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1. 发明申请

US20090311680A1 Method for Identifying Useful Proteins of Brewery Yeast 审中-公开
标题翻译：识别啤酒酵母有用蛋白质的方法
公开(公告)号：US20090311680A1
公开(公告)日：2009-12-17
申请号：US12224371
申请日：2007-02-26
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Takeshi Kanamori
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga , Takeshi Kanamori
IPC分类号： C12Q1/68
CPC分类号： C12N15/1034
摘要： The invention relates to a method for identifying a useful protein of brewery yeast. More specifically, the invention relates to (a) cultivating yeast under a predetermined cultivation condition; (b) extracting a protein sample from the cultivation product of the yeast; (c) separating the protein sample by a protein separation means, selecting a target peak or spot, and recovering the target protein or a fragment thereof contained in the peak or spot; (d) determining the amino acid sequence of the target protein; (e) comparing the amino acid sequence determined in step (d) with the amino acid sequence determined in advance based on all or a part of genome sequence information of bottom fermenting yeast; (f) identifying the target protein and the gene encoding the target protein based on the results of comparison; and (g) analyzing functions of the identified gene to identify characters given to the yeast by the gene.
摘要翻译：本发明涉及鉴定啤酒酵母有用蛋白质的方法。更具体地，本发明涉及（a）在预定的培养条件下培养酵母; （b）从酵母的培养产物中提取蛋白质样品; （c）通过蛋白质分离装置分离蛋白质样品，选择目标峰或斑点，并回收峰或点中所含的靶蛋白或其片段; （d）确定靶蛋白的氨基酸序列; （e）基于底部发酵酵母的全部或部分基因组序列信息，比较步骤（d）中确定的氨基酸序列与预先确定的氨基酸序列; （f）基于比较结果鉴定靶蛋白和编码靶蛋白的基因; 和（g）分析所鉴定的基因的功能以鉴定由该基因给予酵母的字符。

2. 发明申请

US20090297657A1 O-acetylhomoserinesulfhydorelace gene and use thereof 审中-公开
标题翻译： O-乙酰高丝氨酸水解酶基因及其用途
公开(公告)号：US20090297657A1
公开(公告)日：2009-12-03
申请号：US11887077
申请日：2006-08-21
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/022 , C07H21/04 , C07K14/39 , C12N15/63 , C12N1/19 , C12Q1/68
CPC分类号： C12C12/006 , C12C12/004 , C12G1/0203 , C12N9/1085
摘要： The present invention relates to a brewery yeast having controlled hydrogen sulfide-producing capability, a process for producing alcoholic beverages with controlled hydrogen sulfide amount. More particularly, the present invention relates to a yeast whose hydrogen sulfide-producing capability that increases the product flavor is controlled by enhancing the expression level of MET17 gene encoding brewery yeast O-acetylhomoserinesulfhydorelace Met17p, particularly non-ScMET17 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
摘要翻译：本发明涉及一种具有受硫化氢生产能力的啤酒酵母，一种生产具有可控硫化氢含量的酒精饮料的方法。更具体地说，本发明涉及通过提高编码啤酒酵母O-乙酰高丝氨酸亚磺基水合酶Met17p的MET17基因，特别是对大啤酒酵母特异性的非ScMET17基因的表达水平来控制增加产品风味的硫化氢生产能力的酵母，以及用所述酵母生产酒精饮料的方法。

3. 发明申请

US20090269437A1 Glycerol channel gene and use thereof 审中-公开
标题翻译：甘油通道基因及其用途
公开(公告)号：US20090269437A1
公开(公告)日：2009-10-29
申请号：US11990930
申请日：2006-08-31
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12N1/19 , C12N15/11 , C07K14/00 , C12N15/00 , C12Q1/68 , C12C11/09 , C12G1/073 , A23L1/185 , A23L1/202
CPC分类号： C07K14/395 , C12C12/004 , C12C12/006 , C12G1/0203 , C12G3/02
摘要： The present invention relates to a glycerol channel gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent body and mellowness, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing glycerol, which contribute to body and mellowness of products, was controlled by controlling expression level of FPS1 gene encoding brewery yeast glycerol channel Fps1p, particularly non-ScFPS1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.
摘要翻译：本发明涉及一种甘油通道基因及其用途，具体地说，一种具有优异身体和醇厚度的啤酒酵母生产酒精饮料，使用酵母生产的含酒精饮料，生产酒精饮料的方法。更具体地说，本发明涉及通过控制编码啤酒酵母甘油通道Fps1p的FPS1基因的表达水平，特别是对大啤酒特异性的非ScFPS1基因来控制有助于产物的身体和醇味的甘油的生产能力的酵母酵母，以及用酵母生产酒精饮料的方法。

