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1. 发明授权

US07713700B2 Method of amplifying nucleic acids, reagent kit for amplifying nucleic acids, method of detecting single nucleotide polymorphism, and reagent kit for detecting single nucleotide polymorphism 失效
标题翻译：核酸扩增方法，核酸扩增试剂盒，单核苷酸多态性检测方法，单核苷酸多态性检测试剂盒
公开(公告)号：US07713700B2
公开(公告)日：2010-05-11
申请号：US12062215
申请日：2008-04-03
申请人： Yasushi Shigemori , Takehiko Shibata , Tsutomu Mikawa , Michio Oishi , Osamu Ohara
发明人： Yasushi Shigemori , Takehiko Shibata , Tsutomu Mikawa , Michio Oishi , Osamu Ohara
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q1/6858 , C12Q2521/507
摘要： An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from Thermus thermophilus, and a modified RecA protein obtained by modification of the RecA protein and having a function similar to that of the RecA protein, and carrying out PCR.
摘要翻译：本发明的目的是提供用于扩增所需核酸同时抑制PCR反应中副产物扩增的核酸扩增方法，用于核酸扩增的试剂盒，检测单核苷酸多态性以检测单核苷酸的方法在PCR反应中抑制副产物扩增的多态性，以及用于检测单核苷酸多态性的试剂盒。通过PCR扩增核酸的方法的特征在于在反应溶液中混合含有至少一种源自Thermus thermophilus的RecA蛋白的同源重组蛋白，以及通过修饰RecA蛋白获得的经修饰的RecA蛋白，并且具有功能类似于RecA蛋白，并进行PCR。

2. 发明申请

US20050260631A1 Method of amplifying nucleic acids, reagent kit for amplifying nucleic acids, method of detecting single nucleotide polymorphism, and reagent kit for detecting single nucleotide polymorphism 审中-公开
标题翻译：核酸扩增方法，核酸扩增试剂盒，单核苷酸多态性检测方法，单核苷酸多态性检测试剂盒
公开(公告)号：US20050260631A1
公开(公告)日：2005-11-24
申请号：US11081949
申请日：2005-03-17
申请人： Yasushi Shigemori , Takehiko Shibata , Tsutomu Mikawa , Michio Oishi , Osamu Ohara
发明人： Yasushi Shigemori , Takehiko Shibata , Tsutomu Mikawa , Michio Oishi , Osamu Ohara
IPC分类号： C12N15/09 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q1/6858 , C12Q2521/507
摘要： An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from Thermus thermophilus, and a modified RecA protein obtained by modification of the RecA protein and having a function similar to that of the RecA protein, and carrying out PCR.
摘要翻译：本发明的目的是提供用于扩增所需核酸同时抑制PCR反应中副产物扩增的核酸扩增方法，用于核酸扩增的试剂盒，检测单核苷酸多态性以检测单核苷酸的方法在PCR反应中抑制副产物扩增的多态性，以及用于检测单核苷酸多态性的试剂盒。通过PCR扩增核酸的方法的特征在于在反应溶液中混合含有至少一种源自Thermus thermophilus的RecA蛋白的同源重组蛋白，以及通过修饰RecA蛋白获得的经修饰的RecA蛋白，并且具有功能类似于RecA蛋白，并进行PCR。

3. 发明授权

US06849410B2 Method for detecting DNA polymorphism applying triple strand DNA formation technique 失效
标题翻译：检测应用三链DNA形成技术的DNA多态性的方法
公开(公告)号：US06849410B2
公开(公告)日：2005-02-01
申请号：US09989526
申请日：2001-11-21
申请人： Yasushi Shigemori , Michio Oishi , Osamu Ohara
发明人： Yasushi Shigemori , Michio Oishi , Osamu Ohara
IPC分类号： C12N15/09 , C07H21/02 , C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6839 , C12Q2537/119 , C12Q2527/107 , C12Q2521/507
摘要： An objective of this invention is to provide a method for detecting DNA polymorphism that has high sensitivity and efficiency and does not need long DNA searching region.A homologous recombination protein RecA makes partial triple strand DNA from target double DNA and oligonucleotide probe complementary to the DNA. The triple strand DNA maintains stable triple strand DNA after RecA protein is removed. The present inventors found that the thermostability of triple strand DNA changes greatly when there is a mismatch between target DNA and oligonucleotide probe because of the existence of polymorphism in the target DNA. Utilizing this change of thermostability, efficient detection of polymorphism in labeled DNA is possible by examining whether oligonucleotide probe is released and the triple strand DNA is solved after heat treatment of triple strand DNA formed using homologous recombination protein.
摘要翻译：本发明的目的是提供一种检测DNA多态性的方法，其具有高灵敏度和高效性并且不需要长的DNA搜索区。同源重组蛋白RecA使得来自靶双重DNA的部分三链DNA和与DNA互补的寡核苷酸探针。在除去RecA蛋白后，三链DNA保持稳定的三链DNA。本发明人发现，由于目标DNA中存在多态性，当靶DNA和寡核苷酸探针之间存在错配时，三链DNA的热稳定性变化很大。利用这种热稳定性的变化，可以通过检查寡核苷酸探针是否被释放并且在使用同源重组蛋白形成的三链DNA热处理后求解三链DNA来有效地检测标记的DNA中的多态性。

