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1. 发明授权

US06660500B2 Production of peptides in plants as viral coat protein fusions 失效
公开(公告)号：US06660500B2
公开(公告)日：2003-12-09
申请号：US09755836
申请日：2001-01-05
申请人： Thomas H. Turpen , Stephen J. Reinl , Laurence K. Grill
发明人： Thomas H. Turpen , Stephen J. Reinl , Laurence K. Grill
IPC分类号： C12N1562
CPC分类号： C07K14/005 , C07K14/415 , C07K14/445 , C07K2319/00 , C12N9/0059 , C12N9/0071 , C12N9/1074 , C12N9/14 , C12N9/16 , C12N9/18 , C12N9/20 , C12N9/6459 , C12N9/78 , C12N9/84 , C12N15/8203 , C12N15/8216 , C12N15/8242 , C12N15/8257 , C12N15/8289 , C12N15/86 , C12N2770/00022 , C12N2770/32722 , C12N2830/38 , C12P41/003 , C12Y114/18001 , C12Y302/01031 , C12Y304/21069 , Y02A50/412 , Y10S977/917
摘要： The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have many uses. Such uses include use as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity, including immunity against parasitic infections.

2. 发明授权

US06168932A Recombinant DTctGMCSF fusion toxin in a baculovirus expression vector system 失效
标题翻译：重组DTctGMCSF融合毒素在杆状病毒表达载体系统中
公开(公告)号：US06168932A
公开(公告)日：2001-01-02
申请号：US09114503
申请日：1998-07-13
申请人： Fatih M. Uckun , Mark D. Williams , Alexander Rostovstev
发明人： Fatih M. Uckun , Mark D. Williams , Alexander Rostovstev
IPC分类号： C12N1562
CPC分类号： C07K14/535 , C07K2319/00 , C12N2799/026
摘要： A soluble form of DTctGMCSF is produced with high yield in insect cells, preferably using the baculovirus expression vector system (BEVS).
摘要翻译：在昆虫细胞中，优选使用杆状病毒表达载体系统（BEVS），以高产率产生可溶形式的DTctGMCSF。

3. 发明授权

US06617135B1 Multiple cytokine protein complexes 有权
标题翻译：多种细胞因子蛋白复合物
公开(公告)号：US06617135B1
公开(公告)日：2003-09-09
申请号：US09634368
申请日：2000-08-09
申请人： Stephen D. Gillies , Kin-Ming Lo
发明人： Stephen D. Gillies , Kin-Ming Lo
IPC分类号： C12N1562
CPC分类号： C07K16/30 , A61K38/193 , A61K38/2013 , A61K38/208 , A61K47/646 , A61K47/6813 , A61K48/00 , A61K2039/505 , C07K14/52 , C07K14/535 , C07K14/5434 , C07K14/55 , C07K16/245 , C07K16/247 , C07K16/3084 , C07K2317/52 , C07K2317/74 , C07K2319/00 , C07K2319/30 , C07K2319/40 , C12N2799/027
摘要： The invention relates to protein complexes and fusion proteins including at least two different cytokine molecules. The protein complexes and fusion proteins may further include a targeting moiety such as a region of an immunoglobulin. Methods of using the protein complexes and fusion proteins are also disclosed.
摘要翻译：本发明涉及包括至少两种不同细胞因子分子的蛋白质复合物和融合蛋白。蛋白质复合物和融合蛋白可以进一步包括靶向部分，例如免疫球蛋白的区域。还公开了使用蛋白质复合物和融合蛋白的方法。

4. 再颁专利

USRE37343E1 Expression and secretion of heterologous proteins in yeast employing truncated alpha-factor leader sequences 有权
标题翻译：使用截短的α因子前导序列在酵母中表达和分泌异源蛋白
公开(公告)号：USRE37343E1
公开(公告)日：2001-08-28
申请号：US09140858
申请日：1998-08-27
申请人： Patricia Tekamp-Olson
发明人： Patricia Tekamp-Olson
IPC分类号： C12N1562
CPC分类号： C12N15/81 , C07K14/39 , C07K14/62 , C07K14/65 , C07K2319/02
摘要： A yeast &agr;-factor expression system is provided comprised of a truncated leader sequence, containing the &agr;-factor signal peptide and one glycosylation site, linked by a processing site to a non-yeast protein-encoding sequence.
摘要翻译：提供酵母α因子表达系统，其包含截短的前导序列，其含有α-因子信号肽和一个糖基化位点，通过加工位点连接到非酵母蛋白质编码序列。

