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1. 发明公开

EP3239710A4 METHOD FOR SEPARATING BIOTINYLATED NUCLEIC ACID 审中-公开
公开(公告)号：EP3239710A4
公开(公告)日：2018-05-23
申请号：EP15872907
申请日：2015-12-17
申请人： WAKO PURE CHEM IND LTD
发明人： UKEKAWA RYO
IPC分类号： G01N33/53 , C07K14/375 , C07K17/14 , C12Q1/6806
CPC分类号： G01N33/53 , C07K14/375 , C07K17/14 , C12Q1/68 , C12Q1/6806
摘要： An object of the present invention is to obtain a biotinylated nucleic acid efficiently, by enhancing dissociation efficiency of biotin in the biotinylated nucleic acid and tamavidin 2 in the tamavidin 2-immobilized insoluble carrier, and the present invention relates to "A method for separating a biotinylated nucleic acid, comprising the following steps: (1) a step for contacting a sample containing a biotinylated nucleic acid wherein the biotin is bound to the nucleic acid with a insoluble carrier on which tamavidin is immobilized (a tamavidin-immobilized insoluble carrier) to form a complex of the biotinylated nucleic acid and the tamavidin-immobilized insoluble carrier (step A-1), (2) a step for separating the biotinylated nucleic acid from the complex obtained in the step A-1, in a solution having pH of 7.8 to 9.5, and in the presence of free biotin (step A-2), a method for separating the biotinylated nucleic acid to which the nucleic acid-binding protein is bound, a method for separating the nucleic acid-binding protein, and a kit for separating the nucleic acid.

2. 发明公开

EP3165541A4 GRAFT POLYMER, RESIN COLORED MATTER, METHOD FOR PRODUCING SAME, AND RESIN COMPOSITION CONTAINING RESIN COLORED MATTER 有权转让
公开(公告)号：EP3165541A4
公开(公告)日：2017-10-11
申请号：EP15814405
申请日：2015-07-01
申请人： WAKO PURE CHEM IND LTD , RHOMBIC CORP
发明人： KIMURA TATSUHIKO , TOMIDA MASAYUKI , KIM TAEYEON , SUZUKI YOSUKE , TAKANO MASAHIRO
IPC分类号： C08F8/30 , C08F255/02 , C08L23/00 , C08L101/00 , C09B11/28 , C09B69/10
CPC分类号： C08F255/02 , C08F8/30 , C08L23/00 , C08L101/00 , C09B11/28 , C09B69/10 , C09B69/103 , C09B69/105 , C08F220/30
摘要： Provided are: a graft polymer and a colored resin that are free from color migration or fading caused by bleed-out after coloring, which is a defect of a dye, without impairing vivid color development and transparency of the dye; a method for producing the same; and a resin composition containing the colored resin. The present invention relates to: (1) a graft polymer that has a polyolefin (A) as the main chain, and a polymer (B) polymerized a dye (b) having a radical reactive functional group, in a side chain; (2) the graft polymer which is characterized in that the dye (b) having the radical reactive functional group is a cationic rhodamine derivative, a cationic triarylmethane-type dye derivative or a cationic cyanine-type dye derivative, having an ethylenically unsaturated group; (3) a colored resin consisting of the graft polymer; as well as (4) a method for producing the colored resin, which is characterized by mixing the polyolefin (A), the dye (b) having the radical reactive functional group, and the radical reaction initiator (C), and subjecting a radical reaction of the mixture; and the like.

