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    • 5. 发明申请
    • STABLE INDICATOR SOLUTIONS FOR DETECTION OF PEROXIDATIC ACTIVITY
    • 用于检测过氧化活性的稳定指示剂溶液
    • WO1990002339A1
    • 1990-03-08
    • PCT/US1988002806
    • 1988-08-16
    • CETUS CORPORATIONBLOCH, Will
    • CETUS CORPORATION
    • G01N33/72
    • C12Q1/28C12Q2326/12C12Q2326/20G01N33/725
    • Disclosed are (a) aqueous indicator solutions useful for detection and quantitation of peroxidatic activity, containing a hydroperoxide and a chromogenic organic electron donor of the benzidine family, which possess the novel property of developing negligible color and maintaining approximately full sensitivity during storage at 0-40 DEG C on a time scale minimally of six months; (b) indicator solutions for peroxidatic activity wherein the chromogen is o-phenylenediamine and storage stability is minimally for one to two days at room temperature or two weeks at 5 DEG C; (c) certain chelators which promote these storage stability goals for o-phenylenediamine and for the benzidine compounds; and (d) processes for preparing such indicator solutions, comprising measures for minimizing contamination by oxidatively active transition metals. The resulting solutions increase the simplicity and reliability of a wide array of chemical, biological, and clinical tests, where either the analyte is a peroxidatic catalyst or the analyte is detected through the specific binding of another molecule, linked directly or indirectly to a peroxidatic catalyst.
    • 公开了(a)可用于检测和定量过氧化活性的含水指示剂溶液,其含有联苯胺族的氢过氧化物和显色有机电子给体,其具有在0-储存期间发展可忽略的颜色并保持近似全灵敏度的新特性, 40℃,最短时间为6个月; (b)过氧化活性的指示剂溶液,其中色原是邻苯二胺,储存稳定性在室温或5℃下最低限度为1至2天; (c)促进邻苯二胺和联苯胺化合物的这些储存稳定性目标的某些螯合剂; 和(d)制备这种指示剂溶液的方法,包括使氧化活性过渡金属的污染最小化的措施。 所得到的解决方案增加了广泛的化学,生物和临床试验的简单性和可靠性,其中分析物是过氧化物催化剂,或者通过另一分子的特异性结合检测分析物,直接或间接连接到过氧化催化剂 。