4. 发明授权

US07022501B1 Ligation of double-stranded DNAs 失效
标题翻译：双链DNA的连接
公开(公告)号：US07022501B1
公开(公告)日：2006-04-04
申请号：US09607361
申请日：2000-06-30
申请人： Yasushi Shigemori , Michio Oishi
发明人： Yasushi Shigemori , Michio Oishi
IPC分类号： C12P19/34 , C12P21/06 , C12N15/64 , C12N9/00 , C22N1/20
CPC分类号： C12N15/10
摘要： The present invention relates to a DNA ligation method. Specifically, a DNA complex comprising a three-stranded structure in each of the ends of double-stranded DNAs, one of which has single-stranded regions at the end, and the other of which does not have single-stranded regions at the end, is formed under the presence of a homologous recombinant protein. Moreover, according to needs, the DNA complex is introduced into host cells, and said cells are cultured.
摘要翻译：本发明涉及DNA连接方法。具体而言，在双链DNA的各末端具有三链结构的DNA复合体，其中一个末端具有单链区域，另一个在末端没有单链区域，在同源重组蛋白的存在下形成。此外，根据需要，将DNA复合物引入宿主细胞，并培养所述细胞。

5. 发明授权

US06541226B1 Method for specifically cleaving double-stranded DNA and kit for the method 失效
标题翻译：用于特异性切割双链DNA的方法和该方法的试剂盒
公开(公告)号：US06541226B1
公开(公告)日：2003-04-01
申请号：US09549949
申请日：2000-04-14
申请人： Yasushi Shigemori , Michio Oishi
发明人： Yasushi Shigemori , Michio Oishi
IPC分类号： C12N1564
CPC分类号： C12N15/113 , C12N2310/15 , C12N2310/153 , C12Q1/6839 , C12Y301/30001 , C12Q2527/125 , C12Q2521/507 , C12Q2521/307
摘要： A method for specifically cleaving a double-stranded DNA. The method comprises forming a three-stranded portion comprising the double-stranded DNA portion including the position to be cleaved or its vicinity and an oligonucleotide and treating the three-stranded protion with a nuclease.
摘要翻译：一种用于特异性切割双链DNA的方法。该方法包括形成包含待切割位置或其附近的双链DNA部分的三链部分和寡核苷酸，并用核酸酶处理三链引物。

6. 发明申请

US20100125131A1 Gene Encoding A Guanine Nucleotide Exchange Factor And The Gene Product Thereof 失效
标题翻译：基因编码鸟嘌呤核苷酸交换因子及其基因产物
公开(公告)号：US20100125131A1
公开(公告)日：2010-05-20
申请号：US12651145
申请日：2009-12-31
申请人： Osamu Ohara , Takahiro Nagase , Michio Oishi , Hiroshi Yokota , Osamu Kamida
发明人： Osamu Ohara , Takahiro Nagase , Michio Oishi , Hiroshi Yokota , Osamu Kamida
IPC分类号： C07K16/00
CPC分类号： A61K31/7088 , C07K14/4702 , C07K14/82 , G01N33/57446 , G01N33/57484 , G01N2333/4703 , Y10S435/81 , Y10S435/975
摘要： A gene encoding a novel protein that works as a guanine nucleotide exchange factor (GEF) for a Rho family protein being one group of small GTP-binding proteins, namely, a polynucleotide shown by the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5, or the complementary strand, the equivalents of the polynucleotide, a protein encoded by the polynucleotide, a vector containing the polynucleotide, a transformant containing the vector, an antibody against the protein encoded by the polynucleotide, a method of identifying a compound that inhibits the function of the protein encoded by the polynucleotide and/or the expression of the polynucleotide, a method of determining a disease, a pharmaceutical composition, and a reagent kit are provided.
摘要翻译：编码作为Rho家族蛋白质的鸟嘌呤核苷酸交换因子（GEF）的新型蛋白质的基因是一组小的GTP结合蛋白，即由SEQ ID NO：1所示的核苷酸序列显示的多核苷酸， SEQ ID NO：3或SEQ ID NO：5，或互补链，多核苷酸的等同物，由多核苷酸编码的蛋白质，含有多核苷酸的载体，含有该载体的转化体，提供多核苷酸，鉴定抑制多核苷酸编码的蛋白质的功能的化合物的方法和/或多核苷酸的表达，确定疾病的方法，药物组合物和试剂盒的方法。

