专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US07393668B2 Method of collecting highly pure polyhydroxyalkanoate from microbial cells 有权
标题翻译：从微生物细胞中收集高纯度聚羟基链烷酸酯的方法
公开(公告)号：US07393668B2
公开(公告)日：2008-07-01
申请号：US10541389
申请日：2004-01-20
申请人： Yoshifumi Yanagita , Noriko Ogawa , Yasuyoshi Ueda , Fumio Osakada , Keiji Matsumoto
发明人： Yoshifumi Yanagita , Noriko Ogawa , Yasuyoshi Ueda , Fumio Osakada , Keiji Matsumoto
IPC分类号： C12P7/62 , C12N9/00
CPC分类号： C12P7/625
摘要： The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles.The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.
摘要翻译：本发明的目的是提供一种分离纯化PHA的方法，而不会导致分子量的严重降低以获得高产率的高纯度PHA，其包括从培养的PHA有效除去PHA颗粒以外的细胞成分含微生物细胞。本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。根据本发明的回收PHA的方法是通过对含PHA的微生物细胞的水性悬浮液进行物理破坏处理和低温碱添加来有效地破坏细胞以回收PHA的方法，然后用酶和/或表面活性剂处理PHA。此外，通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集，可以扩大PHA的粒径。

2. 发明申请

US20060084161A1 Method of collecting highly pure polyhydroxyalkanoate from microbial cells 有权
标题翻译：从微生物细胞中收集高纯度聚羟基链烷酸酯的方法
公开(公告)号：US20060084161A1
公开(公告)日：2006-04-20
申请号：US10541389
申请日：2004-01-20
申请人： Yoshifumi Yanagita , Noriko Ogawa , Yasuyoshi Ueda , Fumio Osakada , Keiji Matsumoto
发明人： Yoshifumi Yanagita , Noriko Ogawa , Yasuyoshi Ueda , Fumio Osakada , Keiji Matsumoto
IPC分类号： C12N9/00
CPC分类号： C12P7/625
摘要： The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles. The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.
摘要翻译：本发明的目的是提供一种分离纯化PHA的方法，而不会导致分子量的严重降低以获得高产率的高纯度PHA，其包括从培养的PHA有效除去PHA颗粒以外的细胞成分含微生物细胞。本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。根据本发明的回收PHA的方法是通过对含PHA的微生物细胞的水性悬浮液进行物理破坏处理和低温碱添加来有效地破坏细胞以回收PHA的方法，然后用酶和/或表面活性剂处理PHA。此外，通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集，可以扩大PHA的粒径。

3. 发明授权

US07435567B2 Method for degradation of nucleic acids and use thereof 有权
标题翻译：核酸降解方法及其用途
公开(公告)号：US07435567B2
公开(公告)日：2008-10-14
申请号：US11070083
申请日：2005-03-03
申请人： Fumio Osakada , Yoshifumi Yanagida , Yasuyoshi Ueda
发明人： Fumio Osakada , Yoshifumi Yanagida , Yasuyoshi Ueda
IPC分类号： C12P7/62
CPC分类号： C12M47/06 , C12P7/62
摘要： The present invention provides a method to efficiently degrade nucleic acids, which result in a viscosity increase of the solution thereof on the occasion of decomposition or solubilization of microbial cells, in an easy and simple manner in the step of recovering various useful substance produced by a microorganism, and a use thereof. The product recovery method of the present invention make the product recovery from within microbial cells with ease under relatively mild conditions, because, by bringing living microbial cells into contact with a little amount of hypochlorous acid or a salt thereof, autodigestion of nucleic acids is induced and following decomposition of microbial cells or viscosity increase of the solution thereof after dissolution is inhibited. The method of the present invention is particularly preferred in recovering polyhydroxyalkanoates, which are biodegradable polymers, from microbial cells.
摘要翻译：本发明提供了一种在回收各种有用物质的步骤中以容易且简单的方式有效降解核酸的方法，其在微生物细胞的分解或溶解的情况下导致其溶液的粘度增加，微生物及其用途。本发明的产品回收方法使得在相对温和的条件下容易从微生物细胞中回收产物，因为通过使活的微生物细胞与少量的次氯酸或其盐接触，诱导了核酸的自身消化并且随着微生物细胞的分解或溶解后其溶液的粘度增加被抑制。本发明的方法特别优选从微生物细胞回收可生物降解的聚合物的聚羟基链烷酸酯。

