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1. 发明授权

US06797485B2 Mass spectrometry of colonization factors 失效
标题翻译：定殖因子的质谱
公开(公告)号：US06797485B2
公开(公告)日：2004-09-28
申请号：US09942974
申请日：2001-08-31
申请人： Frederick J. Cassels , Lewis K. Pannell
发明人： Frederick J. Cassels , Lewis K. Pannell
IPC分类号： C12Q124
CPC分类号： C07K14/245 , C12Q1/10 , G01N2333/245 , G01N2560/00
摘要： Disclosed herein are methods for identifying at least one bacterial colonization factor of enterotoxigenic E. coil which comprise the following steps in the following order: 1) obtaining the colonization factor; 2) solubilizing the colonization factor by dissolving the colonization factor in 1,1,1,3,3,3-hexafluoro-2-propanol; 3) adding a solution of volatile acid to the solubilized colonization factor of step 2 to obtain a product; 4) subjecting the product of step 3 to mass spectrometry to determine the mass of the colonization factor; and 5) comparing the mass determined in step 4 with the mass of at least one known colonization factor.
摘要翻译：本文公开了鉴定肠毒素E.线圈的至少一种细菌定植因子的方法，其包括以下顺序的以下步骤：1）获得定植因子; 2）通过将定居因子溶解在1,1,1,3,3,3-六氟-2-丙醇中来溶解定殖因子; 3）将挥发性酸溶液加入到步骤2的溶解的定植因子中以得到产物; 4）使步骤3的产物进行质谱以确定定植因子的质量; 和5）将步骤4中确定的质量与至少一个已知定殖因子的质量进行比较。

2. 发明授权

US06660493B2 Hydrodynamic enhanced dielectrophoretic particle trapping 失效
公开(公告)号：US06660493B2
公开(公告)日：2003-12-09
申请号：US09819410
申请日：2001-03-28
申请人： Robin R. Miles
发明人： Robin R. Miles
IPC分类号： C12Q124
CPC分类号： B01L3/502761 , B01L2200/0636 , B01L2200/0668 , B01L2400/0415 , B01L2400/0424 , B03C5/026
摘要： Hydrodynamic enhanced dielectrophoretic particle trapping carried out by introducing a side stream into the main stream to squeeze the fluid containing particles close to the electrodes producing the dielelectrophoretic forces. The region of most effective or the strongest forces in the manipulating fields of the electrodes producing the dielectrophoretic forces is close to the electrodes, within 100 &mgr;m from the electrodes. The particle trapping arrangement uses a series of electrodes with an AC field placed between pairs of electrodes, which causes trapping of particles along the edges of the electrodes. By forcing an incoming flow stream containing cells and DNA, for example, close to the electrodes using another flow stream improves the efficiency of the DNA trapping.

3. 发明授权

US06514721B2 Air sampler for pathogens and psychrometrics 失效
标题翻译：空气取样器用于病原体和精神病学
公开(公告)号：US06514721B2
公开(公告)日：2003-02-04
申请号：US09898715
申请日：2001-07-03
申请人： Leon Bryan Spurrell
发明人： Leon Bryan Spurrell
IPC分类号： C12Q124
CPC分类号： G01N1/2205 , G01N1/2208 , G01N1/24 , G01N2001/2223 , G01N2001/244 , G01N2001/245 , G01N2015/0261
摘要： An air sampler device and method for collecting airborne pathogens and psychrometric data for room or remote air samples wherein the sample volume is electronically controlled. Particulates in the air are caused to impact the surface of the growth/inhibitor media contained in the pathogen dish thereby depositing pathogenic microorganisms in the media. The growth/inhibitor media may be a solid, liquid, gel, or mixture thereof. After the pathogen dish is incubated, colony forming units are counted for determination of air quality parameters. A chip-based sensor measures psychrometric properties of the air sample.
摘要翻译：用于收集空间样本的空气取样器装置和方法，用于收集空间或空气样本的空气传播病原体和空气湿度数据，其中样品体积是电子控制的。导致空气中的颗粒影响病原体皿中所含的生长/抑制剂介质的表面，从而将介导的病原微生物沉积。生长/抑制剂介质可以是固体，液体，凝胶或其混合物。培养病原菌皿后，计数菌落形成单位以确定空气质量参数。基于芯片的传感器测量空气样品的湿度特性。

4. 发明授权

US06277588B1 Screening of cell populations 失效
标题翻译：筛选细胞群体
公开(公告)号：US06277588B1
公开(公告)日：2001-08-21
申请号：US09090531
申请日：1998-06-04
申请人： Amihay Freeman , George Georgiou
发明人： Amihay Freeman , George Georgiou
IPC分类号： C12Q124
CPC分类号： G01N33/5094 , C12Q1/24
摘要： A method for screening large cell populations for isolation of mutants that express a target activity. The products of the screening method are disclosed. Various activities, including enzymatic activities can be selected.
摘要翻译：筛选大细胞群以分离表达靶标活性的突变体的方法。披露了筛选方法的产品。可以选择各种活动，包括酶活性。

