专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07323337B2 Gene transfer into primate embryonic stem cells using VSV-G pseudotyped simian immunodeficiency virus vectors 失效
标题翻译：使用VSV-G假型猿猴免疫缺陷病毒载体将基因转移到灵长类动物胚胎干细胞中
公开(公告)号：US07323337B2
公开(公告)日：2008-01-29
申请号：US10479912
申请日：2002-05-29
申请人： Yutaka Hanazono , Yasuji Ueda , Yasushi Kondo , Yutaka Suzuki
发明人： Yutaka Hanazono , Yasuji Ueda , Yasushi Kondo , Yutaka Suzuki
IPC分类号： C12N15/86 , C12N5/00 , C12N15/82 , C12N5/02
CPC分类号： G01N33/5073 , A01K2217/05 , A01K2227/105 , A01K2227/106 , A01K2267/025 , A01K2267/03 , A61K48/00 , C12N15/8509 , C12N15/86 , C12N2506/02 , C12N2510/00 , C12N2740/15043 , C12N2740/15045 , C12N2810/6081 , G01N33/5008 , G01N2333/22
摘要： Highly efficient gene transfer into primate-derived embryonic stem (ES) cells has successfully been achieved by using a simian immunodeficiency virus vector (SIV) pseudotyped with VSV-G protein, which is a surface glycoprotein of vesicular stomatitis virus (VSV) The present invention provides simian immunodeficiency virus vectors for gene transfer to primate ES cells. The method for gene transfer to primate ES cells using the vectors of the present invention is useful in, for example, research into embryology and disease, clinical applications, and experimental models for primates. The method is also useful in assaying and screening for genes and reagents able to enhance the specific differentiation of tissues or cells, and which are useful in preparing desired cells or tissues differentiated from ES cells.
摘要翻译：通过使用作为水泡性口炎病毒（VSV）的表面糖蛋白的VSV-G蛋白假型的猿免疫缺陷病毒载体（SIV），已经成功地实现了将高效率的基因转移到灵长类衍生的胚胎干细胞（ES）细胞中。本发明提供猿猴免疫缺陷病毒载体，用于基因转移到灵长类动物ES细胞。使用本发明的载体将基因转移到灵长类动物ES细胞的方法可用于例如研究胚胎学和疾病，临床应用和灵长类动物的实验模型。该方法还可用于测定和筛选能够增强组织或细胞的特异性分化的基因和试剂，并且其可用于制备从ES细胞分化的所需细胞或组织。

2. 发明申请

US20060257381A1 Method for transplanting lymphohematopoietic cells into mammal 审中-公开
标题翻译：将淋巴造血细胞移植到哺乳动物中的方法
公开(公告)号：US20060257381A1
公开(公告)日：2006-11-16
申请号：US10562322
申请日：2004-06-25
申请人： Keiya Ozawa , Yutaka Hanazono , Kyoji Ueda , Yasuji Ueda , Mamoru Hasegawa
发明人： Keiya Ozawa , Yutaka Hanazono , Kyoji Ueda , Yasuji Ueda , Mamoru Hasegawa
IPC分类号： A61K48/00 , C12N5/08
CPC分类号： A01K67/0271 , A61K48/0066 , A61K2035/124 , C07K14/505 , C07K14/715 , C07K14/7153 , C07K2319/00 , C12N5/0647 , C12N2799/027
摘要： The present invention provides a method for transplanting lymphohematopoietic cells into a mammal, which comprises the step of injecting cells into a bone marrow cavity, and wherein the cells have an exogenous gene encoding a receptor that induces cell proliferation in response to ligand binding. By combining intra-bone marrow transplantation (iBMT) and selective amplifier gene (SAG), marrow conditioning before the injection of the cells can be omitted. The present invention further provides a bone marrow transplant and a kit for transplanting lymphohematopoietic cells into mammals. Furthermore, the invention provides an SAG particularly suitable for such transplantation.
摘要翻译：本发明提供了将淋巴细胞生成细胞移植到哺乳动物中的方法，其包括将细胞注入骨髓腔中的步骤，并且其中细胞具有编码受体的外源基因，所述受体诱导响应于配体结合的细胞增殖。通过组合骨髓移植（iBMT）和选择性放大基因（SAG），可以省略注射细胞前的骨髓调节。本发明进一步提供骨髓移植和用于将淋巴造血细胞移植到哺乳动物中的试剂盒。此外，本发明提供了特别适合于这种移植的SAG。

