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1. 发明申请

US20180282706A1 NEW LACTALDEHYDE REDUCTASES FOR THE PRODUCTION OF 1,2-PROPANEDIOL 审中-公开
公开(公告)号：US20180282706A1
公开(公告)日：2018-10-04
申请号：US15758885
申请日：2015-09-10
申请人： METABOLIC EXPLORER
发明人： Gwénaëlle CORRE , Pascale ALIPRANDI
IPC分类号： C12N9/04 , C12P7/18 , C12R1/19
CPC分类号： C12N9/0006 , C12P7/18 , C12R1/19 , C12Y101/01 , C12Y101/01077 , C12Y101/01283 , C12Y208/03017 , C12Y402/03003 , C12Y404/01005 , C12Y501/02001
摘要： The present invention relates to new lactaldehyde reductase (LAR) enzymes useful for the production of 1,2-propane-diol and to microorganisms overexpressing said enzymes. The invention also relates to a method for producing 1,2-propanediol by converting lactaldehyde into 1,2-propanediol with said enzymes.

2. 发明申请

US20120122169A1 MUTANT YQHD ENZYME FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION 有权
标题翻译：通过发酵生产生物化学品的突变YQHD酶
公开(公告)号：US20120122169A1
公开(公告)日：2012-05-17
申请号：US13387347
申请日：2010-07-30
申请人： Francois Voelker , Laurence Dumon-Seignovert , Philippe Soucaille
发明人： Francois Voelker , Laurence Dumon-Seignovert , Philippe Soucaille
IPC分类号： C12P7/18 , C12N1/19 , C12N15/53 , C12N1/21 , C12P7/26 , C12N9/02
CPC分类号： C12P7/26 , C12N9/0006 , C12N9/88 , C12P7/18 , C12Y101/01002 , C12Y101/01006 , C12Y402/03003
摘要： The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased.The present invention also relates to a mutant YqhD enzyme comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the YqhD activity of the parent enzyme and the catalytic efficiency toward NADPH of the mutant YqhD is increased as compared with the catalytic efficiency toward NADPH of the parent enzyme.
摘要翻译：本发明涉及一种选自丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇的生物化学生产方法，该方法包括培养一种改良生物化学生物的微生物，丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇，并回收可以进一步纯化的所需生化物，其中微生物表达YqhD酶，其对NADPH的催化效率增加了。本发明还涉及包含在相同位置的不同氨基酸残基替代的母体酶的蛋白质序列中的至少一个氨基酸残基的突变型YqhD酶，其中突变酶保留了超过50％的YqhD活性的亲代酶的催化效率和与NADPH的亲和酶的催化效率相比，突变型YqhD的NADPH的催化效率增加。

3. 发明申请

US20190233848A1 METHODS AND MATERIALS FOR THE BIOSYNTHESIS OF DIOL ALCOHOLS AND RELATED COMPOUNDS 审中-公开
公开(公告)号：US20190233848A1
公开(公告)日：2019-08-01
申请号：US16264768
申请日：2019-02-01
申请人： INVISTA NORTH AMERICA S.A.R.L.
发明人： Nadia Fatma KADI , Daniel BAWDON , Alexander Brett FOSTER
IPC分类号： C12P7/18 , C12N15/74 , C12N9/04 , C12N9/88 , C12N9/12
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/1205 , C12N9/88 , C12N15/74 , C12Y101/01006 , C12Y101/01077 , C12Y101/01283 , C12Y207/01029 , C12Y402/03003
摘要： Methods and materials for the production of diol alcohols, such as 1,2-propanediol (1,2-PD) and derivatives and compounds related thereto. Also provided are products produced in accordance with these methods and materials.

