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    • 3. 发明授权
    • Protein exhibiting activity of pyrethrin biosynthetic enzyme, gene encoding the protein, and vector bearing the gene
    • 蛋白质表现出除虫菊酯生物合成酶的活性,编码蛋白质的基因和携带该基因的载体
    • US08129180B2
    • 2012-03-06
    • US12457193
    • 2009-06-03
    • Kazuhiko MatsudaYukio Kikuta
    • Kazuhiko MatsudaYukio Kikuta
    • C12N15/63C12N15/54C12N9/10
    • C12N9/14C07K14/415C12N9/1029
    • The present invention relates to an enzyme determining amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin; and the enzyme is a gene encoding a protein of the following (i) or (ii): (i) a protein consisting of an amino acid sequence shown in Sequence No. 1; or (ii) a protein consisting of an amino acid sequence including one or more of a substitution, deletion, insertion, and/or addition of amino acid in the amino acid sequence shown in Sequence No. 1, in which the protein exhibits activity of pyrethrin biosynthetic enzyme.
    • 本发明涉及涉及除虫菊酯生物合成的酶的酶测定氨基酸序列及其基因的碱基序列; 构建带有基因和转化体的载体; 并通过将这种创造性技术应用于具有更快增长的植物体,从而产生除虫菊酯的植物体中提取,目的在于提供一种有效生产除虫菊酯的方法; 该酶是编码以下(i)或(ii)的蛋白质的基因:(i)由序列号1所示的氨基酸序列组成的蛋白质; 或者(ii)由序列号1所示的氨基酸序列中氨基酸序列中包含氨基酸的取代,缺失,插入和/或添加中的一个或多个氨基酸序列组成的蛋白质,其中蛋白质表现出 除虫菊酯生物合成酶。
    • 4. 发明申请
    • Protein exhibiting activity of pyrethrin biosynthetic enzyme, gene encoding the protein, and vector bearing the gene
    • 蛋白质表现出除虫菊酯生物合成酶的活性,编码蛋白质的基因和携带该基因的载体
    • US20100062510A1
    • 2010-03-11
    • US12457193
    • 2009-06-03
    • Kazuhiko MatsudaYukio Kikuta
    • Kazuhiko MatsudaYukio Kikuta
    • C12N9/00C07H21/00C12N15/63
    • C12N9/14C07K14/415C12N9/1029
    • The present invention relates to an enzyme determining amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin; and the enzyme is a gene encoding a protein of the following (i) or (ii): (i) a protein consisting of an amino acid sequence shown in Sequence No. 1; or (ii) a protein consisting of an amino acid sequence including one or more of a substitution, deletion, insertion, and/or addition of amino acid in the amino acid sequence shown in Sequence No. 1, in which the protein exhibits activity of pyrethrin biosynthetic enzyme.
    • 本发明涉及涉及除虫菊酯生物合成的酶的酶测定氨基酸序列及其基因的碱基序列; 构建带有基因和转化体的载体; 并通过将这种创造性技术应用于具有更快增长的植物体,从而产生除虫菊酯的植物体中提取,目的在于提供一种有效生产除虫菊酯的方法; 该酶是编码以下(i)或(ii)的蛋白质的基因:(i)由序列号1所示的氨基酸序列组成的蛋白质; 或者(ii)由序列号1所示的氨基酸序列中氨基酸序列中包含氨基酸的取代,缺失,插入和/或添加中的一个或多个氨基酸序列组成的蛋白质,其中蛋白质表现出 除虫菊酯生物合成酶。
    • 6. 发明授权
    • Protein exhibiting activity of pyrethrin biosynthetic enzyme, gene encoding the protein, and vector bearing the gene
    • 蛋白质表现出除虫菊酯生物合成酶的活性,编码蛋白质的基因和携带该基因的载体
    • US08759072B2
    • 2014-06-24
    • US13137327
    • 2011-08-05
    • Kazuhiko MatsudaYukio Kikuta
    • Kazuhiko MatsudaYukio Kikuta
    • C12N15/54C12N9/10
    • C07K14/415C12N9/1029C12N9/14C12N15/8243
    • The present invention relates to an enzyme determining amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin; and the enzyme is a gene encoding a protein of the following (i) or (ii) or (iii): (i) a protein consisting of an amino acid sequence shown in Sequence No. 1; or (ii) a protein consisting of an amino acid sequence shown in Sequence No. 5, or a protein consisting of an amino acid sequence shown in Sequence No. 6, or a protein consisting of an amino acid sequence shown in Sequence No. 7, or a protein consisting of an amino acid sequence shown in Sequence No. 8, (iii) a protein consisting of an amino acid sequence including one or more of a substitution, deletion, insertion, and/or addition of amino acid in the amino acid sequence shown in Sequence No. 1, in which the protein exhibits activity of pyrethrin biosynthetic enzyme.
