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1. 发明申请

US20090265808A1 P15 HAIRPIN CONSTRUCTS AND USE 审中-公开
标题翻译： P15发型建议和使用
公开(公告)号：US20090265808A1
公开(公告)日：2009-10-22
申请号：US12425331
申请日：2009-04-16
申请人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard , Elodie Klein , David Gilmer
发明人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard , Elodie Klein , David Gilmer
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04 , C07H21/02 , C12N15/63
CPC分类号： C12N15/8283
摘要： The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.
摘要翻译：本发明涉及TGB-3野生型病毒序列的遗传修饰方法，用于降低或抑制所述TGB-3病毒序列对转化的植物或植物细胞的农学性质的可能有害影响。本发明还涉及适于诱导基因沉默的经遗传修饰的TGB-3病毒序列。特别地，基于这样的序列的发夹构建体被证明是高效诱导PTGS机制并由此降解整个RNA2。当植物相应地转化时，病毒在植物中的扩散显着减少或阻断。

2. 发明授权

US07663024B2 P15 hairpin constructs and use 有权
标题翻译： P15发夹构建和使用
公开(公告)号：US07663024B2
公开(公告)日：2010-02-16
申请号：US11418384
申请日：2006-05-03
申请人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard , Elodie Klein , David Gilmer
发明人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard , Elodie Klein , David Gilmer
IPC分类号： A01H1/00 , C12N15/82 , C12N15/87 , C07H21/04
CPC分类号： C12N15/8283
摘要： The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.
摘要翻译：本发明涉及TGB-3野生型病毒序列的遗传修饰方法，用于降低或抑制所述TGB-3病毒序列对转化的植物或植物细胞的农学性质的可能有害影响。本发明还涉及适于诱导基因沉默的经遗传修饰的TGB-3病毒序列。特别地，基于这样的序列的发夹构建体被证明是高效诱导PTGS机制并由此降解整个RNA2。当植物相应地转化时，病毒在植物中的扩散显着减少或阻断。

3. 发明申请

US20060288445A1 P15 hairpin constructs and use 有权
标题翻译： P15发夹构建和使用
公开(公告)号：US20060288445A1
公开(公告)日：2006-12-21
申请号：US11418384
申请日：2006-05-03
申请人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard
发明人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard
IPC分类号： A01H1/00 , C12N7/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8283
摘要： The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence, comprising the following successive steps: submitting said sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid; selecting genetically modified TGB-3 wild type viral sequences having said point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon; further selecting among said genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon, and recovering said specifically genetically modified TGB-3 viral sequence. The invention further relates to genetically modified TGB-3 viral sequences suitable to induce gene silencing. In particular hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly the spread of the virus in the plant is significantly reduced or blocked.
摘要翻译：本发明涉及一种TGB-3野生型病毒序列的遗传修饰方法，用于降低或抑制所述TGB-3病毒序列对转化的植物或植物细胞的农学性质的可能有害影响，包括以下连续步骤：将所述序列提交至允许将至少一个氨基酸置换成不同氨基酸的点突变; 选择具有所述点突变的遗传修饰的TGB-3野生型病毒序列，并且不能促进具有功能异常的TGB-3野生型病毒序列的突变病毒的细胞间移动，当以复制品所述遗传修饰的TGB-3病毒序列，当突变形式由复制子表达时，特异性遗传修饰的序列，其抑制用共同接种的野生型病毒感染，并回收所述特异性遗传修饰的TGB-3病毒序列。本发明还涉及适于诱导基因沉默的经遗传修饰的TGB-3病毒序列。特别地，基于这样的序列的发夹构建体被证明是高效诱导PTGS机制并由此降解整个RNA2。当植物相应地转化时，病毒在植物中的扩散显着减少或阻断。

