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1. 发明公开

CN108613925A 一种沉积镍铬镁包覆层硅藻土的化学分析方法无效
公开(公告)号：CN108613925A
公开(公告)日：2018-10-02
申请号：CN201611137145.1
申请日：2016-12-12
申请人：沈阳云美科技有限公司
发明人：曹玉坤
IPC分类号： G01N21/01 , G01N21/75
CPC分类号： G01N21/01 , G01N21/75 , G01N2021/751
摘要：本发明涉及一种化学分析方法，具体涉及一种沉积镍铬镁包覆层硅藻土的化学分析方法。本发明的技术方案如下：一种沉积镍铬镁包覆层硅藻土的化学分析方法，一：分析仪器及化学试剂准备；二：样品处理；三：配制标准溶液；四：仪器测量。本发明的沉积镍铬镁包覆层硅藻土的化学分析方法，能够缩短分析周期，提高分析效率、准确度和精密度。

2. 发明公开

CN107091832A UV/H2O2降解水中典型有机物反应速率常数的测定方法有权
公开(公告)号：CN107091832A
公开(公告)日：2017-08-25
申请号：CN201710321043.3
申请日：2017-05-09
申请人：山东省城市供排水水质监测中心
发明人：贾瑞宝 , 宋武昌 , 孙韶华 , 冯桂学
IPC分类号： G01N21/75 , G01N21/33 , G01N31/00 , C02F1/32 , C02F1/72 , C02F101/30
CPC分类号： G01N21/75 , C02F1/32 , C02F1/722 , C02F2101/30 , C02F2101/305 , C02F2305/023 , G01N21/33 , G01N31/005 , G01N2021/751
摘要：本发明公开了一种实际水中UV/H2O2降解典型有机物反应速率常数的测定方法，包括：(1)采用配套UV/H2O2高级氧化装置开展静态实验；(2)配套UV/H2O2高级氧化装置，为静态实验装置；(3)实际水中UV/H2O2降解UV254的一级反应动力学常数测定；(4)实际水中UV254降解的二级反应动力学常数测定；(5)竞争动力学模型；(6)实际水中UV254和典型有机物C的一级反应动力学常数测定rUV和r；(7)典型有机物的二级反应动力学常数测定。本发明量化了实际水中NOM、无机离子等对典型痕量有机物的降解速率影响，为UV/H2O2高级氧化工艺实际应用提供了理论支持和工艺参数设计依据。

3. 发明公开

CN106290326A 检测脂多糖的比色传感器、其制备方法和应用失效
公开(公告)号：CN106290326A
公开(公告)日：2017-01-04
申请号：CN201610577032.7
申请日：2016-07-21
申请人：上海大学
发明人：李根喜 , 张娟 , 李得凤
IPC分类号： G01N21/78 , B82Y30/00
CPC分类号： G01N21/78 , B82Y30/00 , G01N2021/751 , G01N2021/7756 , G01N2021/7769
摘要：本发明公开了一种检测脂多糖的比色传感器、其制备方法和应用，传感器通过紫外-可见光谱技术进行检测，其特征在于利用了氨基苯硼酸修饰的磁纳米颗粒对脂多糖的识别及其类似于过氧化物酶的催化氧化活性和脂多糖类脂质双层对离子运输的阻碍作用。本发明利用了3-氨基苯硼酸与脂多糖的高特异性结合及脂多糖类脂质双层对离子运输的阻碍作用，保证了检测的高度特异性，借助磁纳米颗粒类似于过氧化物酶的催化氧化活性，提高了检测的灵敏性。通过紫外-可见光谱技术捕捉磁纳米催化H2O2氧化ABTS产生的颜色变化，实现了脂多糖的分析检测。本发明方法简单、快速、无需信号标记；灵敏度高，可以在0.00001 μg/mL到180 μg/mL范围内线性检测脂多糖；特异性强，可以有效地区分脂多糖与其他对照物质。

