专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2021132185A1 CELL CULTURE EVALUATION SYSTEM 审中-公开
公开(公告)号：WO2021132185A1
公开(公告)日：2021-07-01
申请号：PCT/JP2020/047754
申请日：2020-12-21
申请人： NIHON KOHDEN CORPORATION , OSAKA UNIVERSITY
发明人： MAKINO Hodaka , KUBO Hirotsugu , KINOOKA Masahiro
IPC分类号： C12M1/34 , C12M1/36 , G06T7/00 , C12M41/36 , C12M41/46 , C12M41/48
摘要： The presently disclosed subject matter provides a cell culture evaluation system including a measurement unit, a first storage unit, a second storage unit, an evaluation unit and an output unit, wherein: the measurement unit includes a sensor that acquires evaluation target data about motion or a state of a culture tool; the first storage unit stores the evaluation target data; the second storage unit stores reference data; the evaluation unit compares the evaluation target data with the reference data so as to evaluate an execution state of a cell culture process; and the output unit outputs an evaluation result created by the evaluation unit.

2. 发明申请

WO2021254958A1 METHOD AND SYSTEM OF PRODUCING A LIBRARY OF MICROORGANISMS 审中-公开
公开(公告)号：WO2021254958A1
公开(公告)日：2021-12-23
申请号：PCT/EP2021/065961
申请日：2021-06-14
申请人： BIOMILLENIA SAS
发明人： DU, Guansheng , THOMAS, Laurine , DAJKOVIC, Aleksander , LOEFFERT, Dirk
IPC分类号： B01L3/00 , C12M1/34 , C40B50/08 , C40B60/14 , C12Q1/02 , C12M1/12 , C12M1/00 , G01N15/14 , C12M3/06 , C40B40/02 , C40B60/08 , G01N15/00 , B01L2200/0652 , B01L2200/12 , B01L3/502784 , C12M23/16 , C12M25/01 , C12M41/46 , G01N15/1459 , G01N2015/003 , G01N2015/149
摘要： In a first aspect, the present invention relates to a method of producing a library of microorganisms, the method comprising the steps of: a. providing a first fluid comprising at least one single cell, b. dispersing said first fluid comprising at least one single cell in a second fluid, thereby obtaining a plurality of single-layer microfluidic droplets, wherein at least one single- layer microfluidic droplet comprises at least one single cell, wherein the second fluid is immiscible with the first fluid, c. optionally, adding to said at least one single-layer microfluidic droplet a third fluid comprising a sensing compound, wherein the third fluid is miscible with said first fluid, and wherein the third fluid is immiscible with said second fluid, d. injecting said at least one single-layer microfluidic droplet optionally comprising the sensing compound into a fourth fluid, wherein said fourth fluid is immiscible with said second fluid, thereby obtaining at least one double-layer microfluidic droplet, e. dispensing said at least one double-layer microfluidic droplet into a culture medium based on the viability of the cell, f. incubating said culture medium, thereby obtaining said library. In a second aspect, the present invention relates to a system comprising: a. a first microfluidic chip for producing a plurality of single-layer microfluidic droplets wherein at least one single-layer microfluidic droplet comprisesat least one single cell, b. a first microfluidic device for collecting said plurality of single-layer microfluidic droplets, c. a second device for adding a sensing compound into said at least one single-layer microfluidic droplet comprising at least one single cell, d. a second microfluidic chip for producing a double-layer microfluidic droplet, and e. a dispensing unit. In a third aspect, the present invention relates to the use of the method according to the first aspect of the present invention in a system according to the second aspect of the present invention.

