专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP6276780B2 除草剤抵抗性のテンサイ植物を生育する方法有权
公开(公告)号：JP6276780B2
公开(公告)日：2018-02-07
申请号：JP2015547057
申请日：2013-12-13
申请人：セスヴァンダーハーブ・エヌ・ブイ , SESVanderHave N.V.
发明人：ヴェインズ,ギー , ルフェーヴル,マルク , ハイン,リューディガー , ヨハン,ゲルハルト
IPC分类号： A01H5/00 , C12N15/01 , C12N5/04 , A01H6/02 , A01N43/54 , A01H1/06
CPC分类号： A01H4/008 , A01H3/04 , A01H4/00 , C12N15/8274 , C12N15/8278

2. 发明申请

WO2014091021A1 METHOD TO DEVELOP HERBICIDE-RESISTANT SUGAR BEET PLANTS 审中-公开
标题翻译：研制抗除草剂糖果植物的方法
公开(公告)号：WO2014091021A1
公开(公告)日：2014-06-19
申请号：PCT/EP2013/076618
申请日：2013-12-13
申请人： SESVANDERHAVE N.V.
发明人： WEYENS, Guy , LEFÈBVRE, Marc , HAIN, Rüdiger , JOHANN, Gerhard
IPC分类号： C12N15/82 , A01H3/04 , A01H4/00 , A01H5/06
CPC分类号： A01H4/008 , A01H3/04 , A01H4/00 , C12N15/8274 , C12N15/8278
摘要： A method for producing herbicide-resistant sugar beet plant comprising the steps of: -obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant; -applying to the cellsa composition comprising an ALS herbicide at a concentration which is lethal to the said cells; and -regenerating sugar beet plants from the surviving cells.
摘要翻译：一种抗除草剂甜菜植物的制备方法，包括以下步骤： - 从甜菜植物分离的气孔保护细胞中获得原生质体; 将包含ALS除草剂的组合物施用于细胞，所述组合物以对所述细胞致死的浓度; 并从存活细胞中重新产生甜菜植物。

3. 发明授权

US10517244B2 Method to develop herbicide-resistant sugar beet plants 有权
公开(公告)号：US10517244B2
公开(公告)日：2019-12-31
申请号：US14443587
申请日：2013-12-13
申请人： SESVanderHave N.V.
发明人： Guy Weyens , Marc Lefebvre , Rudiger Hain , Gerhard Johann
IPC分类号： A01H4/00 , C12N15/82 , A01H3/04
摘要： A method for producing herbicide-resistant sugar beet plant includes obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant. A composition having an ALS herbicide is applied to the cells at a concentration which is lethal to the cells. Sugar beet plants are regenerated from the surviving cells.

4. 发明授权

US07663024B2 P15 hairpin constructs and use 有权
标题翻译： P15发夹构建和使用
公开(公告)号：US07663024B2
公开(公告)日：2010-02-16
申请号：US11418384
申请日：2006-05-03
申请人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard , Elodie Klein , David Gilmer
发明人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard , Elodie Klein , David Gilmer
IPC分类号： A01H1/00 , C12N15/82 , C12N15/87 , C07H21/04
CPC分类号： C12N15/8283
摘要： The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.
摘要翻译：本发明涉及TGB-3野生型病毒序列的遗传修饰方法，用于降低或抑制所述TGB-3病毒序列对转化的植物或植物细胞的农学性质的可能有害影响。本发明还涉及适于诱导基因沉默的经遗传修饰的TGB-3病毒序列。特别地，基于这样的序列的发夹构建体被证明是高效诱导PTGS机制并由此降解整个RNA2。当植物相应地转化时，病毒在植物中的扩散显着减少或阻断。

5. 发明申请

WO2007128755A3 P15 HAIRPIN CONSTRUCTS AND USE 审中-公开
公开(公告)号：WO2007128755A3
公开(公告)日：2007-11-15
申请号：PCT/EP2007/054259
申请日：2007-05-02
申请人： SESVANDERHAVE N.V. , LAUBER, Emmanuelle , GUILLEY, Hubert , RICHARDS, Ken , JONARD, Gerard , KLEIN, Elodie , GILMER, David
发明人： LAUBER, Emmanuelle , GUILLEY, Hubert , RICHARDS, Ken , JONARD, Gerard , KLEIN, Elodie , GILMER, David
IPC分类号： C07K14/08 , C12N15/82
摘要： The invention relates to genetically modified TGB- 3 viral sequences suitable to induce gene silencing. In particular, hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly, the spread of the virus in the plant is significantly reduced or blocked. The invention relates of the hairpin constructs, their use and to plants, plant cells and plant tissues harboring these constructs.