4. 发明申请

US20150247174A1 ENGINEERING BACTERIA FOR PRODUCING DL-ALANINE AND METHOD FOR PRODUCING DL-ALANINE BY USING ENGINEERING BACTERIA 审中-公开
标题翻译：用于生产DL-ALANINE的工程细菌和使用工程细菌生产DL-ALANINE的方法
公开(公告)号：US20150247174A1
公开(公告)日：2015-09-03
申请号：US14395187
申请日：2012-12-28
申请人： ANHUI HUAHENG BIOENGINEERING CO., LTD.
发明人： Xueli Zhang , Dongzhu Zhang
IPC分类号： C12P13/06 , C12N15/70 , C12N9/90 , C12R1/19 , C12N9/06
CPC分类号： C12P13/06 , C12N9/0006 , C12N9/001 , C12N9/0016 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N15/70 , C12R1/19 , C12Y101/01001 , C12Y101/01027 , C12Y103/05001 , C12Y104/01001 , C12Y203/01054 , C12Y207/02001 , C12Y402/03003 , C12Y501/01001
摘要： The present invention discloses a DL-alanine-producing engineering bacterium. This DL-alanine-producing engineering bacterium itself was inactivated in lactate dehydrogenase, pyruvate formate lyase, alcohol dehydrogenase, acetate kinase, fumarate reductase, alanine racemase, and methylglyoxal synthase; moreover, onto the chromosome thereof an exogenous L-alanine dehydrogenase gene and alanine racemase gene were integrated. In the present application, by integrating the exogenous L-alanine dehydrogenase gene into the chromosome of the engineering bacterium, the intermediate of glycolysis, pyruvic acid, was converted into L-alanine; by further integrating the exogenous alanine racemase gene, partial L-alanine was converted into D-alanine, achieving the direct production of DL-alanine from raw material saccharides, thereby decreasing the production cycle of DL-alanine, and increasing the yield of DL-alanine.
摘要翻译：本发明公开了一种DL-丙氨酸生产工程菌。这种DL-丙氨酸生产工程细菌本身在乳酸脱氢酶，丙酮酸甲酯裂解酶，醇脱氢酶，乙酸激酶，富马酸还原酶，丙氨酸消旋酶和甲基乙二醛合酶中失活; 此外，在其染色体上整合外源L-丙氨酸脱氢酶基因和丙氨酸消旋酶基因。在本申请中，通过将外源L-丙氨酸脱氢酶基因整合到工程细菌的染色体中，将糖酵解中间体丙酮酸转化为L-丙氨酸; 通过进一步整合外源性丙氨酸消旋酶基因，将部分L-丙氨酸转化为D-丙氨酸，实现从原料糖直接生产DL-丙氨酸，从而降低DL-丙氨酸的生产周期，提高DL-丙氨酸的产量，丙氨酸。

5. 发明授权

US08969053B2 Mutant YqhD enzyme for the production of a biochemical by fermentation 有权
标题翻译：用于通过发酵生产生化的突变型YqhD酶
公开(公告)号：US08969053B2
公开(公告)日：2015-03-03
申请号：US13387347
申请日：2010-07-30
申请人： François Voelker , Laurence Dumon-Seignovert , Philippe Soucaille
发明人： François Voelker , Laurence Dumon-Seignovert , Philippe Soucaille
IPC分类号： C12P7/26 , C12P1/00 , C12N9/04 , C12N9/88 , C12P7/18
CPC分类号： C12P7/26 , C12N9/0006 , C12N9/88 , C12P7/18 , C12Y101/01002 , C12Y101/01006 , C12Y402/03003
摘要： The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased.The present invention also relates to a mutant YqhD enzyme comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the YqhD activity of the parent enzyme and the catalytic efficiency toward NADPH of the mutant YqhD is increased as compared with the catalytic efficiency toward NADPH of the parent enzyme.
摘要翻译：本发明涉及一种选自丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇的生物化学生产方法，该方法包括培养一种改良生物化学生物的微生物，丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇，并回收可以进一步纯化的所需生化物，其中微生物表达YqhD酶，其对NADPH的催化效率增加了。本发明还涉及包含在相同位置的不同氨基酸残基替代的母体酶的蛋白质序列中的至少一个氨基酸残基的突变型YqhD酶，其中突变酶保留了超过50％的YqhD活性的亲代酶的催化效率和与NADPH的亲和酶的催化效率相比，突变型YqhD的NADPH的催化效率增加。

6. 发明申请

US20140113342A1 Production of 1,2-Propanediol in Cyanobacteria 审中-公开
标题翻译：在蓝细菌中生产1,2-丙二醇
公开(公告)号：US20140113342A1
公开(公告)日：2014-04-24
申请号：US14057014
申请日：2013-10-18
申请人： Algenol Biofuels Inc.
发明人： Karl ZIEGLER , Christian WEISSERT , Ulf DUEHRING , Jonathan Wong CHIN , Matthew Alexander ANDERSON , Jianping CUI , Matt SPIEKER
IPC分类号： C12P7/18
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/88 , C12N15/52 , C12Y101/01001 , C12Y101/01006 , C12Y101/01077 , C12Y402/03003
摘要： Cyanobacterial host cells are modified to produce 1,2-propanediol.
摘要翻译：蓝藻细菌宿主细胞被修饰以产生1,2-丙二醇。

7. 发明授权

EP3371204B1 GENETICALLY MODIFIED MICROORGANISMS 有权
公开(公告)号：EP3371204B1
公开(公告)日：2024-05-08
申请号：EP16794001.4
申请日：2016-11-04
IPC分类号： C12N9/12 , C12N9/88 , C12N15/52 , C12N15/62 , C12P1/04 , C12P7/18 , C12N1/20 , C12N9/04 , C07K14/195
CPC分类号： C07K14/195 , C12N1/20 , C07K2319/0120130101 , C12N9/0006 , C12N9/12 , C12N9/88 , C12N15/52 , C12N15/62 , C12P1/04 , C12P7/18 , C12Y101/01006 , C12Y101/01077 , C12Y207/01029 , C12Y402/03003