7. 发明申请

US20070128645A1 Use of KIAA0172 gene in treatment and diagnosis of diseases as well as in pharmaceutical development 审中-公开
标题翻译：使用KIAA0172基因治疗和诊断疾病以及药物开发
公开(公告)号：US20070128645A1
公开(公告)日：2007-06-07
申请号：US11606148
申请日：2006-11-30
申请人： Ryoichi Kiyama , Keisuke Kitajima , Shinobu Oguchi , Michio Oishi , Osamu Ohara , Takahiro Nagase
发明人： Ryoichi Kiyama , Keisuke Kitajima , Shinobu Oguchi , Michio Oishi , Osamu Ohara , Takahiro Nagase
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6886 , C07K14/47 , C12Q2600/154 , C12Q2600/172 , G01N33/505
摘要： An object of the present invention is to provide use of the KIAA0172 gene in treatment and diagnosis of diseases as well as in pharmaceutical development. An agent for treating cancer which comprises as an active ingredient a polypeptide encoded by the KIAA0172 gene, a partial sequence thereof or a variant thereof; an agent for treating cancer which comprises as an active ingredient an oligonucleotide including the KIAA0172 gene sequence; an agent for detecting cancer which comprises an antibody which recognizes polypeptide encoded by the KIAA0172 gene; an agent for detecting cancer which comprises an oligonucleotide including the KIAA0172 gene sequence; a composition for treating cancer which comprises said agent for treating cancer and a pharmaceutically acceptable carrier; and a composition for detecting cancer which comprises said agent for detecting cancer and a pharmaceutically acceptable carrier.
摘要翻译：本发明的目的是提供KIAA0172基因在疾病和药物开发的治疗和诊断中的用途。一种用于治疗癌症的药物，其包含作为活性成分的由KIAA0172基因编码的多肽，其部分序列或其变体; 一种治疗癌症的药物，其包含作为活性成分的包含KIAA0172基因序列的寡核苷酸; 用于检测癌症的试剂，其包含识别由KIAA0172基因编码的多肽的抗体; 用于检测癌症的试剂，其包含含有KIAA0172基因序列的寡核苷酸; 用于治疗癌症的组合物，其包含所述治疗癌症的药物和药学上可接受的载体; 和用于检测癌症的组合物，其包含所述用于检测癌症的药物和药学上可接受的载体。

8. 发明授权

US07667013B2 Gene encoding a guanine nucleotide exchange factor and the gene product thereof 失效
标题翻译：编码鸟嘌呤核苷酸交换因子的基因及其基因产物
公开(公告)号：US07667013B2
公开(公告)日：2010-02-23
申请号：US10594707
申请日：2005-03-29
申请人： Osamu Ohara , Takahiro Nagase , Michio Oishi , Hiroshi Yokota , Osamu Kamida
发明人： Osamu Ohara , Takahiro Nagase , Michio Oishi , Hiroshi Yokota , Osamu Kamida
IPC分类号： C12N15/11
CPC分类号： A61K31/7088 , C07K14/4702 , C07K14/82 , G01N33/57446 , G01N33/57484 , G01N2333/4703 , Y10S435/81 , Y10S435/975
摘要： A gene encoding a novel protein that works as a guanine nucleotide exchange factor (GEF) for a Rho family protein being one group of small GTP-binding proteins, namely, a polynucleotide shown by the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5, or the complementary strand, the equivalents of the polynucleotide, a protein encoded by the polynucleotide, a vector containing the polynucleotide, a transformant containing the vector, an antibody against the protein encoded by the polynucleotide, a method of identifying a compound that inhibits the function of the protein encoded by the polynucleotide and/or the expression of the polynucleotide, a method of determining a disease, a pharmaceutical composition, and a reagent kit are provided.
摘要翻译：编码作为Rho家族蛋白质的鸟嘌呤核苷酸交换因子（GEF）的新型蛋白质的基因是一组小的GTP结合蛋白，即由SEQ ID NO：1所示的核苷酸序列显示的多核苷酸， SEQ ID NO：3或SEQ ID NO：5，或互补链，多核苷酸的等同物，由多核苷酸编码的蛋白质，含有多核苷酸的载体，含有该载体的转化体，提供多核苷酸，鉴定抑制由多核苷酸编码的蛋白质的功能和/或多核苷酸表达的化合物的方法，确定疾病的方法，药物组合物和试剂盒。

