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1. 发明申请

US20040171124A1 Method of resolution and antiviral activity of 1,3-oxathiolane nucleoside enantiomers 失效
标题翻译： 1,3-氧硫杂环戊烷核苷对映异构体的拆分方法及抗病毒活性
公开(公告)号：US20040171124A1
公开(公告)日：2004-09-02
申请号：US10795046
申请日：2004-03-05
申请人： Emory University, Inc.
发明人： Dennis C. Liotta , Raymond F. Schinazi , Woo-Baeg Choi
IPC分类号： C12P019/30
CPC分类号： C07D327/04 , C07D405/04 , C07D411/04 , C07H19/00 , C07H19/06 , C07H19/10
摘要： A process for the resolution of a racemic mixture of nucleoside enantiomers that includes the step of exposing the racemic mixture to an enzyme that preferentially catalyzes a reaction in one of the enantiomers. The nucleoside enantiomer (null)-2-hydroxymethyl-5-(5-fluorocytosin-1-yl)-1,3-oxathiolane is an effective antiviral agent against HIV, HBV, and other viruses replicating in a similar manner.
摘要翻译：一种用于拆分核苷对映异构体外消旋混合物的方法，其包括将外消旋混合物暴露于优先催化其中一种对映异构体中的反应的酶的步骤。核苷对映异构体（ - ） - 2-羟甲基-5-（5-氟胞嘧啶-1-基）-1,3-氧硫杂环戊烷是以类似方式复制的有效的抗HIV，HBV和其他病毒的抗病毒剂。

2. 发明申请

US20040096947A1 Building blocks for the solution phase synthesis of oligonucleotides 审中-公开
公开(公告)号：US20040096947A1
公开(公告)日：2004-05-20
申请号：US10700077
申请日：2003-11-03
发明人： Yogesh S. Sanghvi , Vicente Gotor , Miguel Ferrero , Susana Fernandez , Javier Garcia
IPC分类号： C07H019/00 , C07H019/22 , C12P019/30
CPC分类号： C07H21/04 , C07H19/04 , C12P19/30
摘要： The present invention is directed to methods for the preparation of 3null-O and 5null-O-levulinyl nucleosides from common precursors using an enzymatic approach.

3. 发明申请

US20030082752A1 Enzymatic process for producing GDP-alpha-D-mannose, a GDP mannose pyrophosphorylase and phosphomannomutase suitable for that process, the extraction of the said enzymes, and an enzyme test 审中-公开
标题翻译：用于生产GDP-α-D-甘露糖的酶法，适用于该方法的GDP甘露糖焦磷酸化酶和磷酸三聚谷氨酸酶，所述酶的提取和酶试验
公开(公告)号：US20030082752A1
公开(公告)日：2003-05-01
申请号：US09812864
申请日：2001-03-19
发明人： Jorg Eberhard Ritter , Lothar Elling , Maria-Regina Kula , Stefen Verseck
IPC分类号： C12P019/30 , C12N009/16
CPC分类号： C12N9/1241 , C12N9/90 , C12P19/30 , C12Q1/48
摘要： The invention provides a GDP-mannose-pyrophosphorylase, which is suitable for use in continuous multiple stage processes. The mannose- or mannose-derivative-specific GDP-mannose-prophosphorylase, which can be isolated from microorganisms, has a specific activeity ofnull2U/mg, is prepared.
摘要翻译：本发明提供了一种适用于连续多级方法的GDP-甘露糖 - 焦磷酸化酶。可以从微生物中分离的甘露糖或甘露糖衍生物特异性GDP-甘露糖 - 磷酸化酶具有> = 2U / mg的比活度。

