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    • 6. 发明公开
    • CINERARIUM-DERIVED CHROMOSOMAL DNA INVOLVED IN SYNTHESIS OF FLAVONOID, AND USE THEREOF
    • 维生素B染色体大肠杆菌(CUSERARIEN ZUR FLAVONOIDSYNTHESE)
    • EP2345717A1
    • 2011-07-20
    • EP09823715.9
    • 2009-10-26
    • International Flower Developments Proprietary Limited
    • TANAKA, YoshikazuBRUGLIERA, Filippa
    • C12N15/00A01H5/00C12N1/15C12N1/19C12N1/21C12N15/09
    • C12N15/825C12N9/0073C12N15/8225
    • Disclosed are: a novel cineraria-derived polynucleotide which can act as a promoter for a coding region of flavonoid 3',5'-hydroxylase (F3'5'H); and a novel F3'5'H gene construct which carries the polynucleotide. Specifically disclosed are: a cineraria-derived petal-specific promoter which is selected from the group consisting of (1) a polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 and (2) a polynucleotide which can hybridize with the polynucleotide having the nucleotide sequence depicted in SEQ ID NO:9 under stringent conditions and can act as a promoter for a coding region of flavonoid 3',5'-hydroxylase (F3'5'H); and a gene construct for inhibiting the expression of a gene in an RNAi method, which has, as a loop, a polynucleotide comprising the whole or a part of a first intron of the novel cineraria-derived polynucleotide.
    • 本发明涉及一种新颖的来源于线粒体的多核苷酸,可以作为黄酮3',5'-羟化酶(F3'5'H)编码区的启动子。 和携带多核苷酸的新颖的F3'5'H基因构建体。 具体公开的是:来自花粉衍生的花瓣特异性启动子,其选自(1)具有SEQ ID NO:9所示核苷酸序列的多核苷酸和(2)可与具有SEQ ID NO:9的多核苷酸杂交的多核苷酸 在严格条件下描述于SEQ ID NO:9的核苷酸序列,可以作为黄酮3',5'-羟化酶(F3'5'H)编码区的启动子。 以及用于以RNAi方法抑制基因表达的基因构建体,该基因构建体具有作为环的多核苷酸,该多核苷酸包含新的来源于多种核苷酸的多核苷酸的第一内含子的全部或部分。