3. 发明公开

EP3098226A4 BORATE-BASED BASE GENERATOR, AND BASE-REACTIVE COMPOSITION COMPRISING SUCH BASE GENERATOR 有权转让
标题翻译： BORATBASIERTER BASENGENERATOR UND BASENREAKTIVE ZUSAMMENSETZUNG MIT DERARTIGEM BASENGENERATOR
公开(公告)号：EP3098226A4
公开(公告)日：2017-06-21
申请号：EP15741026
申请日：2015-01-22
申请人： WAKO PURE CHEM IND LTD
发明人： SAKAI NOBUHIKO , YANABA KOSUKE
IPC分类号： C07F5/02 , C07C279/26 , C07D233/48 , C07D487/04 , C07F9/22 , C07F9/24 , C09K3/00
CPC分类号： C07F9/22 , C07C229/26 , C07C279/26 , C07C2601/14 , C07D233/50 , C07D487/04 , C07F5/027 , C07F9/065 , C07F9/5337 , C07F9/5721 , C07F9/6584 , C07F9/65848 , G03F7/0045
摘要： An object of the present invention is to provide a compound which is capable of attaining a composition having high storage stability without reacting with a base-reactive compound, even in the case of storage for a long period of time in a mixed state with the base-reactive compound, such as an epoxy-based compound, as well as capable of generating a strong base (guanidines, biguanides, phosphazenes or phosphoniums) by irradiation of light (active energy rays) or heating; a base generator comprising the compound; and a base-reactive composition comprising the base generator and the base-reactive compound. The present invention relates to the compound represented by the general formula (A); the base generator comprising the compound; and the base-reactive composition comprising the base generator and the base-reactive compound. (wherein R 1 represents an alkyl group; an arylalkynyl group which may be substituted with a halogen atom, an alkyl group, an alkoxy group, or an alkylthio group; an alkenyl group; a 2-furylethynyl group; a 2-thiophenylethynyl group; or a 2,6-dithianyl group; R 2 to R 4 each independently represent an alkyl group; an arylalkynyl group which may be substituted with a halogen atom, an alkyl group, an alkoxy group, or an alkylthio group; the aryl group which may be substituted with a halogen atom, an alkyl group, an alkoxy group, or an alkylthio group; a furanyl group; a thienyl group; or an N-alkyl-substituted pyrrolyl group; Z + represents an ammonium cation having a guanidinium group, a biguanidium group or a phosphazenium group, or a phosphonium cation.)
摘要翻译：本发明的目的在于提供一种化合物，即使在与碱反应的混合状态下长时间保存的情况下，也能够得到不与碱反应性化合物反应而具有高储藏稳定性的组合物（活性能量射线）或加热产生强碱（胍，双胍，磷腈或磷鎓）的反应性化合物，如环氧基化合物; 包含该化合物的碱产生剂; 以及包含碱产生剂和碱反应性化合物的碱反应性组合物。本发明涉及由通式（A）表示的化合物; 该碱基生成剂包含该化合物; 以及包含碱产生剂和碱反应性化合物的碱反应性组合物。（其中R 1表示烷基;可以被卤素原子，烷基，烷氧基或烷硫基取代的芳基炔基;链烯基; 2-呋喃基乙炔基; 2-噻吩基乙炔基; 或2,6-二噻烷基; R 2至R 4各自独立地表示烷基;可以被卤素原子，烷基，烷氧基或烷硫基取代的芳基炔基;芳基烷基，烷氧基或烷硫基;呋喃基;噻吩基;或N-烷基取代的吡咯基; Z +表示具有胍基的铵阳离子，可以被卤素原子，烷基，烷氧基或烷硫基取代; 双胍基团或磷腈鎓基团或鏻阳离子）。

4. 发明公开

EP2840136A4 METHOD FOR DETECTING DNA HAVING MICROSATELLITE REGION 有权
标题翻译：用微粒区检测DNA的方法
公开(公告)号：EP2840136A4
公开(公告)日：2015-12-30
申请号：EP13764078
申请日：2013-03-22
申请人： WAKO PURE CHEM IND LTD , KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION
发明人： MATSUKUMA SHOICHI , ISHIKAWA TOMOKAZU , KUROSAWA TATSUO
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6876 , C12Q2600/156