6. 发明申请

WO2015189376A1 USE OF A SELECTABLE MARKER GENE IN SUGAR BEET PROTOPLASTS TRANSFORMATION METHOD AND SYSTEM 审中-公开
标题翻译：使用可选择的标记基因进行糖酵母转化方法和系统
公开(公告)号：WO2015189376A1
公开(公告)日：2015-12-17
申请号：PCT/EP2015/063144
申请日：2015-06-12
申请人： SESVANDERHAVE N.V.
发明人： WEYENS, Guy , LEFÈBVRE, Marc , THAMMANNAGOWDA, Shivegowda , LOMMEL, Murielle
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/821 , A01H1/02 , A01H1/04 , C12N9/88 , C12N15/52 , C12N15/8209 , C12N15/8241 , C12N15/8279 , C12N15/8281 , C12N15/8282 , C12N15/8285 , C12N15/8286
摘要： The present invention is related to a method for the transformation of sugar beet protoplasts comprising the steps of: – obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant, - transforming the protoplasts with a nucleic acid construct comprising a nucleotide sequence of interest and a selection marker sequence, - applying to an in vitro culture of said protoplasts, one or more ALS inhibitors at a concentration that is lethal to the in vitro culture of the protoplasts and - regenerating sugar beet plants from the surviving protoplasts having integrated the nucleic acid construct comprising the sequence of interest and the selection marker sequence, wherein the selectionmarker sequence is the mutated BvALS113 sequence carrying in its sequence a mutation at amino acid 113 position from Alanine to Tyrosine.
摘要翻译：本发明涉及一种用于转化甜菜原生质体的方法，包括以下步骤： - 从分离自甜菜植物的气孔保护细胞获得原生质体， - 用包含感兴趣的核苷酸序列的核酸构建体转化原生质体，以及选择标记序列， - 以对原生质体的体外培养致死的浓度应用所述原生质体的一种或多种ALS抑制剂的体外培养，以及从具有整合核酸的存活的原生质体再生甜菜植物构建体，其包含感兴趣的序列和选择标记序列，其中选择标记序列是突变的BvALS113序列，其在氨基酸113位从氨基酸到酪氨酸的突变。

7. 发明申请

US20090265808A1 P15 HAIRPIN CONSTRUCTS AND USE 审中-公开
标题翻译： P15发型建议和使用
公开(公告)号：US20090265808A1
公开(公告)日：2009-10-22
申请号：US12425331
申请日：2009-04-16
申请人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard , Elodie Klein , David Gilmer
发明人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard , Elodie Klein , David Gilmer
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04 , C07H21/02 , C12N15/63
CPC分类号： C12N15/8283
摘要： The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.
摘要翻译：本发明涉及TGB-3野生型病毒序列的遗传修饰方法，用于降低或抑制所述TGB-3病毒序列对转化的植物或植物细胞的农学性质的可能有害影响。本发明还涉及适于诱导基因沉默的经遗传修饰的TGB-3病毒序列。特别地，基于这样的序列的发夹构建体被证明是高效诱导PTGS机制并由此降解整个RNA2。当植物相应地转化时，病毒在植物中的扩散显着减少或阻断。

