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    • 8. 发明授权
    • Method for measuring surface plasmon resonance
    • 表面等离子体共振测量方法
    • US07449343B2
    • 2008-11-11
    • US11218656
    • 2005-09-06
    • Hirohiko Tsuzuki
    • Hirohiko Tsuzuki
    • G01N33/558G01N33/553
    • G01N33/54373Y10S436/805
    • It is an object of the present invention to provide a measurement method which can suppress noise when a specific binding reaction between a physiologically active substance and a test substance is measured using a surface plasmon resonance measurement device. The present invention provides a method for measuring a change in surface plasmon resonance which comprises: using a surface plasmon resonance measurement device comprising a metal film, a light source for generating a light beam, an optical system for allowing the above-described light beam to enter the interface of the metal film so that total reflection conditions can be obtained at the interface thereof and so that various incidence angles can be included, a flow channel system comprising a cell formed on the above-described metal film, and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of the light beam totally reflected at the above interface, and exchanging the liquid contained in the above flow channel system with another liquid, wherein the liquid in the above-described flow channel system is exchanged with another liquid obtained by eliminating insoluble matters from a measurement solvent, and the change in surface plasmon resonance is then measured in a state where the flow of the liquid has been stopped.
    • 本发明的目的是提供一种测量方法,当使用表面等离子体共振测量装置测量生理活性物质和测试物质之间的特异性结合反应时,可以抑制噪声。 本发明提供了一种用于测量表面等离子体共振的变化的方法,包括:使用表面等离子体共振测量装置,其包括金属膜,用于产生光束的光源,用于允许上述光束 进入金属膜的界面,使得能够在其界面获得全反射条件,并且可以包括各种入射角,包括形成在上述金属膜上的单元的流路系统和光检测 用于通过测量在上述界面全反射的光束强度来检测表面等离子体共振的状态的装置,以及将上述流道系统中所含的液体与另一种液体进行交换,其中上述流道系统 与通过从测量溶剂中除去不溶物质而获得的另一种液体以及表面质膜的变化进行交换 然后在液体的流动停止的状态下测量单次共振。
    • 9. 发明申请
    • Method for measuring the surface plasmon resonance
    • 测量表面等离子体共振的方法
    • US20060187460A1
    • 2006-08-24
    • US11354839
    • 2006-02-16
    • Hirohiko Tsuzuki
    • Hirohiko Tsuzuki
    • G01N21/55
    • G01N21/553
    • It is an object of the present invention to provide a method for obtaining a chromatogram using the SPR analysis method. The present invention provides a method for measuring the change in the surface plasmon resonance by using a surface plasmon resonance measurement device which comprises a metal film, a light source for generating a light beam, an optical system for allowing such a light beam to enter the interface of the metal film so that total reflection conditions can be obtained at the interface thereof and so that various incidence angles can be included, a flow channel system comprising a cell formed on said metal film, and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of the light beam totally reflected at said interface, and exchanging a liquid contained in the flow channel system; wherein the measurement is carried out by supplying a liquid at a liquid amount such that the absolute amount of molecules adsorbed on the metal film surface is lower than the saturated adsorption amount of said molecules on said metal film surface.
    • 本发明的目的是提供使用SPR分析方法获得色谱图的方法。 本发明提供了一种用于通过使用表面等离子体共振测量装置来测量表面等离子体共振的变化的方法,所述表面等离子体共振测量装置包括金属膜,用于产生光束的光源,用于允许这种光束进入 使得能够在其界面处获得全反射条件,并且可以包括各种入射角,包括形成在所述金属膜上的单元的流路系统和用于检测状态的光检测装置 通过测量在所述界面处全反射的光束的强度,并且更换包含在所述流道系统中的液体的表面等离子体共振; 其中所述测量是通过以液体量供给液体来进行的,以使吸附在所述金属膜表面上的分子的绝对量低于所述金属膜表面上的所述分子的饱和吸附量。