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1. 发明申请

US20080254019A1 Therapeutic Agents and Therapeutic Methods For Treating Injured Tissue 有权
标题翻译：治疗药物和治疗方法治疗受伤组织
公开(公告)号：US20080254019A1
公开(公告)日：2008-10-16
申请号：US10594595
申请日：2005-03-31
申请人： Yukio Kato , Masahiro Nishimura , Yoshie Ozaki , Koichiro Tsuji
发明人： Yukio Kato , Masahiro Nishimura , Yoshie Ozaki , Koichiro Tsuji
IPC分类号： A61K38/48 , C07K14/00 , C12N9/74 , A61P17/02 , A61K38/16
CPC分类号： A61K31/728 , A61K35/28 , C12N5/0663 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/905 , C12N2501/998 , A61K2300/00
摘要： This invention provides therapeutic agents, transplants and therapeutic methods that can enhance the regeneration of injured tissue. This invention relates to agents, transplants and therapeutic methods for enhancing the migration and accumulation of mesenchymal stem cells in injured tissues and/or suppressing the diffusion of mesenchymal stem cells from injured tissues.
摘要翻译：本发明提供可以增强损伤组织再生的治疗剂，移植物和治疗方法。本发明涉及用于增强受损组织中间充质干细胞的迁移和积累和/或抑制间充质干细胞从损伤组织扩散的药剂，移植物和治疗方法。

2. 发明授权

US08119397B2 Therapeutic agents and therapeutic methods for treating injured tissue 有权
标题翻译：用于治疗损伤组织的治疗剂和治疗方法
公开(公告)号：US08119397B2
公开(公告)日：2012-02-21
申请号：US10594595
申请日：2005-03-31
申请人： Yukio Kato , Masahiro Nishimura , Yoshie Ozaki , Koichiro Tsuji
发明人： Yukio Kato , Masahiro Nishimura , Yoshie Ozaki , Koichiro Tsuji
IPC分类号： C12N5/00 , A61K38/16 , C07K1/00
CPC分类号： A61K31/728 , A61K35/28 , C12N5/0663 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/905 , C12N2501/998 , A61K2300/00
摘要： This invention provides therapeutic agents, transplants and therapeutic methods that can enhance the regeneration of injured tissue. This invention relates to agents, transplants and therapeutic methods for enhancing the migration and accumulation of mesenchymal stem cells in injured tissues and/or suppressing the diffusion of mesenchymal stem cells from injured tissues.
摘要翻译：本发明提供可以增强损伤组织再生的治疗剂，移植物和治疗方法。本发明涉及用于增强受损组织中间充质干细胞的迁移和积累和/或抑制间充质干细胞从损伤组织扩散的药剂，移植物和治疗方法。

3. 发明授权

US09234897B2 Method for distinguishing mesenchymal stem cell using molecular marker and use thereof 有权
标题翻译：使用分子标记鉴别间充质干细胞的方法及其用途
公开(公告)号：US09234897B2
公开(公告)日：2016-01-12
申请号：US11887362
申请日：2006-03-30
申请人： Yukio Kato , Takeshi Kawamoto , Koichiro Tsuji , Akira Igarashi , Masakazu Shimizu
发明人： Yukio Kato , Takeshi Kawamoto , Koichiro Tsuji , Akira Igarashi , Masakazu Shimizu
IPC分类号： C12N5/00 , G01N33/68 , C12Q1/68 , G01N33/569
CPC分类号： G01N33/6845 , C12Q1/6881 , C12Q2600/158 , G01N33/56966
摘要： Disclosed is a method for distinguishing a mesenchymal stem cell comprising, using at least one gene selected from the genes having the nucleotide sequences indicated by the accession numbers shown in Table 1 as a distinguish marker, detecting the difference in expression of the distinguish marker between a mesenchymal stem cell and a connective tissue cell to distinguish the mesenchymal stem cell from the connective tissue cell. This method enables to distinguish an undifferentiated mesenchymal stem cell from other connective tissue cell such as fibroblasts, osteoblasts, chondrocytes and adipose cells with good accuracy. A mesenchymal stem cell given by this method or a composition comprising the mesenchymal stem sell can be used as a therapeutic for use in the regenerative medicine.
摘要翻译：本发明公开了一种区分间充质干细胞的方法，其特征在于，使用选自具有表1所示的登录号的核苷酸序列的基因的至少一种基因作为区别标记，检测所述区别标记的表达差异间充质干细胞和结缔组织细胞，以区分间充质干细胞与结缔组织细胞。该方法能够以良好的精度将未分化的间充质干细胞与其他结缔组织细胞如成纤维细胞，成骨细胞，软骨细胞和脂肪细胞区分开。通过该方法给予的间充质干细胞或包含间充质茎的组合物可用作再生医学中的治疗剂。