3. 发明申请

US20110162093A1 METHODS FOR PRODUCING ANTIBODIES 审中-公开
标题翻译：生产抗体的方法
公开(公告)号：US20110162093A1
公开(公告)日：2011-06-30
申请号：US11922278
申请日：2006-06-13
申请人： Yasuji Ueda , Hiroto Hara , Tsugumine Shu , Mamoru Hasegawa
发明人： Yasuji Ueda , Hiroto Hara , Tsugumine Shu , Mamoru Hasegawa
IPC分类号： C12P21/00 , C12N5/07
CPC分类号： C12N15/86 , A61K2039/5256 , A61K2039/53 , C07K16/00 , C07K16/1063 , C12N2760/18843
摘要： [Problems to be Solved] The present invention provides methods for producing antibodies and antibody-producing cells.[Means for Solving the Problems] The present invention provides methods for producing antibodies or antibody-producing cells, such methods including the steps of inoculating non-human animals with minus-strand RNA viral vectors carrying nucleic acids which encode foreign polypeptides to be used as antigens, nucleic acids producing the viral vectors, cells into which the vectors or the nucleic acids producing the vectors have been introduced, or lysates of the cells; and collecting the antibodies or antibody-producing cells from the animals. The antibody production can be induced efficiently by the immune activating effect of the minus-strand RNA viral vectors and a high expression of the antigen polypeptides. The antibodies produced by the methods of the present invention can be used in research and development and in the clinical field.
摘要翻译： [待解决的问题]本发明提供了产生抗体和产生抗体的细胞的方法。解决问题的手段本发明提供了产生抗体或抗体产生细胞的方法，包括以下步骤：将携带编码外源多肽的核酸的负链RNA病毒载体接种在非人动物中，以用作为产生病毒载体的核酸，产生载体的载体或核酸的细胞或细胞的裂解物; 并从动物中收集抗体或产生抗体的细胞。可以通过负链RNA病毒载体的免疫激活作用和抗原多肽的高表达来有效诱导抗体产生。通过本发明的方法产生的抗体可用于研究和开发以及临床领域。

4. 发明申请

US20130210150A1 COMPOSITION FOR INDUCING PLURIPOTENT STEM CELL, AND USE THEREOF 有权
标题翻译：用于诱导大脑干细胞的组合物及其用途
公开(公告)号：US20130210150A1
公开(公告)日：2013-08-15
申请号：US13819235
申请日：2011-08-30
申请人： Hiroshi Ban , Yasuji Ueda , Noemi Fusaki , Koichi Saeki , Mamoru Hasegawa
发明人： Hiroshi Ban , Yasuji Ueda , Noemi Fusaki , Koichi Saeki , Mamoru Hasegawa
IPC分类号： C12N15/86
CPC分类号： C12N15/86 , C12N5/0696 , C12N15/63 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2506/13 , C12N2510/00 , C12N2760/18843
摘要： The present invention provides Sendai virus vectors in which genes that encode reprograming factors for inducing pluripotent stem cells are incorporated in a specific order, compositions comprising these vectors for gene delivery to be used in the induction of pluripotent stem cells, and uses thereof. Incorporation of the KLF gene, OCT gene, and SOX gene in a specific order into a single Sendai virus vector successfully and significantly increased the efficiency of pluripotent stem cell induction. Loading multiple reprogramming factors into a single vector can further increase the induction efficiency of pluripotent stem cells while reducing the number of necessary vectors.
摘要翻译：本发明提供仙台病毒载体，其中编码用于诱导多能干细胞的重编程因子的基因以特定顺序并入，包含用于诱导多能干细胞的基因递送的载体的组合物及其用途。将KLF基因，OCT基因和SOX基因以特定顺序并入单一仙台病毒载体中，并显着提高多能干细胞诱导的效率。将多个重编程因子加载到单个载体中可以进一步增加多能干细胞的诱导效率，同时减少必需载体的数量。