4. 发明授权

US5677134A Method of assay of enzymatic activity and apparatus therefor 失效
标题翻译：酶活性测定方法及其设备
公开(公告)号：US5677134A
公开(公告)日：1997-10-14
申请号：US415570
申请日：1995-04-03
申请人： Hidechika Hayashi , Yoshihiko Umegae , Yukio Mitsuhisa
发明人： Hidechika Hayashi , Yoshihiko Umegae , Yukio Mitsuhisa
IPC分类号： C12M1/34 , C12M1/40 , C12Q1/00 , C12Q1/42 , G01N21/64 , G01N21/75 , G01N33/535 , G01N33/543 , G01N33/58 , G01N33/573
CPC分类号： G01N21/64 , C12Q1/00 , G01N21/6428 , G01N33/581 , G01N2021/6421 , G01N2021/751 , G01N21/274 , Y10S435/808 , Y10S435/967 , Y10S435/968 , Y10S436/805
摘要： Method of assay of enzymatic activity, comprising projecting excitation light to a sample containing an enzyme, a substrate which forms a product by action of the enzyme, and a reference substance which is insensitive to the action of the enzyme but emits fluorescence; obtaining a first measured value of fluorescence intensity of the sample at a first wavelength region which includes fluorescence emitted by the substrate or the product at least, obtaining a second measured value of fluorescence intensity at a second wavelength region which is different from the first wavelength region for the first measured value and includes fluorescence emitted by the reference substance; and assaying the enzymatic activity from the ratio of the first measured value to the second measured value and apparatus for performing the method. The method assures high accuracy and high sensitivity of measurement in enzyme labeled immunoassay and enzyme labeled DNA hybridization.
摘要翻译：测定酶活性的方法，包括将激发光投射到含有酶的样品，通过酶的作用形成产物的底物和对酶的作用不敏感但发射荧光的参考物质; 在包括由所述基板或所述产物发射的荧光的第一波长区域获得所述样品的荧光强度的第一测量值至少获得与所述第一波长区域不同的第二波长区域处的荧光强度的第二测量值对于第一测量值并包括由参考物质发射的荧光; 以及从第一测量值与第二测量值的比值和用于执行该方法的装置测定酶活性。该方法在酶标记免疫测定和酶标记DNA杂交中保证了高精度和高灵敏度的测量。

5. 发明授权

US5460943A Method of assay of enzymatic activity 失效
标题翻译：酶活性测定方法
公开(公告)号：US5460943A
公开(公告)日：1995-10-24
申请号：US900286
申请日：1992-06-18
申请人： Hidechika Hayashi , Yoshihiko Umegae , Yukio Mitsuhisa
发明人： Hidechika Hayashi , Yoshihiko Umegae , Yukio Mitsuhisa
IPC分类号： C12M1/34 , C12M1/40 , C12Q1/00 , C12Q1/42 , G01N21/64 , G01N21/75 , G01N33/535 , G01N33/543 , G01N33/58 , G01N33/573
CPC分类号： G01N21/64 , C12Q1/00 , G01N21/6428 , G01N33/581 , G01N2021/6421 , G01N2021/751 , G01N21/274 , Y10S435/808 , Y10S435/967 , Y10S435/968 , Y10S436/805
摘要： Method of assay of enzymatic activity, including projecting excitation light to a sample containing an enzyme, a substrate which forms a product by action of the enzyme, and a reference substance which is insensitive to the action of the enzyme but emits fluorescence; obtaining a first measured value of fluorescence intensity of the sample at a first wavelength region which includes fluorescence emitted by the substrate or the product at least, obtaining a second measured value of fluorescence intensity at a second wavelength region which is different from the first wavelength region for the first measured value and includes fluorescence emitted by the reference substance; and assaying the enzymatic activity from the ratio of the first measured value to the second measured value and apparatus for performing the method. The method assures high accuracy and high sensitivity of measurement in enzyme labeled immunoassay and enzyme labeled DNA hybridization.
摘要翻译：测定酶活性的方法，包括将激发光投射到含有酶的样品，通过酶的作用形成产物的底物和对酶的作用不敏感但发射荧光的参考物质; 在包括由所述基板或所述产物发射的荧光的第一波长区域获得所述样品的荧光强度的第一测量值至少获得与所述第一波长区域不同的第二波长区域处的荧光强度的第二测量值对于第一测量值并包括由参考物质发射的荧光; 以及从第一测量值与第二测量值的比值和用于执行该方法的装置测定酶活性。该方法在酶标记免疫测定和酶标记DNA杂交中保证了高精度和高灵敏度的测量。

6. 发明授权

EP0521636B1 Method of assay of enzymatic activity and apparatus therefor 失效
标题翻译：酶活性的研究设备的方法，及为此
公开(公告)号：EP0521636B1
公开(公告)日：1997-09-17
申请号：EP92305595.8
申请日：1992-06-18
申请人： TOSOH CORPORATION
发明人： Hayashi, Hidechika , Umegae, Yoshihiko , Mitsuhisa, Yukio
IPC分类号： G01N21/64 , G01N33/53 , C12Q1/00
CPC分类号： G01N21/64 , C12Q1/00 , G01N21/274 , G01N21/6428 , G01N33/581 , G01N2021/6421 , G01N2021/751 , Y10S435/808 , Y10S435/967 , Y10S435/968 , Y10S436/805