9. 发明授权

US07220548B2 Partial homologous recombination of DNA chain 失效
标题翻译： DNA链的部分同源重组
公开(公告)号：US07220548B2
公开(公告)日：2007-05-22
申请号：US10798750
申请日：2004-03-10
申请人： Kazuhiro Kondo , Michio Oishi , Osamu Ohara
发明人： Kazuhiro Kondo , Michio Oishi , Osamu Ohara
IPC分类号： C12Q1/68
CPC分类号： C12N15/1082 , C12N15/102 , C12Q1/6858 , C12Q2521/507 , C12Q2537/119 , C12Q2521/319
摘要： The present invention provides a method of constructing a circular DNA library having an increased content of a desired first dsDNA by removing a second dsDNA using RecA protein to introduce a target single strand nucleic acid by homologous recombination at the 3′ terminal portion of the second dsDNA, whereby the target DNA has a 3′ terminal portion that differs from the 3′ terminal portion of the second dsDNA to prevent circularization, thereby creating a triple stranded DNA portion at the 3′ terminal end of the second dsDNA, adding Exonuclease I to digest the displaced first strand of the second dsDNA, ligating the DNA fragments to circularize the desired first dsDNA, removing the linear second dsDNA, thereby constructing the circularized DNA library having an increased content of the desired first dsDNA.
摘要翻译：本发明提供了一种通过使用RecA蛋白去除第二个双链DNA来构建所需第一个dsDNA含量增加的环状DNA文库的方法，以通过在第二个双链DNA的3'端部分同源重组引入靶单链核酸由此目标DNA具有与第二dsDNA的3'末端部分不同的3'末端部分以防止环化，从而在第二个dsDNA的3'末端产生三链DNA部分，加入外切核酸酶I进行消化连接DNA片段以使所需的第一个dsDNA环化，除去线性第二个dsDNA，从而构建具有增加的所需第一个dsDNA含量的环化的DNA文库。

10. 发明申请

US20050202431A1 Application of kiaa0172 gene to treatment and diagnosis of diseases and drug discovery 审中-公开
标题翻译：应用kiaa0172基因治疗和诊断疾病和药物发现
公开(公告)号：US20050202431A1
公开(公告)日：2005-09-15
申请号：US10509738
申请日：2002-07-26
申请人： Ryoichi Kiyama , Keisuke Kitajima , Shinobu Oguchi , Michio Oishi , Osamu Ohara , Takahiro Nagase
发明人： Ryoichi Kiyama , Keisuke Kitajima , Shinobu Oguchi , Michio Oishi , Osamu Ohara , Takahiro Nagase
IPC分类号： A61P35/00 , A61P43/00 , C07K14/47 , C12Q1/68 , G01N33/50 , A61K38/17 , C07H21/04 , C07K14/82 , C12N15/09 , G01N33/574
CPC分类号： C12Q1/6886 , C07K14/47 , C12Q2600/154 , C12Q2600/172 , G01N33/505
摘要： An object of the present invention is to provide use of the KIAA0172 gene in treatment and diagnosis of diseases as well as in pharmaceutical development. An agent for treating cancer which comprises as an active ingredient a polypeptide encoded by the KIAA0172 gene, a partial sequence thereof or a variant thereof; an agent for treating cancer which comprises as an active ingredient an oligonucleotide including the KIAA0172 gene sequence; an agent for detecting cancer which comprises an antibody which recognizes polypeptide encoded by the KIAA0172 gene; an agent for detecting cancer which comprises an oligonucleotide including the KIAA0172 gene sequence; a composition for treating cancer which comprises said agent for treating cancer and a pharmaceutically acceptable carrier; and a composition for detecting cancer which comprises said agent for detecting cancer and a pharmaceutically acceptable carrier.
摘要翻译：本发明的目的是提供KIAA0172基因在疾病和药物开发的治疗和诊断中的用途。一种用于治疗癌症的药物，其包含作为活性成分的由KIAA0172基因编码的多肽，其部分序列或其变体; 一种治疗癌症的药物，其包含作为活性成分的包含KIAA0172基因序列的寡核苷酸; 用于检测癌症的试剂，其包含识别由KIAA0172基因编码的多肽的抗体; 用于检测癌症的试剂，其包含含有KIAA0172基因序列的寡核苷酸; 用于治疗癌症的组合物，其包含所述治疗癌症的药物和药学上可接受的载体; 和用于检测癌症的组合物，其包含所述用于检测癌症的药物和药学上可接受的载体。

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