3. 发明申请

WO2023028224A2 IN VITRO MYOCARDIAL TISSUE SCREENING DEVICES, SYSTEMS, AND METHODS 审中-公开
公开(公告)号：WO2023028224A2
公开(公告)日：2023-03-02
申请号：PCT/US2022/041516
申请日：2022-08-25
申请人： CLEMSON UNIVERSITY RESEARCH FOUNDATION
发明人： RICHARDSON, William , COEYMAN, Samuel , HEYWOOD, Jonathan , POTTER, Michael J.
IPC分类号： C12M3/00 , C12M1/42 , C12N5/077 , C12M21/08 , C12M23/12 , C12M35/04 , C12M41/46 , G01N33/5082
摘要： Various cardio tissue testing wells with an actuable attachment structure therein, and testing systems incorporating such wells therein. The various well embodiments can include an actuable attachment structure that is actuated by external energy, such as a magnetic field, fluidic pressure, or electrical actuation. The various system embodiments can include a controller, a power source, and a testing plate containing a plurality of wells, wherein each well includes an actuable attachment structure.

4. 发明申请

WO2022097150A2 AN INSERT CHIP AND A SYSTEM COMPRISING SAME FOR CELL CULTURE 审中-公开
公开(公告)号：WO2022097150A2
公开(公告)日：2022-05-12
申请号：PCT/IL2021/051317
申请日：2021-11-08
申请人： RAMOT AT TEL AVIV UNIVERSITY LTD.
发明人： MAOZ, Ben Meir , RAUTI, Rossana , ESS, Adi , KIRI, Mark Dan
IPC分类号： C12M1/12 , C12M3/00 , C12M1/34 , C12M3/06 , C12M1/42 , C12M21/08 , C12M23/00 , C12M23/16 , C12M25/02 , C12M25/04 , C12M35/08 , C12M41/46
摘要： Provided herein is an insert chip and a cell culture system including the same, adapted for culturing a plurality of cell populations under various flow patterns.

5. 发明申请

WO2022239960A1 인간 줄기세포 유래 심근세포를 이용한 약물의 심장 안전성 평가 방법 审中-公开
公开(公告)号：WO2022239960A1
公开(公告)日：2022-11-17
申请号：PCT/KR2022/004331
申请日：2022-03-28
申请人： (주)넥셀
发明人： 우동훈 , 안지은 , 김수빈 , 윤슬기 , 연승희 , 장진혁
IPC分类号： G01N33/50 , C12M1/34 , G01N27/327 , C12M41/46 , G01N33/5061
摘要： 본 발명은 인간 전분화능 줄기세포 유래 심근세포를 이용한 약물의 심장 안전성 평가 방법에 관한 것으로, 인간 줄기세포를 배양하여 심근세포로 분화시켜 준비하는 A단계; 피브로넥틴(Fibronectin)을 DPBS(Dulbecco's phosphate buffered saline)를 이용해 50μg/ml의 농도로 희석시키는 B단계; 상기 B단계를 통해 50μg/ml의 농도로 희석된 피브로넥틴 희석액을 MEA(Multielectrode arrays) 플레이트(Plate)에 다수개로 배열된 각 웰(Well) 중앙에 구비된 전극(Electrode)의 모든 도트(Dots)들을 덮도록 첨가하고, 상기 MEA 플레이트의 웰 사이 공간에 DPBS를 첨가하여 채우는 C단계; 상기 C단계를 통해 웰과 웰 사이 공간에 각각 피브로넥틴 희석액과 DPBS가 첨가된 상기 MEA 플레이트를 37℃의 온도 환경 및 5%의 CO2 환경을 갖춘 세포 배양기에 넣어 50분 내지 60분간 코팅 진행하는 D단계; A단계를 통해 준비된 인간 줄기세포 유래 심근세포를 상기 D단계를 통해 코팅 진행이 완료된 상기 MEA 플레이트의 특정 웰에서 피브로넥틴 희석액을 제거 후 전극의 모든 도트(Dots)들을 덮도록 파종(Seeding)하는 E단계; 상기 E단계를 통해 인간 줄기세포 유래 심근세포가 파종된 상기 MEA 플레이트의 웰의 특정 웰에 소정의 배지(Medium)를 추가적으로 첨가한 뒤 상기 MEA 플레이트를 세포배양기 내에서 배양하는 F단계; 및 상기 F단계를 통해 소정의 기간 동안 배양을 거친 상기 MEA 플레이트의 인간 줄기세포 유래 심근세포가 파종된 웰들 각각에 평가 대상 약물의 첨가유무 및 첨가농도에 따른 Beat Rate, Spike Amplitude 및 Field Potential Duration에 관한 정보값의 변화를 상기 MEA 플레이트의 각각의 웰 내에 구비된 전극(Electrode)과 전기적으로 연결된 MEA(Multielectrode arrays) 측정기기를 이용해 측정하고, 측정된 정보값들을 기반으로 상기 평가 대상 약물의 심장 안전성을 평가하는 G단계;를 포함한다.