5. 发明公开

EP2799541A4 METHOD FOR DETECTING METHYLATED CYTOSINE BY USING BISULFITE REACTION 有权转让
标题翻译： VERFAHREN ZUM NACHWEIS冯甲基胞苷胞蛋白双歧杆菌
公开(公告)号：EP2799541A4
公开(公告)日：2015-07-29
申请号：EP12858295
申请日：2012-12-10
申请人： WAKO PURE CHEM IND LTD
发明人： HAYASHIDA YUKINOBU , YAMAMOTO NAOYUKI
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： C12Q1/6858 , C12Q1/6806 , C12Q1/6827 , C12Q2523/125 , C12Q2527/125
摘要： It is an object of the present invention to provide a method for converting non-methylated cytosine in a single-stranded DNA into uracil by a novel bisulfite reaction with higher conversion efficiency from non-methylated cytosine into uracil as compared with the conventional bisulfite method, a method for amplifying the single-stranded DNA in which non-methylated cytosine has been converted into uracil, and a method for detecting methylated cytosine in the single-stranded DNA. The present invention relates to (1) a method for converting non-methylated cytosine in a single-stranded DNA into uracil, comprising subjecting the single-stranded DNA to bisulfite reaction under the presence of at least one of compounds shown by the above-described general formula [1] to [8]; (2) a method for amplifying single-stranded DNA in which non-methylated cytosine has been converted into uracil, comprising further subjecting the single-stranded DNA after bisulfite reaction of (1) to PCR reaction; and (3) a method for detecting methylated cytosine in the aforementioned single-stranded DNA, comprising subjecting the single-stranded DNA amplified in (2) to nucleotide sequence analysis,
摘要翻译：本发明的目的是提供一种通过与常规亚硫酸氢盐方法相比，通过新的亚硫酸氢盐反应将非甲基化胞嘧啶转化成尿嘧啶的方法，其具有较高的转化效率，从非甲基化胞嘧啶转化为尿嘧啶，用于扩增其中非甲基化胞嘧啶已经转化成尿嘧啶的单链DNA的方法以及在单链DNA中检测甲基化胞嘧啶的方法。本发明涉及（1）将单链DNA中的非甲基化胞嘧啶转化成尿嘧啶的方法，其包括在至少一种上述化合物存在下使单链DNA进行亚硫酸氢盐反应通式[1]〜[8]; （2）扩增其中非甲基化胞嘧啶已经转化成尿嘧啶的单链DNA的方法，其还包括在（1）的亚硫酸氢盐反应之后对单链DNA进行PCR反应; （3）上述单链DNA中检测甲基化胞嘧啶的方法，包括使（2）中扩增的单链DNA进行核苷酸序列分析，

6. 发明公开

EP2849211A4 ETCHING FLUID AND PRODUCTION METHOD FOR SILICON-BASED SUBSTRATE USING SAME 审中-公开
标题翻译：一种硅基蚀刻基板和方法，以便
公开(公告)号：EP2849211A4
公开(公告)日：2015-07-08
申请号：EP13787729
申请日：2013-05-10
申请人： WAKO PURE CHEM IND LTD
发明人： OHUCHI NAOKO , KAKIZAWA MASAHIKO , TSURUMOTO HIROYUKI , WATANABE TERUMI , KAWARA SHINSHI
IPC分类号： H01L21/308 , C09K13/02 , H01L31/04
CPC分类号： C09K13/02 , H01L31/02363 , H01L31/1804 , Y02E10/50

7. 发明公开

EP2843689A4 CLEANING AGENT FOR SEMICONDUCTOR SUBSTRATES AND METHOD FOR PROCESSING SEMICONDUCTOR SUBSTRATE SURFACE 审中-公开
标题翻译：清洁剂用于半导体基板和方法用于处理半导体衬底的表面
公开(公告)号：EP2843689A4
公开(公告)日：2015-05-13
申请号：EP13780549
申请日：2013-04-26
申请人： WAKO PURE CHEM IND LTD
发明人： KAWADA HIROMI , MIZUTA HIRONORI , MAESAWA TSUNEAKI
IPC分类号： C11D7/32 , C11D7/36 , H01L21/306
CPC分类号： C11D11/0047 , C11D7/3218 , C11D7/36 , H01L21/0206 , H01L21/02068 , H01L21/02074 , H01L21/31055 , H01L21/3212 , H01L21/7684
摘要： The purpose of the present invention is to provide: a cleaning agent for a semiconductor substrate superior in corrosion resistance of a tungsten wiring or a tungsten alloy wiring, and superior in removal property of polishing fines (particle) such as silica or alumina, remaining at surface of the semiconductor substrate, in particular, at surface of a silicon oxide film such as a TEOS film, after a chemical mechanical polishing process; and a method for processing a semiconductor substrate surface. The present invention relates to a cleaning agent for a semiconductor substrate to be used in a post process of a chemical mechanical polishing process of the semiconductor substrate having a tungsten wiring or a tungsten alloy wiring, and a silicon oxide film, comprising (A) a phosphonic acid-based chelating agent, (B) a primary or secondary monoamine having at least one alkyl group or hydroxyalkyl group in a molecule and (C) water, wherein a pH is over 6 and below 7; and a method for processing a semiconductor substrate surface.

