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    • 3. 发明授权
    • Malt beverage having stabilized flavor and methods of production thereof
    • 味道稳定的麦芽饮料及其生产方法
    • US06468567B1
    • 2002-10-22
    • US09665313
    • 2000-09-19
    • Rafael Rangel-AldaoAdriana BravoBeatriz SanchezIvan Galindo-Castro
    • Rafael Rangel-AldaoAdriana BravoBeatriz SanchezIvan Galindo-Castro
    • A23C912
    • C12N9/0008C12C5/02C12C11/003C12G3/065C12H1/003
    • The present invention is directed to a method for stabilizing the flavor of a fermented malt beverage, most particularly a beer, by the addition of one or more inhibitors, blockers, reducing agents or binding agents that inactivate one or more Maillard reaction intermediates that induce staling of the flavor of fermented malt beverages. In preferred such methods, the agents used are reductase enzymes, especially aldehyde reductases, carbonyl reductases, aldose reductases, oxoaldehyde reductases and most particularly oxidoreductases such as isozymes of Old Yellow Enzyme (OYE;EC 1.6.99.1) (e.g., OYE1 and OYE2 and OYE3). The invention is also directed to the fermented malt beverage prepared by such a method, and to the use during the brewing process of reductase enzymes from naturally occurring sources, including those produced by yeasts, to stabilize the flavor of the resulting beer product and to produce a beer having a stable flavor. The invention also relates to cells which have been specifically modified, selected, or genetically engineered to express or secrete a reductase enzyme which may be used during the brewing process to stabilize the flavor of the resulting beer product and to produce a beer having a stable flavor. The invention also provides fermented malt beverages having enhanced flavor stability produced by these methods.
    • 本发明涉及通过添加一种或多种抑制剂,阻断剂,还原剂或结合剂来灭活发酵麦芽饮料,特别是啤酒的风味的方法,所述抑制剂,阻断剂,还原剂或结合剂使一种或多种诱导陈化的美拉德反应中间体失活 的发酵麦芽饮料的味道。 所用的试剂是还原酶,尤其是醛还原酶,羰基还原酶,醛糖还原酶,氧代醛还原酶,最特别的氧化还原酶如老黄酶(OYE; EC 1.6.99.1)的同功酶(例如,OYE1和OYE2和 OYE3)。 本发明还涉及通过这种方法制备的发酵麦芽饮料,以及在酿造过程中从天然存在的来源(包括由酵母生产的那些)中还原酶的用途,以稳定所得啤酒产品的风味并产生 一种具有稳定味道的啤酒。 本发明还涉及经特异性修饰,选择或遗传工程化以表达或分泌可在酿造过程中使用的还原酶的细胞,以稳定所得啤酒产品的风味并生产具有稳定味道的啤酒 。 本发明还提供通过这些方法产生的具有增强的风味稳定性的发酵麦芽饮料。
    • 5. 发明授权
    • Natural biogenerated cheese flavoring system
    • 天然生物发酵奶酪调味系统
    • US06406724B1
    • 2002-06-18
    • US09659459
    • 2000-09-12
    • Divya Shree ReddyJames William MoranBashir Aideloje ElaborDavid Webb MehnertRudolf Johann Fritsch
    • Divya Shree ReddyJames William MoranBashir Aideloje ElaborDavid Webb MehnertRudolf Johann Fritsch
    • A23C912
    • A23L27/25A23L27/206
    • A natural biogenerated cheese flavoring system is provided which can be used to prepare very different cheeses having desired flavor profiles. More specifically, the present cheese flavoring system contains a sulfury-cheddar flavored component, a creamy-buttery flavored component, and a cheesy flavored component. Each of these flavored components can be used as flavor building blocks with their own specific flavor profiles and/or characteristics. Using various combinations of these flavored components, cheeses having a wide variety of flavors can be produced. The flavored components are separately prepared from a highly concentrated milk substrate using compositions (e.g., specific enzymes, cultures, and additives) and process conditions designed to provide the flavored components having specific flavor profiles and/or characteristics. The flavor concentrates can be used in process cheese, process cheese-type products, or other cheeses to produce very different cheeses with desired flavor profiles. The flavor concentrates can also be used as a natural flavoring system in other food products.
