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1. 发明申请

US20120003669A1 Immunohistochemical staining method and immunohistochemical staining apparatus 审中-公开
标题翻译：免疫组织化学染色法和免疫组化染色法
公开(公告)号：US20120003669A1
公开(公告)日：2012-01-05
申请号：US13151730
申请日：2011-06-02
申请人： Yoshihiro MINAMIYA , Hiroshi Toda , Jun-ichi Ogawa , Yoichi Akagami , Masami Kagaya
发明人： Yoshihiro MINAMIYA , Hiroshi Toda , Jun-ichi Ogawa , Yoichi Akagami , Masami Kagaya
IPC分类号： G01N33/53 , C12M1/34 , G01N21/64
CPC分类号： G01N33/58 , G01N1/30
摘要： An immunohistochemical staining apparatus for carrying out immunohistochemical staining includes a sample chamber (1) in which a primary sample (s1) comprised of a tissue specimen (t) to which a primary antibody (Ig1) is applied is disposed between a sample-side electrode (11) and an opposed electrode (12). The sample chamber (1) has first stirring means for noncontactlessly stirring the primary sample (s1) by applying for a first prescribed time period an alternating electric field adjusted so that the opposed electrode (12) is minus with respect to the sample-side electrode (11), and second stirring means for noncontactlessly stirring a secondary sample (s2) comprised of the noncontactlessly-stirred primary sample (s1) to which a labeled secondary antibody (Ig2) is applied by applying for a second prescribed time period an alternating electric field adjusted so that the opposed electrode (12) is minus with respect to the sample-side electrode (11).
摘要翻译：用于进行免疫组织化学染色的免疫组织化学染色装置包括：样品室（1），其中将应用了一次抗体（Ig1）的组织标本（t）组成的初级样品（s1）设置在样品侧电极（11）和相对电极（12）。样品室（1）具有第一搅拌装置，用于通过施加第一规定时间周期不间断地搅拌初级样品（s1），进行交变电场调整，使得相对电极（12）相对于样品侧电极为负（11）和第二搅拌装置，用于非接触地搅拌由非接触搅拌的初级样品（s1）组成的二次样品（s2），通过施加第二规定时间段，施加标记的二次抗体（Ig2），交替电场相对电极（12）相对于取样侧电极（11）为负。

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