4. 发明授权

US07098298B2 Method for producing polyhydroxyalkanoate crystal 有权
标题翻译：聚羟基链烷酸酯晶体的制备方法
公开(公告)号：US07098298B2
公开(公告)日：2006-08-29
申请号：US10992133
申请日：2004-11-19
申请人： Koichi Kinoshita , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Christine Cearley , Kenneth Yee , Isao Noda
发明人： Koichi Kinoshita , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Christine Cearley , Kenneth Yee , Isao Noda
IPC分类号： C08F6/00
CPC分类号： C12P7/625 , C08G63/06 , C08G63/90
摘要： The present invention provides a method for conveniently obtaining a biodegradable polyhydroxyalkanoate by a solvent extraction method. A method for producing a polyhydroxyalkanoate crystal comprises precipitating a polyhydroxyalkanoate crystal using a monohydric alcohol having 4 to 10 carbon atoms as a extraction solvent, keeping a polyhydroxyalkanoate solution containing 0.1 to 10% by weight of water relative to the total amount of the solution warm at 70° C. or higher, and cooling the solution to below 70° C.
摘要翻译：本发明提供了通过溶剂萃取方法方便地获得生物可降解聚羟基链烷酸酯的方法。聚羟基链烷酸酯晶体的制造方法包括使用具有4〜10个碳原子的一元醇作为提取溶剂使聚羟基链烷酸酯晶体析出，保持含有0.1〜10重量％的水的聚羟基链烷酸酯溶液相对于温度为 70℃以上，将溶液冷却至70℃以下。

5. 发明申请

US20050239998A1 Method for producing polyhydroxyalkanoate 审中-公开
标题翻译：聚羟基链烷酸酯的制备方法
公开(公告)号：US20050239998A1
公开(公告)日：2005-10-27
申请号：US11070188
申请日：2005-03-03
申请人： Koichi Kinoshita , Yoshifumi Yanagida , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Cearley , Kenneth Yee , Isao Noda
发明人： Koichi Kinoshita , Yoshifumi Yanagida , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Cearley , Kenneth Yee , Isao Noda
IPC分类号： C08G63/02 , C08G63/06 , C08G63/89 , C12P7/62
CPC分类号： C08G63/89 , C08G63/06 , C12P7/625
摘要： The present invention provides a method for obtaining a biodegradable polyhydroxyalkanoate by a solvent extraction method without causing a significant molecular weight decrease. The present invention relates to a method for producing a polyhydroxyalkanoate which comprises extracting a polyhydroxyalkanoate from a polyhydroxyalkanoate-containing biomass having the water content of 5% by weight or less using an extraction solvent, crystallizing, and recovering the resultant.
摘要翻译：本发明提供了一种通过溶剂萃取法获得可生物降解的聚羟基链烷酸酯的方法，而不会导致明显的分子量降低。本发明涉及一种生产聚羟基链烷酸酯的方法，该方法包括使用萃取溶剂从具有5重量％以下含水量的聚羟基链烷酸酯的生物质提取聚羟基链烷酸酯，结晶并回收所得聚合物。

6. 发明申请

US20050222373A1 Method for producing polyhydroxyalkanoate crystal 失效
标题翻译：聚羟基链烷酸酯晶体的制备方法
公开(公告)号：US20050222373A1
公开(公告)日：2005-10-06
申请号：US10992132
申请日：2004-11-19
申请人： Koichi Kinoshita , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Cearley , Kenneth Yee , Isao Noda
发明人： Koichi Kinoshita , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Cearley , Kenneth Yee , Isao Noda
IPC分类号： C08G67/00
CPC分类号： C08G63/90 , C08G63/06
摘要： The present invention provides a method for easily obtaining a biodegradable polyhydroxyalkanoate by a solvent extraction method. A method for producing a polyhydroxyalkanoate crystal which comprises mixing a solution of a polyhydroxyalkanoate in a good solvent with a poor solvent at 50 to 130° C. to precipitate a polyhydroxyalkanoate.
摘要翻译：本发明提供了一种通过溶剂萃取方法容易地获得可生物降解的聚羟基链烷酸酯的方法。一种制备聚羟基链烷酸酯晶体的方法，其包括在50-130℃下将聚羟基链烷酸酯在良溶剂中的溶液与不良溶剂混合以沉淀聚羟基链烷酸酯。

