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1. 发明授权

US07060455B1 Broad specificity DNA damage endonuclease 失效
标题翻译：广泛的特异性DNA损伤内切核酸酶
公开(公告)号：US07060455B1
公开(公告)日：2006-06-13
申请号：US09724296
申请日：2000-11-28
申请人： Paul W. Doetsch , Angela M. Avery , Balveen Kaur
发明人： Paul W. Doetsch , Angela M. Avery , Balveen Kaur
IPC分类号： C12N19/16 , C12O1/66 , C12P19/34
CPC分类号： C12N9/22 , A61K38/00
摘要： The present disclosure describes DNA damage endonucleases which exhibit broad specificity with respect to the types of structural aberrations in double stranded DNA. These enzymes recognize double stranded DNA with distortions in structure, wherein the distortions result from photoproducts, alkylation, intercalation, abasic sites, mismatched base pairs, insertion deletion loops, cisplatin adducts and other types of base damage (for example, uracil resulting from cytosine deamination). The UVDE (Uve1p) of Schizosaccharomyces pombe, certain truncated forms of that UVDE (lacking from about 100 to about 250 amino acids of N-terminal sequence) and certain endonucleases from Homo sapiens, Neurospora crassa, Bacillus subtilis, Bacillus anthracis, Methanococcus jannaschii, and Deinococcus radiodurans. The present disclosure further provides methods for cleaving double stranded DNA having structural distortions as set forth herein using the exemplified endonucleases or their stable, functional truncated derivatives.
摘要翻译：本公开描述了针对双链DNA中的结构畸变类型显示出广泛特异性的DNA损伤内切核酸酶。这些酶识别结构失真的双链DNA，其中由光产物，烷基化，嵌入，脱碱基位点，错配碱基对，插入缺失环，顺铂加合物和其他类型的碱损伤（例如，由胞嘧啶脱氨基产生的尿嘧啶））。粟酒裂殖酵母的UVDE（Uve1p），该UVDE的某些截短形式（缺少N-末端序列的约100至约250个氨基酸）和来自智人，粗糙脉孢菌，枯草芽孢杆菌，炭疽芽孢杆菌，季节性嗜甲酸球菌的某些内切核酸酶，和放线杆菌（Deinococcus radiodurans）。本公开进一步提供了使用示例性内切核酸酶或其稳定的功能性截短的衍生物来切割具有如本文所阐述的结构失真的双链DNA的方法。

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