7. 发明公开

EP3001183A1 Detection of analytes using nanoparticles as light scattering enhancers 审中-公开
标题翻译： Nachweis von分析软件Verwendung von Nanopartikeln alsLichtstreuungsverstärker
公开(公告)号：EP3001183A1
公开(公告)日：2016-03-30
申请号：EP14306480.6
申请日：2014-09-25
申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE -CNRS- , Université d'Aix Marseille
发明人： Wenger, Jérôme Charles , Rigneault, Hervé
IPC分类号： G01N21/65
CPC分类号： G01N21/272 , G01N21/51 , G01N33/54346 , G01N2021/4769 , G01N2021/751
摘要： A method for detecting the presence of an analyte (1) in a solution (2) comprising: providing at least a first and a second probes (A, B) different from each other, each probe (A,B) comprising a nanoparticle conjugated with a receptor specific to the analyte (1); contacting the solution (2) suspected of including the analyte (1) with the first and the second probes (A, B) to form a sample solution (3), wherein the sample solution (3) comprises aggregates (4) comprising the analyte (1) combined with the first and the second probes (A, B); illuminating the sample solution (3) with a light source having at least a first and a second exciting wavelengths (λ eA , λ eB ) different from each other wherein the first and the second wavelength are chosen to get specific optical responses from the first probe (A) and the second probe (B) respectively when illuminated; detecting as a function of time the light scattered by the first probe (A) at a first detection wavelength (λ dA ) and the light scattered by the second probe (B) at a second detection wavelength (λ dB ) to get a first signal and a second signal respectively; and detecting temporal coincidences between said first signal and second signal.
摘要翻译：一种用于检测溶液（2）中分析物（1）的存在的方法，包括：提供至少彼此不同的第一和第二探针（A，B），每个探针（A，B）包含纳米颗粒共轭具有对分析物（1）特异性的受体; 将疑似包含分析物（1）的溶液（2）与第一和第二探针（A，B）接触以形成样品溶液（3），其中样品溶液（3）包含包含分析物的聚集体（4）（1）与第一和第二探针（A，B）组合; 用具有至少彼此不同的第一和第二激发波长（»eA，»eB）的光源照射样品溶液（3），其中选择第一和第二波长以获得来自第一探针的特定光学响应（A）和第二探针（B）分别照射; 以第一检测波长（»dA）由第一探测器（A）散射的光和由第二探测器（B）散射的光以第二检测波长（»dB）检测作为时间的函数，以获得第一信号和第二信号; 以及检测所述第一信号和第二信号之间的时间重合。

8. 发明公开

EP0521636A1 Method of assay of enzymatic activity and apparatus therefor 失效
标题翻译： Verfahren zur Untersuchung enzymematischerAktivitätund Vorrichtunghierfür。
公开(公告)号：EP0521636A1
公开(公告)日：1993-01-07
申请号：EP92305595.8
申请日：1992-06-18
申请人： TOSOH CORPORATION
发明人： Hayashi, Hidechika , Umegae, Yoshihiko , Mitsuhisa, Yukio
IPC分类号： G01N21/64 , G01N33/53 , C12Q1/00
CPC分类号： G01N21/64 , C12Q1/00 , G01N21/274 , G01N21/6428 , G01N33/581 , G01N2021/6421 , G01N2021/751 , Y10S435/808 , Y10S435/967 , Y10S435/968 , Y10S436/805
摘要： Method of assay of enzymatic activity, comprising projecting excitation light to a sample containing an enzyme, a substrate which forms a product by action of the enzyme, and a reference substance which is insensitive to the action of the enzyme but emits fluorescence; obtaining a first measured value of fluorescence intensity of the sample at a first wavelength region which includes fluorescence emitted by the substrate or the product at least, obtaining a second measured value of fluorescence intensity at a second wavelength region which is different from the first wavelength region for the first measured value and includes fluorescence emitted by the reference substance; and assaying the enzymatic activity from the ratio of the first measured value to the second measured value and apparatus for performing the method. The method assures high accuracy and high sensitivity of measurement in enzyme labeled immunoassay and enzyme labeled DNA hybridization.
摘要翻译：测定酶活性的方法，包括将激发光投射到含有酶的样品，通过酶的作用形成产物的底物和对酶的作用不敏感但发射荧光的参考物质; 在包括由所述基板或所述产物发出的荧光的第一波长区域获得所述样品的荧光强度的第一测量值至少获得与所述第一波长区域不同的第二波长区域处的荧光强度的第二测量值对于第一测量值并包括由参考物质发射的荧光; 以及从第一测量值与第二测量值的比值和用于执行该方法的装置测定酶活性。该方法在酶标记免疫测定和酶标记DNA杂交中保证了高精度和高灵敏度的测量。