5. 发明授权

US07226786B2 Envelope gene-deficient Paramyxovirus vector 有权
标题翻译：包膜基因缺陷型副粘病毒载体
公开(公告)号：US07226786B2
公开(公告)日：2007-06-05
申请号：US10316535
申请日：2002-12-10
申请人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Fumino Tokito , Takahiro Hirata , Tsuyoshi Tokusumi , Makoto Inoue , Yumiko Tokusumi
发明人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Fumino Tokito , Takahiro Hirata , Tsuyoshi Tokusumi , Makoto Inoue , Yumiko Tokusumi
IPC分类号： C12N15/00 , C12N15/86
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , C12N7/00 , C12N2760/18823 , C12N2760/18843 , C12N2760/18845 , C12N2760/18861 , C12N2800/30 , C12N2810/6081
摘要： F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.
摘要翻译：通过使用F基因缺陷的仙台病毒基因组cDNA成功地回收了F基因缺陷型病毒粒子。此外，通过使用F表达细胞作为辅助细胞，成功构建了F基因缺陷型感染性病毒颗粒。此外，通过使用F基因和HN基因缺陷的病毒基因组cDNA，成功地回收了F基因和HN基因缺陷型病毒粒子。此外，通过使用F-和HN表达细胞作为辅助细胞，F基因和HN基因缺陷型感染性病毒颗粒成功产生。通过使用F表达细胞作为辅助细胞构建F基因和HN基因并具有F蛋白的病毒。此外，使用表达M蛋白的辅助细胞产生M基因缺陷型感染性病毒颗粒。从感染M基因缺陷病毒的细胞中，病毒样颗粒的释放被抑制。此外，通过使用表达VSV-G的细胞成功地构建了VSV-G伪型病毒。用于构建这些缺陷病毒的技术有助于可用于基因治疗的副粘病毒科的载体的发育。

6. 发明申请

US20070009949A1 Paramyxovirus-derived RNP 审中-公开
标题翻译：副粘病毒衍生的RNP
公开(公告)号：US20070009949A1
公开(公告)日：2007-01-11
申请号：US11515341
申请日：2006-09-01
申请人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Takahiro Hirata , Makoto Inoue , Yumiko Tokusumi
发明人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Takahiro Hirata , Makoto Inoue , Yumiko Tokusumi
IPC分类号： C12Q1/68 , C12N7/00 , C12N5/00 , C12N7/01 , C12N5/02
CPC分类号： C07K14/005 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , C12N7/00 , C12N15/86 , C12N2710/24143 , C12N2760/18811 , C12N2760/18822 , C12N2760/18823 , C12N2760/18843 , C12N2760/18845 , C12N2760/18861 , C12N2760/20222 , C12N2800/30 , C12N2810/6081
摘要： A functional RNP containing negative-strand single-stranded RNA derived from Sendai virus, which has been modified so as not to express at least one envelope protein, has been successfully prepared. An RNP comprising a foreign gene is prepared and inserted into a cell with the use of a cationic liposome, thereby successfully expressing the foreign gene.
摘要翻译：已经成功地制备了已经被修饰以便不表达至少一种包膜蛋白的来源于仙台病毒的负链单链RNA的功能性RNP。制备包含外来基因的RNP，并使用阳离子脂质体将其插入细胞中，从而成功地表达外源基因。

7. 发明授权

US09090909B2 Composition for inducing pluripotent stem cell, and use thereof 有权
标题翻译：用于诱导多能干细胞的组合物及其用途
公开(公告)号：US09090909B2
公开(公告)日：2015-07-28
申请号：US13819235
申请日：2011-08-30
申请人： Hiroshi Ban , Yasuji Ueda , Noemi Fusaki , Koichi Saeki , Mamoru Hasegawa
发明人： Hiroshi Ban , Yasuji Ueda , Noemi Fusaki , Koichi Saeki , Mamoru Hasegawa
IPC分类号： C12N5/00 , C12N5/02 , C12N15/86 , C12N5/074 , C12N15/63
CPC分类号： C12N15/86 , C12N5/0696 , C12N15/63 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2506/13 , C12N2510/00 , C12N2760/18843
摘要： The present invention provides Sendai virus vectors in which genes that encode reprograming factors for inducing pluripotent stem cells are incorporated in a specific order, compositions comprising these vectors for gene delivery to be used in the induction of pluripotent stem cells, and uses thereof. Incorporation of the KLF gene, OCT gene, and SOX gene in a specific order into a single Sendai virus vector successfully and significantly increased the efficiency of pluripotent stem cell induction. Loading multiple reprogramming factors into a single vector can further increase the induction efficiency of pluripotent stem cells while reducing the number of necessary vectors.
摘要翻译：本发明提供仙台病毒载体，其中编码用于诱导多能干细胞的重编程因子的基因以特定顺序并入，包含用于诱导多能干细胞的基因递送的载体的组合物及其用途。将KLF基因，OCT基因和SOX基因以特定顺序并入单一仙台病毒载体中，并显着提高多能干细胞诱导的效率。将多个重编程因子加载到单个载体中可以进一步增加多能干细胞的诱导效率，同时减少必需载体的数量。