4. 发明申请

US20040259212A1 Process for producing purine nucleotides 审中-公开
标题翻译：嘌呤核苷酸的制备方法
公开(公告)号：US20040259212A1
公开(公告)日：2004-12-23
申请号：US10632906
申请日：2003-08-04
申请人： KYOWA HAKKO KOGYO CO., LTD
发明人： Yutaka Takano , Masato Ikeda , Tatsuro Fujio
IPC分类号： C12P019/30 , C12N001/20
CPC分类号： C12N9/93 , C12N9/1205 , C12N9/16 , C12P19/32
摘要： Purine nucleotides are produced by culturing a microorganism having the ability to produce a precursor of the purine nucleotide and carrying an introduced DNA which can express an enzyme capable of synthesizing the purine nucleotide from the precursor upon induction; allowing the purine nucleotide precursor to accumulate in the culture; inducing the expression of the enzyme; allowing the purine nucleotide formed to accumulate in the culture; and recovering the purine nucleotide. Suitable microorganisms include Corynebacterium ammoniagenes which are induced to express GMP synthetase/XMP aminase and inosine-guanosine kinase for use in producing IMP and GMP, especially from the nucleotide precursor XMP.
摘要翻译：嘌呤核苷酸通过培养具有产生嘌呤核苷酸前体的能力的微生物产生，并携带可诱导诱导后能够从前体合成嘌呤核苷酸的酶的引入的DNA; 使嘌呤核苷酸前体在培养物中积累; 诱导酶的表达; 使形成的嘌呤核苷酸在培养物中积累; 并回收嘌呤核苷酸。合适的微生物包括产生表达GMP合成酶/ XMP氨基酶和肌苷 - 鸟苷激酶用于产生IMP和GMP，特别是来自核苷酸前体XMP的产氨杆菌。

5. 发明申请

US20040229263A1 Los locus from C.jejuni 有权
标题翻译：来自C.jejuni的Los座位
公开(公告)号：US20040229263A1
公开(公告)日：2004-11-18
申请号：US10821604
申请日：2004-04-08
申请人： National Research Council of Canada
发明人： Michel Gilbert , Warren W. Wakarchuk
IPC分类号： C12Q001/68 , C07H021/04 , C12P019/30 , C12N009/10 , C12N015/74
CPC分类号： C12N9/1241 , C12N9/1029 , C12N9/1048 , C12N9/1051 , C12N9/1081 , C12P19/18
摘要： This invention provides prokaryotic glycosyltransferases, including a bifunctional sialyltransferase that has both an null2,3- and an null2,8-activity. A null1,4-GalNAc transferase and a null1,3-galactosyltransferase are also provided by the invention, as are other glycosyltransferases and enzymes involved in synthesis of lipooligosaccharide (LOS). The glycosyltransferases can be obtained from, for example, Campylobacter species, including C. jejuni. In additional embodiments, the invention provides nucleic acids that encode the glycosyltransferases, as well as expression vectors and host cells for expressing the glycosyltransferases.
摘要翻译：本发明提供了原核糖基转移酶，包括具有α2,3-和α2,8-活性的双官能唾液酸转移酶。本发明还提供β1,4-GalNAc转移酶和β1,3-半乳糖基转移酶，以及参与脂寡糖（LOS）合成的其它糖基转移酶和酶。糖基转移酶可以从例如弯曲杆菌属物种获得，包括空肠弯曲杆菌。在另外的实施方案中，本发明提供编码糖基转移酶的核酸，以及用于表达糖基转移酶的表达载体和宿主细胞。

6. 发明申请

US20040175804A1 Process for preparing dideoxyinosine using adenosine deaminase enzyme 审中-公开
标题翻译：使用腺苷脱氨酶制备二脱氧肌苷的方法
公开(公告)号：US20040175804A1
公开(公告)日：2004-09-09
申请号：US10787284
申请日：2004-02-26
发明人： Paul M. Skonezny , Michael Politino , Suo W. Liu , Alfred W. Boyle , Jason G. Chen , Gregory L. Stein , Thomas Franceschini , Wendy L. Anderson
IPC分类号： C07H019/167 , C12P019/30 , A61K031/7076
CPC分类号： C07H19/167 , C12P19/40
摘要： A method of making didanosine (ddI) including the steps of: (a) obtaining an enzyme expressing ddA deaminase activity; (b) immobilizing the enzyme onto an insoluble support; (c) contacting the enzyme with a dideoxyadenosine (ddA) solution of at least about 4% weight volume ddA in water for a time and under conditions to produce a ddI solution; and (d) isolating the ddI from the ddI solution. Optionally, the ddI mother liquor is reused in subsequent runs to improve yield.
摘要翻译：制备去羟肌苷（ddI）的方法包括以下步骤：（a）获得表达ddA脱氨酶活性的酶; （b）将酶固定在不溶性载体上; （c）使酶与至少约4重量％体积的ddA的二脱氧腺苷（ddA）溶液在水中一段时间和在产生ddI溶液的条件下接触; 和（d）将ddI与ddI溶液分离。任选地，ddI母液在随后的运行中重复使用以提高产率。