9. 发明申请

WO2017034073A1 TEST INSTRUMENT AND METHOD OF CONTROLLING THE SAME 审中-公开
标题翻译：测试仪器及其控制方法
公开(公告)号：WO2017034073A1
公开(公告)日：2017-03-02
申请号：PCT/KR2015/011140
申请日：2015-10-21
申请人： SAMSUNG ELECTRONICS CO., LTD.
发明人： SON, Yu Ri , JANG, Eun Jeong , JO, Jeong Min
IPC分类号： G01N21/82 , G01N21/31 , G01N21/59 , G01N21/55
CPC分类号： G01N21/75 , G01N35/00603 , G01N35/00712 , G01N2021/751 , G01N2021/752
摘要： Disclosed herein are a test instrument and a method of controlling the same, capable of performing a basic test on a sample of a patient, determining whether to perform an additional test, and displaying interfaces associated with a progress level, a necessary time, etc. of the additional test on a display unit when the additional test is performed, thereby enabling a user to visibly recognize information associated with a performing process of each test. The test instrument includes: a detection unit configured to apply light to at least one chamber in which a reaction between a reagent and a sample occurs and to detect an optical signal from the chamber to test the sample held in a reaction device, a control unit configured to determine whether to perform a secondary test on the sample after a primary test is performed on the sample and to control to display a secondary test progress interface showing a progress level of the secondary test when the secondary test is performed, and a display unit configured to display a primary test progress interface showing a progress level of the primary test on the sample and to display the secondary test progress interface showing the progress level of the secondary test when the secondary test is performed on the sample.
摘要翻译：本文公开了一种测试仪器及其控制方法，能够对患者的样本进行基本测试，确定是否进行附加测试，以及显示与进度水平相关的界面，必要时间等。在执行附加测试时在显示单元上的附加测试，从而使得用户能够可视地识别与每个测试的执行过程相关联的信息。测试仪器包括：检测单元，被配置为向至少一个室发射光，其中发生试剂和样品之间的反应，并且检测来自腔室的光信号，以测试保持在反应装置中的样品;控制单元被配置为在对所述样本进行初次测试之后，确定是否对所述样本进行二次测试，并且控制在进行所述次级测试时显示示出所述次级测试的进度水平的次级测试进度界面;以及显示单元配置为显示一个主测试进度界面，显示了样本的主要测试进度，并显示了在对样本进行二次测试时显示次级测试进度的次级测试进度界面。

10. 发明申请

WO2002061116A1 IN VIVO SCREENING ARRAY 审中-公开
标题翻译：在VIVO筛选阵列
公开(公告)号：WO2002061116A1
公开(公告)日：2002-08-08
申请号：PCT/US2002/001387
申请日：2002-01-16
申请人： THE GENERAL HOSPITAL CORPORATION , DOTTO, G. Paolo
发明人： DOTTO, G. Paolo
IPC分类号： C12Q1/00
CPC分类号： G01N33/5082 , A01K2217/05 , C12N2503/00 , G01N21/64 , G01N21/75 , G01N2021/751
摘要： The invention features methods of evaluating the effect of a plurality of different treatments or compounds on a tissue. The method includes providing a tissue from an animal; contacting the tissue with a plurality of treatment, forming on the tissue an array of a plurality of regions or addresses. The method includes the use of a two dimensional array member to contact the plurality of treatments with the tissue and/or to evaluate their effect. The methods are useful for screening large numbers of therapeutic or cosmetic compounds.
摘要翻译：本发明的特征在于评估多种不同处理或化合物对组织的影响的方法。该方法包括从动物提供组织; 使组织接触多个处理，在组织上形成多个区域或地址的阵列。该方法包括使用二维阵列构件与组织接触多个处理和/或评估其效果。该方法可用于筛选大量治疗或化妆品化合物。

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