7. 发明申请

US20050228168A1 Method for producing polyhydroxyalkanoate crystal 有权
标题翻译：聚羟基链烷酸酯晶体的制备方法
公开(公告)号：US20050228168A1
公开(公告)日：2005-10-13
申请号：US10992133
申请日：2004-11-19
申请人： Koichi Kinoshita , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Cearley , Kenneth Yee , Isao Noda
发明人： Koichi Kinoshita , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Cearley , Kenneth Yee , Isao Noda
IPC分类号： C08F6/00 , C08G63/06 , C08G63/90 , C12P7/62
CPC分类号： C12P7/625 , C08G63/06 , C08G63/90
摘要： The present invention provides a method for conveniently obtaining a biodegradable polyhydroxyalkanoate by a solvent extraction method. A method for producing a polyhydroxyalkanoate crystal comprises precipitating a polyhydroxyalkanoate crystal using a monohydric alcohol having 4 to 10 carbon atoms as a extraction solvent, keeping a polyhydroxyalkanoate solution containing 0.1 to 10% by weight of water relative to the total amount of the solution warm at 70° C. or higher, and cooling the solution to below 70° C.
摘要翻译：本发明提供一种通过溶剂萃取方法方便地获得生物可降解的聚羟基链烷酸酯的方法。聚羟基链烷酸酯晶体的制造方法包括使用具有4〜10个碳原子的一元醇作为提取溶剂使聚羟基链烷酸酯晶体析出，保持含有0.1〜10重量％的水的聚羟基链烷酸酯溶液相对于温度为 70℃以上，将溶液冷却至70℃以下。

8. 发明申请

US20060105440A1 Method of producing polyhydroxyalkanoate 审中-公开
标题翻译：聚羟基链烷酸酯的制备方法
公开(公告)号：US20060105440A1
公开(公告)日：2006-05-18
申请号：US11237686
申请日：2005-09-29
申请人： Koichi Kinoshita , Yoshifumi Yanagida , Fumio Osakada , Yasuyoshi Ueda
发明人： Koichi Kinoshita , Yoshifumi Yanagida , Fumio Osakada , Yasuyoshi Ueda
IPC分类号： C12P7/62
CPC分类号： C12P7/62 , C08G63/89
摘要： The present invention has its object to provide a method of producing PHA by extracting, separating, and purifying PHA from biomass containing PHA having an weight average molecular weight of more than 2,000,000, by which smooth stirring can be carried out during extraction and filterability of a residue becomes good to thereby efficiently produce PHA with good operability. In the present invention, PHA is extracted, separated, and purified from biomass by a method of producing polyhydroxyalkanoate by extracting and isolating polyhydroxyalkanoate by using an aprotic organic solvent from biomass containing polyhydroxyalkanoate having a weight average molecular weight of more than 2,000,000, which comprises decreasing the weight average molecular weight of the polyhydroxyalkanoate through any one of the following treatments: (a) the biomass is heated at 40 to 500° C. before addition of the aprotic organic solvent; (b) the biomass is heated at 40 to 500° C. before addition of the aprotic organic solvent, and is further heated at 40 to 200° C. in the aprotic organic solvent; (c) the biomass is heated at 40 to 500° C. before addition of the aprotic organic solvent, and is further heated at 40 to 200° C. in the aprotic organic solvent in the presence of water and/or an alcohol; (d) the biomass is not heated before addition of the aprotic organic solvent, but is heated at 40 to 200° C. in the aprotic organic solvent; and (e) the biomass is not heated before addition of the aprotic organic solvent, but is heated at 40 to 200° C. in the aprotic organic solvent in the presence of water and/or an alcohol.
摘要翻译：本发明的目的是提供一种通过从重均分子量大于2,000,000的PHA的生物质中提取，分离和纯化PHA来生产PHA的方法，通过该方法可以在提取过程中进行平稳的搅拌和过滤性残留物变得良好，从而有效地产生具有良好可操作性的PHA。在本发明中，通过使用含有重均分子量大于2,000,000的聚羟基链烷酸酯的生物质的非质子有机溶剂提取和分离聚羟基链烷酸酯的方法，从生物质中提取，分离和纯化PHA，其包括降低通过以下任何一种处理，聚羟基链烷酸酯的重均分子量：（a）在加入非质子有机溶剂之前，将生物质在40-500℃下加热; （b）在加入非质子有机溶剂之前将生物质在40-500℃加热，并在非质子有机溶剂中在40〜200℃下进一步加热; （c）在加入非质子有机溶剂之前将生物质加热至40-500℃，并在水和/或醇存在下，在非质子有机溶剂中在40〜200℃下进一步加热; （d）在加入非质子有机溶剂之前，不加热生物质，但在非质子有机溶剂中在40〜200℃下加热生物质; 和（e）生物质在加入非质子有机溶剂之前不被加热，但在水和/或醇的存在下，在非质子传递的有机溶剂中在40-200℃下加热。

