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1. 发明授权

US07759119B2 Systems and methods for efficient collection of single cells and colonies of cells and fast generation of stable transfectants 有权
标题翻译：有效收集单个细胞和细胞菌落的系统和方法，并快速产生稳定的转染子
公开(公告)号：US07759119B2
公开(公告)日：2010-07-20
申请号：US11733053
申请日：2007-04-09
申请人： Nancy Allbritton , Christopher E. Sims , Yuli Wang , Mark Bachman , Guann-Pyng Li , Eric Stanbridge
发明人： Nancy Allbritton , Christopher E. Sims , Yuli Wang , Mark Bachman , Guann-Pyng Li , Eric Stanbridge
IPC分类号： C12N15/00 , C12N5/02 , C12N5/00 , C12N15/01 , C12N1/02
CPC分类号： G01N1/286 , B01L3/5085 , B01L2200/0668 , B01L2300/0829 , C12M23/12 , C12M47/04 , G01N2001/2886
摘要： A plate manufactured to enable samples of cells, micro-organisms, proteins, DNA, biomolecules and other biological media to be positioned at specific locations or sites on the plate for the purpose of performing addressable analyses on the samples. Preferably, some or all of the sites are built from a removable material or as pallets so that a subset of the samples of interest can be readily isolated from the plate for further processing or analysis. The plate can contain structures or chemical treatments that enhance or promote the attachment and/or function of the samples, and that promote or assist in their analyses. Use of the plate advantageously enables the selection and sorting of cells based on dynamic phenomena and the rapid establishment of stable tranfectants.
摘要翻译：制造的板可使细胞，微生物，蛋白质，DNA，生物分子和其他生物培养基的样品定位在板上的特定位置或位点，以便对样品进行可寻址分析。优选地，一些或所有的部位由可移除的材料或托盘构建，使得感兴趣的样品的子集可以容易地与板分离以用于进一步的处理或分析。该板可以包含增强或促进样品的附着和/或功能的结构或化学处理，并且促进或辅助其分析。板的使用有利于基于动态现象和快速建立稳定的转染剂来选择和分选细胞。

2. 发明授权

US08748085B2 Use of photosensitized Epon epoxy resin 1002F for MEMS and bioMEMS applications 有权
标题翻译：光敏Epon环氧树脂1002F用于MEMS和bioMEMS应用
公开(公告)号：US08748085B2
公开(公告)日：2014-06-10
申请号：US12670349
申请日：2008-07-25
申请人： Jeng-Hao Pai , Yuli Wang , Christopher E. Sims , Mark Bachman , Nancy Allbritton , Guann-Pyng Li
发明人： Jeng-Hao Pai , Yuli Wang , Christopher E. Sims , Mark Bachman , Nancy Allbritton , Guann-Pyng Li
IPC分类号： G03F7/26
CPC分类号： G03F7/40 , B01L3/502707 , G03F1/50 , G03F7/0037 , G03F7/038 , G03F7/095
摘要： Systems and methods directed to the use 1002F to build microdevices and biomedical devices. Through the addition of a photosensitizing agent, Epon epoxy resin 1002F can be linked in the presence of UV light, making it useful as a photoresist or as a micropatternable structural material. One embodiment comprises combining 1002F monomer resin with a solvent and a photoinitiator, placing the monomer solution on a surface, exposing the monomer solution to UV light through a mask to initiate linking, and stripping the unlinked polymer away. In another embodiment, 3-D structures are built using two or more layers of sensitized monomer films, each having different sensitivity to light, and the use of a mask containing opaque and semi-opaque regions.
摘要翻译：针对使用1002F构建微型设备和生物医学设备的系统和方法。通过添加光敏剂，Epon环氧树脂1002F可以在UV光的存在下连接，使其可用作光致抗蚀剂或作为微图案化的结构材料。一个实施方案包括将1002F单体树脂与溶剂和光引发剂组合，将单体溶液置于表面上，通过掩模将单体溶液暴露于UV光以引发连接，并将未连接的聚合物剥离。在另一个实施方案中，使用两层或更多层敏化单体膜来构建3-D结构，每层具有不同的光敏感性，以及使用含有不透明区域和半不透明区域的掩模。