8. 发明申请

WO2014100920A1 生产DL-丙氨酸的工程菌及利用该工程菌生产DL-丙氨酸的方法审中-公开
公开(公告)号：WO2014100920A1
公开(公告)日：2014-07-03
申请号：PCT/CN2012/001760
申请日：2012-12-28
申请人：安徽华恒生物工程有限公司 , 张学礼 , 张冬竹
发明人：张学礼 , 张冬竹
IPC分类号： C12N1/21 , C12P13/06 , C12R1/19
CPC分类号： C12P13/06 , C12N9/0006 , C12N9/001 , C12N9/0016 , C12N9/1029 , C12N9/1217 , C12N9/88 , C12N9/90 , C12N15/70 , C12R1/19 , C12Y101/01001 , C12Y101/01027 , C12Y103/05001 , C12Y104/01001 , C12Y203/01054 , C12Y207/02001 , C12Y402/03003 , C12Y501/01001
摘要：提供一种生产DL-丙氨酸的工程菌，该工程菌自身的乳酸脱氢酶、丙酮酸甲酸裂解酶、乙醇脱氢酶、乙酸激酶、富马酸还原酶、丙氨酸消旋酶、甲基乙二酸合成酶均被失活；且其染色体上整合了外源的L-丙氨酸脱氢酶基因和丙氨酸消旋酶基因。通过在工程菌染色体上整合了外源的L-丙氨酸脱氢酶基因，从而将糖酵解的中间物丙酮酸转化为L-丙氨酸；再进一步的整合外源丙氨酸消旋酶基因，将部分L-丙氨酸转化为D-丙氨酸，从而实现由糖原料一步生产DL-丙氨酸，减少DL-丙氨酸的生产周期，且可提高DL-丙氨酸的产率。

9. 发明申请

WO2011012697A3 MUTANT YQHD ENZYME FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION 审中-公开
标题翻译：通过发酵生产生物化学品的突变YQHD酶
公开(公告)号：WO2011012697A3
公开(公告)日：2011-05-19
申请号：PCT/EP2010061106
申请日：2010-07-30
申请人： METABOLIC EXPLORER SA , VOELKER FRANCOIS , DUMON-SEIGNOVERT LAURENCE , SOUCAILLE PHILIPPE
发明人： VOELKER FRANCOIS , DUMON-SEIGNOVERT LAURENCE , SOUCAILLE PHILIPPE
IPC分类号： C12N9/02 , C12P7/18 , C12P7/26
CPC分类号： C12P7/26 , C12N9/0006 , C12N9/88 , C12P7/18 , C12Y101/01002 , C12Y101/01006 , C12Y402/03003
摘要： The present invention concerns a method for the production of a biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol comprising culturing a microorganism modified for an improved production of the biochemical selected among acetol and 1,2-propanediol, 1,3-propanediol, ethylene glycol and 1,4-butanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a YqhD enzyme which catalytic efficiency toward NADPH is increased. The present invention also relates to a mutant YqhD enzyme comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the YqhD activity of the parent enzyme and the catalytic efficiency toward NADPH of the mutant YqhD is increased as compared with the catalytic efficiency toward NADPH of the parent enzyme.
摘要翻译：本发明涉及生产选自丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇的生物化学方法，该方法包括培养一种改良生物化学生产的微生物，其选自丙酮醇和1,2-丙二醇，1,3-丙二醇，乙二醇和1,4-丁二醇，并回收可以进一步纯化的所需生物化学物质，其中微生物表达YqhD酶，其对NADPH的催化效率增加了。本发明还涉及包含在相同位置的不同氨基酸残基替换的母体酶的蛋白质序列中的至少一个氨基酸残基的突变型YqhD酶，其中突变酶保留超过YqhD活性的50％的亲代酶的催化效率和与NADPH的亲和酶的催化效率相比，突变体YqhD的NADPH的催化效率增加。

10. 发明授权

EP2459711B1 Mutant YqhD enzyme for the production of a biochemical by fermentation 有权
标题翻译：对于通过发酵生产的生物化学突变体酶的yqhD
公开(公告)号：EP2459711B1
公开(公告)日：2016-04-06
申请号：EP10737910.9
申请日：2010-07-30
申请人： Metabolic Explorer
发明人： VOELKER, François , DUMON-SEIGNOVERT, Laurence , SOUCAILLE, Philippe
IPC分类号： C12N9/04 , C12N9/88 , C12P7/18 , C12P7/26
CPC分类号： C12P7/26 , C12N9/0006 , C12N9/88 , C12P7/18 , C12Y101/01002 , C12Y101/01006 , C12Y402/03003

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