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    • 9. 发明申请
    • POST-PARTUM MAMMALIAN PLACENTA, ITS USE AND PLACENTAL STEM CELLS THEREFROM
    • 后处理药物,其使用和其他细胞干细胞
    • WO2002064755A2
    • 2002-08-22
    • PCT/US2002/004282
    • 2002-02-13
    • ANTHROGENESIS CORPORATION
    • HARIRI, Robert, J.
    • C12N5/06
    • C12N5/0605A61K35/12A61K48/00A61M1/02A61M2202/0462A61M2210/1458A61M2210/1466C12N5/0068C12N2500/90C12N2509/00C12N2533/90
    • The present invention provides a method of extracting and recovering embryonic like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to move residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.
    • 本发明提供了从放血的人胎盘中提取和回收胚胎样干细胞(包括但不限于多潜能或多能干细胞)的方法。 通过灌注放血的胎盘,优选用抗凝血剂溶液来处理胎盘以移动残留的脐带血以冲洗残留的细胞。 收集来自放血胎盘的残留细胞和灌注液,从残留细胞和灌注液中分离胚胎样干细胞。 本发明还提供了利用分离和灌注的胎盘作为其中传播内源性细胞的生物反应器的方法,其包括但不限于胚胎样干细胞。 本发明还提供了用于在胎盘生物反应器中繁殖外源细胞并从其收集繁殖的外源细胞和生物活性分子的方法。
    • 10. 发明申请
    • UMBILICAL CORD BLOOD COLLECTION DEVICE AND METHOD
    • 脐带血液收集装置及方法
    • WO1998008433A1
    • 1998-03-05
    • PCT/US1997014988
    • 1997-08-26
    • GRUENBERG, Lisa, J.
    • A61B05/00
    • A61B5/417A61B5/150038A61B5/150389A61B5/150748A61B17/42A61M1/02A61M2210/1466
    • An umbilical cord blood collection device (10) includes a base (12) defining a collection surface (16) for receiving and supporting a segment of an umbilical cord (C), in communication with a placenta, from which blood is to be collected. The base, in at least a region including the collection surface, is resistant to penetration by a blood collection needle. A clamp (20) is mounted for clamping engagement across the umbilical cord supported upon the collection surface. A securement strap (26) is disposed for securing engagement with the umbilical cord. A malleable arm (30) has a first end mounted to the base and a second, free end supporting a blood collection needle (28). A method for collection of umbilical cord blood is also described.
    • 脐带血采集装置(10)包括限定收集表面(16)的基部(12),用于接收和支撑与待收集血液的胎盘相连通的脐带(C)的一段。 在至少包括收集表面的区域中的基底抵抗采血针的穿透。 夹具(20)被安装用于夹紧在支撑在收集表面上的脐带的接合。 设置固定带(26)以固定与脐带的接合。 可延展的臂(30)具有安装到基部的第一端和支撑采血针(28)的第二自由端。 还描述了收集脐带血的方法。