5. 发明授权

US06207406B1 Microbial sampling apparatus and process 失效
标题翻译：微生物取样装置及工艺
公开(公告)号：US06207406B1
公开(公告)日：2001-03-27
申请号：US09418912
申请日：1999-10-15
申请人： Judd R. Wilkins
发明人： Judd R. Wilkins
IPC分类号： C12Q124
CPC分类号： C12Q1/24
摘要： A sampling apparatus and process for obtaining microbial samples from a suspect surface, such as a meat processing facility, animal carcass, and the like, wherein a agar saturated porous plastic foam strip is placed in contact with the suspect surface and thereafter incubated to indicate the presence and quantity of microbial growth on the suspect surface. The porous plastic foam strip is attached to one surface of a double faced adhesive tape with the other tape face being adhesively attached to a spool on a hand roller to permit hand pressure application of the porous plastic sampler to the test surface. For use of the sampler without the roller, one surface of the double face adhesive tape is attached to a plastic strip and the sampler may be hand positioned directly on the suspect test surface. Agar for specific known bacteria may be employed to saturate the porous plastic foam sampler.
摘要翻译：用于从可疑表面（例如肉类加工设备，动物屠体等）获得微生物样品的采样设备和方法，其中将琼脂饱和多孔塑料泡沫条放置成与可疑表面接触，然后孵育以指示可疑表面微生物生长的存在和数量。多孔塑料泡沫条附着在双面胶带的一个表面上，另一个胶带表面粘附在手辊上的卷轴上，以允许将多孔塑料取样器手压施加到测试表面。为了使用没有辊的取样器，双面胶带的一个表面连接到塑料条上，并且取样器可以直接手动定位在可疑的测试表面上。可以使用特定已知细菌的琼脂来饱和多孔塑料泡沫取样器。

6. 发明授权

US06825027B2 Method of production of purified hepatitis a virus particles and vaccine preparation 失效
标题翻译：生产纯化的甲型肝炎病毒颗粒和疫苗制备方法
公开(公告)号：US06825027B2
公开(公告)日：2004-11-30
申请号：US10006205
申请日：2001-12-10
申请人： Christa Tauer , Heidi Meyer , Artur Mitterer , Noel Barrett
发明人： Christa Tauer , Heidi Meyer , Artur Mitterer , Noel Barrett
IPC分类号： C12Q124
CPC分类号： C12N7/00 , A61K39/12 , A61K39/29 , A61K2039/5252 , A61K2039/55505 , A61K2039/57 , C12N2770/32432 , C12N2770/32451 , Y02A50/464 , Y10S435/80 , Y10S435/803 , Y10S435/814 , Y10S435/816 , Y10S435/962
摘要： The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a preparation consisting of purified mature HAV particles in an amount sufficient to induce a protective immune response in a mammal.
摘要翻译：本发明提供从感染的细胞培养物的上清液中纯化甲型肝炎病毒的方法以及纯化的HAV抗原的制备物的制备。本发明还涉及HAV疫苗组合物，其包含由在哺乳动物中诱导保护性免疫应答的量的纯化的成熟HAV颗粒组成的制剂。

7. 发明授权

US06495340B2 Cell carrier grids 失效
标题翻译：细胞载体网格
公开(公告)号：US06495340B2
公开(公告)日：2002-12-17
申请号：US09886205
申请日：2001-06-22
申请人： Tamir Huberman , Yal Schiffenbauer
发明人： Tamir Huberman , Yal Schiffenbauer
IPC分类号： C12Q124
CPC分类号： B01L3/5025 , B01L3/50255 , B01L3/5085 , B01L9/50 , B01L2300/0829 , B01L2400/049 , C12M23/12
摘要： An improved cell carrier grid. The grid is capable of containing and retaining individual living cells in an array discrete locations and includes a body that defines having an ordered array of holes arranged according to an organizational plan such that each of the holes is identifiable. Each of said holes can contain at least a portion of an individual living cell. According to some embodiments of the invention, individual cells contained within said holes reside substantially in a single focal plane so that accuracy of data collected is increased. According to some embodiments of the invention, said body is at least partially coated with a biologically active material. According to some embodiments of the invention, said body is designed and constructed such that said individual cells contained within said holes are recoverable by a recovery device.
摘要翻译：改进的细胞载体网格。网格能够将单个活细胞包含并保持在阵列离散的位置中，并且包括限定具有根据组织计划布置的有序排列的孔的主体，使得每个孔是可识别的。每个所述孔可以包含单个活细胞的至少一部分。根据本发明的一些实施例，包含在所述孔内的各个单元基本上位于单个焦平面中，从而增加收集的数据的精度。根据本发明的一些实施方案，所述主体至少部分地涂覆有生物活性材料。根据本发明的一些实施例，所述主体被设计和构造成使得容纳在所述孔内的所述单个电池可由恢复装置恢复。