    • 提供天然的生物发酵乳酪调味系统,其可用于制备具有所需风味特征的非常不同的奶酪。 更具体地,本发明的奶酪调味系统含有硫磺切屑调味成分,奶油质调味成分和芝士风味成分。 这些调味组分中的每一种都可以用作具有其自身特定风味特征和/或特征的风味构成块。 使用这些调味组分的各种组合,可以生产具有各种风味的奶酪。 使用组合物(例如特定酶,培养物和添加剂)和设计用于提供具有特定风味特征和/或特征的调味组分的工艺条件,由高度浓缩的牛奶底物单独制备调味组分。 风味浓缩物可用于加工奶酪,加工奶酪类产品或其他奶酪以产生具有所需风味特征的非常不同的奶酪。 风味浓缩物也可以用作其他食品中的天然调味剂系统。
    • 6. 发明授权
    • Methods for treating foodstuffs using cell free extracts from ciliates
    • 使用无纤细提取物处理食品的方法
    • US06391351B2
    • 2002-05-21
    • US09835804
    • 2001-04-16
    • German A. Valcarce
    • German A. Valcarce
    • A23C912
    • A23C9/1203A23C9/1213A23C9/1216A23L5/25A23L5/28A23L15/00A23L15/25A23V2002/00A23V2200/3262
    • The present invention provides methods for: (a) concentrating cholesterol-desaturase activity in a cell free extract, wherein the cell free extract can be a homogenate fraction, a microsomal fraction or a desaturase-enriched fraction from a Tetrahymenidae family microorganism and (b) decreasing the cholesterol content of a foodstuff of animal origin, such as a dairy product. The methods comprise the step of treating a foodstuff by incubating the same with a cell free extract in a effective amount to enable changes in the foodstuff: (a)reduce the level of cholesterol; and (b) increase the level of at least one desaturated cholesterol derivative: &Dgr;7-dehydrocholesterol, &Dgr;22 dehydrocholesterol and/or &Dgr;7,22 bisdehydrocholesterol.
    • 本发明提供了以下方法:(a)在无细胞提取物中浓缩胆固醇 - 去饱和酶活性,其中所述无细胞提取物可以是来自四膜科科微生物的匀浆级分,微粒体级分或富含去饱和酶的级分,和(b) 降低动物来源的食品如乳制品的胆固醇含量。 所述方法包括用有效量的无细胞提取物将其食物处理以使食物变化的步骤:(a)降低胆固醇水平; 和(b)增加至少一种去饱和胆固醇衍生物:DELTA7-脱氢胆固醇,DELTA22脱氢胆固醇和/或DELTA7,22双氢胆固醇的水平。
    • 7. 发明授权
    • Wheyless process for production of natural mozzarella cheese
    • 生产天然无乳酪干酪的无糖过程
    • US06372268B1
    • 2002-04-16
    • US09863183
    • 2001-05-23
    • Richard Stuart SilverXiao-Qing HanRichard LincourtMaria Lucrecia Cardona
    • Richard Stuart SilverXiao-Qing HanRichard LincourtMaria Lucrecia Cardona
    • A23C912
    • A23C19/028A23C19/0684
    • The present invention provides a wheyless process for preparing natural mozzarella cheese using dry dairy ingredients. This process enables the manufacture of cheese from non-perishable or shelf-stable ingredients such as dried milk protein concentrate and anhydrous milkfat. This enables greater flexibility in the location of cheese manufacturing facilities as handling and/or transporting large quantities of fresh milk is not required. Also, in utilizing such a process, the need for refrigerated storage of the fresh milk would be minimal. The dry dairy ingredients used in the present invention comprise milk protein concentrates and blends of milk protein concentrates with up to about 50 percent of a second dry dairy ingredient selected from the group consisting of whey protein concentrate, whey protein isolate, calcium caseinate, sodium caseinate, rennet casein, acid casein, nonfat dry milk, and mixtures thereof.
    • 本发明提供了一种使用干乳制品成分制备天然麦芽干酪乳酪的无干燥方法。 该方法能够从不易腐或稳定的成分制造干酪,例如干乳蛋白浓缩物和无水乳脂。 这使得乳酪制造设施的位置具有更大的灵活性,因为不需要处理和/或运输大量的新鲜牛奶。 此外,在使用这种方法时,对新鲜牛奶的冷藏储存的需要将是最小的。 用于本发明的干乳制品成分包括乳蛋白浓缩物和乳蛋白浓缩物与高达约50%的第二干乳制品成分的混合物,其中乳制品成分选自乳清蛋白浓缩物,乳清蛋白分离物,酪蛋白酸钙,酪蛋白酸钠 ,瑞汀酪蛋白,酸性酪蛋白,脱脂奶粉及其混合物。