4. 发明申请

US20090246316A1 Esterase gene and use thereof 审中-公开
标题翻译：酯酶基因及其用途
公开(公告)号：US20090246316A1
公开(公告)日：2009-10-01
申请号：US11991065
申请日：2006-09-12
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12Q1/68 , C12N9/16 , C12N15/74 , C12N1/19 , G01N33/569 , C12C11/09 , C12G1/073 , C12Q1/44 , C12Q3/00 , C12R1/645
CPC分类号： C12N9/18 , C12C12/004 , C12C12/006 , C12G1/0203 , C12P7/62
摘要： The present invention relates to a esterase gene and its uses, specifically, a brewery yeast producing alcoholic beverages with excellent aroma and flavor, alcoholic beverages produced using the yeast, a process for producing the alcoholic beverages. More particularly, the present invention relates to a yeast whose capability of producing ester, which contribute to aroma and flavor of products, was controlled by regulating expression level of IAH1 gene encoding brewery yeast esterase Iah1p, particularly nonScIAH1 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with the yeast.
摘要翻译：本发明涉及一种酯酶基因及其用途，具体地说，一种酿造具有优良芳香和风味的酒精饮料的啤酒酵母，使用该酵母生产的含酒精饮料，一种生产该酒精饮料的方法。更具体地，本发明涉及通过调节编码啤酒酵母酯酶Iah1p的IAH1基因，特别是对啤酒酵母特异性的非scIAH1基因的表达水平来控制有助于产物的香味和风味的酵母的酵母，以及涉及一种用酵母生产酒精饮料的方法。

5. 发明申请

US20090175983A1 Gene capable of improving low temperature fermentability and/or low temperature resistance and use thererof 审中-公开
标题翻译：能够改善低温发酵能力和/或耐低温性能的基因
公开(公告)号：US20090175983A1
公开(公告)日：2009-07-09
申请号：US11990942
申请日：2006-08-31
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12C1/00 , C07H21/04 , C07K14/37 , C12N15/63 , C12N1/19 , C12Q1/68 , C12G1/00
CPC分类号： C07K14/395 , C12C11/003 , C12C12/004 , C12C12/006 , C12G1/0203 , C12G2200/11 , C12R1/85
摘要： The present invention relates to a gene capable of improving low temperature performance (low temperature fermentability and/or low temperature resistance) and use thereof, in particular, to a brewery yeast with superior low temperature performance, alcoholic beverages produced using said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose low temperature performance is improved by amplifying expression level of DLT1 gene encoding Dltlp capable of improving low temperature performance of a brewery yeast, especially non-ScDLT1 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages using said yeast.
摘要翻译：本发明涉及能够改善低温性能（低温发酵性和/或耐低温性）及其使用，特别是具有优异的低温性能的啤酒酵母，使用所述酵母生产的含酒精饮料的基因和生产所述饮料的方法。更具体地说，本发明涉及通过扩增能够提高啤酒酵母的低温性能的特别是非特异性的不含ScDLT1基因的编码Dltlp的DLT1基因的表达水平来提高其低温性能的酵母，以及涉及使用所述酵母生产酒精饮料的方法。