4. 发明申请

US20110212523A1 DIFFERENTIATION-INDUCING CULTURE MEDIUM ADDITIVE AND USE THEREOF 审中-公开
标题翻译：差异诱导培养基添加剂及其用途
公开(公告)号：US20110212523A1
公开(公告)日：2011-09-01
申请号：US13127774
申请日：2009-10-22
申请人： Yukio Kato , Jin Chang Shao , Koichiro Tsuji
发明人： Yukio Kato , Jin Chang Shao , Koichiro Tsuji
IPC分类号： C12N5/077
CPC分类号： C12N5/0654 , C12N2500/25 , C12N2500/42 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/39 , C12N2501/40 , C12N2506/1353
摘要： Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.
摘要翻译：本发明提供一种分化诱导培养基添加剂，其用于诱导选自干细胞，牙髓细胞，牙周韧带细胞，胎盘，羊膜和成纤维细胞中的至少一种类型的细胞的骨分化。无血清条件，以及使用分化诱导培养基添加剂。用于在无血清条件下诱导干细胞分化的本发明的分化诱导培养基添加剂至少含有至少一种选自EGF，FGF和PDGF的生长因子; 地塞米松和甘油磷酸酯。本发明的分化诱导培养基添加剂不需要抗坏血酸2-磷酸酯和ITS，它们通常对骨分化至关重要。此外，通过添加磷脂可以促进骨分化。

5. 发明申请

US20090232773A1 Method for Distinguishing Mesenchymal Stem Cell Using Molecular Marker and Use Thereof 有权
标题翻译：使用分子标记鉴别间充质干细胞的方法及其用途
公开(公告)号：US20090232773A1
公开(公告)日：2009-09-17
申请号：US11887362
申请日：2006-03-30
申请人： Yukio Kato , Takeshi Kawamoto , Koichiro Tsuji , Akira Igarashi , Masakazu Shimizu
发明人： Yukio Kato , Takeshi Kawamoto , Koichiro Tsuji , Akira Igarashi , Masakazu Shimizu
IPC分类号： A61K35/12 , C12Q1/68 , C12N5/00 , C40B30/04 , C40B40/02 , A61K31/7088 , C07K2/00 , C07K16/00 , C07H21/04
CPC分类号： G01N33/6845 , C12Q1/6881 , C12Q2600/158 , G01N33/56966
摘要： Disclosed is a method for distinguishing a mesenchymal stem cell comprising, using at least one gene selected from the genes having the nucleotide sequences indicated by the accession numbers shown in Table 1 as a distinguish marker, detecting the difference in expression of the distinguish marker between a mesenchymal stem cell and a connective tissue cell to distinguish the mesenchymal stem cell from the connective tissue cell. This method enables to distinguish an undifferentiated mesenchymal stem cell from other connective tissue cell such as fibroblasts, osteoblasts, chondrocytes and adipose cells with good accuracy. A mesenchymal stem cell given by this method or a composition comprising the mesenchymal stem sell can be used as a therapeutic for use in the regenerative medicine.
摘要翻译：本发明公开了一种区分间充质干细胞的方法，其特征在于，使用选自具有表1所示的登录号的核苷酸序列的基因的至少一种基因作为区别标记，检测所述区别标记的表达差异间充质干细胞和结缔组织细胞，以区分间充质干细胞与结缔组织细胞。该方法能够以良好的精度将未分化的间充质干细胞与其他结缔组织细胞如成纤维细胞，成骨细胞，软骨细胞和脂肪细胞区分开。通过该方法给予的间充质干细胞或包含间充质茎的组合物可用作再生医学中的治疗剂。