5. 发明授权

US06274344B1 Methods for recombinant microbial production of fusion proteins and BPI-derived peptides 失效
标题翻译：重组微生物生产融合蛋白和BPI衍生肽的方法
公开(公告)号：US06274344B1
公开(公告)日：2001-08-14
申请号：US09217352
申请日：1998-12-21
申请人： Marc D. Better
发明人： Marc D. Better
IPC分类号： C12N1562
CPC分类号： C12N15/62 , A61K38/00 , C07K14/4742 , C07K2319/00 , C07K2319/02
摘要： The present invention relates to methods and materials for the recombinant microbial production of fusion proteins and peptides derived from or based on Domain I (amino acids 17-45), Domain II (amino acids 65-99) and Domain III (amino acids 142-169) of bactericidal/permeability-increasing protein (BPI).
摘要翻译：本发明涉及用于重组微生物产生融合蛋白的方法和材料，以及衍生自或基于结构域I（氨基酸17-45），结构域II（氨基酸65-99）和结构域III（氨基酸142- 169）的杀菌/渗透性增加蛋白（BPI）。

6. 发明授权

US06753167B2 Preparation of heterologous proteins on oil bodies 失效
标题翻译：在油体上制备异源蛋白质
公开(公告)号：US06753167B2
公开(公告)日：2004-06-22
申请号：US09893525
申请日：2001-06-29
申请人： Maurice M. Moloney , Gijs van Rooijen
发明人： Maurice M. Moloney , Gijs van Rooijen
IPC分类号： C12N1562
CPC分类号： C12N9/6483 , C07K14/415 , C07K14/545 , C07K14/61 , C07K14/8139 , C07K14/815 , C07K2319/00 , C07K2319/02 , C12N9/6429 , C12N15/8222 , C12N15/8234 , C12N15/8241 , C12N15/8242 , C12N15/8257 , C12Y304/21005 , C12Y304/23004
摘要： The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides may include but are not limited to: seed storage proteins, enzymes, bioactive peptides, antibodies and the like. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells. Additionally the invention provides a method of using recombinant proteins associated with seed oil bodies released during seed germination for expression of polypeptides that afford protection to seedlings from pathogens. Finally, the persistent association of oil body proteins with the oil body can be further utilized to develop a biological means to create novel immobilized enzymes useful for bioconversion of substrates.
摘要翻译：本发明涉及具有独特特征的称为油体蛋白基因的一类基因的用途。这些特征的发现允许本发明用于生产重组蛋白质的方法，其中感兴趣的蛋白质可以容易地与其他宿主细胞组分分离。本发明进一步举例说明了利用油体蛋白和油体基因在许多生物体，特别是植物种子中表达目标多肽的独特特征的方法。所述多肽可以包括但不限于：种子储存蛋白，酶，生物活性肽，抗体等。还可以修饰本发明以从非植物宿主细胞中回收与油体蛋白融合的重组多肽。另外，本发明提供了一种使用与种子发芽期间释放的种子油体相关的重组蛋白的方法，用于表达能够保护来自病原体的幼苗的多肽。最后，油体蛋白与油体的持续结合可以进一步利用，以开发生物学手段来产生可用于生物转化底物的新型固定化酶。

7. 发明授权

US06750046B2 Preparation of thioredoxin and thioredoxin reductase proteins on oil bodies 失效
标题翻译：在油体上制备硫氧还蛋白和硫氧还蛋白还原酶蛋白
公开(公告)号：US06750046B2
公开(公告)日：2004-06-15
申请号：US09897425
申请日：2001-07-03
申请人： Maurice M. Moloney , Bipin K. Dalmia
发明人： Maurice M. Moloney , Bipin K. Dalmia
IPC分类号： C12N1562
CPC分类号： C07K14/415 , C07K14/545 , C07K14/61 , C07K14/8139 , C07K14/815 , C07K2319/00 , C07K2319/02 , C12N9/6429 , C12N9/6483 , C12N15/8222 , C12N15/8234 , C12N15/8241 , C12N15/8242 , C12N15/8257 , C12Y304/21005 , C12Y304/23004
摘要： The present invention relates to the use of a class of genes called oil body protein genes that have unique features. The discovery of these features allowed the invention of methods for the production of recombinant proteins wherein a protein of interest can be easily separated from other host cell components. The invention is further exemplified by methods for exploitation of the unique characteristics of the oil body proteins and oil body genes for expression of polypeptides of interest in many organisms, particularly plant seeds. Said polypeptides include thioredoxin and/or thioredoxin reductase. The invention can also be modified to recover recombinant polypeptides fused to oil body proteins from non-plant host cells.
摘要翻译：本发明涉及具有独特特征的称为油体蛋白基因的一类基因的用途。这些特征的发现允许本发明用于生产重组蛋白质的方法，其中感兴趣的蛋白质可以容易地与其他宿主细胞组分分离。本发明进一步举例说明了利用油体蛋白和油体基因在许多生物体，特别是植物种子中表达目标多肽的独特特征的方法。所述多肽包括硫氧还蛋白和/或硫氧还蛋白还原酶。还可以修饰本发明以从非植物宿主细胞中回收与油体蛋白融合的重组多肽。