6. 发明申请

US20090117227A1 GENE ENCODING TREHALOSE-6-PHOSPHATE PHOSPHATASE AND USE THEREOF 审中-公开
标题翻译：基因编码TREHALOSE-6磷酸酯磷酸酶及其用途
公开(公告)号：US20090117227A1
公开(公告)日：2009-05-07
申请号：US12279376
申请日：2007-02-01
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/00 , C07H21/04 , C07K14/395 , A23L1/202 , C12Q1/68 , C12N15/63 , C12N1/19
CPC分类号： C12Q1/42 , C12C12/004 , C12C12/006 , C12G1/0203 , C12N9/16 , C12Q1/02 , C12R1/85 , C12Y301/03012 , G01N2333/39
摘要： The present invention relates to a gene encoding trehalose-6-phosphate phosphatase and use thereof, in particular, a yeast for practical use with superior resistance property to dryness and/or low-temperature storage, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose resistance property to dryness and/or resistance property to low-temperature storage is enhanced by amplifying expression level of TPS2 gene encoding a trehalose-6-phosphate phosphatase Tps2p in brewer's yeast, especially non-ScTPS2 gene specific to a lager brewing yeast and to a method for producing alcoholic beverages with said yeast, etc.
摘要翻译：本发明涉及编码海藻糖-6-磷酸磷酸酶的基因及其用途，特别是用于实际使用的具有优异的耐干性和/或低温储存性的酵母，用所述酵母生产的含酒精饮料的方法和方法用于生产所述饮料。更具体地说，本发明涉及通过扩增酿酒酵母中编码海藻糖-6-磷酸磷酸酶Tps2p的TPS2基因的表达水平，特别是非抑制性酵母，其酵母对抗低温贮藏的耐干性和/ 特异于啤酒酿造酵母的-ScTPS2基因和用酵母等生产酒精饮料的方法

7. 发明申请

US20090053359A1 AMMONIA TRANSPORTER GENE AND USE THEREOF 审中-公开
标题翻译：氨基酸运输基因及其使用
公开(公告)号：US20090053359A1
公开(公告)日：2009-02-26
申请号：US12279080
申请日：2006-12-05
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/00 , C07H21/00 , C07K14/00 , C12N15/00 , C12N15/63 , C12N1/19 , C12Q1/02 , C12Q1/68 , C12C11/00
CPC分类号： C07K14/435 , C12C12/004 , C12C12/006 , C12G1/0203
摘要： The present invention relates to ammonia transporter genes and use thereof. The invention relates in particular to a brewer's yeast which shows enhanced ammonia assimilation, alcoholic beverages produced using such yeast, and a method of producing such alcoholic beverages. More specifically, the invention relates to the MEP3 gene which codes for the ammonia transporter Mep3 in brewer's yeast, particularly to a yeast which can control the ammonia assimilation ability by controlling the level of expression of the nonScMEP3 gene or ScMEP3 gene characteristic to beer yeast and to a method of producing alcoholic beverages using such yeast.
摘要翻译：本发明涉及氨转运蛋白基因及其应用。本发明特别涉及显示增强的氨同化的酿酒酵母，使用这种酵母生产的含酒精饮料，以及生产这种酒精饮料的方法。更具体地，本发明涉及编码啤酒酵母中氨转运蛋白Mep3的MEP3基因，特别是涉及通过控制啤酒酵母特有的nonScMEP3基因或ScMEP3基因的表达水平来控制氨同化能力的酵母，涉及使用这种酵母生产酒精饮料的方法。

8. 发明申请

US20100129491A1 Tryptophan transporter gene and use thereof 审中-公开
标题翻译：色氨酸转运蛋白基因及其用途
公开(公告)号：US20100129491A1
公开(公告)日：2010-05-27
申请号：US11991162
申请日：2006-08-31
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C07K14/00 , C12N15/11 , C12N15/00 , C12N1/19 , C12Q1/68 , C12Q1/02 , C12C11/09 , C12C11/00 , C12G1/022
CPC分类号： C07K14/395 , C12C11/003 , C12C12/004 , C12C12/006 , C12G1/0203 , C12G2200/11
摘要： The present invention relates to a tryptophan transporter gene and to uses of the gene. The invention relates in particular to a brewer's yeast which can control the tryptophan assimilation ability, alcoholic beverages produced using such yeast, and a method of producing such alcoholic beverages. More specifically, the invention relates to a yeast which can control the tryptophan assimilation ability by controlling the level of expression of the TAT2 gene which codes for the tryptophan transporter Tat2p in brewer's yeast, particularly of the nonScTAT2 gene characteristic to beer yeast, and to a method of producing alcoholic beverages using such yeast.
摘要翻译：本发明涉及色氨酸转运蛋白基因和该基因的用途。本发明特别涉及能够控制色氨酸同化能力的啤酒酵母，使用这种酵母产生的酒精饮料，以及这种酒精饮料的制造方法。更具体地，本发明涉及通过控制编码啤酒酵母中的色氨酸转运蛋白Tat2p的特异性TAT2基因的表达水平，特别是啤酒酵母特有的nonScTAT2基因，能够控制色氨酸同化能力的酵母。使用这种酵母生产酒精饮料的方法。