    • 本发明涉及涉及除虫菊酯生物合成的酶的酶测定氨基酸序列及其基因的碱基序列; 构建带有基因和转化体的载体; 并通过将这种创造性技术应用于具有更快增长的植物体,从而产生除虫菊酯的植物体中提取,目的在于提供一种有效生产除虫菊酯的方法; 该酶是编码以下(i)或(ii)或(iii)的蛋白质的基因:(i)由序列号1所示的氨基酸序列组成的蛋白质; 或者(ii)由序列号5所示的氨基酸序列组成的蛋白质或由序列号6所示的氨基酸序列组成的蛋白质,或由序列号7所示的氨基酸序列组成的蛋白质 或由序列号8所示的氨基酸序列组成的蛋白质,(iii)由氨基酸序列构成的蛋白质,该氨基酸序列包含氨基酸中氨基酸的取代,缺失,插入和/或添加中的一个或多个 序列号1所示的酸序列,其中蛋白质显示除虫菊酯生物合成酶的活性。
    • 8. 发明申请
    • Protein exhibiting activity of pyrethrin biosynthetic enzyme, gene encoding the protein, and vector bearing the gene
    • 蛋白质表现出除虫菊酯生物合成酶的活性,编码蛋白质的基因和携带该基因的载体
    • US20120040434A1
    • 2012-02-16
    • US13137327
    • 2011-08-05
    • Kazuhiko MatsudaYukio Kikuta
    • Kazuhiko MatsudaYukio Kikuta
    • C12N9/16C12N15/63C12N15/55
    • C07K14/415C12N9/1029C12N9/14C12N15/8243
    • The present invention relates to an enzyme determining amino acid sequences of an enzyme involved in pyrethrin biosynthesis and a base sequence of the gene thereof; constructing vectors bearing the gene and transformants; and extractable from plant bodies producing pyrethrin by applying such creative techniques to plant bodies with faster growth aiming to provide a method to efficiently produce pyrethrin; and the enzyme is a gene encoding a protein of the following (i) or (ii) or (iii): (i) a protein consisting of an amino acid sequence shown in Sequence No. 1; or (ii) a protein consisting of an amino acid sequence shown in Sequence No. 5, or a protein consisting of an amino acid sequence shown in Sequence No. 6, or a protein consisting of an amino acid sequence shown in Sequence No. 7, or a protein consisting of an amino acid sequence shown in Sequence No. 8, (iii) a protein consisting of an amino acid sequence including one or more of a substitution, deletion, insertion, and/or addition of amino acid in the amino acid sequence shown in Sequence No. 1, in which the protein exhibits activity of pyrethrin biosynthetic enzyme.
    • 本发明涉及涉及除虫菊酯生物合成的酶的酶测定氨基酸序列及其基因的碱基序列; 构建带有基因和转化体的载体; 并通过将这种创造性技术应用于具有更快增长的植物体,从而产生除虫菊酯的植物体中提取,目的在于提供一种有效生产除虫菊酯的方法; 该酶是编码以下(i)或(ii)或(iii)的蛋白质的基因:(i)由序列号1所示的氨基酸序列组成的蛋白质; 或者(ii)由序列号5所示的氨基酸序列组成的蛋白质或由序列号6所示的氨基酸序列组成的蛋白质,或由序列号7所示的氨基酸序列组成的蛋白质 或由序列号8所示的氨基酸序列组成的蛋白质,(iii)由氨基酸序列构成的蛋白质,该氨基酸序列包含氨基酸中氨基酸的取代,缺失,插入和/或添加中的一个或多个 序列号1所示的酸序列,其中蛋白质显示除虫菊酯生物合成酶的活性。