4. 发明申请

US20050138687A1 Method of genetic modification of a wild type viral sequence 审中-公开
标题翻译：野生型病毒序列的遗传修饰方法
公开(公告)号：US20050138687A1
公开(公告)日：2005-06-23
申请号：US10980146
申请日：2004-11-01
申请人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard
发明人： Emmanuelle Lauber , Hubert Guilley , Ken Richards , Gerard Jonard
IPC分类号： A01H5/00 , C07K14/08 , C12N5/10 , C12N15/82 , C12Q1/68 , C12Q1/70 , A01H1/00
CPC分类号： C07K14/005 , C12N15/8283 , C12N2770/00022
摘要： The present invention concerns a method of genetic modification of a TGB-3 wild type viral sequence for reducing or suppressing the possible deleterious effects of the agronomic properties of a transformed plant or plant cell by said TGB-3 viral sequence, comprising the following successive steps: submitting said sequence to point mutation(s) which allow the substitution of at least one amino-acid into a different amino-acid; selecting genetically modified TGB-3 wild type viral sequences having said point mutation(s) and which are not able to promote cell-to-cell movement of a mutant virus having a dysfunctional TGB-3 wild type viral sequence, when expressed in trans from a replicon; further selecting among said genetically modified TGB-3 viral sequences, the specifically genetically modified sequence which inhibits infection with a co-inoculated wild type virus when the mutant form was expressed from a replicon; and recovering said specifically genetically modified TGB-3 viral sequence.
摘要翻译：本发明涉及一种TGB-3野生型病毒序列的遗传修饰方法，用于降低或抑制所述TGB-3病毒序列对转化的植物或植物细胞的农学性质的可能有害影响，包括以下连续步骤：将所述序列提交至允许将至少一个氨基酸置换成不同氨基酸的点突变; 选择具有所述点突变的遗传修饰的TGB-3野生型病毒序列，并且不能促进具有功能异常的TGB-3野生型病毒序列的突变病毒的细胞间移动，当以复制品所述遗传修饰的TGB-3病毒序列进一步选择，当突变形式由复制子表达时，特异性遗传修饰的序列可抑制共同接种的野生型病毒感染; 并回收所述特异性遗传修饰的TGB-3病毒序列。

5. 发明授权

US06297428B1 Method for inducing viral resistance into a plant 有权
标题翻译：诱导病毒抗性进入植物的方法
公开(公告)号：US06297428B1
公开(公告)日：2001-10-02
申请号：US09242216
申请日：1999-02-08
申请人： Hubert Guilley , Gerard Jonard , Ken Richards , Salah Bouzoubaa , Claudine Bleykasten-Grosshans , Guy Weyens , Marc Lefebvre
发明人： Hubert Guilley , Gerard Jonard , Ken Richards , Salah Bouzoubaa , Claudine Bleykasten-Grosshans , Guy Weyens , Marc Lefebvre
IPC分类号： C12N504
CPC分类号： C07K14/005 , C12N15/8283 , C12N2770/40022
摘要： The present invention concerns a method for inducing resistance to a virus comprising a TGB3 sequence with the proviso that it is not the potato virus X, into a plant cell or plant, comprising the following steps: preparing a nucleic acid construct comprising a nucleic acid sequence corresponding to at least 70% of the nucleic acid sequence of TGB3 of said virus or its corresponding cDNA, being operably linked to one or more regulatory sequence(s) active in a plant, transforming a plant cell with the nucleic acid construct, and possibly regenerating a transgenic plant from the transformed plant cell. The present invention is also related to the plant obtained.
摘要翻译：本发明涉及一种诱导对病毒的抗性的方法，其包含TGB3序列，条件是不是马铃薯病毒X，其包括以下步骤：制备包含核酸序列的核酸构建体对应于所述病毒或其相应cDNA的TGB3的核酸序列的至少70％，其与植物中活性的一种或多种调节序列可操作地连接，用核酸构建体转化植物细胞，并且可能从转化的植物细胞再生转基因植物。本发明还涉及获得的植物。

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