3. 发明授权

US08383378B2 Micro-bubble plate for patterning biological and non-biological materials 有权
标题翻译：用于生物和非生物材料图案的微泡板
公开(公告)号：US08383378B2
公开(公告)日：2013-02-26
申请号：US11539695
申请日：2006-10-09
申请人： Yuli Wang , Mark Bachman , Christopher E. Sims , Guann-Pyng Li , Nancy Allbritton
发明人： Yuli Wang , Mark Bachman , Christopher E. Sims , Guann-Pyng Li , Nancy Allbritton
IPC分类号： C12N11/02 , C12Q1/68 , C12M1/34
CPC分类号： B01L3/5085 , B01L3/5088 , B01L2200/0642 , B01L2200/12 , B01L2300/0819 , C12M25/06 , C12M29/20
摘要： Systems and methods are provided for patterning biological and non-biological material at specific sites on a plate, as well as growing three dimensional structures. Preferred embodiments comprise a plate with regions that will trap gas, usually in the form of bubbles, when the plate is submerged in liquid. Other embodiment of the present invention include a method for placing materials on the plate at pre-determined locations through the use of trapped gas to prevent materials from collecting at unwanted regions. The plate has great utility for plating cells and tissues at specific sites, such as on an array. The disclosed method can also be used to coat the surface of a plate with coatings at specific locations for patterned coating applications and to build up materials to produce three dimensional structures, including micro-mechanical structures—where the structures may be formed from living or non-living material, tissue or non-tissue, organic or inorganic, and the like.
摘要翻译：提供了系统和方法，用于在板上的特定位置图案化生物和非生物材料，以及生长三维结构。优选实施例包括具有区域的板，当板被浸没在液体中时，其具有通常以气泡形式捕获气体的区域。本发明的其它实施方案包括通过使用捕获的气体将材料放置在预定位置处的材料的方法，以防止材料在不需要的区域收集。该板对于在特定位置（例如阵列）上的电镀细胞和组织具有很大的用途。所公开的方法还可以用于在具有特定位置的涂层涂覆板的表面以用于图案化涂布应用，并且建立材料以产生三维结构，包括微机械结构，其中结构可以由活的或非 - 生物材料，组织或非组织，有机或无机物等。

4. 发明申请

US20110223545A1 USE OF PHOTOSENSITIZED EPON EPOXY RESIN 1002F FOR MEMS AND BIOMEMS APPLICATIONS 有权
标题翻译：光敏环氧树脂1002F用于MEMS和生物物质的应用
公开(公告)号：US20110223545A1
公开(公告)日：2011-09-15
申请号：US12670349
申请日：2008-07-25
申请人： Jeng-Hao Pai , Yuli Wang , Christopher E. Sims , Mark Bachman
发明人： Jeng-Hao Pai , Yuli Wang , Christopher E. Sims , Mark Bachman
IPC分类号： G03F7/20
CPC分类号： G03F7/40 , B01L3/502707 , G03F1/50 , G03F7/0037 , G03F7/038 , G03F7/095
摘要： Systems and methods directed to the use 1002F to build microdevices and biomedical devices. Through the addition of a photosensitizing agent, Epon epoxy resin 1002F can be linked in the presence of UV light, making it useful as a photoresist or as a micropatternable structural material. One embodiment comprises combining 1002F monomer resin with a solvent and a photoinitiator, placing the monomer solution on a surface, exposing the monomer solution to UV light through a mask to initiate linking, and stripping the unlinked polymer away. In another embodiment, 3-D structures are built using two or more layers of sensitized monomer films, each having different sensitivity to light, and the use of a mask containing opaque and semi-opaque regions.
摘要翻译：针对使用1002F构建微型设备和生物医学设备的系统和方法。通过添加光敏剂，Epon环氧树脂1002F可以在UV光的存在下连接，使其可用作光致抗蚀剂或作为微图案化的结构材料。一个实施方案包括将1002F单体树脂与溶剂和光引发剂组合，将单体溶液置于表面上，通过掩模将单体溶液暴露于UV光以引发连接，并将未连接的聚合物剥离。在另一个实施方案中，使用两层或更多层敏化单体膜来构建3-D结构，每层具有不同的光敏感性，以及使用含有不透明区域和半不透明区域的掩模。