8. 发明授权

US06391576B1 Method for isolating a microbe 失效
标题翻译：隔离微生物的方法
公开(公告)号：US06391576B1
公开(公告)日：2002-05-21
申请号：US09517744
申请日：2000-03-03
申请人： Takayasu Tsuchida , Akira Manome , Ryuichiro Kurane
发明人： Takayasu Tsuchida , Akira Manome , Ryuichiro Kurane
IPC分类号： C12Q124
CPC分类号： C12Q1/24
摘要： The present invention provides a method for isolating a microbe whereby a sample of microbe cells is encapsulated in agarose gel particulates, wherein some of the particulates contain a single cell, and the other particulates contain more than one cell; incubating the particulates in nutritional and environmental conditions that enable the microbe contained in the sample solution that can grow on a plate of a plate culture method to grow in the agarose gel particulate; and isolating the particulates having single cells from the group of the particulates having more than one cell.
摘要翻译：本发明提供一种分离微生物的方法，其中将微生物细胞的样品包封在琼脂糖凝胶颗粒中，其中一些微粒包含单个细胞，而其它颗粒含有多于一个细胞; 在营养和环境条件下孵育颗粒物，使得能够在平板培养方法板上生长的样品溶液中包含的微生物在琼脂糖凝胶颗粒中生长; 并从具有多于一个电池的颗粒组中分离出具有单个电池的颗粒。

9. 发明授权

US06277589B1 Method and apparatus for rapid biohydrogen phenotypic screening of microorganisms using a chemochromic sensor 失效
标题翻译：使用化学传感器快速生物氢表型筛选微生物的方法和装置
公开(公告)号：US06277589B1
公开(公告)日：2001-08-21
申请号：US09316172
申请日：1999-05-21
申请人： Michael Seibert , David K. Benson , Timothy Michael Flynn
发明人： Michael Seibert , David K. Benson , Timothy Michael Flynn
IPC分类号： C12Q124
CPC分类号： C12Q1/04 , C12Q2304/40 , G01N31/223 , G01N33/005 , G01N2033/4977
摘要： The invention provides an assay system for identifying a hydrogen-gas-producing organism, including a sensor film having a first layer comprising a transition metal oxide or oxysalt and a second layer comprising hydrogen-dissociative catalyst metal, the first and second layers having an inner and an outer surface wherein the inner surface of the second layer is deposited on the outer surface of the first layer, and a substrate disposed proximally to the outer surface of the second layer, the organism being isolated on the substrate.
摘要翻译：本发明提供了一种用于鉴定产生氢气的生物体的测定系统，包括具有包含过渡金属氧化物或氧化钾的第一层的传感器膜和包含氢解离催化剂金属的第二层，所述第一和第二层具有内部以及外表面，其中所述第二层的内表面沉积在所述第一层的外表面上，以及衬底，其设置在所述第二层的外表面的近侧，所述生物被隔离在所述衬底上。

10. 发明授权

US06723528B1 Inoculation storage assembly and method for use thereof 有权
标题翻译：接种储存组件及其使用方法
公开(公告)号：US06723528B1
公开(公告)日：2004-04-20
申请号：US10280694
申请日：2002-10-28
申请人： Donald H. DeVaughn , Wolfgang Ott
发明人： Donald H. DeVaughn , Wolfgang Ott
IPC分类号： C12Q124
CPC分类号： C12M37/00 , C12M33/04
摘要： An inoculation storage assembly and method of preserving sterility including an inoculation device (20), a storage rack or container (84, 184), and a handling tool (60, 160). The inoculation device (20) being an elongated ember (22) with a gripping section (24), an inoculation section (26), and a seal (25) carried by elongated member (22). The seal (25) preferably having an arcuate surface (19) for sealing engagement against a seal contact surface (162, 262) in a cavity (86, 186) in the storage rack (84, 184). The storage container (84, 184) including an open upper end or opening (88) and a seal contact surface (162, 262) dimensioned to sealingly receive the seal (25), with the inoculation device (20) being mounted in sealed relation to the cavity (86). A handling tool (60, 160) capable of releasable coupling to gripping section (24) of the inoculation device (20) is provided. A method of using the inoculation storage assembly and preserving sterility is also disclosed.
摘要翻译：一种接种储存组件和保存无菌性的方法，包括接种装置（20），储存架或容器（84,184）和处理工具（60,160）。接种装置（20）是具有抓握部分（24）的细长的余烬（22），接种部分（26）和由细长部件（22）承载的密封件。密封件（25）优选地具有用于与存储架（84,184）中的空腔（86,186）中的密封接触表面（162,262）密封接合的弓形表面（19）。包括敞开的上端或开口（88）和密封接触表面（162,262）的储存容器（84,184）的尺寸设置成密封地接收密封件（25），接种装置（20）以密封的关系安装到空腔（86）。提供了能够可释放地联接到接种装置（20）的夹持部分（24）的处理工具（60,160）。还公开了使用接种储存组件和保存无菌的方法。

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