8. 发明授权

US06673573B2 Expression of immunoglobulin-cytokine fusion proteins in malignant B cells 失效
标题翻译：免疫球蛋白 - 细胞因子融合蛋白在恶性B细胞中的表达
公开(公告)号：US06673573B2
公开(公告)日：2004-01-06
申请号：US09064026
申请日：1998-04-21
申请人： Ralph Mocikat
发明人： Ralph Mocikat
IPC分类号： C12N1562
CPC分类号： C12N15/907 , C07K14/52 , C07K2317/21 , C07K2319/00 , C07K2319/30
摘要： According to the invention, there is provided a vector for the expression of immunoglobulin-cytokine fusion proteins in malignant B cells at least containing operably linked to each other (a) a region of at least 1.5 kb which is homologous to a region of the &mgr; intron or the &kgr; intron and which lacks a functional C&mgr; or C&kgr; enhancer or contains a non-functional C82 or C&kgr; enhancer; (b) at least one DNA sequence encoding a domain of an immunoglobulin or a part thereof; (c) a DNA sequence encoding a cytokine; and (d) a marker gene selectable in eukaryotic B cells and lacking a functional enhancer region wherein the expression of said marker following integration is controlled by the cellular C&mgr; or C&kgr; enhancer.
摘要翻译：根据本发明，提供了用于在恶性B细胞中表达免疫球蛋白 - 细胞因子融合蛋白的载体，其至少包含彼此可操作地连接（a）至少1.5kb的与mu区域同源的区域内含子或κ内含子，其缺少功能性Cmu或Ckappa增强子或含有非功能性C82或Ckappa增强子;（b）至少一种编码免疫球蛋白或其部分的结构域的DNA序列;（c）DNA序列编码细胞因子; 和（d）在真核B细胞中可选择的缺乏功能增强子区的标记基因，其中整合后所述标记的表达由细胞Cmu或Ckappa增强子控制。

9. 发明授权

US06426199B1 DNA 有权
标题翻译：脱氧核糖核酸
公开(公告)号：US06426199B1
公开(公告)日：2002-07-30
申请号：US09386629
申请日：1999-08-31
申请人： Andrew Darrow , Jenson Qi , Patricia Andrade-Grodon
发明人： Andrew Darrow , Jenson Qi , Patricia Andrade-Grodon
IPC分类号： C12N1562
CPC分类号： C12N9/6424 , A61K38/00
摘要： Here we describe the molecular identification of a cDNA encoding a novel serine protease we have termed protease C-E. The deduced amino acid sequence, and it alignment with other well-characterized serine proteases indicates that it is a member of the S1 serine protease family. We have found that the protease C-E mRNA is expressed in pancreas, placenta, prostate, small intestine, stomach, spleen, fibroblasts and epidermis, as well as in certain regions of the brain i.e., cerebellum, cerebral cortex, pituitary and hippocampus. Enzymatically active protease C-E, as produced using the methodologies described herein, is amenable to further biochemical analyses for the identification of physiological substrates and specific modulators.
摘要翻译：在这里，我们描述了编码我们称为蛋白酶C-E的新型丝氨酸蛋白酶的cDNA的分子鉴定。推导的氨基酸序列，并与其他良好表征的丝氨酸蛋白酶的比对表明它是S1丝氨酸蛋白酶家族的成员。我们发现蛋白酶C-E mRNA在胰腺，胎盘，前列腺，小肠，胃，脾脏，成纤维细胞和表皮以及脑的某些区域即小脑，大脑皮层，垂体和海马中表达。使用本文所述的方法产生的酶活性蛋白酶C-E适用于鉴定生理底物和特异性调节剂的进一步的生物化学分析。

10. 发明授权

US06174721B1 Chimeric proteins 失效
标题翻译：嵌合蛋白
公开(公告)号：US06174721B1
公开(公告)日：2001-01-16
申请号：US08943682
申请日：1997-10-14
申请人： Michael A. Innis , Abla A. Creasey
发明人： Michael A. Innis , Abla A. Creasey
IPC分类号： C12N1562
CPC分类号： C07K14/8114 , A61K38/00 , C07K2319/00
摘要： Chimeric proteins possessing Kunitz-type domain 1 of TFPI-2 and Kunitz-type domain 2 of TFPI are disclosed, as are muteins of TFPI and TFPI-2. Nucleic acid sequences, expression vectors and transformed host cells encoding and capable of producing the disclosed chimeric proteins and muteins are also disclosed. Finally, methods for prevention and treatment of septic shock using the chimeric proteins and muteins are disclosed.
摘要翻译：公开了具有TFPI-2的Kunitz型结构域1和TFPI的Kunitz型结构域2的嵌合蛋白质，以及TFPI和TFPI-2的突变蛋白。还公开了核酸序列，表达载体和编码并能够产生所公开的嵌合蛋白和突变蛋白的转化宿主细胞。最后，公开了使用嵌合蛋白质和突变蛋白质预防和治疗败血性休克的方法。

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