7. 发明申请

US20030134403A1 Nucleic acids useful for enzymatic conversion of GDP-mannose to GDP-fucose 审中-公开
标题翻译：用于GDP-甘露糖酶转化为GDP-岩藻糖的核酸
公开(公告)号：US20030134403A1
公开(公告)日：2003-07-17
申请号：US10206485
申请日：2002-07-25
申请人： Cytel Corporation
发明人： Eric R. Sjoberg
IPC分类号： C12P019/30 , C07H021/04 , C12N009/02 , C12N015/74 , C12P021/02 , C12N001/21
CPC分类号： C12N9/0008 , C12N9/90 , C12P19/32
摘要： This invention provides methods for practical enzymatic conversion of GDP-mannose to GDP-fucose. These methods are useful for efficient synthesis of reactants used in the synthesis of fucosylated oligosaccharides.
摘要翻译：本发明提供了GDP-甘露糖对GDP-岩藻糖的实际酶转化方法。这些方法对于有效合成用于岩藻糖基化寡糖合成中的反应物是有用的。

8. 发明申请

US20040166568A1 Processes for producing sugar nucleotides and complex carbohydrates 审中-公开
标题翻译：生产糖核苷酸和复合碳水化合物的方法
公开(公告)号：US20040166568A1
公开(公告)日：2004-08-26
申请号：US10781010
申请日：2004-02-18
申请人： KYOWA HAKKO KOGYO CO. LTD.
发明人： Satoshi Koizumi , Hisaji Kawano , Kuniki Kino , Akio Ozaki
IPC分类号： C12P019/30 , C12P019/04 , C12N001/18
CPC分类号： C12P19/305 , C12N9/1051 , C12P19/02 , C12P19/18 , C12P19/26 , C12P19/30
摘要： This invention relates to a process for producing a complex carbohydrate, which comprises: selecting, as enzyme sources, a culture broth of a microorganism capable of producing a sugar nucleotide from a nucleotide precursor and a sugar, or a treated product of the culture broth, and a culture broth of a microorganism or animal cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth; carrying out an enzyme reaction in an aqueous medium containing the enzyme sources, the nucleotide precursor, the sugar and the complex carbohydrate precursor to form and accumulate the complex carbohydrate in the aqueous medium; and recovering the complex carbohydrate from the aqueous medium, and a process for producing a sugar nucleotide, which comprises selecting, as an enzyme source, a culture broth of a microorganism capable of producing a sugar nucleotide from a nucleotide precursor and a sugar, or a treated product of the culture broth; carrying out an enzyme reaction in an aqueous medium containing the enzyme source, the nucleotide precursor and the sugar to form and accumulate the sugar nucleotide in the aqueous medium; and recovering the sugar nucleotide from the aqueous medium.
摘要翻译：本发明涉及一种复合碳水化合物的制备方法，其包括：选择能够从核苷酸前体和糖产生糖核苷酸的微生物的培养液，或培养液的处理产物作为酶源，以及能够从糖核苷酸和复合碳水化合物前体产生复合碳水化合物的微生物或动物细胞的培养液，或培养液的处理产物; 在含有酶源，核苷酸前体，糖和复合碳水化合物前体的水性介质中进行酶反应，以在水性介质中形成并积聚复合碳水化合物; 和从水性介质中回收复合碳水化合物，以及制备糖核苷酸的方法，其包括从核苷酸前体和糖中选择能够产生糖核苷酸的微生物的培养液作为酶源，或培养液的处理产物; 在含有酶源，核苷酸前体和糖的水性介质中进行酶反应，以在水性介质中形成并积聚糖核苷酸; 并从水介质中回收糖核苷酸。