6. 发明申请

WO2021261861A1 약제내성 결핵균 검출용 배지 조성물 및 이를 이용한 약제내성 결핵균 검출 키트 审中-公开
公开(公告)号：WO2021261861A1
公开(公告)日：2021-12-30
申请号：PCT/KR2021/007753
申请日：2021-06-21
申请人： (주)어게인비에스
发明人： 박보신 , 최결 , 박은용
IPC分类号： C12Q1/04 , C12M1/34 , C12M41/46
摘要： 본 발명에서는 약제내성 결핵균 검출용 배지 조성물 및 이를 이용한 약제내성 결핵균 검출 키트가 개시된다. 본 발명에 따른 배지 조성물 및 키트는 결핵균의 증식(성장)의 저해가 없고 증식된 약제내성 결핵균의 집락의 경계가 분명하고 결핵균의 집락 색상이 배지 조성물과 보색대비 되어서, 육안으로 쉽게, 간편하게 그리고 정확하게 결핵균의 집락을 계수하여 검출할 수 있다.

7. 发明申请

WO2021121447A1 SYSTEM AND METHOD FOR AXONAL INJURY ASSAYS 审中-公开
公开(公告)号：WO2021121447A1
公开(公告)日：2021-06-24
申请号：PCT/CZ2020/050098
申请日：2020-12-16
申请人： FAKULTNI NEMOCNICE U SV. ANNY V BRNE
发明人： STOKIN, Gorazd B. , POZO DEVOTO, Victorio , LACOVICH, Valentina
IPC分类号： B01L3/00 , C12M3/06 , C12M1/00 , C12M1/34 , G01N33/50 , B01L2300/0816 , B01L2300/0861 , B01L2400/0487 , B01L3/5027 , C12M23/16 , C12M23/34 , C12M35/04 , C12M41/36 , C12M41/46 , G01N33/5058
摘要： The present invention provides an axonal injury assay device comprising - a syringe pump fitted with a syringe; - a microfluidic chamber, said microfluidic chamber containing a somatic compartment and an axonal compartment, wherein the somatic compartment and the axonal compartment are mutually connected by axonal channels, wherein the axonal channels are transected by a flow channel arranged perpendicularly to the axonal channels, and wherein the flow has on one end an opening adapted to receive an output from the syringe pump and at least one reservoir on the other end. This device can be used in an axonal injury assay method comprising the steps of a) causing by means of the syringe pump a flow of medium in withdrawal direction, said flow of medium having a flow speed of 10-66 mm/sec, preferably 17-33 mm/sec; b) causing by means of the syringe pump a flow of medium at a flow speed of at least 166 mm/sec, and preferably between 166 and 1666 mm/sec or between 333 and 1000 mm/sec; c) causing by means of the syringe pump a flow of medium in inverse direction to the direction used in step b), at a flow rate differing at most by 20 %, preferably at most by 10 % from the flow rate used in step b), most preferably at substantially the same flow rate as used in step b); d) optionally causing by means of the syringe pump a flow of medium in withdrawal direction, said flow of medium having a flow speed of 10-66 mm/sec, preferably 17-33 mm/sec. The device and method allow observing the axons during the whole injury process and post injury.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式