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    • 6. 发明公开
    • CINERARIUM-DERIVED CHROMOSOMAL DNA INVOLVED IN SYNTHESIS OF FLAVONOID, AND USE THEREOF
    • 维生素B染色体大肠杆菌(CUSERARIEN ZUR FLAVONOIDSYNTHESE)
    • EP2345717A1
    • 2011-07-20
    • EP09823715.9
    • 2009-10-26
    • International Flower Developments Proprietary Limited
    • TANAKA, YoshikazuBRUGLIERA, Filippa
    • C12N15/00A01H5/00C12N1/15C12N1/19C12N1/21C12N15/09
    • C12N15/825C12N9/0073C12N15/8225
    • Disclosed are: a novel cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3',5'-hydroxylase (F3'5'H); and a novel F3'5'H gene construct which carries the polynucleotide. Specifically disclosed are: a cineraria-derived petal-specific promoter which is selected from the group consisting of (1) a polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 and (2) a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 under stringent conditions and can act as a promoter for a coding region of flavonoid 3',5'-hydroxylase (F3'5'H); and a gene construct for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the novel cineraria-derived polynucleotide.
    • 本发明涉及一种新颖的来源于线粒体的多核苷酸,可以作为黄酮3',5'-羟化酶(F3'5'H)编码区的启动子。 和携带多核苷酸的新颖的F3'5'H基因构建体。 具体公开的是:来自花粉衍生的花瓣特异性启动子,其选自(1)具有SEQ ID NO:9所示核苷酸序列的多核苷酸和(2)可与具有SEQ ID NO:9的多核苷酸杂交的多核苷酸 在严格条件下描述于SEQ ID NO:9的核苷酸序列,可以作为黄酮3',5'-羟化酶(F3'5'H)编码区的启动子。 以及用于以RNAi方法抑制基因表达的基因构建体,该基因构建体具有作为环的多核苷酸,该多核苷酸包含新的来源于多种核苷酸的多核苷酸的第一内含子的全部或部分。
    • 10. 发明公开
    • Genetic sequences having methyltransferase activity and uses therefor
    • Genetische Sequenzen mitMethyltransferaseaktivitätund Verwendungendafür
    • EP2096172A3
    • 2009-11-11
    • EP09004548.5
    • 2003-01-24
    • International Flower Developments Proprietary Limited
    • Brugliera, FilippaDemels, LindaTanaka, Yoshikazu
    • C12N15/29C12N15/11A01H5/00C07H21/04
    • C12N9/1007C12N15/825C12N15/827
    • The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and/or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S -adenosyl-L-methionine: anthocyanin 3' - O -methyltransferase or S- adenosyl-L-methionine: anthocyanin 3', 5'-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.
    • 本发明一般涉及编码具有甲基转移酶活性的多肽的遗传序列,以及遗传序列和/或多肽修饰植物一种或多种表型特征的用途。 更具体地,本发明的甲基转移酶作用于类黄酮,优选其中类黄酮是花青素。 更具体地说,本发明涉及具有S-腺苷-L-甲硫氨酸:花青素3'-O-甲基转移酶或S-腺苷-L-甲硫氨酸:花青素3',5'-O-甲基转移酶活性的多肽。 本发明还进一步提供了编码具有衍生自矮牵牛属,Torenia Fuchsia或Plumbago或植物相关植物的甲基转移酶活性的多肽的遗传序列。 本发明还涉及对应于全部或部分受试者遗传序列以及转基因植物以及来自这些植物的切花,部分,提取物和生殖组织的反义和有义分子。