8. 发明授权

US08741639B2 Method for producing dendritic cells 有权
标题翻译：树突状细胞的制备方法
公开(公告)号：US08741639B2
公开(公告)日：2014-06-03
申请号：US13127753
申请日：2009-11-13
申请人： Yoshikazu Yonemitsu , Yasuji Ueda , Yui Harada
发明人： Yoshikazu Yonemitsu , Yasuji Ueda , Yui Harada
IPC分类号： A61K39/00 , C12N5/0784
CPC分类号： C12N5/0639 , A61K39/00 , C12N2501/125 , C12N2501/145 , C12N2501/22 , C12N2501/23 , C12N2501/26
摘要： An objective of the present invention is to provide methods for producing dendritic cells (DCs), which comprise the step of culturing DC precursor cells in the presence of a plurality of cytokines, produced dendritic cells, and uses thereof.The present inventors discovered that dendritic cells with a high IL-12 productivity can be obtained by culturing DC precursor cells in the presence of a plurality of cytokines, followed by about one week of culture in the presence of GM-CSF and IL-4. The present invention enables preparation of a large amount of DCs with a high IL-12 productivity from a small number of DC precursor cells, and therefore makes it easier to increase the number of DCs for administration in DC-based anti-tumor immune therapy, treatment of infections, etc. Thus, the effect of DC vaccines is expected to be enhanced.
摘要翻译：本发明的目的是提供用于制备树突状细胞（DC）的方法，其包括在多种细胞因子，产生的树突状细胞的存在下培养DC前体细胞的步骤及其应用。本发明人发现通过在多种细胞因子的存在下培养DC前体细胞，然后在GM-CSF和IL-4存在下培养约1周，可获得具有高IL-12生产能力的树突状细胞。本发明能够从少量的DC前体细胞制备大量具有高IL-12生产能力的DC，因此可以更容易地增加DC-抗肿瘤免疫治疗中用于给药的DC数量，治疗感染等。因此，预期DC疫苗的效果将得到提高。

9. 发明申请

US20050221292A1 Vectors with modified protease-dependent tropism 有权
标题翻译：具有修饰的蛋白酶依赖性向量的载体
公开(公告)号：US20050221292A1
公开(公告)日：2005-10-06
申请号：US10513094
申请日：2003-04-30
申请人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
发明人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
IPC分类号： A61K38/00 , A61K48/00 , A61P35/00 , C07K14/115 , C12N15/45 , C12N15/86 , C12Q1/70 , C12Q1/68
CPC分类号： C07K14/005 , A61K38/00 , A61K48/00 , C07K2319/50 , C12N15/86 , C12N2760/18022 , C12N2760/18822 , C12N2760/18843 , C12N2800/30
摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.
摘要翻译：本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。

10. 发明申请

US20120040458A1 METHOD FOR PRODUCING DENDRITIC CELLS 有权
标题翻译：生产细胞的方法
公开(公告)号：US20120040458A1
公开(公告)日：2012-02-16
申请号：US13127753
申请日：2009-11-13
申请人： Yoshikazu Yonemitsu , Yasuji Ueda , Yui Harada
发明人： Yoshikazu Yonemitsu , Yasuji Ueda , Yui Harada
IPC分类号： C12N5/0784
CPC分类号： C12N5/0639 , A61K39/00 , C12N2501/125 , C12N2501/145 , C12N2501/22 , C12N2501/23 , C12N2501/26
摘要： An objective of the present invention is to provide methods for producing dendritic cells (DCs), which comprise the step of culturing DC precursor cells in the presence of a plurality of cytokines, produced dendritic cells, and uses thereof.The present inventors discovered that dendritic cells with a high IL-12 productivity can be obtained by culturing DC precursor cells in the presence of a plurality of cytokines, followed by about one week of culture in the presence of GM-CSF and IL-4. The present invention enables preparation of a large amount of DCs with a high IL-12 productivity from a small number of DC precursor cells, and therefore makes it easier to increase the number of DCs for administration in DC-based anti-tumor immune therapy, treatment of infections, etc. Thus, the effect of DC vaccines is expected to be enhanced.
摘要翻译：本发明的目的是提供用于制备树突状细胞（DC）的方法，其包括在多种细胞因子，产生的树突状细胞的存在下培养DC前体细胞的步骤及其应用。本发明人发现通过在多种细胞因子的存在下培养DC前体细胞，然后在GM-CSF和IL-4存在下培养约1周，可获得具有高IL-12生产能力的树突状细胞。本发明能够从少量的DC前体细胞制备大量具有高IL-12生产能力的DC，因此可以更容易地增加DC-抗肿瘤免疫治疗中用于给药的DC数量，治疗感染等。因此，预期DC疫苗的效果将得到提高。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式