8. 发明申请

WO2015189409A1 TRANSFORMATION METHOD OF SUGAR BEET PROTOPLASTS BY TALEN PLATFORM TECHNOLOGY 审中-公开
标题翻译：通过TALEN PLATFORM技术进行糖果甜菜的转化方法
公开(公告)号：WO2015189409A1
公开(公告)日：2015-12-17
申请号：PCT/EP2015/063216
申请日：2015-06-12
申请人： SESVANDERHAVE N.V.
发明人： WEYENS, Guy , LEFÈBVRE, Marc , THAMMANNAGOWDA, Shivegowda , LOMMEL, Murielle
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8213 , C12N9/88
摘要： Method for the transformation of sugar beet protoplasts comprising the step of: –obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant, -transforming said protoplasts with a nucleic acid construct comprising a nucleotide sequence of interest and Transcription Activator-Like Effector Nucleases (TALEN) or one or more vectors comprising sequences encoding these Transcription Activator-Like Effector Nucleases (TALEN)sequences, wherein these TALEN target and process a target sequence and replace this target sequence through homologous recombination with the nucleic acid construct comprising the nucleotide sequence of interest, -possibly applying to an in vitroculture of these protoplasts, a medium that is toxic, preferably lethal to the in vitroculture of the protoplasts, and -regenerating sugar beet plants from the cell culture, preferably from the surviving protoplasts having integrated the nucleic acid construct comprising the sequence of interest that possibly renders the transformed cell resistant to the toxic activity of the applied medium.
摘要翻译：用于转化糖用甜菜原生质体的方法，包括以下步骤： - 从分离自甜菜植物的气孔保护细胞获得原生质体， - 用包含感兴趣的核苷酸序列和转录激活子样效应核酸酶的核酸构建体转化所述原生质体（ TALEN）或包含编码这些转录激活子样效应核酸酶（TALEN）序列的序列的一种或多种载体，其中这些TALEN靶标并通过与包含感兴趣的核苷酸序列的核酸构建体的同源重组来处理靶序列并替换该靶序列可能地应用于这些原生质体的维生素培养物中，这种培养基是有毒的，优选对原生质体的维生素培养是致死的，并且来自细胞培养物的再生甜菜植物，优选来自已经整合了核酸构建体的存活的原生质体包括可能的兴趣序列使得转化细胞对所施用的培养基的毒性活性具有抗性。

9. 发明申请

WO2015188870A1 USE OF SELECTABLE MARKER GENE IN SUGAR BEET PROTOPLASTS TRANSFORMATION METHOD AND SYSTEM 审中-公开
标题翻译：使用可选择的标记基因进行糖酵母转化方法和系统
公开(公告)号：WO2015188870A1
公开(公告)日：2015-12-17
申请号：PCT/EP2014/062291
申请日：2014-06-12
申请人： SESVANDERHAVE N.V.
发明人： WEYENS, Guy , LEFÈBVRE, Marc , THAMMANNAGOWDA, Shivegowda , LOMMEL, Murielle
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8201 , C07K14/415 , C12N15/8278
摘要： The present invention is related to a method for the transformation of sugar beet protoplasts comprising the step of: – obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant, - transforming said protoplasts with a nucleic acid construct comprising a nucleotide sequence of interest and a marker sequence, - applying to an in vitro culture of said protoplasts, one or more ALS inhibitors at a concentration that is lethal to the in vitro culture of the protoplasts and - regenerating sugar beet plants from the surviving protoplasts having integrated the nucleic acid construct comprising the sequence of interest and the selectable marker sequence where the selectable marker sequence is the mutated BvALS113 sequence carrying in its sequence a mutation at amino acid 113 position from Alanine to Tyrosine.
摘要翻译：本发明涉及一种用于转化甜菜原生质体的方法，包括以下步骤： - 从分离自甜菜植物的气孔保护细胞获得原生质体， - 用包含感兴趣的核苷酸序列的核酸构建体转化所述原生质体，以及标记序列， - 应用于所述原生质体的体外培养物，一种或多种ALS抑制剂，其浓度对于原生质体的体外培养是致命的，并且从具有整合核酸构建体的存活原生质体再生甜菜植物包括感兴趣的序列和选择性标记序列，其中选择标记序列是突变的BvALS113序列，其携带从氨基酸113位置到丙氨酸到酪氨酸的突变。

10. 发明公开

EP3473645A1 GENE FOR YIELD INCREASE 审中-公开
公开(公告)号：EP3473645A1
公开(公告)日：2019-04-24
申请号：EP17197076.7
申请日：2017-10-18
申请人： SESVanderHave N.V. , Michigan Technological University
发明人： Busov, Victor
IPC分类号： C07K14/415 , C12N15/82
摘要： A DNA vector suitable for the expression in an host cell of SEQ.ID.NO:2, the corresponding host cell and transgenic plant, and a method for the identification or the generation of plants having increased nitrogen use efficiency.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式