6. 发明申请

US20090221076A1 METHOD OF FABRICATING SHEET FOR CARTILAGE TISSUE REGENERATION 审中-公开
标题翻译：制备组织再生的方法
公开(公告)号：US20090221076A1
公开(公告)日：2009-09-03
申请号：US11817437
申请日：2006-02-28
申请人： Yukio Kato , Koichiro Tsuji , Katsuyuki Yamanaka
发明人： Yukio Kato , Koichiro Tsuji , Katsuyuki Yamanaka
IPC分类号： C12N5/02
CPC分类号： A61L27/18 , A61L27/3817 , A61L27/3852 , A61L27/56 , A61L2430/06 , C12N5/0655 , C12N2501/15 , C12N2501/39 , C12N2533/40 , C08L67/04
摘要： An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglycolic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000 G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration.
摘要翻译：本发明的目的是提供一种用于再生软骨组织的片材的制造方法，其使用包含生物吸收性合成高分子如聚乳酸，聚乙醇酸和乳酸共聚物的常规片状多孔体酸和乙醇酸。用于再生软骨组织的片材可以分化软骨细胞或干细胞，而不用像常规方法那样通过加压培养细胞而不培养细胞，并且可以将细胞以高效率积累在支持载体中。用于再生软骨组织的片材通过以下步骤制备：在包含生物吸收性合成高分子的片状多孔体上接种与软骨细胞分化的软骨细胞或干细胞; 将多孔体吸入培养液中; 对多孔体施加100〜1000G的加速度一定时间; 并且在不施加加速度的情况下培养多孔体。

7. 发明授权

US09394521B2 Cell preparation containing mesenchymal stem cells, and method for producing same 有权
标题翻译：含有间充质干细胞的细胞制剂及其制备方法
公开(公告)号：US09394521B2
公开(公告)日：2016-07-19
申请号：US13583150
申请日：2011-03-10
申请人： Toshie Tsuchiya , Koichiro Tsuji , Yukio Kato , Jin Chang Shao , Maiko Hara
发明人： Toshie Tsuchiya , Koichiro Tsuji , Yukio Kato , Jin Chang Shao , Maiko Hara
IPC分类号： C12N5/0775 , C12Q1/06 , A61K35/12
CPC分类号： C12N5/0663 , A61K35/12 , C12N5/0668 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15
摘要： A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).
摘要翻译：通过无血清或低血清培养物产生含有维持免疫抑制能力的间充质干细胞的细胞制剂。一种含有间充质干细胞的细胞制剂的制备方法，包括以下步骤：（A）在含有FGF，PDGF，TGF-βb，HGF，HGF的无血清培养基“A”中增殖间充质干细胞 EGF，至少一种磷脂和至少一种脂肪酸; 和（B）从步骤（A）中因此增殖的间充质干细胞筛选其免疫抑制能力维持或改善的间充质干细胞。

8. 发明授权

US09074176B2 Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof 有权
标题翻译：用于动物细胞无血清培养的培养基添加剂，试剂盒及其用途
公开(公告)号：US09074176B2
公开(公告)日：2015-07-07
申请号：US12160481
申请日：2007-01-11
申请人： Yukio Kato , Jin Chang Shao , Yuki Katsura , Koichiro Tsuji
发明人： Yukio Kato , Jin Chang Shao , Yuki Katsura , Koichiro Tsuji
IPC分类号： C12N5/02 , C12N5/00
CPC分类号： C12N5/0663 , C12N5/0031 , C12N5/0037 , C12N5/0686 , C12N2500/14 , C12N2500/16 , C12N2500/30 , C12N2500/32 , C12N2500/36 , C12N2500/38 , C12N2500/90 , C12N2501/10 , C12N2501/11 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/135 , C12N2501/15 , C12N2501/165 , C12N2501/33 , C12N2501/39 , C12N2501/40
摘要： Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.
摘要翻译：公开了：含有特定生长因子和至少一种磷脂的培养基; 用于制备培养基的组合物; 一套工具和一种方法。可以提供一种技术，其使用无血清或低血清培养基，并且与在含血清培养基中培养获得的促进作用相当，对动物细胞的增殖具有促进作用。

