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    • 1. 发明授权
    • Double-resonance-absorption microscope
    • 双共振吸收显微镜
    • US06844963B2
    • 2005-01-18
    • US09814125
    • 2001-03-22
    • Yoshinori IketakiMashaaki FujiiTakeshige OmatsuOsamu SatoKimiyoshi Yamamoto
    • Yoshinori IketakiMashaaki FujiiTakeshige OmatsuOsamu SatoKimiyoshi Yamamoto
    • G01J3/44G01N21/64G02B21/16G21K7/00G02B21/00G01J3/30
    • G01J3/4406G01N21/6428G01N21/6458G02B21/16
    • The present invention provides various embodiments of a double-resonance-absorption microscope which realizes a super-resolution by using double resonance absorption. In particular, a double-resonance-absorption microscope includes a light source for a pump light of a wavelength λ1 which excites a sample molecule to a first electronic excited state from a ground state, a light source for an erase light of a wavelength λ2 which excites the sample molecule to a second electronic excited state or a higher excited state from the first electronic excited state, and an overlap component for partially overlapping irradiating areas of the pump light and the erase light with each other. An emission area upon deexcitation of the sample molecule to the ground state from the first electronic excited state is partially inhibited by irradiating the pump light and the erase light through the overlap means. On an optical path of the erase light, a spatial filter is provided which has a condenser lens, a collimate lens, and a pinhole therebetween, and performs condensing of the erase light onto the pinhole by the condenser lens and collimating of the erase light passed through the pinhole into a parallel beam by the collimate lens.
    • 本发明提供了通过使用双共振吸收实现超分辨率的双共振吸收显微镜的各种实施方案。 特别地,双共振吸收显微镜包括用于从基态将样品分子激发到第一电子激发态的波长λ1的泵浦光的光源,波长为λ2的擦除光的光源, 将样品分子从第一电子激发态激发到第二电子激发态或更高的激发态,以及用于使泵浦光和擦除光的照射区域部分重叠的重叠部件。 通过经由重叠装置照射泵浦光和擦除光,部分地抑制从第一电子激发态将样品分子脱离到基态的发射区域。 在擦除光的光路上,提供了具有聚光透镜,准直透镜和针孔之间的空间滤光器,并且通过聚光透镜将擦除光聚焦到针孔上并准直通过的擦除光 通过准直透镜通过针孔进入平行光束。