8. 发明公开

EP2194385A4 REAGENT CONTAINER 有权转让
标题翻译：试剂
公开(公告)号：EP2194385A4
公开(公告)日：2014-12-24
申请号：EP08833456
申请日：2008-09-24
申请人： WAKO PURE CHEM IND LTD
发明人： DENAWA TATSUYUKI , HAYASHI MASAYOSHI , KUROSAWA TATSUO , SATOMURA MASAHIRO , WATANABE MITSUO
IPC分类号： G01N35/02 , B01L3/00 , G01N35/10
CPC分类号： G01N35/1002 , B01L3/523 , B01L3/527 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0672 , B01L2300/0861 , G01N35/026 , G01N35/1079 , G01N2035/0436
摘要： A reagent container configured not to require a specific strength for a probe, reduce contamination of the probe and the like, allow accurate liquid level detection, and to be opened easily. A reagent container (50) includes a plurality of open holding sections (52a, 52b, 52c, 52d), each holding a reagent and into which a probe is inserted to suck the reagent. An opening (66) of each of the holding sections (52a, 52b, 52c, 52d) are sealed with at least one sheet-like seal member (62). The reagent container (50) includes a container body (52) and a lid (54) having a plurality of hollow piercing sections (64), formed on a lower surface of the lid, protruding downward for piercing the seal member at each opening (66), and openings (86), formed in an upper surface of the lid, communicating with piercing sections (64) respectively to allow the probe to be inserted therethrough or withdrawn therefrom.

9. 发明公开

EP2647693A4 SUBSTRATE CLEANER FOR COPPER WIRING, AND METHOD FOR CLEANING COPPER WIRING SEMICONDUCTOR SUBSTRATE 审中-公开
标题翻译：铜导线用基板洗净和方法清洁铜导体半导体衬底
公开(公告)号：EP2647693A4
公开(公告)日：2014-05-28
申请号：EP11845260
申请日：2011-11-28
申请人： WAKO PURE CHEM IND LTD
发明人： KAWADA HIROMI , SHIRAHATA SATOSHI , MIZUTA HIRONORI , KAKIZAWA MASAHIKO , SHIRAKI KAZUO
IPC分类号： C11D7/32 , C11D7/08 , C11D7/10 , H01L21/304
CPC分类号： C11D7/3245 , C11D7/3209 , C11D7/3218 , C11D11/0047 , H01L21/02074

10. 发明公开

EP2463266A4 PROCESS FOR PRODUCTION OF BIS-QUATERNARY AMMONIUM SALT, AND NOVEL INTERMEDIATE 审中-公开
标题翻译：用于生产BISQUATERNÄREM铵盐和新的中间体
公开(公告)号：EP2463266A4
公开(公告)日：2013-03-13
申请号：EP10806522
申请日：2010-08-05
申请人： WAKO PURE CHEM IND LTD
发明人： OKAMOTO KUNIAKI , WATAHIKI TSUTOMU , SUMINO MOTOSHIGE
IPC分类号： C07C209/20 , C07C211/63 , C07C303/32 , C07C309/73 , C07D213/06 , C07D213/127 , C07D233/64
CPC分类号： C07D213/127 , C07C309/73 , C07D213/06 , C07D233/64 , H01M10/052 , H01M10/0567

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