9. 发明申请

US20090246317A1 ACYL-COA: ETHANOL O-ACYLTRANSFERASE/ESTERASE GENE AND USE THEREOF 审中-公开
标题翻译： ACYL-COA：乙醇O-酰基转移酶/酯酶基因及其用途
公开(公告)号：US20090246317A1
公开(公告)日：2009-10-01
申请号：US12095242
申请日：2006-12-01
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12C12/00 , C07H21/00 , C07H21/04 , C12N9/10 , C12N15/63 , C12N1/19 , C12Q1/68 , C12Q1/02 , C12H1/00 , C12G1/073 , C12H1/14
CPC分类号： C12N9/1029 , C12N9/18
摘要： The present invention relates to an acyl-CoA: ethanol O-acyltransferase/esterase gene and use thereof, in particular, a brewery yeast for producing alcoholic beverages with superior flavor, alcoholic beverages produced with said yeast, and a method for producing said beverages. More particularly, the present invention relates to a yeast, whose capability of producing ester, which contribute to aroma and flavor of products, is controlled by regulating expression level of EHT1 gene encoding a protein (Eht1p) that is an acyl-CoA: ethanol O-acyltransferase/esterase in a brewery yeast, especially the nonScEHT1 gene specific to a lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
摘要翻译：本发明涉及酰基-CoA：乙醇O-酰基转移酶/酯酶基因及其用途，特别是用于生产具有优良风味的酒精饮料的啤酒酵母，用所述酵母生产的酒精饮料及其制备方法。更具体地，本发明涉及通过调节编码作为酰基-CoA：乙醇O的蛋白质（Eht1p）的EHT1基因的表达水平来控制其产生产生香气和风味的酯的酵母乙酰转移酶/酯酶，特别是对啤酒酿造酵母特异性的nonScEHT1基因，以及用所述酵母生产酒精饮料的方法。

10. 发明申请

US20090053358A1 Sulfate Ion Transporter Gene and Use Thereof 审中-公开
标题翻译：硫酸根离子转运蛋白基因及其用途
公开(公告)号：US20090053358A1
公开(公告)日：2009-02-26
申请号：US11922219
申请日：2006-06-16
申请人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
发明人： Yoshihiro Nakao , Yukiko Kodama , Tomoko Shimonaga
IPC分类号： C12G1/022 , C12N15/11 , C07K14/00 , C12N15/00 , C12Q1/02 , C12Q1/68 , C12N1/19 , C12C11/00
CPC分类号： C07K14/395
摘要： The present invention relates to a brewery yeast having enhanced sulfite-producing capability, a process for producing alcoholic beverages with enhanced sulfite amount. More particularly, the present invention relates to a yeast whose sulfite-producing capability that contribute to the product flavor is enhanced by increasing the expression level of SUL2 gene encoding brewery yeast sulfate ion transporter Sul2p, particularly non-ScSUL2 gene specific to lager brewing yeast, and to a method for producing alcoholic beverages with said yeast.
摘要翻译：本发明涉及具有提高的亚硫酸盐生产能力的啤酒酵母，一种生产亚硫酸盐含量增加的酒精饮料的方法。更具体地说，本发明涉及通过增加编码啤酒酵母硫酸盐离子转运体Sul2p的SUL2基因，特别是对大啤酒酵母特异性的非ScSUL2基因的表达水平来提高有助于产品风味的亚硫酸盐产生能力的酵母，以及用所述酵母生产酒精饮料的方法。

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