9. 发明申请

US20040162424A1 Method of producing derivatives of lactosamine 审中-公开
标题翻译：生产乳糖衍生物的方法
公开(公告)号：US20040162424A1
公开(公告)日：2004-08-19
申请号：US10679194
申请日：2003-10-03
发明人： Kurt Nilsson
IPC分类号： C12P019/30 , C07H005/06
CPC分类号： C12P19/26 , C07H3/04
摘要： Disclosed is a method of producing a compound with null1-4 linkage which contains the lactosamine structure involving reacting at least one donor substance GalnullOR where R is an organic group, and at least one acceptor substance which is a glucopyranosamino derivative having the formula GlcNRnull-Rnullnull, wherein NRnull is an azido, 2-N-acetyl-, 2-N-phtalimido, or an organic group bound to the 2-N-group of glucosamine, wherein Rnullnull is a glycosidically bound fluoro or is an O-, C-, N- or S-glycosidically bound aliphatic or aromatic compound, with the proviso that if NRnull is NRAc then Rnullnull is not OH and if NRnull is not NHAc then Rnullnull may be OH, in the presence of Bullera singularis or an E.C. group 3.2 glycosidase of essentially the same structure as an E.C. Group 3.2 glycosidase obtained from Bullera singularis to form the lactosamine derivative; and optionally isolating the compound with null1-4 linkage which contains the lactosamine structure.
摘要翻译：公开了一种制备具有β1-4键的化合物的方法，其含有所述乳糖胺结构，其包括使至少一种供体物质GalbetaOR与R为有机基团反应，以及至少一种受体物质，其为具有式GlcNR“'的吡喃葡萄糖氨基衍生物 -R“'，其中NR”是叠氮基，2-N-乙酰基，2-N-邻苯二甲酰亚胺或与2-N-基葡糖胺结合的有机基团，其中R“'是糖苷基结合的氟或是O-，C-，N-或S-糖苷结合的脂族或芳族化合物，条件是如果NR“为NRAc，那么R”'不为OH，并且如果NR“不是NHAc，则在Bullera singularis存在的情况下，R“可以是OH，或者是由从Bullera singularis获得的EC 3.2族糖苷酶基本上相同结构的EC组3.2糖苷酶形成乳糖胺衍生物; 并任选地用包含乳糖胺结构的β1-4键分离化合物。

10. 发明申请

US20040072303A1 Method of preparing a guanosine-group compound and an intermediate thereof 有权
标题翻译：制备鸟苷类化合物及其中间体的方法
公开(公告)号：US20040072303A1
公开(公告)日：2004-04-15
申请号：US10615265
申请日：2003-07-08
申请人： YUKI GOSEI KOGYO CO., LTD.
发明人： Yoichi Mikami , Seiichiro Matsumoto , Yoshinori Hayashi , Toyoki Sato
IPC分类号： C12P019/30
CPC分类号： C12P19/40
摘要： A glyoxal-guanosine-group compound is prepared either by reacting glyoxal-guanine with any one of ribose-1-phosphate and 2-deoxyribose-1-phosphate in the presence of purine nucleoside phosphorylase, or by reacting glyoxal-guanine with any one selected from the group consisting of uridine, 2null-deoxyuridine and thymidine, together with phosphate ion, in the presence of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase. The glyoxal-guanosine-group compound is then decomposed by alkali, whereby a guanosine-group compound consisting of guanosine and 2null-deoxyguanosine is prepared.
摘要翻译：通过使乙二醛 - 鸟嘌呤与嘌呤核苷磷酸化酶存在下的核糖-1-磷酸和2-脱氧核糖-1-磷酸的任一种反应，或通过使乙二醛 - 鸟嘌呤与选定的任何一种反应来制备乙二醛 - 鸟苷类化合物在嘌呤核苷磷酸化酶和嘧啶核苷磷酸化酶存在下，由尿苷，2'-脱氧尿苷和胸苷以及磷酸根离子组成的组。然后将乙二醛 - 鸟苷类化合物用碱分解，由此制备由鸟苷和2'-脱氧鸟苷组成的鸟苷类化合物。

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