5. 发明授权

US06335201B1 Method and apparatus for detecting enzymatic activity using molecules that change electrophoretic mobility 失效
公开(公告)号：US06335201B1
公开(公告)日：2002-01-01
申请号：US09358504
申请日：1999-07-21
申请人： Nancy L. Allbritton , Christopher E. Sims , Michael W. Berns , Gavin D. Meredith , Tatiana B. Krasieva , Bruce J. Tromberg
发明人： Nancy L. Allbritton , Christopher E. Sims , Michael W. Berns , Gavin D. Meredith , Tatiana B. Krasieva , Bruce J. Tromberg
IPC分类号： G01N3348
CPC分类号： C12M41/46 , C12M35/02 , C12M47/06 , C12Q1/00 , C12Q1/485 , G01N27/44721 , G01N27/44743 , G01N33/50 , G01N33/5017
摘要： The activity of intracellular chemical reactions of molecules is measured by the use of fluorescently labeled substrate molecules that undergo a change in electrophoretic mobility upon chemical reaction such as that catalyzed by an enzyme. Specificity is achieved by using labeled substrate molecules that can be acted upon only by specific enzymes. Thus the activity of a specific enzyme or class of enzymes can be determined. Measurements are made with the intracellular presence of such substrate molecules, at some time of interest, typically after exposure of the cell to a stimulus that activates a particular enzymatic pathway. To ensure accuracy, measurements must be made in a timely manner so as to minimize chemical reactions occurring subsequent to the time of interest. Fast controllable laser lysis is used to obtain the contents of a single cell into which reporter substrate molecules have been introduced. The cell contents are then subjected to capillary electrophoresis and enzymatic activity is determined by comparing amounts of substrate molecules to amounts of enzymatically altered substrate molecules which are separated by the electrophoresis and identified by the presence of a fluorescent label.

6. 发明授权

US6156576A Fast controllable laser lysis of cells for analysis 失效
标题翻译：快速可控激光裂解细胞进行分析
公开(公告)号：US6156576A
公开(公告)日：2000-12-05
申请号：US36706
申请日：1998-03-06
申请人： Nancy L. Allbritton , Christopher E. Sims , Michael W. Berns , Gavin D. Meredith , Tatiana B. Krasieva , Bruce J. Tromberg
发明人： Nancy L. Allbritton , Christopher E. Sims , Michael W. Berns , Gavin D. Meredith , Tatiana B. Krasieva , Bruce J. Tromberg
IPC分类号： G01N33/48 , C12M1/33 , C12N13/00 , G01N27/447 , G01N33/483
CPC分类号： G01N27/44743 , C12M47/06 , G01N27/44721
摘要： Fast lysis of a single cell or cellular component thereof is performed by generating a shock wave in a medium in which the cell or cellular component thereof is positioned. The cell or cellular component thereof is either positioned by laser tweezers or cultured as an adhered cell or cellular component thereof to minimize manipulation trauma. The disclosed method completely lyses a single cell or cellular component thereof in a controllable manner in milliseconds or less followed immediately by the loading of the cellular contents into a capillary for analyte separation and detection. The cell or cellular component thereof is adjacent the inlet of an electrophoretic column through which a gravity siphon flow of the medium is maintained. The lysed contents of the cell or cellular component thereof enter the electrophoretic column in less than 33 msec, are separated and analyzed by laser induced fluorescence. The method takes advantage of the shock wave produced by a highly focused laser pulse which is created in a medium adjacent to the cell or cellular component thereof. In the illustrated embodiment the laser pulse is focused in the glass substrate at or near a glass-to-buffer interface of a cell chamber in which the cell or cellular component thereof to be lysed has been cultured.
摘要翻译：单细胞或其细胞成分的快速裂解是通过在细胞或细胞成分定位的培养基中产生冲击波来进行的。其细胞或细胞组分由激光镊子定位或作为粘附细胞或其细胞组分培养，以最小化操作创伤。所公开的方法以几毫秒或更短的可控方式完全溶解单细胞或细胞组分，随后将细胞内容物加载到用于分析物分离和检测的毛细管中。其电池或细胞组分邻近电泳柱的入口，通过该入口保持介质的重力虹吸流。细胞或细胞成分的裂解内容物在小于33毫秒内进入电泳柱，通过激光诱导荧光分离和分析。该方法利用了在与其细胞或细胞组分相邻的介质中产生的高度聚焦的激光脉冲产生的冲击波。在所示实施例中，激光脉冲聚焦在玻璃基板中或其附近，其中待裂解细胞或其细胞组分的细胞室的玻璃 - 缓冲液界面已被培养。

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