae , NAGANO, Naoki , TAOKA, Yosuke
IPC分类号： C12P7/64 , C11C3/00 , C12N1/14
CPC分类号： C12P7/6427 , C11C3/00 , C12N1/14 , C12P7/6472

2. 发明公开

EP2110438A1 METHOD FOR INCREASING THE CONTENT OF DOCOSAHEXAENOIC ACID IN MATERIAL CONTAINING OIL-AND-FAT OR OIL-AND-FAT 有权
标题翻译：方法来增加碳六烯酸的比例在油脂含物质或装在油和油脂
公开(公告)号：EP2110438A1
公开(公告)日：2009-10-21
申请号：EP08703933.5
申请日：2008-01-25
申请人： The University of Miyazaki , NIPPON SUISAN KAISHA, LTD.
发明人： HAYASHI, Masahiro , NAKAJIMA, Sanae , NAGANO, Naoki , TAOKA, Yosuke
IPC分类号： C12P7/64 , C11C3/00 , C12N1/14
CPC分类号： C12P7/6427 , C11C3/00 , C12N1/14 , C12P7/6472
摘要： [PROBLEMS] To provide a fat-and-oil in which the content of docosahexaenoic acid is increased.
[MEANS FOR SOLVING PROBLEMS] A process for producing highly unsaturated fatty acids comprising culturing a stramenopile capable of producing highly unsaturated fatty acids in a culture medium containing an inhibitor for fatty acid desaturases; fats-and-oils in which the content of highly unsaturated fatty acids, particularly docosahexaenoic acid produced by the relevant method, is increased. A method for enhancing the productivity of highly unsaturated fatty acids in stramenopiles, comprising culturing a stramenopile in a culture medium containing an inhibitor for fatty acid desaturases; stramenopiles having the enhanced productivity of highly unsaturated fatty acids, generated by the relevant method. Particularly, the process for producing highly unsaturated fatty acids, the method for enhancing the productivity of highly unsaturated fatty acids, and the stramenopile having the enhanced productivity of highly unsaturated fatty acids, wherein the stramenopile is a microorganism classified into Labyrinthulea.
摘要翻译： [问题]为了提供一种脂肪和油中的哪十二碳六烯酸的含量增加。 [解决问题的手段]一种制造高度不饱和脂肪酸，包括培养能够产生在培养基中在抑制剂脂肪酸去饱和酶含有高度不饱和脂肪酸的一个不等鞭毛类过程; 脂肪 - 和 - 油在其中的高度不饱和脂肪酸，由相关方法生产的特别十二碳六烯酸含量增大。一种用于增强的高度不饱和脂肪酸在原生藻菌生产率，包括培养在培养基中，在抑制剂脂肪酸去饱和酶包含一个不等鞭毛类方法; 具有的高度不饱和脂肪酸的生产率提高，由相关方法生成原生藻菌。特别地，生产高不饱和脂肪酸，用于增强的高度不饱和脂肪酸的生产率的方法，以及具有的高度不饱和脂肪酸的提高生产率的不等鞭毛类，worin的不等鞭毛类的过程中是分为Labyrinthulea的微生物。

3. 发明公开

EP2405018A1 METHOD FOR DETERMINING FATTY ACID SYNTHESIS PATHWAY OF MICROORGANISM, AND PCR PRIMER SET FOR USE IN THE METHOD 审中-公开
标题翻译：程序对测定微生物和PCR引物的脂肪酸合成PATH用于该方法
公开(公告)号：EP2405018A1
公开(公告)日：2012-01-11
申请号：EP10748811.6
申请日：2010-03-04
申请人： University of Miyazaki , Nippon Suisan Kaisha, Ltd. , Kyushu University, National University Corporation
发明人： NAGANO, Naoki , HAYASHI, Masahiro , SAKAGUCHI, Keishi , ITO, Makoto , TAOKA, Yosuke , OKITA, Yuji
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/689
摘要： Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for Δ4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.
摘要翻译：公开了用于确定性的采矿方法的微生物的脂肪酸合成途径。具体地说游离缺失盘是由制造简并引物用于脂肪酸合成有关的酶基因和确定性采矿在基因组中基因的存在或不存在的脂肪酸合成相关的基因的表征确定性采矿的方法的微生物的脂肪酸合成途径，所有使用简并引物微生物提取。对于简并引物用于C20的序列延伸酶的基因，所有这些是脂肪酸合成相关酶的基因，是ATHGARTWYTKBRTITTYGTICA（SEQ ID NO：1）和TARTRISWRTACATIADIAMRTG（SEQ ID NO：2），以及用于简并引物的序列为“4 去饱和酶基因是GGNCAYCAYCMITAYACNAA（SEQ ID NO：3）和TCDATYTGRTGIBWIARNCC（SEQ ID NO：4）。微生物是一个属于类Labyrinthulea。所以特别游离缺失盘是用于在确定方法中使用的PCR引物组。该方法是作为用于筛选能够合成脂肪酸的微生物的方法是有用的。

