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    • 7. 发明授权
    • Structure and expression of an arabidopsis acetyl-coenzyme A carboxylase
gene
    • 拟南芥乙酰辅酶A羧化酶基因的结构和表达
    • US5962767A
    • 1999-10-05
    • US790519
    • 1997-01-29
    • John B. OhlroggeKeith R. RoeslerBasil S. Shorrosh
    • John B. OhlroggeKeith R. RoeslerBasil S. Shorrosh
    • C12N9/00C12N15/82A01H5/00A01H5/10C12N5/04
    • C12N9/93C12N15/8247
    • An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10 kb gene encodes a 251 kD cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.
    • 拟南芥乙酰辅酶A羧化酶(ACCase)基因已被分离并测序。 10 kb基因编码251 kD胞质ACCase同工酶。 该基因的核酸序列如SEQ ID No.1所示,并符合GenBank登录号L27074。 cDNA的cDNA序列和推定的氨基酸序列分别列于SEQ ID No.2和3。 通过控制本发明的基因的表达,可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此,通过在正义或反义方向引入本发明基因的构建体,可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此,也可以控制依赖于丙二酰辅酶A的植物和种子中的脂肪酸合成和延伸。
    • 8. 发明授权
    • Methods of increasing oil content of seeds
    • 增加种子油含量的方法
    • US5925805A
    • 1999-07-20
    • US677010
    • 1996-07-01
    • John B. OhlroggeKeith R. RoeslerBasil S. Shorrosh
    • John B. OhlroggeKeith R. RoeslerBasil S. Shorrosh
    • C12N9/00C12N15/82A01H5/00
    • C12N15/8247C12N9/93
    • An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10-kb gene encodes a 251-kd cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.
    • 拟南芥乙酰辅酶A羧化酶(ACCase)基因已被分离并测序。 10-kb基因编码251-kd胞质ACCase同工酶。 该基因的核酸序列如SEQ ID No.1所示,并符合GenBank登录号L27074。 cDNA的cDNA序列和推定的氨基酸序列分别列于SEQ ID No.2和3。 通过控制本发明的基因的表达,可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此,通过在正义或反义方向引入本发明基因的构建体,可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此,也可以控制依赖于丙二酰辅酶A的植物和种子中的脂肪酸合成和延伸。