9. 发明申请

US20060166214A1 Marker for detecting mesenchymal stem cell and method of distinguishing mesenchymal stem cell using the marker 审中-公开
标题翻译：用于检测间充质干细胞的标记和使用标记区分间充质干细胞的方法
公开(公告)号：US20060166214A1
公开(公告)日：2006-07-27
申请号：US10548681
申请日：2004-02-27
申请人： Yukio Kato , Koichiro Tsuji , Chika Koike
发明人： Yukio Kato , Koichiro Tsuji , Chika Koike
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12N15/1138 , C07K14/47 , C12Q1/6881 , C12Q2600/158
摘要： The present invention provides a marker for detecting, separating/distinguishing a mesenchymal stem cell and a method for detecting, separating/distinguishing a mesenchymal stem cell by using the marker. A gene shown in the sequence listing is expressed specifically in a mesenchymal stem cell. The present invention comprises use of the gene as a marker for detecting mesenchymal stem cells. In addition, the present invention comprises a probe for detecting the mesenchymal stem cell marker gene and a PCR primer for amplifying the gene in a test cell in detecting the mesenchymal stem cell gene marker, and further comprises a polypeptide marker for detecting mesenchymal stem cells comprising a polypeptide wherein the mesenchymal stem cell marker gene of the present invention is expressed, an antibody for detecting the polypeptide marker which specifically binds to the polypeptide marker, and still further, a method for distinguishing and separating a mesenchymal stem cell by using a probe for detecting the mesenchymal stem cell marker gene, and an antibody which specifically binds to a polypeptide marker.
摘要翻译：本发明提供了用于检测，分离/区分间充质干细胞的标记物和通过使用标记物检测，分离/鉴别间充质干细胞的方法。序列表中所示的基因在间充质干细胞中特异性表达。本发明包括使用该基因作为检测间充质干细胞的标志物。此外，本发明包括用于检测间充质干细胞标记基因的探针和用于在检测间充质干细胞基因标记物中扩增测试细胞中的基因的PCR引物，并且还包含用于检测间充质干细胞的多肽标记，包括其中表达本发明的间充质干细胞标记基因的多肽，用于检测与多肽标记特异性结合的多肽标记物的抗体，还有，通过使用探针来鉴别和分离间充质干细胞的方法检测间充质干细胞标记基因，以及特异性结合多肽标记的抗体。

10. 发明申请

US20120329087A1 CELL PREPARATION CONTAINING MESENCHYMAL STEM CELLS, AND METHOD FOR PRODUCING SAME 有权
标题翻译：含有中间干细胞的细胞制备方法及其生产方法
公开(公告)号：US20120329087A1
公开(公告)日：2012-12-27
申请号：US13583150
申请日：2011-03-10
申请人： Toshie Tsuchiya , Koichiro Tsuji , Yukio Kato , Jin Chang Shao , Maiko Hara
发明人： Toshie Tsuchiya , Koichiro Tsuji , Yukio Kato , Jin Chang Shao , Maiko Hara
IPC分类号： C12N5/0775 , C12Q1/06
CPC分类号： C12N5/0663 , A61K35/12 , C12N5/0668 , C12N2500/90 , C12N2501/11 , C12N2501/115 , C12N2501/12 , C12N2501/135 , C12N2501/15
摘要： A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium “A” containing an FGF, a PDGF, a TGF-β, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).
摘要翻译：通过无血清或低血清培养物产生含有维持免疫抑制能力的间充质干细胞的细胞制剂。一种含有间充质干细胞的细胞制剂的制备方法，包括以下步骤：（A）在含有FGF，PDGF，TGF-β1，HGF，EGF的无血清培养基A中增殖间充质干细胞，至少一种磷脂和至少一种脂肪酸; 和（B）从步骤（A）中因此增殖的间充质干细胞筛选其免疫抑制能力维持或改善的间充质干细胞。

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