4. 发明公开

EP2481799A9 METHOD FOR TRANSFORMING STRAMENOPILE 审中-公开
标题翻译：转换STRAMENOPILE的方法
公开(公告)号：EP2481799A9
公开(公告)日：2013-02-13
申请号：EP10818879.8
申请日：2010-09-24
申请人： Kyushu University, National University Corporation , University of Miyazaki , Konan Gakuen , Nippon Suisan Kaisha, Ltd.
发明人： SAKAGUCHI, Keishi , MATSUDA, Takanori , KOBAYASHI, Takumi , ITO, Makoto , NAGANO, Naoki , HAYASHI, Masahiro , HONDA, Daisuke , TAOKA, Yosuke , OKITA, Yuji , IZUMIDA, Hitoshi , SUGIMOTO, Shinichi
IPC分类号： C12N15/09 , C11C3/00 , C12N1/15 , C12N9/02 , C12P7/64 , C12R1/645
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了一种转化方法，由此可以提高生产菌株的有用物质的能力。用于转化原生藻菌的方法包括将外来基因转移到作为迷迭香属的微生物，更具体地讲是迷迭香属，Altornia属，Aplanochytrium属，Schizochytrium属，Aurantiochytrium属，破囊壶菌属，Ulkenia属等的原生藻属。所述外源基因，通过使用电穿孔或基因枪技术转移与抗生素，比色蛋白质和/或脂肪酸去饱和酶（Δ5去饱和酶基因，Δ12去饱和酶基因和/或ω3去饱和酶基因）耐受性有关的基因。

5. 发明公开

EP2966169A1 METHOD FOR TRANSFORMING STRAMENOPILES 审中-公开
标题翻译： VERFAHREN ZUR UMWANDLUNG VON STRAMENOPILEN
公开(公告)号：EP2966169A1
公开(公告)日：2016-01-13
申请号：EP15178199.4
申请日：2010-09-24
申请人： Kyushu University , University of Miyazaki , Konan Gakuen , NIPPON SUISAN KAISHA, LTD.
发明人： SAKAGUCHI, Keishi , MATSUDA, Takanori , KOBAYASHI, Takumi , ITO, Makoto , NAGANO, Naoki , HAYASHI, Masahiro , HONDA, Daisuke , TAOKA, Yosuke , OKITA, Yuji , IZUMIDA, Hitoshi , SUGIMOTO, Shinichi
IPC分类号： C12N15/09 , C11C3/00 , C12N1/15 , C12N9/02 , C12P7/64 , C12R1/645
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula , Altornia , Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了可以提高生产有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的更青霉属，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等的所述外源基因，其是通过使用电穿孔或基因枪技术转移与抗生素，比色蛋白和/或脂肪酸去饱和酶（“5去饱和酶基因”，12去饱和酶基因和/或É3去饱和酶基因）有关的基因。

6. 发明公开

EP2481799A1 METHOD FOR TRANSFORMING STRAMENOPILE 审中-公开
标题翻译： VERFAHREN ZUR TRANSFORMATION VON STRAMENOPILEN
公开(公告)号：EP2481799A1
公开(公告)日：2012-08-01
申请号：EP10818879.8
申请日：2010-09-24
申请人： Kyushu University, National University Corporation , University of Miyazaki , Konan Gakuen , Nippon Suisan Kaisha, Ltd.
发明人： SAKAGUCHI, Keishi , MATSUDA, Takanobu , KOBAYASHI, Takumi , ITO, Makoto , NAGANO, Naoki , HAYASHI, Masahiro , HONDA, Daisuke , TAOKA, Yosuke , OKITA, Yuji , IZUMIDA, Hitoshi , SUGIMOTO, Shinichi
IPC分类号： C12N15/09 , C11C3/00 , C12N1/15 , C12N9/02 , C12P7/64 , C12R1/645
CPC分类号： C12P7/6409 , C11C3/00 , C12N9/0071 , C12N9/0083 , C12N15/79 , C12N15/8201 , C12N15/8247 , C12P7/6427
摘要： Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and/or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and/or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.
摘要翻译：公开了可以提高生产有机物的能力的转化方法。用于转化stramenopile的方法包括将外源基因转移到作为属于Labyrinthula类的微生物的更青霉属，更具体地是属于Labyrinthula，Altornia，Aplanochytrium，Schizochytrium，Aurantiochytrium，Thraustochytrium，Ulkenia等的所述外源基因，其是通过使用电穿孔或基因枪技术转移与抗生素，比色蛋白和/或脂肪酸去饱和酶（“5去饱和酶基因”，12去饱和酶基因和/或É3去饱和酶基因）有关的基因。

7. 发明公开

EP2405018A9 METHOD FOR DETERMINING FATTY ACID SYNTHESIS PATHWAY OF MICROORGANISM, AND PCR PRIMER SET FOR USE IN THE METHOD 审中-公开
标题翻译：程序对测定微生物和PCR引物的脂肪酸合成PATH用于该方法
公开(公告)号：EP2405018A9
公开(公告)日：2012-03-14
申请号：EP10748811.6
申请日：2010-03-04
申请人： University of Miyazaki , Nippon Suisan Kaisha, Ltd. , Kyushu University, National University Corporation
发明人： NAGANO, Naoki , HAYASHI, Masahiro , SAKAGUCHI, Keishi , ITO, Makoto , TAOKA, Yosuke , OKITA, Yuji
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/689
摘要： Disclosed is a method for determining the fatty acid synthesis pathway of a microorganism. Specifically disclosed is a method for determining the fatty acid synthesis pathway of a microorganism, which is characterized by producing degenerate primers for a fatty acid synthesis-related enzyme gene and determining the presence or absence of the fatty acid synthesis-related gene in the genome gene extracted from the microorganism using the degenerate primers. The sequences for degenerate primers for C20-elongase gene, which is a fatty acid synthesis-related enzyme gene, are ATHGARTWYTKBRTITTYGTICA (SEQ ID NO:1) and TARTRISWRTACATIADIAMRTG (SEQ ID NO:2), and the sequences for degenerate primers for Δ4-desaturase gene are GGNCAYCAYCMITAYACNAA (SEQ ID NO:3) and TCDATYTGRTGIBWIARNCC (SEQ ID NO:4). The microorganism is one belonging to the class Labyrinthulea. Also specifically disclosed is a PCR primer set for use in the determination method. The method is useful as a method for screening a microorganism capable of synthesizing a fatty acid.

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