9. 发明申请

US20050196827A1 Method for degradation of nucleic acids and use thereof 有权
标题翻译：核酸降解方法及其用途
公开(公告)号：US20050196827A1
公开(公告)日：2005-09-08
申请号：US11070083
申请日：2005-03-03
申请人： Fumio Osakada , Yoshifumi Yanagida , Yasuyoshi Ueda
发明人： Fumio Osakada , Yoshifumi Yanagida , Yasuyoshi Ueda
IPC分类号： C12P7/62
CPC分类号： C12M47/06 , C12P7/62
摘要： The present invention provides a method to efficiently degrade nucleic acids, which result in a viscosity increase of the solution thereof on the occasion of decomposition or solubilization of microbial cells, in an easy and simple manner in the step of recovering various useful substance produced by a microorganism, and a use thereof. The product recovery method of the present invention make the product recovery from within microbial cells with ease under relatively mild conditions, because, by bringing living microbial cells into contact with a little amount of hypochlorous acid or a salt thereof, autodigestion of nucleic acids is induced and following decomposition of microbial cells or viscosity increase of the solution thereof after dissolution is inhibited. The method of the present invention is particularly preferred in recovering polyhydroxyalkanoates, which are biodegradable polymers, from microbial cells.
摘要翻译：本发明提供了一种在回收各种有用物质的步骤中以容易且简单的方式有效降解核酸的方法，其在微生物细胞的分解或溶解的情况下导致其溶液的粘度增加，微生物及其用途。本发明的产品回收方法使得在相对温和的条件下容易从微生物细胞中回收产物，因为通过使活的微生物细胞与少量的次氯酸或其盐接触，诱导了核酸的自身消化并且随着微生物细胞的分解或溶解后其溶液的粘度增加被抑制。本发明的方法特别优选从微生物细胞回收可生物降解的聚合物的聚羟基链烷酸酯。

10. 发明授权

US07153928B2 Method for producing polyhydroxyalkanoate crystal 失效
标题翻译：聚羟基链烷酸酯晶体的制备方法
公开(公告)号：US07153928B2
公开(公告)日：2006-12-26
申请号：US10992132
申请日：2004-11-19
申请人： Koichi Kinoshita , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Christine Cearley , Kenneth Yee , Isao Noda
发明人： Koichi Kinoshita , Fumio Osakada , Yasuyoshi Ueda , Karunakaran Narasimhan , Angella Christine Cearley , Kenneth Yee , Isao Noda
IPC分类号： C08F6/00
CPC分类号： C08G63/90 , C08G63/06
摘要： The present invention provides a method for easily obtaining a biodegradable polyhydroxyalkanoate by a solvent extraction method. A method for producing a polyhydroxyalkanoate crystal comprises mixing a solution of a polyhydroxyalkanoate in a good solvent with a poor solvent at 50 to 130° C. to precipitate a polyhydroxyalkanoate.
摘要翻译：本发明提供了一种通过溶剂萃取方法容易地获得可生物降解的聚羟基链烷酸酯的方法。制备聚羟基链烷酸酯晶体的方法包括在50-130℃下将聚羟基链烷酸酯在良溶剂中的溶液与不良溶剂混合以沉淀聚羟基链烷酸酯。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式